Jing Zheng

Summary

Affiliation: Northwestern University
Country: USA

Publications

  1. ncbi request reprint N-linked glycosylation sites of the motor protein prestin: effects on membrane targeting and electrophysiological function
    Keiji Matsuda
    Auditory Physiology Laboratory, Department of Communication Sciences and Disorders, Northwestern University, Evanston, Illinois 60208, USA
    J Neurochem 89:928-38. 2004
  2. ncbi request reprint The C-terminus of prestin influences nonlinear capacitance and plasma membrane targeting
    Jing Zheng
    Auditory Physiology Laboratory, Department of Communication Sciences and Disorders, Northwestern University, Evanston, IL 60208 USA
    J Cell Sci 118:2987-96. 2005
  3. ncbi request reprint Prestin, the motor protein of outer hair cells
    Jing Zheng
    Department of Communication Sciences and Disorders, Auditory Physiology Laboratory, The Hugh Knowles Center, Northwestern University, Evanston, Ill 60208, USA
    Audiol Neurootol 7:9-12. 2002
  4. ncbi request reprint Analysis of the oligomeric structure of the motor protein prestin
    Jing Zheng
    Department of Communication Sciences and Disorders, Northwestern University, Evanston, Illinois 60208, USA
    J Biol Chem 281:19916-24. 2006
  5. pmc Identifying components of the hair-cell interactome involved in cochlear amplification
    Jing Zheng
    Department of Communication Sciences and Disorders, The Hugh Knowles Center, Northwestern University, Evanston, IL 60208, USA
    BMC Genomics 10:127. 2009
  6. ncbi request reprint Genomic characterization and expression of mouse prestin, the motor protein of outer hair cells
    Jing Zheng
    Auditory Physiology Laboratory The Hugh Knowles Center, Department of Communication Sciences and Disorders, Northwestern University, Frances Searle Building, 2240 Campus Drive, Evanston, Illinois 60208, USA
    Mamm Genome 14:87-96. 2003
  7. ncbi request reprint Identification of differentially expressed cDNA clones from gerbil cochlear outer hair cells
    Jing Zheng
    Auditory Physiology Laboratory Hugh Knowles Center, Department of Communication Sciences and Disorders, Northwestern University, Evanston, IL 60802, USA
    Audiol Neurootol 7:277-88. 2002
  8. pmc Interaction between CFTR and prestin (SLC26A5)
    Kazuaki Homma
    Department of Communication Sciences and Disorders, The Hugh Knowles Center, Northwestern University, Evanston, IL 60208, USA
    Biochim Biophys Acta 1798:1029-40. 2010
  9. pmc EHD4 and CDH23 are interacting partners in cochlear hair cells
    Soma Sengupta
    Department of Communication Sciences and Disorders, Hugh Knowles Center, Northwestern University, Evanston, Illinois 60208, USA
    J Biol Chem 284:20121-9. 2009
  10. pmc A chimera analysis of prestin knock-out mice
    Mary Ann Cheatham
    Departments of Communication Sciences and Disorders, The Hugh Knowles Center, Northwestern University, Evanston, Illinois 60208, USA
    J Neurosci 29:12000-8. 2009

Research Grants

Collaborators

Detail Information

Publications43

  1. ncbi request reprint N-linked glycosylation sites of the motor protein prestin: effects on membrane targeting and electrophysiological function
    Keiji Matsuda
    Auditory Physiology Laboratory, Department of Communication Sciences and Disorders, Northwestern University, Evanston, Illinois 60208, USA
    J Neurochem 89:928-38. 2004
    ..These data suggest that glycosylation of prestin may quantitatively affect OHC electromotility...
  2. ncbi request reprint The C-terminus of prestin influences nonlinear capacitance and plasma membrane targeting
    Jing Zheng
    Auditory Physiology Laboratory, Department of Communication Sciences and Disorders, Northwestern University, Evanston, IL 60208 USA
    J Cell Sci 118:2987-96. 2005
    ..Together, these data suggest that certain specific sequences and individual amino acids in the C-terminus are necessary for correct cellular distribution and function...
  3. ncbi request reprint Prestin, the motor protein of outer hair cells
    Jing Zheng
    Department of Communication Sciences and Disorders, Auditory Physiology Laboratory, The Hugh Knowles Center, Northwestern University, Evanston, Ill 60208, USA
    Audiol Neurootol 7:9-12. 2002
    ..Prestin shares overall structure similarity with this anion transporter family. Recently, intracellular anions (chloride or bicarbonate) were found to be essential for OHC electromotility and prestin's function...
  4. ncbi request reprint Analysis of the oligomeric structure of the motor protein prestin
    Jing Zheng
    Department of Communication Sciences and Disorders, Northwestern University, Evanston, Illinois 60208, USA
    J Biol Chem 281:19916-24. 2006
    ..This stable dimer may act as the building block for producing the higher order oligomers that form the approximately 11-nM particles in the outer hair cell's basolateral membrane...
  5. pmc Identifying components of the hair-cell interactome involved in cochlear amplification
    Jing Zheng
    Department of Communication Sciences and Disorders, The Hugh Knowles Center, Northwestern University, Evanston, IL 60208, USA
    BMC Genomics 10:127. 2009
    ..Prestin is a basolateral membrane protein responsible for OHC somatic electromotility...
  6. ncbi request reprint Genomic characterization and expression of mouse prestin, the motor protein of outer hair cells
    Jing Zheng
    Auditory Physiology Laboratory The Hugh Knowles Center, Department of Communication Sciences and Disorders, Northwestern University, Frances Searle Building, 2240 Campus Drive, Evanston, Illinois 60208, USA
    Mamm Genome 14:87-96. 2003
    ..Sequence analysis of the ~500 bp upstream from exon 1 revealed multiple potential transcription factor binding sites, including both TATA and GC boxes, as well as other regulatory-element binding sites...
  7. ncbi request reprint Identification of differentially expressed cDNA clones from gerbil cochlear outer hair cells
    Jing Zheng
    Auditory Physiology Laboratory Hugh Knowles Center, Department of Communication Sciences and Disorders, Northwestern University, Evanston, IL 60802, USA
    Audiol Neurootol 7:277-88. 2002
    ....
  8. pmc Interaction between CFTR and prestin (SLC26A5)
    Kazuaki Homma
    Department of Communication Sciences and Disorders, The Hugh Knowles Center, Northwestern University, Evanston, IL 60208, USA
    Biochim Biophys Acta 1798:1029-40. 2010
    ..Electrophysiological experiments demonstrated that cAMP-activated CFTR is capable of enhancing voltage-dependent charge displacement, a signature of OHC motility, whereas prestin does not affect the chloride conductance of CFTR...
  9. pmc EHD4 and CDH23 are interacting partners in cochlear hair cells
    Soma Sengupta
    Department of Communication Sciences and Disorders, Hugh Knowles Center, Northwestern University, Evanston, Illinois 60208, USA
    J Biol Chem 284:20121-9. 2009
    ..Taken together, these data indicate that EHD4 is a novel CDH23-interacting protein that could regulate CDH23 trafficking/localization in a calcium-sensitive manner...
  10. pmc A chimera analysis of prestin knock-out mice
    Mary Ann Cheatham
    Departments of Communication Sciences and Disorders, The Hugh Knowles Center, Northwestern University, Evanston, Illinois 60208, USA
    J Neurosci 29:12000-8. 2009
    ..Accordingly, we demonstrate a proportional reduction in sensitivity and in the tip length of CAP tuning curves as the number of OHCs derived from the KO genome increases; i.e., genotype ratio and phenotype are closely related...
  11. pmc Prestin-based outer hair cell motility is necessary for mammalian cochlear amplification
    Peter Dallos
    Department of Neurobiology and Physiology, Northwestern University, Evanston, IL 60208, USA
    Neuron 58:333-9. 2008
    ..These animals have knockout-like behavior, demonstrating that prestin-based electromotility is required for cochlear amplification...
  12. pmc Prestin and the cochlear amplifier
    Peter Dallos
    Department of Neurobiology and Physiology, Northwestern University, 2240 Campus Drive, Evanston, IL 60208, USA
    J Physiol 576:37-42. 2006
    ..Here we consider new work pertaining to this protein and its potential role as the mammalian cochlear amplifier...
  13. pmc Effects of cyclic nucleotides on the function of prestin
    Levente Deak
    Auditory Physiology Laboratory, Department of Communication Sciences and Disorders, The Neuroscience Institute, Northwestern University, Evanston, IL 60208, USA
    J Physiol 563:483-96. 2005
    ..One of these sites may influence the membrane targeting process of prestin. Finally, a new topology map of prestin is proposed...
  14. pmc Interaction between the motor protein prestin and the transporter protein VAPA
    Soma Sengupta
    Department of Otolaryngology, Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Chicago, IL 60611, USA
    Biochim Biophys Acta 1803:796-804. 2010
    ..These data suggest that VAPA could be involved in prestin's transportation inside OHCs and may facilitate the targeting of this abundant OHC protein to the plasma membrane...
  15. pmc Functional regulation of the SLC26-family protein prestin by calcium/calmodulin
    Jacob Pearson Keller
    Departments of Communication Sciences and Disorders, Molecular Biosciences, Neurobiology, and Otolaryngology Head and Neck Surgery, and The Hugh Knowles Center for Clinical and Basic Science in Hearing and Its Disorders, Northwestern University, Evanston, Illinois 60208
    J Neurosci 34:1325-32. 2014
    ..Hence, the calcium/CaM-dependent regulatory mechanism described herein is likely applicable to most, if not all, SLC26 paralogs. ..
  16. pmc Marshalin, a microtubule minus-end binding protein, regulates cytoskeletal structure in the organ of Corti
    Jing Zheng
    Department of Otolaryngology Head and Neck Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA Hugh Knowles Center for Clinical and Basic Science in Hearing and Its Disorders, Northwestern University, Evanston, IL 60208, USA
    Biol Open 2:1192-202. 2013
    ..As a scaffolding protein with multiple isoforms, marshalin is capable of modifying cytoskeletal networks, and consequently organelle positioning, through interactions with various protein partners present in different cells. ..
  17. pmc The V499G/Y501H mutation impairs fast motor kinetics of prestin and has significance for defining functional independence of individual prestin subunits
    Kazuaki Homma
    Department of Otolaryngology Head and Neck Surgery, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Biol Chem 288:2452-63. 2013
    ..These results suggest that prestin subunits are individually functional within a given multimer...
  18. pmc Isolation of outer hair cells from the cochlear sensory epithelium in whole-mount preparation using laser capture microdissection
    Charles T Anderson
    Department of Communication Sciences and Disorders, Northwestern University, Frances Searle Building, 2240 Campus Drive, Evanston, IL 60208, USA
    J Neurosci Methods 162:229-36. 2007
    ..A similar approach can also be used to isolate large quantities of inner hair cells and selected supporting cells from the whole-mount cochlear preparation...
  19. pmc Prestin-Dependence of Outer Hair Cell Survival and Partial Rescue of Outer Hair Cell Loss in PrestinV499G/Y501H Knockin Mice
    Mary Ann Cheatham
    The Knowles Hearing Center, Roxelyn and Richard Pepper Department of Communication Sciences and Disorders, Northwestern University, Evanston, IL, 60208, United States of America
    PLoS ONE 10:e0145428. 2015
    ..We also report that improved OHC survival in 499 prestin KIs had little effect on hearing phenotype, reaffirming the original contention about the essential role of prestin's motor function in cochlear amplification. ..
  20. pmc Cadherin 23-C Regulates Microtubule Networks by Modifying CAMSAP3's Function
    Satoe Takahashi
    Department of Otolaryngology Head and Neck Surgery, Feinberg School of Medicine, Northwestern University, Chicago IL 60611, USA
    Sci Rep 6:28706. 2016
    ..Together, our data suggest that CDH23-C is a CAMSAP3/Marshalin-binding protein that can modify MT networks indirectly through its interaction with CAMSAP3/Marshalin. ..
  21. pmc The R130S mutation significantly affects the function of prestin, the outer hair cell motor protein
    Satoe Takahashi
    Department of Otolaryngology Head and Neck Surgery, Feinberg School of Medicine, Northwestern University, 303 E Chicago Ave, Chicago, IL, 60611, USA
    J Mol Med (Berl) 94:1053-62. 2016
    ..Our study thus provides experimental evidence that supports a causal relationship between the R130S mutation in the prestin gene and hearing loss found in patients with this missense mutation...
  22. pmc Susceptibility of outer hair cells to cholesterol chelator 2-hydroxypropyl-β-cyclodextrine is prestin-dependent
    Satoe Takahashi
    Department of Otolaryngology Head and Neck Surgery, Feinberg School of Medicine, Northwestern University, Chicago IL 60611, USA
    Sci Rep 6:21973. 2016
    ..These results suggest that a prestin-dependent mechanism contributes to HPβCD ototoxicity. ..
  23. ncbi request reprint Prestin expression in the cochlea of the reeler mouse
    Jing Zheng
    Department of Communication Sciences and Disorders, 2240 Campus Drive, Northwestern University, Evanston, IL 60208 3650, USA
    Neurosci Lett 347:13-6. 2003
    ..Because prestin expression is not affected in reeler mice, prestin's coding region, as well as any regulatory elements, is predicted to lie in the 17 kb that separate prestin's exon 1 from the end of the deletion...
  24. pmc Carcinoembryonic antigen-related cell adhesion molecule 16 interacts with alpha-tectorin and is mutated in autosomal dominant hearing loss (DFNA4)
    Jing Zheng
    Department of Otolaryngology Head and Neck Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 108:4218-23. 2011
    ..In aggregate, these data identify CEACAM16 as an α-tectorin-interacting protein that concentrates at the point of attachment of the TM to the stereocilia and, when mutated, results in ADNSHL at the DFNA4 locus...
  25. pmc Linking neurogenetics and individual differences in language learning: the dopamine hypothesis
    Patrick C M Wong
    Roxelyn and Richard Pepper Department of Communication Sciences and Disorders, Northwestern University, Evanston, IL, USA
    Cortex 48:1091-102. 2012
    ..If this hypothesis is supported by future empirical findings we suggest that it may point to neurogenetic markers that allow for language learning to be personalized...
  26. pmc The derived allele of ASPM is associated with lexical tone perception
    Patrick C M Wong
    Roxelyn and Richard Pepper Department of Communication Sciences and Disorders, Northwestern University, Evanston, Illinois, United States of America
    PLoS ONE 7:e34243. 2012
    ..These results suggest that the association between the recently derived allele of ASPM is likely to be specific and is tied to higher level brain functions in the temporal cortex related to human communication...
  27. ncbi request reprint Prestin, a cochlear motor protein, is defective in non-syndromic hearing loss
    Xue Zhong Liu
    Department of Otolaryngology, University of Miami, Miami, FL 33101, USA
    Hum Mol Genet 12:1155-62. 2003
    ..Finally, the observation of this mutation only in the Caucasian probands indicated an association with a specific ethnic background. This study thereby reveals an essential function of prestin in human auditory processing...
  28. ncbi request reprint A new mutation in the human pres gene and its effect on prestin function
    Timea Toth
    Medical and Health Science Center, Department of Otolaryngology, University of Debrecen, Debrecen, Hungary
    Int J Mol Med 20:545-50. 2007
    ....
  29. pmc Glucose transporter 5 is undetectable in outer hair cells and does not contribute to cochlear amplification
    Xudong Wu
    Department of Developmental Neurobiology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Brain Res 1210:20-8. 2008
    ..e., nonlinear capacitance and electromotility) and normal cochlear sensitivity and frequency selectivity. We conclude that Glut5 is not required for OHC motility or cochlear amplification...
  30. pmc Prestin-based outer hair cell electromotility in knockin mice does not appear to adjust the operating point of a cilia-based amplifier
    Jiangang Gao
    Department of Developmental Neurobiology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 104:12542-7. 2007
    ..However, they are consistent with the notion that the AC component of OHC somatic motility plays a dominant role in mammalian cochlear amplification...
  31. ncbi request reprint Expression of a releasable form of annexin II by human keratinocytes
    Feridoun Karimi-Busheri
    Experimental Oncology, Cross Cancer Institute, Canada
    J Cell Biochem 86:737-47. 2002
    ..This finding is consistent with the localization of annexin II detected by immunohistochemical staining...
  32. ncbi request reprint Co-expression of vascular endothelial growth factor and neuropilin-1 in ovine feto-placental artery endothelial cells
    Stephen C M Tsoi
    Department of Obstetrics and Gynecology, University of Wisconsin Madison, 7E Meriter Hospital, 202 S Park St, Madison, WI 53715, USA
    Mol Cell Endocrinol 196:95-106. 2002
    ..Together with expression of VEGF and VEGF receptors in OFPAE cells, we propose that there is an autocrine mechanism by which VEGF regulates fetal placental angiogenesis and other functions of endothelial cells...
  33. ncbi request reprint ERp19 and ERp46, new members of the thioredoxin family of endoplasmic reticulum proteins
    Barbara Knoblach
    Canadian Institutes of Health Research Membrane Protein Research Group and the Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Mol Cell Proteomics 2:1104-19. 2003
    ..Functional analysis with yeast complementation studies showed that ERp46 but not ERp19 can substitute for protein disulphide isomerase function in vivo...
  34. ncbi request reprint Identification of mammalian proteins cross-linked to DNA by ionizing radiation
    Sharon Barker
    Department of Oncology, Cross Cancer Institute, Edmonton, Alberta T6G 1Z2
    J Biol Chem 280:33826-38. 2005
    ..Quantitation of the fluorescence signal indicated no significant difference in total yields of IR-induced DPCs generated under aerated or hypoxic conditions, although differences were observed for several individual protein bands...
  35. ncbi request reprint Characterization of human tear proteome using multiple proteomic analysis techniques
    Nan Li
    Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
    J Proteome Res 4:2052-61. 2005
    ..This work represents one of the most extensive proteome profiles (i.e., proteins identified and PTMs characterized) generated from tear fluids using clinically relevant amounts of sample...
  36. pmc The timing of endocytosis after activation of a G-protein-coupled receptor in a sensory neuron
    Lie Cheng Wang
    Institute of Molecular Medicine, Peking University, Beijing, China
    Biophys J 90:3590-8. 2006
    ..Finally, the receptors were internalized in response to ADP, as determined by GFP-labeled P2Y. We conclude that ligand-receptor binding leads to rapid endocytosis in the cytoplasm of rat dorsal root ganglion neurons...
  37. ncbi request reprint Keratinocyte-releasable stratifin functions as a potent collagenase-stimulating factor in fibroblasts
    Aziz Ghahary
    Department of Surgery, Wound Healing Research Group, University of Alberta, Edmonton, Alberta, Canada
    J Invest Dermatol 122:1188-97. 2004
    ..This finding suggests that stratifin is likely to be, at least, one of the KDAFs found in KCM...
  38. ncbi request reprint A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor
    Yong Quan Li
    College of Life Science, Zhejiang University, Hongzhou 310027, China
    J Zhejiang Univ Sci 5:173-9. 2004
    ..Thus, these experiment results confirmed that the two-component system ecrA1/A2 was positive regulatory element for red gene cluster...
  39. pmc Establishment of a functional ovine fetoplacental artery endothelial cell line with a prolonged life span
    Yang Song
    Department of Obstetrics and Gynecology, Perinatal Research Laboratories, University of Wisconsin, Madison, Wisconsin 53715, USA
    Biol Reprod 76:29-35. 2007
    ..Thus, a functional OFPAE cell line with an extended life span has been successfully established, potentially providing a valuable cell model for studying fetoplacental endothelial function...
  40. ncbi request reprint Cellular interacting proteins of functional screen-derived antiproliferative and cytotoxic peptides discovered using shotgun peptide sequencing
    Tarikere Gururaja
    Rigel Incorporated, South San Francisco, CA 94080, USA
    Chem Biol 10:927-37. 2003
  41. ncbi request reprint Development and applications of in-gel CNBr/tryptic digestion combined with mass spectrometry for the analysis of membrane proteins
    Thuy Tien T Quach
    Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2
    J Proteome Res 2:543-52. 2003
    ..The results are presented from a study of the analysis of bacteriorhodopsin, nitrate reductase 1 gamma chain, and a complex protein mixture extracted from the endoplasmic recticulum membrane of mouse liver...
  42. ncbi request reprint Membrane estrogen receptor-dependent extracellular signal-regulated kinase pathway mediates acute activation of endothelial nitric oxide synthase by estrogen in uterine artery endothelial cells
    Dong Bao Chen
    Department of Reproductive Medicine, University of California San Diego, La Jolla, California 92093 0802, USA
    Endocrinology 145:113-25. 2004
    ..This pathway may provide a novel mechanism for NO-mediated rapid uterine vasodilatation by estrogen...
  43. ncbi request reprint Phosphorylation of RasGRP3 on threonine 133 provides a mechanistic link between PKC and Ras signaling systems in B cells
    Yong Zheng
    Department of Biochemistry, University of Alberta, Edmonton AB, Canada
    Blood 105:3648-54. 2005
    ..PKC-mediated regulation of RasGRPs in lymphocytes may generate cooperative signaling in response to increases in DAG. The mast- and myeloid-selective family member RasGRP4 is regulated by different means...

Research Grants1

  1. Investigating the nature of prestin-associated proteins
    Jing Zheng; Fiscal Year: 2007
    ..This knowledge may make it possible to manipulate OHC function for therapeutic purposes. ..