Genomes and Genes
Jason W Myers
Affiliation: Stanford University
- The MAPK kinase kinase-1 is essential for stress-induced pancreatic islet cell deathDariush Mokhtari
Department of Medical Cell Biology, Uppsala University, Biomedicum, S 751 23 Uppsala, Sweden
Endocrinology 149:3046-53. 2008..Increased understanding of the signaling pathways that augment or diminish beta-cell MEKK-1 activity may aid in the generation of novel therapeutic strategies in the treatment of type 1 diabetes...
- Minimizing off-target effects by using diced siRNAs for RNA interferenceJason W Myers
Stanford University School of Medicine, Department of Molecular Pharmacology, 269 West Campus Drive, CCSR Room 3155, Stanford CA 94305 5174, USA
J RNAi Gene Silencing 2:181-94. 2006..elegans and D. melanogaster, where gene suppression is mediated by endogenously-generated diced siRNA pools, and provide a strategy for improving the specificity of RNA interference experiments and screens in mammalian cells...
- Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencingJason W Myers
Department of Molecular Pharmacology, 269 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305 5174, USA
Nat Biotechnol 21:324-8. 2003..Dicer-generated siRNAs (d-siRNAs) are effective in silencing transiently transfected reporter genes and endogenous genes, making in vitro dicing a useful, practical alternative for the production of siRNAs...
- siRNA screen of the human signaling proteome identifies the PtdIns(3,4,5)P3-mTOR signaling pathway as a primary regulator of transferrin uptakeThierry Galvez
Department of Chemical and Systems Biology and Bio X Program, Stanford University School of Medicine, Stanford, CA 94305, USA
Genome Biol 8:R142. 2007..Signaling pathways that regulate transferrin uptake have not yet been identified...
- Cyclin A2 regulates nuclear-envelope breakdown and the nuclear accumulation of cyclin B1Delquin Gong
Department of Chemical and Systems Biology, CCSR, MC 5174, Stanford University School of Medicine, Stanford, California 94305, USA
Curr Biol 17:85-91. 2007..Nevertheless cyclin B1 translocates to the nucleus just prior to NEB in a cyclin A2-dependent fashion and is capable of supporting NEB if rendered constitutively nuclear...
- Silencing gene expression with Dicer-generated siRNA poolsJason W Myers
Department of Molecular Pharmacology, Stanford University Medical School, CA, USA
Methods Mol Biol 309:93-196. 2005
- STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered Ca2+ influxJen Liou
Department of Molecular Pharmacology, Stanford University Medical School, California 94305, USA
Curr Biol 15:1235-41. 2005..Our study suggests that STIM proteins function as Ca(2+) store sensors in the signaling pathway connecting Ca(2+) store depletion to Ca(2+) influx...
- Probing the precision of the mitotic clock with a live-cell fluorescent biosensorJoshua T Jones
Department of Molecular Pharmacology, W200 Clark, 318 Campus Drive, Stanford University Medical School, Stanford, California 94305, USA
Nat Biotechnol 22:306-12. 2004..Inactivation of the spindle checkpoint by targeting the regulator Mad2 with RNAi consistently shortened mitosis, providing direct evidence that the internal mitotic timing mechanism is much faster in cells that lack the checkpoint...
- Selective regulation of neurite extension and synapse formation by the beta but not the alpha isoform of CaMKIICharles C Fink
Department of Molecular Pharmacology, Stanford University Medical School, Stanford, CA 94305, USA
Neuron 39:283-97. 2003..These results show that the two main neuronal CaMKII isoforms have markedly different roles in neuronal plasticity, with CaMKIIalpha regulating synaptic strength and CaMKIIbeta controlling the dendritic morphology and number of synapses...
- Concordant regulation of translation and mRNA abundance for hundreds of targets of a human microRNADavid G Hendrickson
Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, California, USA
PLoS Biol 7:e1000238. 2009....
- Restriction enzyme-generated siRNA (REGS) vectors and librariesGeorge Sen
Department of Molecular Pharmacology, 269 Campus Drive, CCSR 4225A Stanford University School of Medicine, Stanford, California 94305, USA
Nat Genet 36:183-9. 2004..REGS enabled us to create enzymatically a complex siRNA library (>4 x 10(5) clones) from double-stranded cDNA encompassing known and unknown genes with 96% of the clones containing inserts of the appropriate size...
- siRNA produced by recombinant dicer mediates efficient gene silencing in islet cellsRobert Hägerkvist
Department of Medical Cell Biology, Uppsala University Biomedicum, Sweden
Ann N Y Acad Sci 1040:114-22. 2005..The approach described here using d-siRNA provides an important tool for elucidating gene function in further studies of pancreatic islets and diabetes pathophysiology...
- MAPK kinase kinase-1 is essential for cytokine-induced c-Jun NH2-terminal kinase and nuclear factor-kappaB activation in human pancreatic islet cellsDariush Mokhtari
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden
Diabetes 57:1896-904. 2008..The aim of the present investigation was to elucidate a putative role of the MAPK kinase kinase-1 (MEKK-1) in cytokine-induced signaling...
- Transforming growth factor-beta-activated protein kinase 1-binding protein (TAB)-1alpha, but not TAB1beta, mediates cytokine-induced p38 mitogen-activated protein kinase phosphorylation and cell death in insulin-producing cellsNatalia Makeeva
Department of Medical Cell Biology, Uppsala University, S 75123 Uppsala, Sweden
Endocrinology 149:302-9. 2008..We conclude that TAB1alpha, but not TAB1beta, plays an important role in the activation of p38 in insulin-producing cells and therefore also in cytokine-induced beta-cell death...