David Metzgar

Summary

Affiliation: Naval Medical Center
Country: USA

Publications

  1. pmc Rapid detection and molecular serotyping of adenovirus by use of PCR followed by electrospray ionization mass spectrometry
    Lawrence B Blyn
    Ibis Biosciences, Inc, Carlsbad, CA 92008, USA
    J Clin Microbiol 46:644-51. 2008
  2. pmc Co-infections of adenovirus species in previously vaccinated patients
    Gary J Vora
    Naval Research Laboratory, Washington, DC, USA
    Emerg Infect Dis 12:921-30. 2006
  3. pmc PCR analysis of egyptian respiratory adenovirus isolates, including identification of species, serotypes, and coinfections
    David Metzgar
    Naval Health Research Center, P O Box 85122, San Diego, CA 92186 5122, USA
    J Clin Microbiol 43:5743-52. 2005
  4. doi request reprint Broad spectrum respiratory pathogen analysis of throat swabs from military recruits reveals interference between rhinoviruses and adenoviruses
    Zheng Wang
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA
    Microb Ecol 59:623-34. 2010
  5. pmc Using a resequencing microarray as a multiple respiratory pathogen detection assay
    Baochuan Lin
    Center for Bio Molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, DC 20375, USA
    J Clin Microbiol 45:443-52. 2007
  6. doi request reprint Local changes in rates of group A Streptococcus disease and antibiotic resistance are associated with geographically widespread strain turnover events
    David Metzgar
    Department of Respiratory Diseases Research, Naval Health Research Center, San Diego, CA, USA
    Virulence 1:247-53. 2010
  7. pmc A cluster of Legionella-associated pneumonia cases in a population of military recruits
    Erin A McDonough
    Department of Defence Center for Deployment Health Research, Naval Health Research Center, San Diego, CA 92186 5122, USA
    J Clin Microbiol 45:2075-7. 2007
  8. pmc Single assay for simultaneous detection and differential identification of human and avian influenza virus types, subtypes, and emergent variants
    David Metzgar
    Department of Respiratory Diseases Research, Naval Health Research Center, San Diego, California, United States of America
    PLoS ONE 5:e8995. 2010
  9. ncbi request reprint Abrupt emergence of diverse species B adenoviruses at US military recruit training centers
    David Metzgar
    Respiratory Disease Laboratory, Department of Defense Center for Deployment Health Research, Naval Health Research Center, San Diego, CA 92186, USA
    J Infect Dis 196:1465-73. 2007
  10. pmc Evaluation and validation of a real-time PCR assay for detection and quantitation of human adenovirus 14 from clinical samples
    David Metzgar
    Department of Respiratory Disease Research, Naval Health Research Center, San Diego, CA, USA
    PLoS ONE 4:e7081. 2009

Collaborators

Detail Information

Publications26

  1. pmc Rapid detection and molecular serotyping of adenovirus by use of PCR followed by electrospray ionization mass spectrometry
    Lawrence B Blyn
    Ibis Biosciences, Inc, Carlsbad, CA 92008, USA
    J Clin Microbiol 46:644-51. 2008
    ..Over 500 samples per day can be analyzed with sensitivities greater than 100 genomes per reaction. This approach can be applied to many other families of infectious agents for rapid and sensitive analysis...
  2. pmc Co-infections of adenovirus species in previously vaccinated patients
    Gary J Vora
    Naval Research Laboratory, Washington, DC, USA
    Emerg Infect Dis 12:921-30. 2006
    ..This study highlights the rarely reported phenomenon of adenoviral co-infections in a clinically relevant environment suitable for the generation of new recombinational variants...
  3. pmc PCR analysis of egyptian respiratory adenovirus isolates, including identification of species, serotypes, and coinfections
    David Metzgar
    Naval Health Research Center, P O Box 85122, San Diego, CA 92186 5122, USA
    J Clin Microbiol 43:5743-52. 2005
    ..In particular, coinfections may influence adenoviral evolution, as interserotypic recombination has been identified as a source of emerging strains...
  4. doi request reprint Broad spectrum respiratory pathogen analysis of throat swabs from military recruits reveals interference between rhinoviruses and adenoviruses
    Zheng Wang
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA
    Microb Ecol 59:623-34. 2010
    ..The results showed a strong negative association between adenoviral FRI and the presence of rhinoviruses in recruits, suggesting some form of viral interference...
  5. pmc Using a resequencing microarray as a multiple respiratory pathogen detection assay
    Baochuan Lin
    Center for Bio Molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, DC 20375, USA
    J Clin Microbiol 45:443-52. 2007
    ..Successful proof-of-concept experiments using clinical specimens show that this approach is potentially very useful for both diagnostics and epidemic surveillance...
  6. doi request reprint Local changes in rates of group A Streptococcus disease and antibiotic resistance are associated with geographically widespread strain turnover events
    David Metzgar
    Department of Respiratory Diseases Research, Naval Health Research Center, San Diego, CA, USA
    Virulence 1:247-53. 2010
    ..Shifts in antibiotic resistance profiles and disease rates at specific sites appear to be associated with strain replacements happening on larger scales, independent of antibiotic use at individual sites...
  7. pmc A cluster of Legionella-associated pneumonia cases in a population of military recruits
    Erin A McDonough
    Department of Defence Center for Deployment Health Research, Naval Health Research Center, San Diego, CA 92186 5122, USA
    J Clin Microbiol 45:2075-7. 2007
    ..S. military recruits during population-based surveillance for pneumonia pathogens. Results were confirmed by sequence analysis. Cases clustered tightly, suggesting a local environmental etiology...
  8. pmc Single assay for simultaneous detection and differential identification of human and avian influenza virus types, subtypes, and emergent variants
    David Metzgar
    Department of Respiratory Diseases Research, Naval Health Research Center, San Diego, California, United States of America
    PLoS ONE 5:e8995. 2010
    ..The informative specificity of a single RPM-Flu test represents specimen-specific viral gene sequences as determinants of virus type, A/HN subtype, virulence, host-range, and resistance to antiviral agents...
  9. ncbi request reprint Abrupt emergence of diverse species B adenoviruses at US military recruit training centers
    David Metzgar
    Respiratory Disease Laboratory, Department of Defense Center for Deployment Health Research, Naval Health Research Center, San Diego, CA 92186, USA
    J Infect Dis 196:1465-73. 2007
    ..Between 1999 and 2002, serotype 4 accounted for >95% of Ads isolated from recruits and for >50% of ARD cases in training facilities (15,000 cases/year)...
  10. pmc Evaluation and validation of a real-time PCR assay for detection and quantitation of human adenovirus 14 from clinical samples
    David Metzgar
    Department of Respiratory Disease Research, Naval Health Research Center, San Diego, CA, USA
    PLoS ONE 4:e7081. 2009
    ..The described assay is easy to use, sensitive and specific for HAdV-14a in clinical throat swab specimens, and very rapid since turnaround time is less than four hours to obtain an answer...
  11. pmc Identifying influenza viruses with resequencing microarrays
    Zheng Wang
    Naval Research Laboratory, Washington, DC, USA
    Emerg Infect Dis 12:638-46. 2006
    ..The results demonstrate a novel, timely, and unbiased method for the molecular epidemiologic surveillance of influenza viruses...
  12. pmc Universal detection and identification of avian influenza virus by use of resequencing microarrays
    Baochuan Lin
    Center for Bio Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA
    J Clin Microbiol 47:988-93. 2009
    ....
  13. pmc Application of broad-spectrum, sequence-based pathogen identification in an urban population
    Baochuan Lin
    Center for Bio Molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, DC, United States of America
    PLoS ONE 2:e419. 2007
    ....
  14. pmc Use of oligonucleotide microarrays for rapid detection and serotyping of acute respiratory disease-associated adenoviruses
    Baochuan Lin
    Center for Bio Molecular Science and Engineering, Code 6900, Naval Research Laboratory, 4555 Overlook Ave, SW, Bldg 30, Washington, DC 20375, USA
    J Clin Microbiol 42:3232-9. 2004
    ..5 to 4.0 h...
  15. pmc Evaluation of multiplex type-specific real-time PCR assays using the LightCycler and joint biological agent identification and diagnostic system platforms for detection and quantitation of adult human respiratory adenoviruses
    David Metzgar
    Department of Respiratory Diseases Research, Naval Health Research Center, San Diego, California 92152, USA
    J Clin Microbiol 48:1397-403. 2010
    ..These assays have the potential to be useful as clinical diagnostic tools for the detection of HAdV infection in adult populations...
  16. pmc Inference of antibiotic resistance and virulence among diverse group A Streptococcus strains using emm sequencing and multilocus genotyping methods
    David Metzgar
    Department of Respiratory Diseases Research, Naval Health Research Center, San Diego, California, United States of America
    PLoS ONE 4:e6897. 2009
    ..Phenotype analysis, including critical AbR typing, is generally achieved by much slower and more laborious direct culture-based methods...
  17. doi request reprint Genomic characterization of human adenovirus 36, a putative obesity agent
    John Arnold
    Department of Pediatrics, Division of Infectious Diseases, Naval Medical Center San Diego, San Diego, CA, USA
    Virus Res 149:152-61. 2010
    ..Furthermore, the predicted molecular models of the HAdV-D36 fiber protein seem to implicate a unique tissue tropism for HAdV-D36...
  18. pmc Broad-spectrum respiratory tract pathogen identification using resequencing DNA microarrays
    Baochuan Lin
    Center for Bio Molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, District of Columbia 20375, USA
    Genome Res 16:527-35. 2006
    ..Our results underscore the feasibility of using this approach to expedite the early surveillance of diseases, and provide new information on the incidence of multiple pathogens...
  19. ncbi request reprint Diagnostic discrimination of live attenuated influenza vaccine strains and community-acquired pathogenic strains in clinical samples
    Nikki E Freed
    Department of Defense Center for Deployment Health Research, Naval Health Research Center, San Diego, CA 92186 5122, USA
    Mol Cell Probes 21:103-10. 2007
    ..The results show that the test is effective, and demonstrate the importance of such tests in the age of live vaccines...
  20. pmc Initial identification and characterization of an emerging zoonotic influenza virus prior to pandemic spread
    David Metzgar
    Department of Respiratory Disease Research, Naval Health Research Center, San Diego, California, USA
    J Clin Microbiol 48:4228-34. 2010
    ..Here we describe the initial characterization of the pandemic 2009/H1N1 influenza strain and discuss the possible roles of diagnostic tools with discovery potential...
  21. pmc Molecular analysis of adenovirus isolates from vaccinated and unvaccinated young adults
    Daniel A Blasiole
    Department of Defense Center for Deployment Health Research, Naval Health Research Center, San Diego, California 92186 5122, USA
    J Clin Microbiol 42:1686-93. 2004
    ....
  22. pmc Identification of a novel human papillomavirus by metagenomic analysis of samples from patients with febrile respiratory illness
    John L Mokili
    Department of Biology, San Diego State University, San Diego, California, USA
    PLoS ONE 8:e58404. 2013
    ..The metagenomic procedures, coupled with the bioinformatic methods described herein are well suited to detect small circular genomes such as those of human papillomaviruses...
  23. ncbi request reprint A multiplex PCR for detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in clinical specimens
    Erin A McDonough
    Department of Defense Center for Deployment Health Research, Naval Health Research Center, P O Box 85122, San Diego, CA 92186 5122, USA
    Mol Cell Probes 19:314-22. 2005
    ..Given this, the overall impact of these pathogens on public health may be grossly underestimated. The molecular test presented here provides a simple method for identification of four common, yet diagnostically challenging, pathogens...
  24. ncbi request reprint Molecular epidemiology of adenovirus type 4 infections in US military recruits in the postvaccination era (1997-2003)
    Adriana E Kajon
    Infectious Disease Program, Lovelace Respiratory Research Institute, Albuquerque, NM 87108, USA
    J Infect Dis 196:67-75. 2007
    ..The discontinuation of Ad vaccination programs in 1999 precipitated the reemergence of Ad in training sites, with Ad4 accounting for 98% of all diagnosed cases...
  25. doi request reprint A double-blind, placebo-controlled study of the safety and immunogenicity of live, oral type 4 and type 7 adenovirus vaccines in adults
    Arthur Lyons
    Department of Virus Diseases, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, MD, USA
    Vaccine 26:2890-8. 2008
    ..3) while 63% seroconverted to ADV-7 (GMT 51.1) by Day 28. The new ADV-4 and ADV-7 vaccines were safe and induced a good immune response in the study population. Expanded trials for safety and efficacy are in progress...
  26. pmc Acinetobacter sp. ADP1: an ideal model organism for genetic analysis and genome engineering
    David Metzgar
    The Scripps Research Institute, BCC 379, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA
    Nucleic Acids Res 32:5780-90. 2004
    ..They are also compatible with complete automation of genetic analysis and engineering protocols...