Tomohiko Sugiyama

Summary

Affiliation: Ohio University
Country: USA

Publications

  1. pmc Rad52-mediated DNA annealing after Rad51-mediated DNA strand exchange promotes second ssDNA capture
    Tomohiko Sugiyama
    Department of Biological Sciences, Ohio University, Athens, OH 45701, USA
    EMBO J 25:5539-48. 2006
  2. doi request reprint Dynamic regulatory interactions of rad51, rad52, and replication protein-a in recombination intermediates
    Tomohiko Sugiyama
    Department of Biological Sciences, Ohio University, Athens, 45701, USA
    J Mol Biol 390:45-55. 2009
  3. ncbi request reprint Rad52 protein associates with replication protein A (RPA)-single-stranded DNA to accelerate Rad51-mediated displacement of RPA and presynaptic complex formation
    Tomohiko Sugiyama
    Section of Microbiology and Center for Genetics and Development, University of California, Davis, California 95616 8665, USA
    J Biol Chem 277:31663-72. 2002
  4. pmc Escherichia coli RecO protein anneals ssDNA complexed with its cognate ssDNA-binding protein: A common step in genetic recombination
    Noriko Kantake
    Sections of Microbiology and Molecular and Cellular Biology, Genetics Graduate Group, University of California, Davis 95616, USA
    Proc Natl Acad Sci U S A 99:15327-32. 2002
  5. ncbi request reprint The recombination-deficient mutant RPA (rfa1-t11) is displaced slowly from single-stranded DNA by Rad51 protein
    Noriko Kantake
    Sections of Microbiology and of Molecular and Cellular Biology, Center for Genetics and Development, University of California, Davis, California 95616, USA
    J Biol Chem 278:23410-7. 2003
  6. ncbi request reprint DNA annealing mediated by Rad52 and Rad59 proteins
    Yun Wu
    Section of Microbiology, Center for Genetics and Development, Division of Biological Sciences, University of California, Davis, California 95616 8665, USA
    J Biol Chem 281:15441-9. 2006
  7. ncbi request reprint The DNA binding preference of RAD52 and RAD59 proteins: implications for RAD52 and RAD59 protein function in homologous recombination
    Yun Wu
    Section of Microbiology, Center for Genetics and Development, University of California, Davis, California 95616 8665, USA
    J Biol Chem 281:40001-9. 2006
  8. pmc Rad51 protein controls Rad52-mediated DNA annealing
    Yun Wu
    Sections of Microbiology and of Molecular and Cellular Biology, University of California, Davis, CA 95616 8665, USA
    J Biol Chem 283:14883-92. 2008

Collaborators

  • Yun Wu
  • Stephen C Kowalczykowski
  • Noriko Kantake
  • Joseph S Siino
  • Richard D Kolodner
  • Murty V V M Madiraju

Detail Information

Publications8

  1. pmc Rad52-mediated DNA annealing after Rad51-mediated DNA strand exchange promotes second ssDNA capture
    Tomohiko Sugiyama
    Department of Biological Sciences, Ohio University, Athens, OH 45701, USA
    EMBO J 25:5539-48. 2006
    ..We also demonstrate that the two activities of Rad52, recruiting Rad51 and annealing DNA, are coordinated in DNA strand exchange and second ssDNA capture...
  2. doi request reprint Dynamic regulatory interactions of rad51, rad52, and replication protein-a in recombination intermediates
    Tomohiko Sugiyama
    Department of Biological Sciences, Ohio University, Athens, 45701, USA
    J Mol Biol 390:45-55. 2009
    ..This suggests an additional regulatory role for Rad51 following DNA strand invasion, where Rad51-double-stranded DNA may inhibit illegitimate second-end capture to ensure the error-free repair of a DNA double-strand break...
  3. ncbi request reprint Rad52 protein associates with replication protein A (RPA)-single-stranded DNA to accelerate Rad51-mediated displacement of RPA and presynaptic complex formation
    Tomohiko Sugiyama
    Section of Microbiology and Center for Genetics and Development, University of California, Davis, California 95616 8665, USA
    J Biol Chem 277:31663-72. 2002
    ..This acceleration probably involves direct interactions with both Rad51 protein and RPA. Detection of a Rad52-RPA-ssDNA co-complex suggests that this co-complex is an intermediate in the displacement process...
  4. pmc Escherichia coli RecO protein anneals ssDNA complexed with its cognate ssDNA-binding protein: A common step in genetic recombination
    Noriko Kantake
    Sections of Microbiology and Molecular and Cellular Biology, Genetics Graduate Group, University of California, Davis 95616, USA
    Proc Natl Acad Sci U S A 99:15327-32. 2002
    ..Based on the conservation of these functions, we propose a modified double-strand break repair model that includes DNA annealing as an important intermediate step...
  5. ncbi request reprint The recombination-deficient mutant RPA (rfa1-t11) is displaced slowly from single-stranded DNA by Rad51 protein
    Noriko Kantake
    Sections of Microbiology and of Molecular and Cellular Biology, Center for Genetics and Development, University of California, Davis, California 95616, USA
    J Biol Chem 278:23410-7. 2003
    ..These in vitro results suggest that, in vivo, RPA is bound to ssDNA prior to Rad51 protein and that RPA displacement by Rad51 protein is a critical step in homologous recombination, which is impaired in the rfa1-t11 mutation...
  6. ncbi request reprint DNA annealing mediated by Rad52 and Rad59 proteins
    Yun Wu
    Section of Microbiology, Center for Genetics and Development, Division of Biological Sciences, University of California, Davis, California 95616 8665, USA
    J Biol Chem 281:15441-9. 2006
    ..These results suggest that Rad59 performs different functions in the recombination process, and should be more accurately viewed as a Rad52 paralogue...
  7. ncbi request reprint The DNA binding preference of RAD52 and RAD59 proteins: implications for RAD52 and RAD59 protein function in homologous recombination
    Yun Wu
    Section of Microbiology, Center for Genetics and Development, University of California, Davis, California 95616 8665, USA
    J Biol Chem 281:40001-9. 2006
    ..cerevisiae Rad52 and Rad59 proteins and their eukaryotic counterparts function by binding to single-stranded DNA formed as intermediates of recombination rather than by binding to the unprocessed DNA double-strand break...
  8. pmc Rad51 protein controls Rad52-mediated DNA annealing
    Yun Wu
    Sections of Microbiology and of Molecular and Cellular Biology, University of California, Davis, CA 95616 8665, USA
    J Biol Chem 283:14883-92. 2008
    ..This channeling determines the nature of the subsequent repair process and is consistent with the observed competition between these pathways in vivo...