James R Sellers

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. doi request reprint Folding and regulation in myosins II and V
    James R Sellers
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Building 50, Room 3523, Bethesda, MD 20892, USA
    J Muscle Res Cell Motil 28:363-70. 2007
  2. ncbi request reprint Fifty years of contractility research post sliding filament hypothesis
    James R Sellers
    Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Muscle Res Cell Motil 25:475-82. 2004
  3. ncbi request reprint Walking with myosin V
    James R Sellers
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    Curr Opin Cell Biol 18:68-73. 2006
  4. ncbi request reprint Myosin V from Drosophila reveals diversity of motor mechanisms within the myosin V family
    Judit Toth
    Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    J Biol Chem 280:30594-603. 2005
  5. ncbi request reprint Myosin VIIB from Drosophila is a high duty ratio motor
    Yi Yang
    Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    J Biol Chem 280:32061-8. 2005
  6. ncbi request reprint Disease-associated mutations and alternative splicing alter the enzymatic and motile activity of nonmuscle myosins II-B and II-C
    Kye Young Kim
    Laboratory of Molecular Cardiology and Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:22769-75. 2005
  7. pmc Mammalian myosin-18A, a highly divergent myosin
    Stephanie Guzik-Lendrum
    Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, MD 20892 8015, USA
    J Biol Chem 288:9532-48. 2013
  8. ncbi request reprint Mechanism of action of myosin X, a membrane-associated molecular motor
    Mihaly Kovacs
    Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    J Biol Chem 280:15071-83. 2005
  9. ncbi request reprint In vitro reconstitution of a transport complex containing Rab27a, melanophilin and myosin Va
    Xufeng Wu
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Building 50 Room 2523 NIH 9000 Rockvill, Bethesda, MD 20892 8017, USA
    FEBS Lett 580:5863-8. 2006
  10. pmc An alternatively spliced isoform of non-muscle myosin II-C is not regulated by myosin light chain phosphorylation
    Siddhartha S Jana
    Laboratory of Molecular Cardiology and Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 284:11563-71. 2009

Collaborators

Detail Information

Publications67

  1. doi request reprint Folding and regulation in myosins II and V
    James R Sellers
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Building 50, Room 3523, Bethesda, MD 20892, USA
    J Muscle Res Cell Motil 28:363-70. 2007
    ..In myosin V the key feature is an interaction between the heads and the globular tail domain...
  2. ncbi request reprint Fifty years of contractility research post sliding filament hypothesis
    James R Sellers
    Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Muscle Res Cell Motil 25:475-82. 2004
  3. ncbi request reprint Walking with myosin V
    James R Sellers
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    Curr Opin Cell Biol 18:68-73. 2006
    ..The mechanism by which myosin V moves processively along actin filaments has been the subject of many biophysical and biochemical studies and a consensus is starting to emerge about how this minute molecular motor operates...
  4. ncbi request reprint Myosin V from Drosophila reveals diversity of motor mechanisms within the myosin V family
    Judit Toth
    Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    J Biol Chem 280:30594-603. 2005
    ..Our comparison between vertebrate and fly myosin V demonstrates that the well preserved function of myosin V motors in cytoplasmic transport can be accomplished by markedly different underlying mechanisms...
  5. ncbi request reprint Myosin VIIB from Drosophila is a high duty ratio motor
    Yi Yang
    Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    J Biol Chem 280:32061-8. 2005
    ..Our experiments also revealed conformational heterogeneity of the actomyosin VIIB complex in the absence of nucleotide...
  6. ncbi request reprint Disease-associated mutations and alternative splicing alter the enzymatic and motile activity of nonmuscle myosins II-B and II-C
    Kye Young Kim
    Laboratory of Molecular Cardiology and Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:22769-75. 2005
    ..Insertion of eight amino acids into the HMM II-C heavy chain increases both actin-activated MgATPase activity and in vitro motility...
  7. pmc Mammalian myosin-18A, a highly divergent myosin
    Stephanie Guzik-Lendrum
    Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, MD 20892 8015, USA
    J Biol Chem 288:9532-48. 2013
    ..These data lead us to conclude that myosin-18A does not operate as a traditional molecular motor in cells...
  8. ncbi request reprint Mechanism of action of myosin X, a membrane-associated molecular motor
    Mihaly Kovacs
    Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    J Biol Chem 280:15071-83. 2005
    ..ADP. The nucleotide-free complex of myosin X with actin shows unique spectral and biochemical characteristics, indicating a special mode of actomyosin interaction...
  9. ncbi request reprint In vitro reconstitution of a transport complex containing Rab27a, melanophilin and myosin Va
    Xufeng Wu
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Building 50 Room 2523 NIH 9000 Rockvill, Bethesda, MD 20892 8017, USA
    FEBS Lett 580:5863-8. 2006
    ....
  10. pmc An alternatively spliced isoform of non-muscle myosin II-C is not regulated by myosin light chain phosphorylation
    Siddhartha S Jana
    Laboratory of Molecular Cardiology and Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 284:11563-71. 2009
    ..This is in contrast to NM II-C1 and NM II-C0, both of which are ubiquitously expressed. Full-length NM II-C2-GFP expressed in COS-7 cells localizes to filaments in interphase cells and to the cytokinetic ring in dividing cells...
  11. ncbi request reprint Functional divergence of human cytoplasmic myosin II: kinetic characterization of the non-muscle IIA isoform
    Mihaly Kovacs
    Laboratory of Molecular Cardiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    J Biol Chem 278:38132-40. 2003
    ..Non-muscle myosin II isoforms thus appear to have distinct enzymatic properties that may be of importance in carrying out their cellular functions...
  12. doi request reprint Mutation spectrum of MYO7A and evaluation of a novel nonsyndromic deafness DFNB2 allele with residual function
    Saima Riazuddin
    Section on Human Genetics, Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Rockville, Maryland 20850, USA
    Hum Mutat 29:502-11. 2008
    ..This finding is consistent with the hypothesis that p.E1716del causes a less severe phenotype (DFNB2) than the USH1B-associated alleles because the resulting protein retains some degree of normal function...
  13. pmc Rab27a is an essential component of melanosome receptor for myosin Va
    Xufeng Wu
    Laboratories of Cell Biology and Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Biol Cell 13:1735-49. 2002
    ....
  14. pmc Myosin-10 produces its power-stroke in two phases and moves processively along a single actin filament under low load
    Yasuharu Takagi
    Laboratory of Molecular Physiology, National Heart, Lung and Blood, Institute, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 111:E1833-42. 2014
    ..We discuss the implications of this load-dependent processivity of myosin-10 as a filopodial transport motor. ..
  15. pmc A FERM domain autoregulates Drosophila myosin 7a activity
    Yi Yang
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:4189-94. 2009
    ..We propose that at low cellular actin concentrations, myosin 7a-FL is bent and inactive, but at high actin concentrations, it is unfolded and active because the C-terminal FERM domain binds to actin...
  16. ncbi request reprint Two-headed binding of the unphosphorylated nonmuscle heavy meromyosin.ADP complex to actin
    Mihaly Kovacs
    Laboratory of Molecular Cardiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    Biochemistry 43:4219-26. 2004
    ..2001) Proc. Natl. Acad. Sci. U.S.A. 98, 4361) is likely to represent an ATPase intermediate that precedes the actomyosin-ADP state...
  17. pmc Drosophila melanogaster myosin-18 represents a highly divergent motor with actin tethering properties
    Stephanie Guzik-Lendrum
    Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 286:21755-66. 2011
    ..Combined, these data suggest that this highly divergent motor may function as an actin tethering protein...
  18. doi request reprint Human myosin Vc is a low duty ratio, nonprocessive molecular motor
    Yasuharu Takagi
    Laboratory of Molecular Physiology, NHLBI, NIH, Bethesda, MD 20892 8015, USA
    J Biol Chem 283:8527-37. 2008
    ..This, in turn, may possibly imply a regulatory mechanism that may be sensitive to moderate changes in ADP concentration...
  19. pmc The B2 alternatively spliced isoform of nonmuscle myosin II-B lacks actin-activated MgATPase activity and in vitro motility
    Kye Young Kim
    Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health Bethesda, MD 20892, USA
    Biochem Biophys Res Commun 369:124-34. 2008
    ..This indicates that the effect of the II-B2 insert is myosin heavy chain specific...
  20. pmc Load-dependent mechanism of nonmuscle myosin 2
    Mihaly Kovacs
    Laboratory of Molecular Physiology, National Heart, Lung, and Blood Institute NIH, Bethesda, MD 20892 8015, USA
    Proc Natl Acad Sci U S A 104:9994-9. 2007
    ..Whereas forward load accelerates the cycle of interaction with actin, resistive load increases duty ratio to favor tension maintenance by two-headed attachment...
  21. ncbi request reprint Calcium and cargoes as regulators of myosin 5a activity
    James R Sellers
    Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, MD 20892 1762, USA
    Biochem Biophys Res Commun 369:176-81. 2008
    ..Thus we propose that the physiologically relevant activation pathway in vivo involves binding of cargo-receptor proteins...
  22. pmc Direct observation of the mechanochemical coupling in myosin Va during processive movement
    Takeshi Sakamoto
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, Bethesda, Maryland 20892, USA
    Nature 455:128-32. 2008
    ..Thus, we directly demonstrate tight coupling between myosin Va movement and the binding and dissociation of nucleotide by simultaneously imaging with near nanometre precision...
  23. pmc Nonmuscle myosin II exerts tension but does not translocate actin in vertebrate cytokinesis
    Xuefei Ma
    Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 109:4509-14. 2012
    ..We propose that the major roles of NM II in vertebrate cell cytokinesis are to bind and cross-link actin filaments and to exert tension on actin during contractile ring constriction...
  24. pmc Kinetic characterization of nonmuscle myosin IIb at the single molecule level
    Attila Nagy
    Laboratory of Molecular Physiology, HLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 288:709-22. 2013
    ..Our results are in good agreement with the previous steady-state and transient kinetic studies and show that the individual nonprocessive nonmuscle myosin IIB molecules form a highly processive unit when polymerized into filaments...
  25. ncbi request reprint Blebbistatin, a myosin II inhibitor, is photoinactivated by blue light
    Takeshi Sakamoto
    Laboratory of Molecular Cardiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    Biochemistry 44:584-8. 2005
    ..This property may be useful in locally reversing the action of blebbistatin treatment in a cell. However, caution should be exercised as free radicals may be produced upon irradiation of blebbistatin that could result in cell damage...
  26. pmc Dimerized Drosophila myosin VIIa: a processive motor
    Yi Yang
    Laboratory of Molecular Physiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1762, USA
    Proc Natl Acad Sci U S A 103:5746-51. 2006
    ..In cells, this kinetic behavior would allow myosin VIIa to exert and hold tension on actin filaments and, if dimerized, to function as a processive cargo transporter...
  27. pmc Kinetic characterization of the ATPase and actin-activated ATPase activities of Acanthamoeba castellanii myosin-2
    Sarah M Heissler
    From the Laboratory of Molecular Physiology and the Laboratory of Cell Biology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 8015
    J Biol Chem 288:26709-20. 2013
    ..As consequence of this change, the duty ratio of this conventional myosin decreases. We speculate on the effect of Ser-639 phosphorylation on the processive behavior of myosin-2 filaments. ..
  28. ncbi request reprint Step-size is determined by neck length in myosin V
    Takeshi Sakamoto
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    Biochemistry 44:16203-10. 2005
    ..These data demonstrate that the step-size of myosin V is affected by the length of its neck and is not solely determined by the pseudo-repeat of the actin filament...
  29. ncbi request reprint Regulated conformation of myosin V
    Fei Wang
    Laboratory of Molecular Cardiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:2333-6. 2004
    ..These data suggest that interaction between the motor and cargo binding domains may be a general mechanism for shutting down motor protein activity and thereby regulating the active movement of vesicles in cells...
  30. ncbi request reprint Mechanism of blebbistatin inhibition of myosin II
    Mihaly Kovacs
    Laboratory of Molecular Cardiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    J Biol Chem 279:35557-63. 2004
    ....
  31. doi request reprint Walking to work: roles for class V myosins as cargo transporters
    John A Hammer
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Rev Mol Cell Biol 13:13-26. 2011
    ....
  32. ncbi request reprint Neck length and processivity of myosin V
    Takeshi Sakamoto
    Laboratory of Molecular Cardiology and Electron Microscopy Core, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    J Biol Chem 278:29201-7. 2003
    ..Its weak processivity was also confirmed by actin landing assays...
  33. ncbi request reprint Specificity of blebbistatin, an inhibitor of myosin II
    John Limouze
    Laboratory of Molecular Cardiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Muscle Res Cell Motil 25:337-41. 2004
    ..The drug potently inhibits Dictyostelium myosin II, but poorly inhibits Acanthamoeba myosin II. Blebbistatin did not inhibit representative myosin superfamily members from classes I, V, and X...
  34. ncbi request reprint Trifluoperazine inhibits the MgATPase activity and in vitro motility of conventional and unconventional myosins
    James R Sellers
    Laboratory of Molecular Cardiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Muscle Res Cell Motil 24:579-85. 2003
    ..The MgATPase of this fragment was inhibited by trifluoperazine over the same range of concentrations as the S1 fragment of myosin...
  35. doi request reprint Myosin 18A coassembles with nonmuscle myosin 2 to form mixed bipolar filaments
    Neil Billington
    Laboratory of Molecular Physiology, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892 8015, USA
    Curr Biol 25:942-8. 2015
    ..Given the numerous, fundamental cellular and developmental roles attributed to NM2, our results have far-reaching biological implications...
  36. ncbi request reprint Kinetic mechanism of non-muscle myosin IIB: functional adaptations for tension generation and maintenance
    Fei Wang
    Laboratory of Molecular Cardiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    J Biol Chem 278:27439-48. 2003
    ..This feature is even more pronounced at slightly elevated ADP levels, and it may be important in carrying out the cellular functions of this isoform working in small filamentous assemblies...
  37. pmc Dynamic exchange of myosin VI on endocytic structures
    Lisa M Bond
    Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 287:38637-46. 2012
    ..Taken together, these FRAP results offer insight into both the basic turnover dynamics and the monomer/dimer nature of myosin VI...
  38. pmc Direct observation of the myosin-Va power stroke and its reversal
    James R Sellers
    Laboratory of Molecular Physiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland USA
    Nat Struct Mol Biol 17:590-5. 2010
    ..Nonlinear response to load leading to coordination or oscillations amongst motors might be relevant for many cellular functions...
  39. doi request reprint Regulation of myosin 5a and myosin 7a
    Verl B Siththanandan
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Biochem Soc Trans 39:1136-41. 2011
    ..We show that, although both myosins have a folded inactive state in which domains in the myosin tail interact with the motor domain, the details of the regulation of these two myosins differ greatly...
  40. pmc Extensibility of the extended tail domain of processive and nonprocessive myosin V molecules
    Attila Nagy
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
    Biophys J 97:3123-31. 2009
    ....
  41. pmc Nonmuscle myosin IIA with a GFP fused to the N-terminus of the regulatory light chain is regulated normally
    Andras Kengyel
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Building 50, Room 3523, Bethesda, MD 20892, USA
    J Muscle Res Cell Motil 31:163-70. 2010
    ..These data indicate that despite some kinetic differences the N-terminal GFP fusion to the regulatory light chain is a reasonable model system for studying myosin function in vivo...
  42. ncbi request reprint Identification and characterization of nonmuscle myosin II-C, a new member of the myosin II family
    Eliahu Golomb
    Laboratory of Molecular Cardiology, NHLBI, National Institute of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:2800-8. 2004
    ..5 + 7.2 microm. HMM II-C1 translocated actin filaments at a rate of 0.05 + 0.011 microm/s in the absence of tropomyosin and at 0.072 + 0.019 microm/s in the presence of tropomyosin in an in vitro motility assay...
  43. pmc Local pulsatile contractions are an intrinsic property of the myosin 2A motor in the cortical cytoskeleton of adherent cells
    Michelle A Baird
    Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    Mol Biol Cell 28:240-251. 2017
    ..Our results thus suggest that pulsatile contractions in the cortical cytoskeleton are an intrinsic property of the NM2A motor that may mediate its role in homeostatic maintenance of tension in the cortical cytoskeleton of adherent cells...
  44. ncbi request reprint Myosin-XVa is required for tip localization of whirlin and differential elongation of hair-cell stereocilia
    Inna A Belyantseva
    Section on Human Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Rockville, MD 20850, USA
    Nat Cell Biol 7:148-56. 2005
    ..The interaction of myosin-XVa and whirlin is therefore a key event in hair-bundle morphogenesis...
  45. ncbi request reprint Identification of an organelle receptor for myosin-Va
    Xufeng S Wu
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Cell Biol 4:271-8. 2002
    ....
  46. ncbi request reprint Utility of genetic screening in hypertrophic cardiomyopathy: prevalence and significance of novel and double (homozygous and heterozygous) beta-myosin mutations
    Saidi A Mohiddin
    Inherited Heart Diseases Section, Cardiology Branch, and Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1650, USA
    Genet Test 7:21-7. 2003
    ....
  47. ncbi request reprint Mutations in human nonmuscle myosin IIA found in patients with May-Hegglin anomaly and Fechtner syndrome result in impaired enzymatic function
    Aihua Hu
    Laboratory of Molecular Cardiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1762, USA
    J Biol Chem 277:46512-7. 2002
    ..Biochemical characterization of the mutant is consistent with this mutant being unable to fully adopt the "on" conformation...
  48. pmc Kinetic Adaptations of Myosins for Their Diverse Cellular Functions
    Sarah M Heissler
    Laboratory of Molecular Physiology, National Heart, Lung, and Blood Institute, National Institutes of Health, 50 South Drive, B50 3523, Bethesda, MD 20892 8015, USA
    Traffic 17:839-59. 2016
    ..This review focuses on kinetic competencies that - together with structural adaptations - result in myosins with unique mechanoenzymatic properties targeted to their diverse cellular functions. ..
  49. pmc Various Themes of Myosin Regulation
    Sarah M Heissler
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, 50 South Drive, B50 3529, Bethesda, MD 20892 8015, USA Electronic address
    J Mol Biol 428:1927-46. 2016
    ..Third, we discuss diverse extramolecular allosteric mechanisms ranging from actin-linked regulatory mechanisms to myosin:cargo interactions. At last, we briefly outline the allosteric regulation of myosins with synthetic compounds. ..
  50. pmc Four things to know about myosin light chains as reporters for non-muscle myosin-2 dynamics in live cells
    Sarah M Heissler
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland
    Cytoskeleton (Hoboken) 72:65-70. 2015
    ....
  51. pmc Kinetic characterization of the sole nonmuscle myosin-2 from the model organism Drosophila melanogaster
    Sarah M Heissler
    Laboratory of Molecular Physiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA and The Cell Adhesion Laboratory, Department of Cancer Biology, The Scripps Research Institute, Jupiter, Florida, USA
    FASEB J 29:1456-66. 2015
    ..3 ± 4.1 µM. Together, these data show that Drosophila nonmuscle myosin-2 is a bona fide molecular motor and establish an important link between switch-2 and blebbistatin sensitivity...
  52. pmc Nanometer localization of single green fluorescent proteins: evidence that myosin V walks hand-over-hand via telemark configuration
    Gregory E Snyder
    Physics Department and Center for Biophysics and Computational Biology, University of Illinois Urbana Champaign, Urbana, Illinois 61801, USA
    Biophys J 87:1776-83. 2004
    ..This kink, or "telemark skier" configuration, may cause strain, which, when released, leads to the powerstroke of myosin, throwing the rear head forward and leading to unidirectional motion...
  53. ncbi request reprint The gated gait of the processive molecular motor, myosin V
    Claudia Veigel
    Department of Biology, University of York, PO Box 373, York YO10 5YW, UK
    Nat Cell Biol 4:59-65. 2002
    ..This mechanism increases processivity and enables a single myosin V molecule to travel distances of several hundred nanometres along the actin filament...
  54. pmc The prepower stroke conformation of myosin V
    Stan Burgess
    Astbury Centre for Structural Molecular Biology and School of Biomedical Sciences, University of Leeds, Leeds, UK
    J Cell Biol 159:983-91. 2002
    ..The microscopy shows that most of the walking stride is produced by changes in lever angle brought about by converter movement, but is augmented by distortion produced by thermal energy...
  55. ncbi request reprint Smooth muscle myosin filament assembly under control of a kinase-related protein (KRP) and caldesmon
    Dmitry S Kudryashov
    Laboratory of Cell Motility, Cardiology Research Center, 3rd Cherepkovskaya Street 15A, Moscow 121552, Russia
    J Muscle Res Cell Motil 23:341-51. 2002
    ..We propose a new model that through their independent binding to myosin and differential effects on myosin dynamics, caldesmon and KRP can, in concert, control the length and polymerization state of myosin filaments...
  56. ncbi request reprint The predicted coiled-coil domain of myosin 10 forms a novel elongated domain that lengthens the head
    Peter J Knight
    School of Biomedical Sciences, Astbury Centre for Structural Molecular Biology, and School of Biochemistry and Microbiology, University of Leeds, Leeds, LS2 9JT, United Kingdom
    J Biol Chem 280:34702-8. 2005
    ..Sequence comparisons show that similar structures may exist in the predicted coiled-coil domains of myosins 6 and 7a and MyoM and could function to increase the size of the working stroke...
  57. ncbi request reprint Load-dependent kinetics of myosin-V can explain its high processivity
    Claudia Veigel
    Physical Biochemistry, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nat Cell Biol 7:861-9. 2005
    ....
  58. pmc The cargo-binding domain regulates structure and activity of myosin 5
    Kavitha Thirumurugan
    Institute of Molecular and Cellular Biology, and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    Nature 442:212-5. 2006
    ....
  59. ncbi request reprint Kinetic mechanism of myosinV-S1 using a new fluorescent ATP analogue
    Eva Forgacs
    Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia 23507, USA
    Biochemistry 45:13035-45. 2006
    ..Deac-aminoATP supports motility by myosinV-HMM on actin at a rate consistent with the slower rate of deac-aminoADP dissociation...
  60. pmc Loop 2 of limulus myosin III is phosphorylated by protein kinase A and autophosphorylation
    Karen Kempler
    Whitney Laboratory for Marine Bioscience and the Department of Neuroscience, University of Florida, St Augustine, Florida 32080, USA
    Biochemistry 46:4280-93. 2007
    ....
  61. pmc Diversity of structural behavior in vertebrate conventional myosins complexed with actin
    Hiroyuki Iwamoto
    Research and Utilization Division, SPring 8, Japan Synchrotron Radiation Research Institute, Hyogo 679 6198, Japan
    J Mol Biol 369:249-64. 2007
    ..The results indicate that the "conventional" myosin-II isoforms exhibit a wide variety of structural behavior, possibly depending on their functions and/or the history of molecular evolution...
  62. pmc Myo4p is a monomeric myosin with motility uniquely adapted to transport mRNA
    Brian D Dunn
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 178:1193-206. 2007
    ..Our results suggest that the oligomeric state of Myo4p is important for its motility and ability to transport mRNA...
  63. ncbi request reprint Kinetics of ADP dissociation from the trail and lead heads of actomyosin V following the power stroke
    Eva Forgacs
    Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia 23507, USA
    J Biol Chem 283:766-73. 2008
    ..The dissociation of ADP from the lead head is decreased by up to 250-fold...
  64. pmc The small molecule tool (S)-(-)-blebbistatin: novel insights of relevance to myosin inhibitor design
    Cristina Lucas-Lopez
    School of Chemistry and the Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife, UK
    Org Biomol Chem 6:2076-84. 2008
    ..These studies are ultimately directed at the development of novel myosin inhibitors with improved affinity and different selectivity profiles from blebbistatin itself...
  65. ncbi request reprint Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor
    Aaron F Straight
    Department of Cell Biology and Institute of Chemistry and Cell Biology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA
    Science 299:1743-7. 2003
    ..Continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components...
  66. ncbi request reprint Drosophila myosin V is required for larval development and spermatid individualization
    Valerie Mermall
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06510, USA
    Dev Biol 286:238-55. 2005
    ..Our results suggest that MyoV contributes to the formation of the actin-based investment cones and acts to coordinate and/or anchor these structures and other components of the individualization complex...