- Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplificationDavid S Boyle
Program for Appropriate Technology in Health, Seattle, WA, USA
MBio 4:. 2013..These pol and LTR primers amplified 98.6% and 93%, respectively, of the diverse HIV-1 variants tested. This is the first example of an isothermal assay that consistently detects all of the major HIV-1 global subtypes...
- Emerging technologies for point-of-care CD4 T-lymphocyte countingDavid S Boyle
Program for Appropriate Technology in Health PATH, Seattle, WA 98121, USA
Trends Biotechnol 30:45-54. 2012..CD4 measurement at the point-of-care (POC) in rural areas could help the facilitation of ART and monitoring of treatment. This review highlights recent technology developments with applications towards determining CD4 counts at the POC...
- Factors influencing Recombinase polymerase amplification (RPA) assay outcomes at point of careLorraine Lillis
PATH, 2201 Westlake Ave Suite 200, Seattle, WA 98121, USA Electronic address
Mol Cell Probes 30:74-8. 2016..Together our results show that reducing reaction volumes bypassed the need for the mixing step and that RPA reagents were stable even when stored for 3 weeks at very high temperatures...
- Cross-subtype detection of HIV-1 using reverse transcription and recombinase polymerase amplificationLorraine Lillis
PATH, 2201 Westlake Ave Suite 200, Seattle, WA 98121, USA
J Virol Methods 230:28-35. 2016..This data suggests that the application of RT-RPA for the combined detection of HIV-1 viral RNA and proviral DNA may prove a highly sensitive tool for rapid and accurate diagnosis of infant HIV. ..