David Johnson

Summary

Affiliation: Oregon Health and Science University
Country: USA

Publications

  1. pmc Endogenous human cytomegalovirus gB is presented efficiently by MHC class II molecules to CD4+ CTL
    Nagendra R Hegde
    Department of Molecular Microbiology and Immunology, Veterans Affairs Medical Center, Portland, OR 97239, USA
    J Exp Med 202:1109-19. 2005
  2. pmc Human cytomegalovirus (HCMV) glycoprotein gB promotes virus entry in trans acting as the viral fusion protein rather than as a receptor-binding protein
    Paul T Wille
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR, USA
    MBio 4:e00332-13. 2013
  3. pmc PDGF receptor-α does not promote HCMV entry into epithelial and endothelial cells but increased quantities stimulate entry by an abnormal pathway
    Adam L Vanarsdall
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon, USA
    PLoS Pathog 8:e1002905. 2012
  4. pmc Herpes simplex virus glycoproteins gB and gD function in a redundant fashion to promote secondary envelopment
    David C Johnson
    Mail Code L 220, Dept of Microbiology and Immunology, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 85:4910-26. 2011
  5. pmc Herpes simplex virus gE/gI sorts nascent virions to epithelial cell junctions, promoting virus spread
    D C Johnson
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Virol 75:821-33. 2001
  6. pmc Directed egress of animal viruses promotes cell-to-cell spread
    David C Johnson
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Virol 76:1-8. 2002
  7. pmc Human cytomegalovirus TR strain glycoprotein O acts as a chaperone promoting gH/gL incorporation into virions but is not present in virions
    Brent J Ryckman
    Department of Molecular Microbiology and Immunology, Oregon Health and Sciences University, Portland, Oregon 97239, USA
    J Virol 84:2597-609. 2010
  8. pmc Human cytomegalovirus US2 causes similar effects on both major histocompatibility complex class I and II proteins in epithelial and glial cells
    Nagendra R Hegde
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239 3098, USA
    J Virol 77:9287-94. 2003
  9. pmc A human cytomegalovirus gO-null mutant fails to incorporate gH/gL into the virion envelope and is unable to enter fibroblasts and epithelial and endothelial cells
    Paul T Wille
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Virol 84:2585-96. 2010
  10. pmc Human cytomegalovirus US7, US8, US9, and US10 are cytoplasmic glycoproteins, not found at cell surfaces, and US9 does not mediate cell-to-cell spread
    Mary T Huber
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Virol 76:5748-58. 2002

Research Grants

Collaborators

  • Brent J Ryckman
  • M A Jarvis
  • Aleksandra Snyder
  • DAVID LEWINSOHN
  • Richard J Roller
  • Yasushi Kawaguchi
  • J D Baines
  • BARRY ROUSE
  • N Jan Chalupny
  • PATRICIA SPEAR
  • Todd W Wisner
  • Aaron Farnsworth
  • Adam L Vanarsdall
  • Nagendra R Hegde
  • Paul T Wille
  • Mathieu S Chevalier
  • Katarina Polcicova
  • Catherine C Wright
  • Claire Dunn
  • Marie C Chase
  • Jay A Nelson
  • Derek D Sloan
  • Wendy J Collins
  • Mary T Huber
  • Hetian Lei
  • Andrius Kazlauskas
  • Ken Sugimoto
  • Paul W Howard
  • Amber J Knoche
  • Gary H Cohen
  • Roselyn J Eisenberg
  • Fan Mou
  • Brian P Hannah
  • Akihisa Kato
  • Michael Webb
  • Gary Cohen
  • Roselyn Eisenberg
  • Miri Yoon
  • Keith R Jerome
  • Lori Frappier
  • Aaron J Hinz
  • Jin Young Han
  • Kathy Shire
  • Michael C Denton
  • Mary Stewart
  • Tracy K Sandifer
  • Kaustuv Banerjee
  • Partha Sarathi Biswas
  • Barb L Rainish
  • Kim Goldsmith
  • P V Sivakumar
  • David Cosman
  • Stephanie Dosch
  • Kimberly Goldsmith
  • Claire L Sutherland
  • Roman Tomazin
  • Jessica M Boname
  • Todd Wisner
  • Jessica Boname
  • Roman A Tomazin
  • Gwynn M Daniels

Detail Information

Publications35

  1. pmc Endogenous human cytomegalovirus gB is presented efficiently by MHC class II molecules to CD4+ CTL
    Nagendra R Hegde
    Department of Molecular Microbiology and Immunology, Veterans Affairs Medical Center, Portland, OR 97239, USA
    J Exp Med 202:1109-19. 2005
    ....
  2. pmc Human cytomegalovirus (HCMV) glycoprotein gB promotes virus entry in trans acting as the viral fusion protein rather than as a receptor-binding protein
    Paul T Wille
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR, USA
    MBio 4:e00332-13. 2013
    ..In contrast, virus particles lacking gH/gL could not enter cells expressing gH/gL. Our study supports the hypothesis that gB is the fusion protein and gH/gL acts upstream of gB to bind receptors and then activate gB for fusion...
  3. pmc PDGF receptor-α does not promote HCMV entry into epithelial and endothelial cells but increased quantities stimulate entry by an abnormal pathway
    Adam L Vanarsdall
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon, USA
    PLoS Pathog 8:e1002905. 2012
    ..Given that PDGFRα increased infection of some cells to 90%, PDGFRα may be very useful in overcoming inefficient HCMV entry (even of lab strains) into the many difficult-to-infect cell types...
  4. pmc Herpes simplex virus glycoproteins gB and gD function in a redundant fashion to promote secondary envelopment
    David C Johnson
    Mail Code L 220, Dept of Microbiology and Immunology, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 85:4910-26. 2011
    ..Our results suggest that HSV gB functions in secondary envelopment, apparently acting downstream of gE/gI...
  5. pmc Herpes simplex virus gE/gI sorts nascent virions to epithelial cell junctions, promoting virus spread
    D C Johnson
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Virol 75:821-33. 2001
    ..Delivery of virus particles to cell junctions would be expected to enhance virus spread and enable viruses to avoid host immune defenses...
  6. pmc Directed egress of animal viruses promotes cell-to-cell spread
    David C Johnson
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Virol 76:1-8. 2002
  7. pmc Human cytomegalovirus TR strain glycoprotein O acts as a chaperone promoting gH/gL incorporation into virions but is not present in virions
    Brent J Ryckman
    Department of Molecular Microbiology and Immunology, Oregon Health and Sciences University, Portland, Oregon 97239, USA
    J Virol 84:2597-609. 2010
    ..Thus, our revised model suggests that both gH/gL and gH/gL/UL128-131 are required for entry into epithelial and endothelial cells...
  8. pmc Human cytomegalovirus US2 causes similar effects on both major histocompatibility complex class I and II proteins in epithelial and glial cells
    Nagendra R Hegde
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239 3098, USA
    J Virol 77:9287-94. 2003
    ..We concluded that US2 has broad effects in a variety of cells that express both class I and II proteins and is relevant to HCMV infection in vivo...
  9. pmc A human cytomegalovirus gO-null mutant fails to incorporate gH/gL into the virion envelope and is unable to enter fibroblasts and epithelial and endothelial cells
    Paul T Wille
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Virol 84:2585-96. 2010
    ....
  10. pmc Human cytomegalovirus US7, US8, US9, and US10 are cytoplasmic glycoproteins, not found at cell surfaces, and US9 does not mediate cell-to-cell spread
    Mary T Huber
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Virol 76:5748-58. 2002
    ..Together, our results do not support the hypothesis that US9 plays a direct role in HCMV cell-to-cell spread...
  11. pmc Herpes simplex virus gE/gI and US9 proteins promote transport of both capsids and virion glycoproteins in neuronal axons
    Aleksandra Snyder
    Department of Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Virol 82:10613-24. 2008
    ..Our observations suggest that gE/gI and US9 function in the neuron cell body to promote the loading of capsids and glycoprotein-containing vesicles onto microtubule motors that ferry HSV structural components toward axon tips...
  12. pmc A herpes simplex virus gD-YFP fusion glycoprotein is transported separately from viral capsids in neuronal axons
    Aleksandra Snyder
    Department of Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 81:8337-40. 2007
    ..Anti-gD antibodies colocalized with gD-YFP, indicating that gD-YFP behaves like wild-type HSV gD...
  13. pmc Characterization of the human cytomegalovirus gH/gL/UL128-131 complex that mediates entry into epithelial and endothelial cells
    Brent J Ryckman
    6366 Basic Sciences Building, Mail Code L 220, Department of Molecular Microbiology and Immunology, Oregon Health and Sciences University, 3181 Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 82:60-70. 2008
    ..Thus, we concluded that UL128, UL130, and UL131 must all bind simultaneously onto gH/gL for the production of complexes that can function in entry into epithelial and endothelial cells...
  14. pmc HCMV gH/gL/UL128-131 interferes with virus entry into epithelial cells: evidence for cell type-specific receptors
    Brent J Ryckman
    Department of Molecular Microbiology and Immunology, Oregon Health and Sciences University, Portland, OR 97239, USA
    Proc Natl Acad Sci U S A 105:14118-23. 2008
    ..We concluded that epithelial cells express gH/gL/UL128-131 receptors that mediate HCMV entry. Fibroblasts either lack the gH/gL/UL128-131 receptors, the receptors are more numerous, or fibroblasts express other functional receptors...
  15. pmc Herpes simplex virus gE/gI expressed in epithelial cells interferes with cell-to-cell spread
    Wendy J Collins
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, OR 97239, USA
    J Virol 77:2686-95. 2003
    ..Therefore, like gD, gE/gI appears to be able to interact with cellular components of cell junctions, gE/gI receptors which can promote HSV cell-to-cell spread...
  16. pmc Binding of human cytomegalovirus US2 to major histocompatibility complex class I and II proteins is not sufficient for their degradation
    Mathieu S Chevalier
    Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201, USA
    J Virol 76:8265-75. 2002
    ..Our results are consistent with the hypothesis that US2 couples MHC proteins to components of the ER degradation pathway, enormously increasing the rate of degradation of MHC proteins...
  17. pmc Inhibition of HLA-DR assembly, transport, and loading by human cytomegalovirus glycoprotein US3: a novel mechanism for evading major histocompatibility complex class II antigen presentation
    Nagendra R Hegde
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Virol 76:10929-41. 2002
    ..We concluded that US3 and US2 can collaborate to inhibit class II-mediated presentation of endogenous HCMV antigens to CD4(+) T cells, allowing virus-infected cells to resist recognition by CD4(+) T cells...
  18. pmc Human cytomegalovirus US3 chimeras containing US2 cytosolic residues acquire major histocompatibility class I and II protein degradation properties
    Mathieu S Chevalier
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Virol 77:4731-8. 2003
    ..Therefore, it appears that the CT domain of US2 participates in recruiting p97 ATPase into ER-associated degradation complexes...
  19. pmc Herpes simplex virus capsids are transported in neuronal axons without an envelope containing the viral glycoproteins
    Aleksandra Snyder
    Dept of Mol Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 80:11165-77. 2006
    ..We concluded that HSV capsids are transported in axons without an envelope containing viral glycoproteins, with glycoproteins transported separately and assembling with capsids at axon termini...
  20. pmc Human cytomegalovirus glycoprotein UL16 causes intracellular sequestration of NKG2D ligands, protecting against natural killer cell cytotoxicity
    Claire Dunn
    Department of Molecular Microbiology and Immunology, Oregon Health and Sciences University, Portland, OR 97239, USA
    J Exp Med 197:1427-39. 2003
    ..The intracellular sequestration of NKG2D ligands by UL16 represents a novel HCMV immune evasion mechanism to add to the well-documented viral strategies directed against antigen presentation by classical MHC molecules...
  21. pmc Human cytomegalovirus entry into epithelial and endothelial cells depends on genes UL128 to UL150 and occurs by endocytosis and low-pH fusion
    Brent J Ryckman
    Department of Molecular Microbiology and Immunology, Oregon Health and Sciences University, 3181 Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 80:710-22. 2006
    ..Together, these data indicate that genes UL128 to UL150 are required for HCMV adsorption and penetration of epithelial cells and to promote some early stage of virus replication, subsequent to virus entry, in endothelial cells...
  22. pmc Herpes simplex virus glycoproteins gD and gE/gI serve essential but redundant functions during acquisition of the virion envelope in the cytoplasm
    Aaron Farnsworth
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Virol 77:8481-94. 2003
    ..In the absence of either one of these HSV glycoproteins, envelopment proceeds; however, without both gD and gE, or gE/gI, there is profound inhibition of cytoplasmic envelopment...
  23. ncbi request reprint The role of BiP in endoplasmic reticulum-associated degradation of major histocompatibility complex class I heavy chain induced by cytomegalovirus proteins
    Nagendra R Hegde
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, 97239, USA
    J Biol Chem 281:20910-9. 2006
    ..These data were consistent with a model in which US2 and US11 bridge HC onto BiP promoting interactions with other ER-associated degradation proteins...
  24. pmc Herpesvirus gB-induced fusion between the virion envelope and outer nuclear membrane during virus egress is regulated by the viral US3 kinase
    Todd W Wisner
    Department of Molecular Microbiology and Immunology, Oregon Health and Sciences University, Portland, Oregon 97239, USA
    J Virol 83:3115-26. 2009
    ..By this packaging, US3 might be brought close to the gB CT tail, leading to phosphorylation and triggering fusion between the virion envelope and the outer NM...
  25. pmc Human cytomegalovirus glycoproteins gB and gH/gL mediate epithelial cell-cell fusion when expressed either in cis or in trans
    Adam L Vanarsdall
    Dept of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 82:11837-50. 2008
    ..Expression of gB and gH/gL in trans with different cell types revealed surface molecules that are required for fusion on HCMV-permissive cells but not on nonpermissive cells...
  26. pmc Herpes keratitis in the absence of anterograde transport of virus from sensory ganglia to the cornea
    Katarina Polcicova
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    Proc Natl Acad Sci U S A 102:11462-7. 2005
    ..Nevertheless, US9- HSV caused keratitis. Therefore, herpes keratitis can occur without anterograde transport from ganglia to the cornea, probably mediated by virus persistent in the cornea...
  27. pmc Herpes simplex virus gE/gI must accumulate in the trans-Golgi network at early times and then redistribute to cell junctions to promote cell-cell spread
    Aaron Farnsworth
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 80:3167-79. 2006
    ..Additionally, gE CT sequences between residues 495 and 519, which contain no obvious cell sorting motifs, are required to promote gE/gI traffic to cell junctions and cell-to-cell spread...
  28. pmc Redistribution of cellular and herpes simplex virus proteins from the trans-golgi network to cell junctions without enveloped capsids
    Todd W Wisner
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Mail Code L 220, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 78:11519-35. 2004
    ..This is consistent with the hypothesis that there are late HSV proteins that reorganize or redistribute TGN/endosomal compartments to promote virus egress and cell-to-cell spread...
  29. pmc Fusion between perinuclear virions and the outer nuclear membrane requires the fusogenic activity of herpes simplex virus gB
    Catherine C Wright
    Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    J Virol 83:11847-56. 2009
    ..These results support the hypothesis that gB functions directly to mediate the fusion between perinuclear virus particles and the outer NM...
  30. pmc The extracellular domain of herpes simplex virus gE is indispensable for efficient cell-to-cell spread: evidence for gE/gI receptors
    Katarina Polcicova
    L 220, Room 6366 BSc, Department of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 79:11990-2001. 2005
    ..This fits with similarities in structure and function of gE/gI and gD, which is a receptor binding protein...
  31. pmc Human cytomegalovirus glycoprotein gO complexes with gH/gL, promoting interference with viral entry into human fibroblasts but not entry into epithelial cells
    Adam L Vanarsdall
    L 220, Department of Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 85:11638-45. 2011
    ..Together, the results provide additional support for our hypotheses that epithelial cells express putative gH/gL/UL128-1331 receptors important for HCMV entry and that fibroblasts express distinct gH/gL receptors...
  32. pmc Anterograde transport of herpes simplex virus capsids in neurons by both separate and married mechanisms
    Todd W Wisner
    Department of Microbiology and Immunology, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 85:5919-28. 2011
    ..Together, our results suggest that one can observe anterograde transport of both HSV capsids and enveloped virus particles depending on which neurons are cultured and how the neurons are imaged...
  33. pmc Herpes simplex virus glycoproteins gB and gH function in fusion between the virion envelope and the outer nuclear membrane
    Aaron Farnsworth
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    Proc Natl Acad Sci U S A 104:10187-92. 2007
    ..It is noteworthy that fusion associated with HSV entry requires the cooperative action of both gB and gH, suggesting that the two types of fusion (egress versus entry) are dissimilar processes...
  34. pmc Cytoplasmic residues of herpes simplex virus glycoprotein gE required for secondary envelopment and binding of tegument proteins VP22 and UL11 to gE and gD
    Aaron Farnsworth
    Dept of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    J Virol 81:319-31. 2007
    ..Nevertheless, assays involving TAP proteins and viral proteins expressed by HSV and Ad vectors supported the conclusion that VP22 and UL11 interact specifically with the CT domains of gD and gE...
  35. ncbi request reprint Inhibition of TCR signaling by herpes simplex virus
    Derek D Sloan
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
    J Immunol 176:1825-33. 2006
    ..Our findings elucidate a potentially novel method of viral immune evasion that could be exploited to better manage HSV infection, aid in vaccine design, or allow targeted manipulation of T cell function...

Research Grants3

  1. Hantavirus Vaccines Based On Nonreplicating Adenoviruses
    David Johnson; Fiscal Year: 2005
    ..This will be a prelude to challenge experiments in which vaccinated hamsters will be challenged with Andes virus that causes a lethal pulmonary disease similar to that in humans. ..
  2. Herpes Simplex Virus Transmission Across Cell Junctions
    David Johnson; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  3. HCMV US2 & US11 Inhibition of MHC Class II Presentation
    David Johnson; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..