Genomes and Genes
Affiliation: National Institutes of Health
- Construction of FLAG and histidine tagging vectors for Schizosaccharomyces pombeY Huang
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development NIH, Building 6, Room 3403, 6 Center Drive MSC 2753, Bethesda, MD 20892 2753, USA
Yeast 18:463-8. 2001..The function and applicability of these vectors were examined and the results are shown using the N-terminal tagging vector encoding Sfc6p, a subunit of the Sz. pombe RNA polymerase III general transcription factor, TFIIIC...
- Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombeYing Huang
Nucleic Acids Res 30:1465-82. 2002..cerevisiae. Silencing proteins from both yeasts are also actively involved in other chromosome-related nuclear functions, including DNA repair and the regulation of chromatin structure...
- Hepatic gene expression during treatment with peginterferon and ribavirin: Identifying molecular pathways for treatment responseJordan J Feld
Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
Hepatology 46:1548-63. 2007..Collectively, these mechanisms may provide a molecular basis for the improved efficacy of combination therapy...
- Barrier-to-autointegration factor (BAF) condenses DNA by loopingDunja Skoko
Laboratory of Molecular Biology, Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 106:16610-5. 2009..Thus the DNA binding properties of BAF may alone be sufficient to account for its association with the preintegration complex...
- Structural basis for dimerization of LAP2alpha, a component of the nuclear laminaChristina Marchetti Bradley
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Structure 15:643-53. 2007..The surface involved in binding lamin A/C is proposed based on results from alanine-scanning mutagenesis and a solid-phase overlay binding assay...
- Separate RNA-binding surfaces on the multifunctional La protein mediate distinguishable activities in tRNA maturationYing Huang
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, US National Institutes of Health, 31 Center Dr, Rm 2A25, Bethesda, Maryland 20892, USA
Nat Struct Mol Biol 13:611-8. 2006..The two activities of La in tRNA maturation map to its two conserved RNA-binding surfaces and suggest a modular model that has implications for its other ligands...
- Folliculin (Flcn) inactivation leads to murine cardiac hypertrophy through mTORC1 deregulationYukiko Hasumi
Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Hum Mol Genet 23:5706-19. 2014..These data support an important role for Flcn in cardiac homeostasis in the murine model. ..
- RNA polymerase III from the fission yeast, Schizosaccharomyces pombeYing Huang
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
Methods Enzymol 370:165-73. 2003
- Mutations in the RNA polymerase III subunit Rpc11p that decrease RNA 3' cleavage activity increase 3'-terminal oligo(U) length and La-dependent tRNA processingYing Huang
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, NIH, 31 Center Dr, Room 2A25, Bethesda, MD 20892 2426, USA
Mol Cell Biol 25:621-36. 2005..The data indicate that Rpc11p limits RNA 3'-U length and that this significantly restricts pre-tRNAs to a La-independent pathway of maturation in fission yeast...
- Solution NMR structure of the barrier-to-autointegration factor-Emerin complexMengli Cai
Laboratories of Chemical Physics and Molecular Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892 0520, USA
J Biol Chem 282:14525-35. 2007..The relevance of the implications of the structural and biophysical data on the complex in the context of the interaction between the BAF(2) dimer and Em(LEM) at the nuclear envelope is discussed...
- Human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 use different receptor complexes to enter T cellsKathryn S Jones
Basic Research Program, SAIC Frederick, Inc, National Cancer Institute Frederick, Maryland 21702, USA
J Virol 80:8291-302. 2006....
- A simple and robust protocol for high-yield expression of perdeuterated proteins in Escherichia coli grown in shaker flasksMengli Cai
Laboratories of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892 0520, USA
J Biomol NMR 66:85-91. 2016..The method increases the yield of perdeuterated proteins by up to tenfold using commonly available air shakers without any requirement for specialized fermentation equipment...
- Regulation of mitochondrial oxidative metabolism by tumor suppressor FLCNHisashi Hasumi
Urologic Oncology Branch, National Cancer Institute, 10 Center Dr, MSC 1107, CRC Rm 1 5940W, Bethesda, MD 20892, USA
J Natl Cancer Inst 104:1750-64. 2012..Genetic studies of BHD patients have uncovered the causative gene, FLCN, but its function is incompletely understood...
- Phosphorylation of hepatitis C virus NS5A nonstructural protein: a new paradigm for phosphorylation-dependent viral RNA replication?Ying Huang
Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Virology 364:1-9. 2007..Understanding the process of NS5A phosphorylation and the definite identification of the culprit cellular protein kinase(s) will shed light on the mechanisms of HCV RNA replication and/or pathogenesis...
- Equilibrium interactions of corepressors and coactivators with agonist and antagonist complexes of glucocorticoid receptorsQi Wang
Steriod Hormones Section, National Institute of Diabetes and Digestive and Kidney Disease Laboratory of Molecular and Cellular Biology, National Institutes of Health, Bethesda, Maryland 20892, USA
Mol Endocrinol 18:1376-95. 2004..This modulation provides an attractive mechanism for differential control of gene expression during development, differentiation, homeostasis, and endocrine therapies...
- Defective hepatic response to interferon and activation of suppressor of cytokine signaling 3 in chronic hepatitis CYing Huang
Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Gastroenterology 132:733-44. 2007..To understand IFN-alpha resistance in vivo, we examined the dynamic responses to both type I and type II IFNs, human IFN (hIFN)-alpha, -gamma, and consensus IFN, in the chimpanzee model...
- The fission yeast TFIIB-related factor limits RNA polymerase III to a TATA-dependent pathway of TBP recruitmentYing Huang
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2753, USA
Nucleic Acids Res 31:2108-16. 2003..Since previous whole genome analysis also revealed TATA elements upstream of tRNA genes in Arabidopsis, this pathway may be more widespread than appreciated previously...
- Induction of human T cell leukemia virus type I receptors on quiescent naive T lymphocytes by TGF-betaKathryn S Jones
Basic Research Program, Science Applications International Corporation Frederick, Inc, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
J Immunol 174:4262-70. 2005....
- The Hsp40 family chaperone protein DnaJB6 enhances Schlafen1 nuclear localization which is critical for promotion of cell-cycle arrest in T-cellsYafeng Zhang
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, People s Republic of China
Biochem J 413:239-50. 2008..Altogether, our results demonstrate that DnaJB6 is necessary for translocation of Slfn1 into the nucleus, where Slfn1 down-regulates cyclin D1, induces cell-cycle arrest and programmes a quiescent state of T-cells...
- Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinomaYing Huang
Human Cancer Genetics Program, Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA
Int J Cancer 104:735-44. 2003..In contrast, hypermethylation of promoter CpG islands seems to occur at higher frequency. MT1G and CRABP1 are novel genes that are likely involved in the pathogenesis of sporadic PTC...
- Novel poly(ethylene imine) biscarbamate conjugate as an efficient and nontoxic gene delivery systemSonglin Xu
School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China
J Control Release 130:64-8. 2008..PEIC can also transfect MeWo cells with large genes such as the 125kb Varicella-Zoster viral gene (VZV) at high activity...
- Structured to reduce the mitogenicity of anti-CD3 antibody based on computer-guided molecular designMing Lv
Institute of Basic Medical Sciences, Beijing, PR China
Int J Biochem Cell Biol 39:1142-55. 2007....
- Similar regulation of cell surface human T-cell leukemia virus type 1 (HTLV-1) surface binding proteins in cells highly and poorly transduced by HTLV-1-pseudotyped virionsKathryn S Jones
Basic Research Program, SAIC Frederick, National Cancer Institute at Frederick, Frederick, MD 21702 1201, USA
J Virol 76:12723-34. 2002..These results suggest that factors other than the number of primary binding receptors are responsible for the differences in the titers of HTLV-1 pseudotypes between highly susceptible cells and poorly susceptible cells...
- TC21 mediates transformation and cell survival via activation of phosphatidylinositol 3-kinase/Akt and NF-kappaB signaling pathwayRong Rong
Department of Pharmacology, State University of New York, Upstate Medical University, 750 E Adams Street, Syracuse, NY 13210, USA
Oncogene 21:1062-70. 2002..In conclusion, we have identified PI3-K as an effector of TC21 and demonstrated that the PI3-K/Akt signaling pathway plays important roles in TC21-mediated transformation and cell survival...
- Retinoic acid-induced AP-1 transcriptional activity regulates B16 mouse melanoma growth inhibition and differentiationYing Huang
Department of Biochemistry and Molecular Biology, Joan C Edwards School of Medicine, Marshall University, Huntington, West Virginia 25704, USA
J Cell Physiol 194:162-70. 2003..Our observations suggest that AP-1 transcriptional activity induced by RA likely plays an important role in the biological changes mediated by this retinoid in B16 melanoma cells...
- An efficient and targeted gene integration system for high-level antibody expressionYing Huang
Department of Molecular Immunology, Beijing Institute of Basic Medical Sciences, Beijing 100850, PR China
J Immunol Methods 322:28-39. 2007..Three kinds of recombinant antibodies were successfully expressed in candidate cell line 37. The highest producers produced more than 200 mug/ml of the antibody in 6 days of continuous culture in a spinner flask...
- Receptor isoform and ligand-specific modulation of dihydrotestosterone-induced prostate specific antigen gene expression and prostate tumor cell growth by estrogensYuan Shan Zhu
Department of Medicine Endocrinology, Weill Medical College of Cornell University, 1300 York Ave, Box 149, New York, NY 10021, USA
J Androl 26:500-8; discussion 509-10. 2005....
- Heparan sulfate proteoglycans mediate attachment and entry of human T-cell leukemia virus type 1 virions into CD4+ T cellsKathryn S Jones
Basic Research Program, SAIC Frederick, National Cancer Institute Frederick, MD 21702 1201, USA
J Virol 79:12692-702. 2005..Thus, HSPGs play a critical role in the binding and entry of HTLV-1 into CD4(+) T cells...
- Interferon-alpha-induced expression of phospholipid scramblase 1 through STAT1 requires the sequential activation of protein kinase Cdelta and JNKKe Wen Zhao
Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis, Chinese Ministry of Education, Rui Jin Hospital, Shanghai Jiaotong University School of Medicine formerly Shanghai Second Medical University, Shanghai 200025, China
J Biol Chem 280:42707-14. 2005..In conclusion, our results suggest that the induction of PLSCR1 transcription through STAT1 depends upon sequential activation of PKCdelta and JNK...
- [A method of HPRE synthesis via transcription by T7 RNA polymerase in vitro]Ying Huang
Key Laboratory of Infectious Diseases, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400016, China
Zhonghua Gan Zang Bing Za Zhi 13:808-10. 2005..To synthesize highly pure HBV post-transcriptional regulatory element (HPRE) via transcription in vitro by T7 RNA polymerase...
- Alteration of protein subcellular location and domain formation by alternative translational initiationJun Cai
Institute of Bioinformatics, MOE Key Laboratory of Bioinformatics, Department of Automation, Tsinghua University, Beijing, China
Proteins 62:793-9. 2006..The peculiar consequence of subcellular relocation as well as change of domain contents reflected the very high level of biological complexity as alternative usage of initiation codons...
- Cyclooxygenase-2 interacts with p53 and interferes with p53-dependent transcription and apoptosisChad A Corcoran
Department of Pharmacology, State University of New York, Upstate Medical University, Syracuse, NY 13210, USA
Oncogene 24:1634-40. 2005..Based on our results, we propose that p53-dependent upregulation and activation of COX-2 appear to be yet another novel mechanism by which p53 could abate its own growth-inhibitory and apoptotic effects...
- Calcium-sensing receptor ubiquitination and degradation mediated by the E3 ubiquitin ligase dorfinYing Huang
Department of Biology, Syracuse University, Syracuse, New York 13244, USA
J Biol Chem 281:11610-7. 2006..Because endogenous CaR in Madin-Darby canine kidney cells is also subject to degradation from the endoplasmic reticulum, dorfin-mediated ubiquitination may contribute to a general mechanism for CaR quality control during biosynthesis...
- Cloning and characterization of a novel gene PDRG that is differentially regulated by p53 and ultraviolet radiationXiuquan Luo
Department of Pharmacology, State University of New York, Upstate Medical University, Syracuse, NY 12310, USA
Oncogene 22:7247-57. 2003..Furthermore, stable expression of exogenous PDRG was found to decrease the clonogenic survival after UV irradiation, which highlights the significance of PDRG in facilitating UV-induced killing...
- Binding activity difference of anti-CD20 scFv-Fc fusion protein derived from variable domain exchangeShu Sheng Geng
College of Life Science, Hebei University, Baoding, Hebei, China
Cell Mol Immunol 3:439-43. 2006..The study highlights the potential relationship between the antibody binding activity and their 3-D conformation, which appears to be worthwhile in providing direction for future antibody design of recombinant antibody...
- Regulation of recombinant monoclonal antibody production in chinese hamster ovary cells: a comparative study of gene copy number, mRNA level, and protein expressionZhou Jiang
The Isermann Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
Biotechnol Prog 22:313-8. 2006..We also observed that the HC to LC gene copy ratio may affect the protein yield in MAb expression systems...