Affiliation: Stanford University
- Cholesterol depletion suppresses the translational diffusion of class II major histocompatibility complex proteins in the plasma membraneMarija Vrljic
Biophysics Program, Stanford University, Stanford, California 94305 5080, USA
Biophys J 88:334-47. 2005..That is, cholesterol extraction destroys liquid cholesterol-phospholipid complexes, leaving solid-like high melting phospholipid domains that inhibit the lateral diffusion of membrane components...
- Cholesterol depletion induces solid-like regions in the plasma membraneStefanie Y Nishimura
Department of Chemistry, Molecular and Cellular Physiology, and Biophysics Program, Stanford University, Stanford, California 94305 5080, USA
Biophys J 90:927-38. 2006..Cytoskeletal effects appear to be minimal. These results are consistent with a previously described model of solid-like domain formation in the plasma membrane...
- Translational diffusion of individual class II MHC membrane proteins in cellsMarija Vrljic
Biophysics Program, Stanford University, Stanford, CA 94305 5080, USA
Biophys J 83:2681-92. 2002..Both analyses show that motion is predominantly Brownian. This study finds no strong evidence for significant confinement of either GPI-linked or native I-E(k) in the plasma membrane of CHO cells...
- Three-dimensional molecular modeling with single molecule FRETAxel T Brunger
The Howard Hughes Medical Institute, Stanford University, CA 94305, USA
J Struct Biol 173:497-505. 2011....
- Single-molecule trackingMarija Vrljic
Dept of Chemistry, Stanford University, CA 94305, USA
Methods Mol Biol 398:193-219. 2007....
- Synaptic proteins promote calcium-triggered fast transition from point contact to full fusionJiajie Diao
Departments of Molecular and Cellular Physiology, Neurology and Neurological Sciences, Structural Biology, Photon Science and Howard Hughes Medical Institute, Stanford University, Stanford, USA
elife 1:e00109. 2012..Synaptic proteins may have evolved to select this immediate pathway out of a heterogeneous network of possible membrane fusion pathways.DOI:http://dx.doi.org/10.7554/eLife.00109.001...
- In vitro system capable of differentiating fast Ca2+-triggered content mixing from lipid exchange for mechanistic studies of neurotransmitter releaseMinjoung Kyoung
Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, 318 Campus Drive West, Stanford, CA 94305 5432, USA
Proc Natl Acad Sci U S A 108:E304-13. 2011....
- Post-translational modifications and lipid binding profile of insect cell-expressed full-length mammalian synaptotagmin 1Marija Vrljic
Department of Molecular and Cellular Physiology, Stanford University, Stanford, California 94305 5432, USA
Biochemistry 50:9998-10012. 2011..These results suggest a conserved lipid binding mechanism in which Ca(2+)-independent interactions are mediated via a lysine rich region of the C2B domain while Ca(2+)-dependent interactions are mediated via the Ca(2+)-binding loops...
- Molecular mechanism of the synaptotagmin-SNARE interaction in Ca2+-triggered vesicle fusionMarija Vrljic
Department of Molecular and Cellular Physiology, Stanford University, Stanford, California, USA
Nat Struct Mol Biol 17:325-31. 2010..The loops resemble the membrane-interacting loops of certain viral fusion proteins in the postfusion state, suggesting unexpected similarities between both fusion systems...
- Liquid-liquid immiscibility in membranesHarden M McConnell
Department of Chemistry, Biophysics Program, Stanford University, Stanford, California 94305 5080, USA
Annu Rev Biophys Biomol Struct 32:469-92. 2003..The properties of defined cholesterol-phospholipid mixtures provide a conceptual foundation for the exploration of a number of aspects of the biophysics and biochemistry of animal cell membranes...
- The structure of the yeast plasma membrane SNARE complex reveals destabilizing water-filled cavitiesPavel Strop
Howard Hughes Medical Institute and Department of Molecular and Cellular Physiology, Stanford University, Stanford, California 94305, USA
J Biol Chem 283:1113-9. 2008..Mutagenesis experiments suggest that the water-filled cavities contribute to the lower stability of the S. cerevisiae complex...
- Ensemble molecular dynamics yields submillisecond kinetics and intermediates of membrane fusionPeter M Kasson
Medical Scientist Training and Biophysics Programs, Department of Computer Science, Stanford Synchrotron Radiation Laboratory, Stanford University, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 103:11916-21. 2006....
- Both MHC class II and its GPI-anchored form undergo hop diffusion as observed by single-molecule trackingYasuhiro M Umemura
Membrane Mechanisms Project, International Cooperative Research Project, Japan Science and Technology Agency, Institute for Integrated Cell Material Sciences, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
Biophys J 95:435-50. 2008..These results indicate that the actin-based membrane skeleton influences the diffusion of both transmembrane and GPI-anchored proteins...