Michael C Jewett

Summary

Affiliation: Northwestern University
Country: USA

Publications

  1. pmc Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes
    Filippo Caschera
    Department of Chemical and Biochemical Engineering, Chemistry of Life Processes Institute, Simpson Querrey Institute for BioNanotechnology, Northwestern University, Evanston, 60208, Illinois, USA
    Chem Commun (Camb) 52:5467-9. 2016
  2. pmc In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation
    Michael C Jewett
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Syst Biol 9:678. 2013
  3. pmc Update on designing and building minimal cells
    Michael C Jewett
    Department of Chemical and Biological Engineering and Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Curr Opin Biotechnol 21:697-703. 2010
  4. pmc Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins
    Rui Gan
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL, 60208
    Biotechnol Bioeng . 2016
  5. pmc The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Nucleic Acids Res 42:6774-85. 2014
  6. doi request reprint Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis
    C Eric Hodgman
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Biotechnol Bioeng 110:2643-54. 2013
  7. pmc Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1
    Seok Hoon Hong
    Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Tech E 136, Evanston, IL 60208 USA
    Chembiochem 16:844-53. 2015
  8. doi request reprint Characterizing and alleviating substrate limitations for improved in vitro ribosome construction
    Yi Liu
    Interdepartmental Biological Sciences Graduate Program, Chemistry of Life Processes Institute, Department of Chemical and Biological Engineering, Member, Robert H Lurie Comprehensive Cancer Center, Affiliate Member, Institute for BioNanotechnology in Medicine, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States
    ACS Synth Biol 4:454-62. 2015
  9. pmc Biology by design: from top to bottom and back
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    J Biomed Biotechnol 2010:232016. 2010
  10. doi request reprint Establishing a high yielding Streptomyces-based cell-free protein synthesis system
    Jian Li
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208
    Biotechnol Bioeng . 2017

Collaborators

  • Farren J Isaacs
  • Neil L Kelleher
  • Jian Li
  • George M Church
  • Adrian D Haimovich
  • Andrew C Tolonen
  • Duhee Bang
  • Harris H Wang
  • Peter A Carr
  • Anthony C Forster
  • Rui Gan
  • C Eric Hodgman
  • Brian R Fritz
  • Adam J Hockenberry
  • Mark J Anderson
  • Seok Hoon Hong
  • Jessica G Perez
  • Ioanna Ntai
  • Benjamin J Des Soye
  • Jennifer A Schoborg
  • Erik D Carlson
  • Anthony W Goering
  • Luis A N Amaral
  • Sophia S Liu
  • Filippo Caschera
  • Jessica C Stark
  • Yi Liu
  • Javin P Oza
  • D Calvin Harris
  • Regan J Thomson
  • Ryan A McClure
  • Adam R Pah
  • Kenneth K Y Ho
  • Andrea Lancichinetti
  • Allen P Liu
  • Jin Woo Lee
  • Yong Chan Kwon
  • Jaymin R Patel
  • Rey W Martin
  • Kirsten N Swonger
  • Jesse Rinehart
  • Charlotte M ter Haar
  • Matthew B Amrofell
  • Osman K Jamil
  • Natasha L Pirman
  • Alexandra M de Paz
  • Karl W Barber
  • Hans R Aerni
  • Svetlana Rogulina
  • Joshua N Leonard
  • Laura E Timmerman
  • Nichole M Daringer

Detail Information

Publications28

  1. pmc Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes
    Filippo Caschera
    Department of Chemical and Biochemical Engineering, Chemistry of Life Processes Institute, Simpson Querrey Institute for BioNanotechnology, Northwestern University, Evanston, 60208, Illinois, USA
    Chem Commun (Camb) 52:5467-9. 2016
    ..Two different liposome phospholipid formulations were investigated to characterize their effects on the compartmentalized reaction kinetics. This study was performed as a necessary step towards the synthesis of minimal cells. ..
  2. pmc In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation
    Michael C Jewett
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Syst Biol 9:678. 2013
    ..We anticipate that iSAT will aid studies of ribosome assembly and open new avenues for making ribosomes with altered properties. ..
  3. pmc Update on designing and building minimal cells
    Michael C Jewett
    Department of Chemical and Biological Engineering and Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Curr Opin Biotechnol 21:697-703. 2010
    ..Major progress in the past 5 years has occurred in synthetic genomics, minimization of the Escherichia coli genome, sequencing of minimal bacterial endosymbionts, identification of essential genes, and integration of biochemical systems...
  4. pmc Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins
    Rui Gan
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL, 60208
    Biotechnol Bioeng . 2016
    ..This article is protected by copyright. All rights reserved...
  5. pmc The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Nucleic Acids Res 42:6774-85. 2014
    ..coli cells. Looking forward, we believe iSAT will enable unique studies to unravel the systems biology of ribosome biogenesis and open the way to new methods for making and studying ribosomal variants. ..
  6. doi request reprint Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis
    C Eric Hodgman
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Biotechnol Bioeng 110:2643-54. 2013
    ..Our results set the stage for developing a yeast CFPS platform that provides for high-yielding and cost-effective expression of a variety of protein therapeutics and protein libraries...
  7. pmc Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1
    Seok Hoon Hong
    Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Tech E 136, Evanston, IL 60208 USA
    Chembiochem 16:844-53. 2015
    ..Our work has implications for using whole genome editing for CFPS strain development, expanding the chemistry of biological systems, and cell-free synthetic biology...
  8. doi request reprint Characterizing and alleviating substrate limitations for improved in vitro ribosome construction
    Yi Liu
    Interdepartmental Biological Sciences Graduate Program, Chemistry of Life Processes Institute, Department of Chemical and Biological Engineering, Member, Robert H Lurie Comprehensive Cancer Center, Affiliate Member, Institute for BioNanotechnology in Medicine, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States
    ACS Synth Biol 4:454-62. 2015
    ..Our results underscore the critical role energy substrates play in iSAT and highlight the importance of understanding metabolic processes that influence substrate depletion for cell-free synthetic biology...
  9. pmc Biology by design: from top to bottom and back
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    J Biomed Biotechnol 2010:232016. 2010
    ..Ten years after the construction of synthetic biology's first devices, the drive to look beyond what does exist to what can exist is ushering in an era of biology by design...
  10. doi request reprint Establishing a high yielding Streptomyces-based cell-free protein synthesis system
    Jian Li
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, 60208
    Biotechnol Bioeng . 2017
    ..g., nonribosomal peptides and polyketides), providing a new avenue for obtaining and studying natural product biosynthesis pathways. This article is protected by copyright. All rights reserved...
  11. pmc Leveraging genome-wide datasets to quantify the functional role of the anti-Shine-Dalgarno sequence in regulating translation efficiency
    Adam J Hockenberry
    Interdisciplinary Program in Biological Sciences, Northwestern University, Evanston, IL 60208, USA
    Open Biol 7:. 2017
    ..Further, we verify our main conclusions by re-analysing a controlled experimental dataset...
  12. pmc Cell-Free Synthetic Biology: Engineering Beyond the Cell
    Jessica G Perez
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208 3120
    Cold Spring Harb Perspect Biol 8:. 2016
    ..Given the flexibility of cell-free systems, CFPS holds promise to be a powerful tool for synthetic biology as well as a protein production technology in years to come...
  13. pmc In Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis
    Anthony W Goering
    Department of Molecular Biosciences, and the Feinberg School of Medicine, Department of Chemistry, and Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States
    ACS Synth Biol 6:39-44. 2017
    ..Overall, this work represents a first step to apply cell-free protein synthesis to discover and characterize new natural products...
  14. pmc Cell-free protein synthesis: applications come of age
    Erik D Carlson
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Biotechnol Adv 30:1185-94. 2012
    ..In the coming years, cell-free protein synthesis promises new industrial processes where short protein production timelines are crucial as well as innovative approaches to a wide range of applications...
  15. doi request reprint Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase
    Jian Li
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA
    Biotechnol J 11:212-8. 2016
    ..Our work extends the utility of the cell-free platform to the production of active proteins containing copper cofactors and demonstrates a simple method for producing MCOs. ..
  16. pmc Repurposing the translation apparatus for synthetic biology
    Benjamin J Des Soye
    Interdisciplinary Biological Sciences Program, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA Northwestern Institute on Complex Systems, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA Simpson Querrey Institute, Northwestern University, 303 East Superior Street, Suite 11 131, Chicago, IL 60611, USA Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Curr Opin Chem Biol 28:83-90. 2015
    ..The transformative potential of repurposing the translation apparatus has emerged as one of the defining opportunities at the interface of chemical and synthetic biology. ..
  17. pmc Energizing eukaryotic cell-free protein synthesis with glucose metabolism
    Mark J Anderson
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Technological Institute E136, Evanston, IL 60208 3120, USA Chemistry of Life Processes Institute, Northwestern University, 2170 Campus Drive, Evanston, IL 60208 3120, USA
    FEBS Lett 589:1723-7. 2015
    ..Our demonstration provides the foundation for development of cost-effective eukaryotic CFPS platforms...
  18. pmc NullSeq: A Tool for Generating Random Coding Sequences with Desired Amino Acid and GC Contents
    Sophia S Liu
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, United States of America
    PLoS Comput Biol 12:e1005184. 2016
    ....
  19. doi request reprint Evolution of translation initiation sequences using in vitro yeast ribosome display
    Rui Gan
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, Illinois, 60208
    Biotechnol Bioeng 113:1777-86. 2016
    ..Looking forward, yeast ribosome display holds promise for evolving libraries of proteins and DNA regulatory parts for protein engineering and synthetic biology. Biotechnol. Bioeng. 2016;113: 1777-1786. © 2016 Wiley Periodicals, Inc. ..
  20. pmc Robust production of recombinant phosphoproteins using cell-free protein synthesis
    Javin P Oza
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA
    Nat Commun 6:8168. 2015
    ..This work will facilitate study of phosphorylation-dependent structure-function relationships, kinase signalling networks and kinase inhibitor drugs. ..
  21. pmc Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction
    Brian R Fritz
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Nucleic Acids Res 43:4774-84. 2015
    ....
  22. doi request reprint Characterizing IGR IRES-mediated translation initiation for use in yeast cell-free protein synthesis
    C Eric Hodgman
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Technological Institute, E136, Evanston, IL 60208 3120, USA Chemistry of Life Processes Institute, Northwestern University, 2170 Campus Drive, Evanston, IL 60208 3120, USA
    N Biotechnol 31:499-505. 2014
    ..This study highlights the crucial role translation initiation plays in yeast CFPS and offers a simple platform to study IRES sequences...
  23. pmc Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation
    Seok Hoon Hong
    Department of Chemical and Biological Engineering, Chemistry of Life Processes Institute, Department of Chemistry, and Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208, United States of America
    ACS Synth Biol 3:398-409. 2014
    ..Looking forward, using RF1-deficient strains for extract-based CFPS will aid in the synthesis of proteins and biopolymers with site-specifically incorporated NSAAs...
  24. doi request reprint Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis
    Jennifer A Schoborg
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA
    Biotechnol J 9:630-40. 2014
    ..This study identifies targets for the future improvement of the batch yeast CFPS reaction. Moreover, it outlines a detailed, generalized method to characterize and improve other CFPS platforms. ..
  25. doi request reprint A combined cell-free transcription-translation system from Saccharomyces cerevisiae for rapid and robust protein synthe
    Rui Gan
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA
    Biotechnol J 9:641-51. 2014
    ..See accompanying commentary by Russ and Dueber DOI: 10.1002/biot.201400071. ..
  26. doi request reprint Precise manipulation of chromosomes in vivo enables genome-wide codon replacement
    Farren J Isaacs
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Science 333:348-53. 2011
    ..Our methods treat the chromosome as both an editable and an evolvable template, permitting the exploration of vast genetic landscapes...
  27. pmc Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry
    D Calvin Harris
    Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA
    Curr Opin Biotechnol 23:672-8. 2012
    ..In the coming years, the impact of advances inspired by these approaches will make possible the synthesis of nonbiological polymers having new backbone compositions, new chemical properties, new structures, and new functions...
  28. pmc Synthetic in vitro circuits
    Adam J Hockenberry
    Interdepartmental Biological Sciences Graduate Program, Northwestern University, Evanston, IL 60208, USA
    Curr Opin Chem Biol 16:253-9. 2012
    ..Progress in this field is showing that cell-free synthetic biology is a versatile testing ground for understanding native biological circuits and engineering novel functionality...