David S Boyle

Summary

Affiliation: Program for Appropriate Technology in Health
Country: USA

Publications

  1. pmc Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplification
    David S Boyle
    Program for Appropriate Technology in Health, Seattle, WA, USA
    MBio 4:. 2013
  2. pmc Emerging technologies for point-of-care CD4 T-lymphocyte counting
    David S Boyle
    Program for Appropriate Technology in Health PATH, Seattle, WA 98121, USA
    Trends Biotechnol 30:45-54. 2012
  3. pmc Factors influencing Recombinase polymerase amplification (RPA) assay outcomes at point of care
    Lorraine Lillis
    PATH, 2201 Westlake Ave Suite 200, Seattle, WA 98121, USA Electronic address
    Mol Cell Probes 30:74-8. 2016
  4. pmc Cross-subtype detection of HIV-1 using reverse transcription and recombinase polymerase amplification
    Lorraine Lillis
    PATH, 2201 Westlake Ave Suite 200, Seattle, WA 98121, USA
    J Virol Methods 230:28-35. 2016

Collaborators

  • Lorraine Lillis
  • Jason Cantera
  • Mathew Parker
  • Dara A Lehman
  • Olaf Piepenburg
  • Julie Overbaugh
  • Joshua Siverson
  • Arthur Lee
  • Julie Weis
  • Joshua B Siverson

Detail Information

Publications4

  1. pmc Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplification
    David S Boyle
    Program for Appropriate Technology in Health, Seattle, WA, USA
    MBio 4:. 2013
    ..These pol and LTR primers amplified 98.6% and 93%, respectively, of the diverse HIV-1 variants tested. This is the first example of an isothermal assay that consistently detects all of the major HIV-1 global subtypes...
  2. pmc Emerging technologies for point-of-care CD4 T-lymphocyte counting
    David S Boyle
    Program for Appropriate Technology in Health PATH, Seattle, WA 98121, USA
    Trends Biotechnol 30:45-54. 2012
    ..CD4 measurement at the point-of-care (POC) in rural areas could help the facilitation of ART and monitoring of treatment. This review highlights recent technology developments with applications towards determining CD4 counts at the POC...
  3. pmc Factors influencing Recombinase polymerase amplification (RPA) assay outcomes at point of care
    Lorraine Lillis
    PATH, 2201 Westlake Ave Suite 200, Seattle, WA 98121, USA Electronic address
    Mol Cell Probes 30:74-8. 2016
    ..Together our results show that reducing reaction volumes bypassed the need for the mixing step and that RPA reagents were stable even when stored for 3 weeks at very high temperatures...
  4. pmc Cross-subtype detection of HIV-1 using reverse transcription and recombinase polymerase amplification
    Lorraine Lillis
    PATH, 2201 Westlake Ave Suite 200, Seattle, WA 98121, USA
    J Virol Methods 230:28-35. 2016
    ..This data suggests that the application of RT-RPA for the combined detection of HIV-1 viral RNA and proviral DNA may prove a highly sensitive tool for rapid and accurate diagnosis of infant HIV. ..