Rachel Friedman Ohana

Summary

Affiliation: Promega Corporation
Country: USA

Publications

  1. doi request reprint HaloTag7: a genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification
    Rachel Friedman Ohana
    Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
    Protein Expr Purif 68:110-20. 2009
  2. doi request reprint HaloTag-based purification of functional human kinases from mammalian cells
    Rachel Friedman Ohana
    Promega Corporation, Madison, WI 53711, USA
    Protein Expr Purif 76:154-64. 2011
  3. pmc Target engagement and drug residence time can be observed in living cells with BRET
    Matthew B Robers
    Promega Corporation, 2800 Woods Hollow Road, Fitchburg, Wisconsin 53711, USA
    Nat Commun 6:10091. 2015
  4. doi request reprint Deciphering the Cellular Targets of Bioactive Compounds Using a Chloroalkane Capture Tag
    Rachel Friedman Ohana
    Promega Corporation, Madison, Wisconsin, United States
    ACS Chem Biol 10:2316-24. 2015
  5. doi request reprint Purification of Recombinant Proteins from Cultured Mammalian Cells by HaloTag Technology
    Rachel Friedman Ohana
    Promega Corporation, Madison, Wisconsin
    Curr Protoc Mol Biol 110:10.31.1-15. 2015
  6. doi request reprint HaloTag: a novel protein labeling technology for cell imaging and protein analysis
    Georgyi V Los
    Promega Corporation, 2800 Woods Hollow Rd, Madison, Wisconsin 53711, USA
    ACS Chem Biol 3:373-82. 2008

Collaborators

  • Andrew L Niles
  • Georgyi V Los
  • Danette D Hartzell
  • Matthew B Robers
  • Kristopher Zimmerman
  • James R Hartnett
  • Thomas A Kirkland
  • Melanie L Dart
  • Chad A Zimprich
  • Sergiy Levin
  • Monika G Wood
  • Carolyn C Woodroofe
  • Keith V Wood
  • Danette L Daniels
  • Yi Qiang Cheng
  • Michael Slater
  • Kevin R Kupcho
  • Thomas Machleidt
  • Mei Cong

Detail Information

Publications6

  1. doi request reprint HaloTag7: a genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification
    Rachel Friedman Ohana
    Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
    Protein Expr Purif 68:110-20. 2009
    ..Using a subset of the test panel, more proteins fused to HaloTag7 were successfully purified than with the other tags, and these proteins were of higher yield and purity...
  2. doi request reprint HaloTag-based purification of functional human kinases from mammalian cells
    Rachel Friedman Ohana
    Promega Corporation, Madison, WI 53711, USA
    Protein Expr Purif 76:154-64. 2011
    ..In addition, when purifications using FLAG, 3xFLAG, His(6)Tag and HaloTag were performed in parallel, HaloTag was shown to provide significantly higher yields, purity and overall recovery of the expressed proteins...
  3. pmc Target engagement and drug residence time can be observed in living cells with BRET
    Matthew B Robers
    Promega Corporation, 2800 Woods Hollow Road, Fitchburg, Wisconsin 53711, USA
    Nat Commun 6:10091. 2015
    ..Our results demonstrate a novel application of BRET for assessing target engagement within the complex milieu of the intracellular environment. ..
  4. doi request reprint Deciphering the Cellular Targets of Bioactive Compounds Using a Chloroalkane Capture Tag
    Rachel Friedman Ohana
    Promega Corporation, Madison, Wisconsin, United States
    ACS Chem Biol 10:2316-24. 2015
    ..The discovery of ADO as a target may provide mechanistic insight into a reported connection between SAHA and Huntington's disease. ..
  5. doi request reprint Purification of Recombinant Proteins from Cultured Mammalian Cells by HaloTag Technology
    Rachel Friedman Ohana
    Promega Corporation, Madison, Wisconsin
    Curr Protoc Mol Biol 110:10.31.1-15. 2015
    ....
  6. doi request reprint HaloTag: a novel protein labeling technology for cell imaging and protein analysis
    Georgyi V Los
    Promega Corporation, 2800 Woods Hollow Rd, Madison, Wisconsin 53711, USA
    ACS Chem Biol 3:373-82. 2008
    ....