Huanchen Wang

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Structural basis for an inositol pyrophosphate kinase surmounting phosphate crowding
    Huanchen Wang
    Inositol Signaling Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA
    Nat Chem Biol 8:111-6. 2012
  2. pmc The kinetic properties of a human PPIP5K reveal that its kinase activities are protected against the consequences of a deteriorating cellular bioenergetic environment
    Jeremy D Weaver
    Inositol Signaling Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, P O Box 12233, NC 27709, U S A
    Biosci Rep 33:e00022. 2013
  3. pmc Synthetic inositol phosphate analogs reveal that PPIP5K2 has a surface-mounted substrate capture site that is a target for drug discovery
    Huanchen Wang
    Inositol Signaling Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Chem Biol 21:689-99. 2014
  4. pmc Structural insight into inositol pyrophosphate turnover
    Stephen B Shears
    Inositol Signaling Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, PO Box 12233, NC 27709, USA
    Adv Biol Regul 53:19-27. 2013
  5. pmc Asp1 from Schizosaccharomyces pombe binds a [2Fe-2S](2+) cluster which inhibits inositol pyrophosphate 1-phosphatase activity
    Huanchen Wang
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, 101 T W Alexander Drive, Research Triangle Park, North Carolina 27709, United States
    Biochemistry 54:6462-74. 2015
  6. pmc IP6K structure and the molecular determinants of catalytic specificity in an inositol phosphate kinase family
    Huanchen Wang
    Inositol Signaling Group, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, NC 27709, USA
    Nat Commun 5:4178. 2014

Collaborators

Detail Information

Publications6

  1. pmc Structural basis for an inositol pyrophosphate kinase surmounting phosphate crowding
    Huanchen Wang
    Inositol Signaling Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA
    Nat Chem Biol 8:111-6. 2012
    ..We describe the enzyme's conformational dynamics, its unprecedented topological presentation of nucleotide and inositol phosphate, and the charge balance that facilitates partly associative in-line phosphoryl transfer...
  2. pmc The kinetic properties of a human PPIP5K reveal that its kinase activities are protected against the consequences of a deteriorating cellular bioenergetic environment
    Jeremy D Weaver
    Inositol Signaling Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, P O Box 12233, NC 27709, U S A
    Biosci Rep 33:e00022. 2013
    ..Finally, the specificity constants for PPIP5K2 revise upwards by one-to-two orders of magnitude the inherent catalytic activities of this enzyme, and we show its equilibrium point favours 80-90% depletion of InsP₆/₅-InsP₇...
  3. pmc Synthetic inositol phosphate analogs reveal that PPIP5K2 has a surface-mounted substrate capture site that is a target for drug discovery
    Huanchen Wang
    Inositol Signaling Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Chem Biol 21:689-99. 2014
    ..This work suggests that the substrate-binding site offers new opportunities for targeted drug design. ..
  4. pmc Structural insight into inositol pyrophosphate turnover
    Stephen B Shears
    Inositol Signaling Group, Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, PO Box 12233, NC 27709, USA
    Adv Biol Regul 53:19-27. 2013
    ..This work includes the analysis of crystal complexes with substrates, products, transition state analogs, and a novel phosphonoacetate substrate analog...
  5. pmc Asp1 from Schizosaccharomyces pombe binds a [2Fe-2S](2+) cluster which inhibits inositol pyrophosphate 1-phosphatase activity
    Huanchen Wang
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, 101 T W Alexander Drive, Research Triangle Park, North Carolina 27709, United States
    Biochemistry 54:6462-74. 2015
    ..Biochemical and mutagenic studies demonstrated that the [2Fe-2S](2+) cluster substantially inhibits the phosphatase activity of Asp1, thereby increasing its net kinase activity. ..
  6. pmc IP6K structure and the molecular determinants of catalytic specificity in an inositol phosphate kinase family
    Huanchen Wang
    Inositol Signaling Group, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, NC 27709, USA
    Nat Commun 5:4178. 2014
    ..These data reveal the molecular determinants of IP6K activity, and suggest an unusual evolutionary trajectory for a primordial kinase that could have favored efficient bifunctionality, before propagation of separate IP3Ks and IP6Ks. ..