Affiliation: Stanford University
- An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathwaysTakanari Inoue
Stanford University, Department of Molecular Pharmacology, Clark Center, 318 Campus Drive, Stanford, California 94305 5439, USA
Nat Methods 2:415-8. 2005..As signaling processes often occur within minutes, such rapid perturbations provide a powerful tool to investigate the role, selectivity and timing of Rho GTPase-mediated signaling processes...
- PI(3,4,5)P3 and PI(4,5)P2 lipids target proteins with polybasic clusters to the plasma membraneWon Do Heo
Department of Molecular Pharmacology, 318 Campus Drive, Clark Building, Stanford University Medical School, Stanford, CA 94305, USA
Science 314:1458-61. 2006....
- Dissecting the role of PtdIns(4,5)P2 in endocytosis and recycling of the transferrin receptorNamiko Abe
Graduate Program in Neurosciences, Stanford University School of Medicine, Stanford, CA 94305, USA
J Cell Sci 121:1488-94. 2008..This argues that receptor-mediated PtdIns(4,5)P(2) reduction preferentially suppresses AP-2-mediated targeting of cargo to endocytic sites rather than the assembly of clathrin coats or recycling of endocytic vesicles...
- Synthetic activation of endogenous PI3K and Rac identifies an AND-gate switch for cell polarization and migrationTakanari Inoue
Chemical and Systems Biology, Bio X Program, Stanford University, Stanford, California, USA
PLoS ONE 3:e3068. 2008..This AND-gate control for cell polarization can explain how Rac can be employed for both PI3K-dependent and -independent signaling pathways coexisting in the same cell...
- Robust neuronal symmetry breaking by Ras-triggered local positive feedbackMarc Fivaz
Clark Center, Bio X, Department of Chemical and Systems Biology, Stanford University, 318 Campus Drive, Stanford, California 94305, USA
Curr Biol 18:44-50. 2008....
- Live-cell imaging reveals sequential oligomerization and local plasma membrane targeting of stromal interaction molecule 1 after Ca2+ store depletionJen Liou
Department of Chemical and Systems Biology, Stanford University Medical School, 318 Campus Drive, Clark Center, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 104:9301-6. 2007....
- Rapid chemically induced changes of PtdIns(4,5)P2 gate KCNQ ion channelsByung Chang Suh
Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, WA 98195, USA
Science 314:1454-7. 2006..Hence, the depletion of PI(4,5)P2 suffices to suppress current fully, and other second messengers are not needed. Our approach is ideally suited to study biological signaling networks involving membrane phosphoinositides...
- An essential role for the SHIP2-dependent negative feedback loop in neuritogenesis of nerve growth factor-stimulated PC12 cellsKazuhiro Aoki
Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
J Cell Biol 177:817-27. 2007....
- Selective photoinactivation of protein function through environment-sensitive switching of singlet oxygen generation by photosensitizerTakatoshi Yogo
Graduate School of Pharmaceutical Sciences and Department of Pharmacology, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
Proc Natl Acad Sci U S A 105:28-32. 2008....
- Modification of intracellular Ca2+ dynamics by laser inactivation of inositol 1,4,5-trisphosphate receptor using membrane-permeant probesTakatoshi Yogo
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
Chem Biol 11:1053-8. 2004..These results demonstrate the potency of the smCALI technique for the study of the roles of IP3R in complex intracellular Ca2+ dynamics...
- Hydrophobic modifications at 1-phosphate of inositol 1,4,5-trisphosphate analogues enhance receptor bindingWaka Nakanishi
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
Bioorg Med Chem Lett 12:911-3. 2002..Our results show that hydrophobic modifications of the 1-phosphate moiety enhance the binding affinity, with considerable latitude of substituent structure...
- Feedback and Crosstalk in Eukaryotic ChemotaxisTakanari Inoue; Fiscal Year: 2010..Our unique perturbation approach can be a powerful strategy for not only dissecting the molecular mechanisms, but also interfering with these cell migration-related diseases. ..