Genomes and Genes
Affiliation: Stanford University
- Oxysterols are allosteric activators of the oncoprotein SmoothenedSigrid Nachtergaele
Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA
Nat Chem Biol 8:211-20. 2012..Our work suggests that these enigmatic sterols, which have multiple effects on cell physiology, may act as ligands for signaling receptors and provides a generally applicable framework for probing sterol signaling mechanisms...
- The ciliary membraneRajat Rohatgi
Department of Medicine, Stanford University School of Medicine, 279 Campus Drive, Stanford, CA 94305, USA
Curr Opin Cell Biol 22:541-6. 2010..The regulated movement of membrane proteins and lipids across this barrier is central to the sensory function of these organelles...
- Patched1 regulates hedgehog signaling at the primary ciliumRajat Rohatgi
Departments of Developmental Biology, Genetics, and Bioengineering and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA
Science 317:372-6. 2007..When Shh bound to Ptc1, Ptc1 left the cilia, leading to accumulation of Smo and activation of signaling. Thus, primary cilia sense Shh and transduce signals that play critical roles in development, carcinogenesis, and stem cell function...
- Lateral transport of Smoothened from the plasma membrane to the membrane of the ciliumLjiljana Milenkovic
Howard Hughes Medical Institute, Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
J Cell Biol 187:365-74. 2009..We anticipate that this alternative route will be used by other signaling proteins that function at cilia. The path taken by Smo may allow novel strategies for modulation of Hh signaling in cancer and regeneration...
- The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteinsEric W Humke
Division of Oncology, Department of Medicine, Stanford University School of Medicine, Stanford, California 94305, USA
Genes Dev 24:670-82. 2010..We propose that the Sufu-Gli3 interaction is a major control point in the Hedgehog pathway, a pathway that plays important roles in both development and cancer...
- EFCAB7 and IQCE regulate hedgehog signaling by tethering the EVC-EVC2 complex to the base of primary ciliaGanesh V Pusapati
Departments of Biochemistry and Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
Dev Cell 28:483-96. 2014..Evolutionary analysis suggests that emergence of these complexes might have been important for adaptation of an ancient organelle, the cilium, for an animal-specific signaling network...
- A Smoothened-Evc2 complex transduces the Hedgehog signal at primary ciliaKarolin V Dorn
Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
Dev Cell 23:823-35. 2012..Our data suggest that the Smo-Evc2 signaling complex at the EvC zone is required for Hh signal transmission and elucidate the molecular basis of two human ciliopathies...
- Patching the gaps in Hedgehog signallingRajat Rohatgi
Department of Developmental Biology, Howard Hughes Medical Institute, Clark Center West W252, 318 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305 5439, USA
Nat Cell Biol 9:1005-9. 2007....
- Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signalingGiovanni Luchetti
Department of Biochemistry, Stanford University School of Medicine, Stanford, United States
elife 5:. 2016..Our results show that cholesterol can initiate signaling from the cell surface by engaging the extracellular domain of a GPCR and suggest that SMO activity may be regulated by local changes in cholesterol abundance or accessibility...
- Hedgehog signal transduction by Smoothened: pharmacologic evidence for a 2-step activation processRajat Rohatgi
Department of Developmental Biology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 106:3196-201. 2009..This 2-step mechanism--localization followed by activation--has direct implications for the design and use of anticancer therapeutics targeted against Smo...
- Cell biology. Arrestin' movement in ciliaRajat Rohatgi
Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305 5439, USA
Science 320:1726-7. 2008
- Cancer risk after use of recombinant bone morphogenetic protein-2 for spinal arthrodesisEugene J Carragee
Department of Orthopaedic Surgery, Stanford University School of Medicine, 450 Broadway Street, Pavilion C, 4th Floor, Redwood City, CA 94063, USA
J Bone Joint Surg Am 95:1537-45. 2013..The effect of rhBMP-2 on the risk of cancer has been a concern. We sought to evaluate the risk of new cancers in patients receiving high-dose rhBMP-2...
- Isolation and mutational analysis of circulating tumor cells from lung cancer patients with magnetic sifters and biochipsChristopher M Earhart
Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA
Lab Chip 14:78-88. 2014..The use of these magnetic technologies, which are separate devices, may lead the way to routine preparation and characterization of "liquid biopsies" from cancer patients. ..
- Gli protein activity is controlled by multisite phosphorylation in vertebrate Hedgehog signalingPawel Niewiadomski
Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA Department of Cell Biology, Nencki Institute of Experimental Biology, 02 093 Warsaw, Poland Electronic address
Cell Rep 6:168-81. 2014....
- Biochemical Mechanisms of Hedgehog SignalingRajat Rohatgi; Fiscal Year: 2008..Most importantly, it will put me in an ideal position for a tenure-track position in an oncology department, where I plan to spend ~80-90% of my time in research and teaching and 10-20% of my time in the care of cancer patients. ..