Nathan R Treff

Summary

Affiliation: Reproductive Medicine Associates of New Jersey
Country: USA

Publications

  1. doi Accurate single cell 24 chromosome aneuploidy screening using whole genome amplification and single nucleotide polymorphism microarrays
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07960, USA
    Fertil Steril 94:2017-21. 2010
  2. doi A novel single-cell DNA fingerprinting method successfully distinguishes sibling human embryos
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07960, USA
    Fertil Steril 94:477-84. 2010
  3. doi Detection of segmental aneuploidy and mosaicism in the human preimplantation embryo: technical considerations and limitations
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey Thomas Jefferson University, Philadelphia, Pennsylvania Electronic address
    Fertil Steril . 2016
  4. doi Next Generation Sequencing-Based Comprehensive Chromosome Screening in Mouse Polar Bodies, Oocytes, and Embryos
    Nathan R Treff
    Department of Obstetrics, Gynecology, and Reproductive Sciences, Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey
    Biol Reprod 94:76. 2016
  5. pmc SNP array-based analyses of unbalanced embryos as a reference to distinguish between balanced translocation carrier and normal blastocysts
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Basking Ridge, NJ, 07920, USA
    J Assist Reprod Genet 33:1115-9. 2016
  6. pmc Incidental identification of balanced translocation carrier patients through comprehensive chromosome screening of IVF-derived blastocysts
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA
    J Assist Reprod Genet 30:787-91. 2013
  7. doi Evaluation of targeted next-generation sequencing-based preimplantation genetic diagnosis of monogenic disease
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA
    Fertil Steril 99:1377-1384.e6. 2013
  8. doi Four-hour quantitative real-time polymerase chain reaction-based comprehensive chromosome screening and accumulating evidence of accuracy, safety, predictive value, and clinical efficacy
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07960, USA
    Fertil Steril 99:1049-53. 2013
  9. doi Genome-wide analysis of human preimplantation aneuploidy
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
    Semin Reprod Med 30:283-8. 2012
  10. doi Blastocyst preimplantation genetic diagnosis (PGD) of a mitochondrial DNA disorder
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07960, USA
    Fertil Steril 98:1236-40. 2012

Collaborators

Detail Information

Publications44

  1. doi Accurate single cell 24 chromosome aneuploidy screening using whole genome amplification and single nucleotide polymorphism microarrays
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07960, USA
    Fertil Steril 94:2017-21. 2010
    ..To develop and validate a whole genome amplification and single nucleotide polymorphism (SNP) microarray protocol for accurate single cell 24 chromosome aneuploidy screening...
  2. doi A novel single-cell DNA fingerprinting method successfully distinguishes sibling human embryos
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07960, USA
    Fertil Steril 94:477-84. 2010
    ..To validate a novel system for embryonic DNA fingerprinting which can reliably distinguish sibling embryos from each other...
  3. doi Detection of segmental aneuploidy and mosaicism in the human preimplantation embryo: technical considerations and limitations
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey Thomas Jefferson University, Philadelphia, Pennsylvania Electronic address
    Fertil Steril . 2016
    ....
  4. doi Next Generation Sequencing-Based Comprehensive Chromosome Screening in Mouse Polar Bodies, Oocytes, and Embryos
    Nathan R Treff
    Department of Obstetrics, Gynecology, and Reproductive Sciences, Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey
    Biol Reprod 94:76. 2016
    ....
  5. pmc SNP array-based analyses of unbalanced embryos as a reference to distinguish between balanced translocation carrier and normal blastocysts
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Basking Ridge, NJ, 07920, USA
    J Assist Reprod Genet 33:1115-9. 2016
    ..The purpose of the study is to validate a method that provides the opportunity to distinguish a balanced translocation carrier embryo from a truly normal embryo in parallel with comprehensive chromosome screening (CCS)...
  6. pmc Incidental identification of balanced translocation carrier patients through comprehensive chromosome screening of IVF-derived blastocysts
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA
    J Assist Reprod Genet 30:787-91. 2013
    ..To demonstrate that translocation carrier patients can be identified by analysis of chromosomes in preimplantation human embryos...
  7. doi Evaluation of targeted next-generation sequencing-based preimplantation genetic diagnosis of monogenic disease
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA
    Fertil Steril 99:1377-1384.e6. 2013
    ..To investigate the applicability of next-generation sequencing (NGS) to preimplantation genetic diagnosis (PGD); to evaluate semiconductor-based NGS for genetic analysis of human embryos...
  8. doi Four-hour quantitative real-time polymerase chain reaction-based comprehensive chromosome screening and accumulating evidence of accuracy, safety, predictive value, and clinical efficacy
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07960, USA
    Fertil Steril 99:1049-53. 2013
    ..This Biomarkers in Reproductive Medicine special issue review summarizes the accumulated experience with the development and clinical application of a 4-hour blastocyst qPCR-based CCS technology...
  9. doi Genome-wide analysis of human preimplantation aneuploidy
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
    Semin Reprod Med 30:283-8. 2012
    ..This review will provide an overview of the basic underlying features and applications of the growing number of platforms and strategies for preimplantation genome-wide analysis of aneuploidy...
  10. doi Blastocyst preimplantation genetic diagnosis (PGD) of a mitochondrial DNA disorder
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07960, USA
    Fertil Steril 98:1236-40. 2012
    ..To evaluate the utility of trophectoderm biopsy for preimplantation genetic diagnosis (PGD) of mitochondrial (mt) DNA mutation load...
  11. pmc Methods for comprehensive chromosome screening of oocytes and embryos: capabilities, limitations, and evidence of validity
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA
    J Assist Reprod Genet 29:381-90. 2012
    ..This article will focus on reviewing these technologies and the evidence of their validity...
  12. doi Development and validation of an accurate quantitative real-time polymerase chain reaction-based assay for human blastocyst comprehensive chromosomal aneuploidy screening
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
    Fertil Steril 97:819-24. 2012
    ..To develop and validate a quantitative real-time polymerase chain reaction (qPCR)-based method for blastocyst trophectoderm comprehensive chromosome screening (CCS) of aneuploidy...
  13. pmc Polar body morphology is not predictive of its cell division origin
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA
    J Assist Reprod Genet 29:137-9. 2012
    ..This study sought to determine whether morphology of simultaneously biopsied polar bodies is predictive of their cell division origin...
  14. pmc Telomere DNA deficiency is associated with development of human embryonic aneuploidy
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, United States of America
    PLoS Genet 7:e1002161. 2011
    ..Heterogeneity in telomere length within oocytes may provide an opportunity to improve the treatment of infertility through telomere-based selection of oocytes and embryos with reproductive competence...
  15. doi Robust embryo identification using first polar body single nucleotide polymorphism microarray-based DNA fingerprinting
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
    Fertil Steril 93:2453-5. 2010
    ..First polar body-based assignments of which embryos implanted and were delivered after multiple ET were 100% consistent with previously validated embryo DNA fingerprinting-based assignments...
  16. pmc SNP microarray-based 24 chromosome aneuploidy screening is significantly more consistent than FISH
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA
    Mol Hum Reprod 16:583-9. 2010
    ..These results also suggest that FISH technology may overestimate the contribution of mitotic error to the origin of aneuploidy at the cleavage stage of human embryogenesis...
  17. doi Single nucleotide polymorphism microarray-based concurrent screening of 24-chromosome aneuploidy and unbalanced translocations in preimplantation human embryos
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07960, USA
    Fertil Steril 95:1606-12.e1-2. 2011
    ....
  18. doi Use of single nucleotide polymorphism microarrays to distinguish between balanced and normal chromosomes in embryos from a translocation carrier
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey Research, 111 Madison Avenue, Suite 100, Morristown, NJ 07960, USA
    Fertil Steril 96:e58-65. 2011
    ..To prove the ability to distinguish between balanced and normal chromosomes in embryos from a translocation carrier...
  19. pmc Single-cell whole-genome amplification technique impacts the accuracy of SNP microarray-based genotyping and copy number analyses
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA
    Mol Hum Reprod 17:335-43. 2011
    ..Alternatively, for applications that do not have constraints on turnaround time and that are directed at accurate genotyping rather than CN assignments, a REPLI-g-based protocol may provide the best solution...
  20. doi Tracking embryo implantation using cell-free fetal DNA enriched from maternal circulation at 9 weeks gestation
    Nathan R Treff
    Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA
    Mol Hum Reprod 17:434-8. 2011
    ..This study demonstrates the first validated fetal DNA fingerprinting method which predicts both gender and which embryo implanted at 9 weeks gestation following multiple embryo transfer...
  21. doi Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery rates: a randomized controlled trial
    Richard T Scott
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
    Fertil Steril 100:697-703. 2013
    ..To determine whether blastocyst biopsy and rapid quantitative real-time polymerase chain reaction (qPCR)-based comprehensive chromosome screening (CCS) improves in vitro fertilization (IVF) implantation and delivery rates...
  22. doi Comprehensive chromosome screening is highly predictive of the reproductive potential of human embryos: a prospective, blinded, nonselection study
    Richard T Scott
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07960, USA
    Fertil Steril 97:870-5. 2012
    ..To determine both the negative and positive predictive values of comprehensive chromosome screening (CCS) results for clinical outcome...
  23. pmc Delivery of a chromosomally normal child from an oocyte with reciprocal aneuploid polar bodies
    Richard T Scott
    Reproductive Medicine Associates of New Jersey, 111 Madison Ave, Morristown, NJ 07960, USA
    J Assist Reprod Genet 29:533-7. 2012
    ..To demonstrate that a euploid embryo derived from an oocyte with reciprocal aneuploid polar bodies is capable of producing a chromosomally normal child...
  24. doi Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: a randomized and paired clinical trial
    Richard T Scott
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07920, USA
    Fertil Steril 100:624-30. 2013
    ..To determine if cleavage- or blastocyst-stage embryo biopsy affects reproductive competence...
  25. doi In vitro fertilization with single euploid blastocyst transfer: a randomized controlled trial
    Eric J Forman
    Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey 07920, USA
    Fertil Steril 100:100-7.e1. 2013
    ....
  26. doi Oocyte vitrification does not increase the risk of embryonic aneuploidy or diminish the implantation potential of blastocysts created after intracytoplasmic sperm injection: a novel, paired randomized controlled trial using DNA fingerprinting
    Eric J Forman
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
    Fertil Steril 98:644-9. 2012
    ..To assess the impact of oocyte vitrification on aneuploidy and reproductive potential by comparing vitrified and control oocytes from a single patient within a single cycle and a single fresh transfer...
  27. pmc High-throughput carrier screening using TaqMan allelic discrimination
    Anastasia Fedick
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, New Jersey, United States of America
    PLoS ONE 8:e59722. 2013
    ....
  28. doi Comprehensive chromosome screening and embryo selection: moving toward single euploid blastocyst transfer
    Eric J Forman
    Reproductive Medicine Associates of New Jersey, Morristown, NJ 07960, USA
    Semin Reprod Med 30:236-42. 2012
    ..This review describes the critical components of a valid CCS embryo selection methodology and discusses the limitations and opportunities for future routine application in reproductive medicine...
  29. doi Genotypically determined ancestry across an infertile population: ovarian reserve and response parameters are not influenced by continental origin
    Meir Olcha
    Division of Reproductive Endocrinology, Department of Obstetrics, Gynecology, and Reproductive Science, Robert Wood Johnson Medical School, Rutgers University, New Brunswick, New Jersey Electronic address
    Fertil Steril 106:475-80. 2016
    ....
  30. doi High relative deoxyribonucleic acid content of trophectoderm biopsy adversely affects pregnancy outcomes
    Shelby A Neal
    Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey Sidney Kimmel College of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania Electronic address
    Fertil Steril . 2016
    ..To evaluate the association between relative DNA content of the trophectoderm biopsy and pregnancy outcomes...
  31. pmc Trophectoderm DNA fingerprinting by quantitative real-time PCR successfully distinguishes sibling human embryos
    Richard T Scott
    Reproductive Medicine Associates of New Jersey, 140 Allen Rd, Basking Ridge, Morristown, NJ, 07960, USA
    J Assist Reprod Genet 31:1421-5. 2014
    ..To validate a novel and more practical system for trophectoderm DNA fingerprinting which reliably distinguishes sibling embryos from each other...
  32. doi Comprehensive chromosome screening alters traditional morphology-based embryo selection: a prospective study of 100 consecutive cycles of planned fresh euploid blastocyst transfer
    Eric J Forman
    Reproductive Medicine Associates of New Jersey, Department of Reproductive Endocrinology, Basking Ridge, New Jersey 07920, USA
    Fertil Steril 100:718-24. 2013
    ....
  33. pmc Aneuploidy across individual chromosomes at the embryonic level in trophectoderm biopsies: changes with patient age and chromosome structure
    Jason M Franasiak
    Division of Reproductive Endocrinology, Department of Obstetrics Gynecology and Reproductive Sciences, Robert Wood Johnson Medical School, Rutgers University, New Brunswick, NJ, USA
    J Assist Reprod Genet 31:1501-9. 2014
    ..To characterize each chromosome's risk for being involved in embryonic aneuploidy...
  34. pmc Lack of association of KATNAL1 gene sequence variants and azoospermia in humans
    Anastasia M Fedick
    Department of Microbiology and Molecular Genetics, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ, 08854 5635, USA
    J Assist Reprod Genet 31:1065-71. 2014
    ..This study investigated the relevance of this gene to infertility in humans...
  35. pmc A randomized and blinded comparison of qPCR and NGS-based detection of aneuploidy in a cell line mixture model of blastocyst biopsy mosaicism
    David Goodrich
    Reproductive Medicine Associates of New Jersey, 140 Allen Rd, Basking Ridge, NJ, 07920, USA
    J Assist Reprod Genet 33:1473-1480. 2016
    ..This study evaluates the sensitivity and specificity of aneuploidy detection by two CCS platforms using a cell line mixture model of a mosaic trophectoderm biopsy...
  36. doi Uniparental disomy in the human blastocyst is exceedingly rare
    Ndeye Aicha Gueye
    Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey Department of Obstetrics, Gynecology, and Reproductive Sciences, Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey
    Fertil Steril 101:232-6. 2014
    ....
  37. pmc High-throughput real-time PCR-based genotyping without DNA purification
    Anastasia Fedick
    Department of Molecular Genetics, Microbiology, and Immunology, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ, USA
    BMC Res Notes 5:573. 2012
    ..In this paper, a method of quantitative qPCR-based genotyping on blood without DNA purification was developed using a high-throughput qPCR platform...
  38. doi Fertility after age 45: From natural conception to Assisted Reproductive Technology and beyond
    Eric J Forman
    Reproductive Medicine Associates of New Jersey, 111 Madison Avenue, Suite 100, Morristown, NJ 07960, USA
    Maturitas 70:216-21. 2011
    ..Finally, attempts to reverse the age-related decline in oocyte quality through micro-manipulation of the nucleus and cytoplasm have yielded disappointing results and are fraught with ethical concerns...
  39. doi CYP1A1 3801T>C polymorphism implicated in altered xenobiotic metabolism is not associated with variations in sperm production and function as measured by total motile sperm and fertilization rates with intracytoplasmic sperm injection
    Jason M Franasiak
    Division of Reproductive Endocrinology, Department of Obstetrics, Gynecology and Reproductive Science, Robert Wood Johnson Medical School, Rutgers University, New Brunswick, New Jersey Electronic address
    Fertil Steril 106:481-6. 2016
    ....
  40. doi Combination of uterine natural killer cell immunoglobulin receptor haplotype and trophoblastic HLA-C ligand influences the risk of pregnancy loss: a retrospective cohort analysis of direct embryo genotyping data from euploid transfers
    Scott J Morin
    Reproductive Medicine Associates of New Jersey, Basking Ridge, New Jersey Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania Electronic address
    Fertil Steril . 2017
    ....
  41. doi 24-chromosome PCR for aneuploidy screening
    Marie D Werner
    aRobert Wood Johnson Medical School of Rutgers University, New Brunswick bReproductive Medicine Associates of New Jersey and Rutgers Robert Wood Johnson Medical School, Basking Ridge, New Jersey, USA
    Curr Opin Obstet Gynecol 27:201-5. 2015
    ..The purpose of this study is to describe the many advantages of PCR-based comprehensive chromosome screening (CCS) methodologies and the importance of extensive preclinical validation...
  42. doi Embryonic aneuploidy does not differ among genetic ancestry according to continental origin as determined by ancestry informative markers
    Jason M Franasiak
    Division of Reproductive Endocrinology, Department of Obstetrics, Gynecology, and Reproductive Science, Robert Wood Johnson Medical School, Rutgers University, 125 Paterson Street New Brunswick, NJ 08901, USA Reproductive Medicine Associates of New Jersey, 140 Allen Road, Basking Ridge, NJ 07920, USA
    Hum Reprod 31:2391-5. 2016
    ..Is embryonic aneuploidy, as determined by comprehensive chromosome screening (CCS), related to genetic ancestry, as determined by ancestry informative markers (AIMs)?..
  43. doi Development of TaqMan allelic discrimination based genotyping of large DNA deletions
    Anastasia Fedick
    Department of Molecular Genetics, Microbiology, and Immunology, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ, USA
    Genomics 99:127-31. 2012
    ..This methodology may also be successfully applied to high throughput genotyping of other large deletions...
  44. doi Assessing the reproductive competence of individual embryos: a proposal for the validation of new "-omics" technologies
    Richard T Scott
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey 07962, USA
    Fertil Steril 94:791-4. 2010
    ....