Robert S Fischer

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Thematic Minireview Series: The State of the Cytoskeleton in 2015
    Robert S Fischer
    From the NHLBI, National Institutes of Health, Bethesda, Maryland 20892 and
    J Biol Chem 290:17133-6. 2015
  2. pmc Move your microvilli
    Robert S Fischer
    Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda MD, 20892
    J Cell Biol 207:9-11. 2014
  3. pmc Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior
    Robert S Fischer
    Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute NHBLI, US National Institutes of Health NIH, Bethesda, Maryland, USA
    Nat Protoc 7:2056-66. 2012
  4. pmc Microscopy in 3D: a biologist's toolbox
    Robert S Fischer
    National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Cell Biol 21:682-91. 2011
  5. pmc Distinct ECM mechanosensing pathways regulate microtubule dynamics to control endothelial cell branching morphogenesis
    Kenneth A Myers
    Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 192:321-34. 2011
  6. pmc Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy
    Yicong Wu
    Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
    Nat Biotechnol 31:1032-8. 2013
  7. pmc Local pulsatile contractions are an intrinsic property of the myosin 2A motor in the cortical cytoskeleton of adherent cells
    Michelle A Baird
    Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    Mol Biol Cell 28:240-251. 2017
  8. pmc FMN2 Makes Perinuclear Actin to Protect Nuclei during Confined Migration and Promote Metastasis
    Colleen T Skau
    Cell Biology and Physiology Center, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 167:1571-1585.e18. 2016
  9. pmc Rac1-dependent phosphorylation and focal adhesion recruitment of myosin IIA regulates migration and mechanosensing
    Ana M Pasapera
    Cell Biology and Physiology Center, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Biol 25:175-86. 2015
  10. pmc A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells
    Dylan T Burnette
    National Institute of Child Health and Human Development and 2 National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    J Cell Biol 205:83-96. 2014

Collaborators

Detail Information

Publications14

  1. pmc Thematic Minireview Series: The State of the Cytoskeleton in 2015
    Robert S Fischer
    From the NHLBI, National Institutes of Health, Bethesda, Maryland 20892 and
    J Biol Chem 290:17133-6. 2015
    ..In this thematic series of minireviews, these topics are covered by some of the very same scientists who generated these recent insights, thereby providing us with an overview of the State of the Cytoskeleton in 2015. ..
  2. pmc Move your microvilli
    Robert S Fischer
    Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda MD, 20892
    J Cell Biol 207:9-11. 2014
    ..Their unexpected observations may have implications for epithelial responses in wound healing and disease. ..
  3. pmc Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior
    Robert S Fischer
    Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute NHBLI, US National Institutes of Health NIH, Bethesda, Maryland, USA
    Nat Protoc 7:2056-66. 2012
    ..This type of system enables high-resolution time-lapse imaging and is suitable for a wide range of cell types and molecular perturbations...
  4. pmc Microscopy in 3D: a biologist's toolbox
    Robert S Fischer
    National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Cell Biol 21:682-91. 2011
    ..We discuss a few specific microscopy modalities that we believe will be particularly advantageous in imaging cells and subcellular structures in physiologically relevant 3D environments...
  5. pmc Distinct ECM mechanosensing pathways regulate microtubule dynamics to control endothelial cell branching morphogenesis
    Kenneth A Myers
    Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 192:321-34. 2011
    ..Thus, compliance and dimensionality ECM mechanosensing pathways independently regulate specific and distinct microtubule dynamics parameters in endothelial cells to guide branching morphogenesis in physically complex ECMs...
  6. pmc Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy
    Yicong Wu
    Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
    Nat Biotechnol 31:1032-8. 2013
    ....
  7. pmc Local pulsatile contractions are an intrinsic property of the myosin 2A motor in the cortical cytoskeleton of adherent cells
    Michelle A Baird
    Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    Mol Biol Cell 28:240-251. 2017
    ..Our results thus suggest that pulsatile contractions in the cortical cytoskeleton are an intrinsic property of the NM2A motor that may mediate its role in homeostatic maintenance of tension in the cortical cytoskeleton of adherent cells...
  8. pmc FMN2 Makes Perinuclear Actin to Protect Nuclei during Confined Migration and Promote Metastasis
    Colleen T Skau
    Cell Biology and Physiology Center, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cell 167:1571-1585.e18. 2016
    ..Our results indicate a critical role for FMN2 in generating a perinuclear actin/FA system that protects the nucleus and DNA from damage to promote cell survival during confined migration and thus promote cancer metastasis...
  9. pmc Rac1-dependent phosphorylation and focal adhesion recruitment of myosin IIA regulates migration and mechanosensing
    Ana M Pasapera
    Cell Biology and Physiology Center, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Biol 25:175-86. 2015
    ..Signaling downstream of the small GTPase Rac1 also regulates FA and actin dynamics, but its role in regulation of MII during migration is less clear...
  10. pmc A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells
    Dylan T Burnette
    National Institute of Child Health and Human Development and 2 National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892
    J Cell Biol 205:83-96. 2014
    ....
  11. pmc Instant super-resolution imaging in live cells and embryos via analog image processing
    Andrew G York
    Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
    Nat Methods 10:1122-6. 2013
    ..We demonstrate the capability of our system by imaging fine, rapidly moving structures including motor-driven organelles in human lung fibroblasts and the cytoskeleton of flowing blood cells within developing zebrafish embryos. ..
  12. pmc Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy
    Andrew G York
    Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, US National Institutes of Health NIH, Bethesda, Maryland, USA
    Nat Methods 9:749-54. 2012
    ..Our method uses commercially available parts and open-source software and is simpler than existing SIM implementations, allowing easy integration with wide-field microscopes...
  13. pmc YAP Nuclear Localization in the Absence of Cell-Cell Contact Is Mediated by a Filamentous Actin-dependent, Myosin II- and Phospho-YAP-independent Pathway during Extracellular Matrix Mechanosensing
    Arupratan Das
    From the Cell Biology and Physiology Center, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 and the Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    J Biol Chem 291:6096-110. 2016
    ..We suggest that in non-contact inhibited cells, this latter mechanism may be important in low stiffness regimes, such as may be encountered in physiological environments. ..
  14. pmc Local cortical tension by myosin II guides 3D endothelial cell branching
    Robert S Fischer
    Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Biol 19:260-5. 2009
    ..Thus, local downregulation of myosin II cortical contraction allows pseudopodium initiation to mediate EC branching and hence guide directional migration and angiogenesis...