- Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
J Vis Exp . 2016
..In addition, use of magnetic beads allows multiple samples to be handled manually, thereby significantly improving labeling throughput. ..
- Homogeneous plate based antibody internalization assay using pH sensor fluorescent dye
Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, United States Electronic address
J Immunol Methods 431:11-21. 2016
..We finally present two new approaches using the pHAb dye, which will be beneficial for screening a large number of antibody samples during early monoclonal development phase...
- On-bead antibody-small molecule conjugation using high-capacity magnetic beads
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, United States Electronic address
J Immunol Methods 426:95-103. 2015
..This method enables simple and rapid processing of multiple samples in parallel with high-efficiency antibody recovery. It is further shown that recovered antibodies are functional and compatible with downstream applications. ..
- Improving protein array performance: focus on washing and storage conditions
Research and Development, Promega Corporation, Madison, Wisconsin 53711, USA
J Proteome Res 7:4475-82. 2008
..The findings indicate that for large ongoing studies, proteins of interest expressed in cell-free extract can be stored at -70 degrees C and repeatedly used to print small batches of protein array slides to be used over a few weeks...
- Protein-protein interaction studies on protein arrays: effect of detection strategies on signal-to-background ratios
Research and Development, Promega Corporation, Madison, WI 53711, USA
Anal Biochem 392:45-53. 2009
..This simple method for creating protein arrays in combination with our analyses of several detection strategies should increase the usefulness of protein array technologies...
- Fabrication of a reversible protein array directly from cell lysate using a stimuli-responsive polypeptide
Department of Biomedical Engineering, Duke University, Box 90281, Durham, North Carolina 27708, USA
Anal Chem 75:709-15. 2003
..TRAP has application in lab-on-a-chip bioanalytical devices as well as in the fabrication of peptide and protein arrays...
- Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches"
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708 0281, USA
J Am Chem Soc 126:7330-5. 2004
..We also investigated the molecular mechanism underlying this switch by measuring the height changes that accompany the binding and desorption steps and by adhesion force spectroscopy using atomic force microscopy...
- Label free colorimetric biosensing using nanoparticles
Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA
J Fluoresc 14:377-89. 2004
..We conclude by presenting the future prospects and challenges for the successful transition of this technology from the laboratory to a commercial product...