Xin Chen

Summary

Affiliation: National Institutes of Health
Country: USA

Publications

  1. pmc Th17 cells and Tregs: unlikely allies
    Xin Chen
    Basic Science Program, Leidos Biomedical Research, Inc, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA and
    J Leukoc Biol 95:723-731. 2014
  2. pmc TNFR2 is critical for the stabilization of the CD4+Foxp3+ regulatory T. cell phenotype in the inflammatory environment
    Xin Chen
    Basic Science Program, Science Applications International Corporation Frederick, National Cancer Institute Frederick, Frederick, MD 21702, USA
    J Immunol 190:1076-84. 2013
  3. pmc The phenotypic and functional consequences of tumour necrosis factor receptor type 2 expression on CD4(+) FoxP3(+) regulatory T cells
    Xin Chen
    Basic Science Program, SAIC Frederick, Inc, Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center for Cancer Research, NCI Frederick, Frederick, MD, USA
    Immunology 133:426-33. 2011
  4. pmc Cutting edge: expression of TNFR2 defines a maximally suppressive subset of mouse CD4+CD25+FoxP3+ T regulatory cells: applicability to tumor-infiltrating T regulatory cells
    Xin Chen
    Basic Research Program, SAIC Frederick Inc, National Cancer Institute NCI Frederick, Frederick, MD 21702 1201, USA
    J Immunol 180:6467-71. 2008
  5. pmc Resolving the identity myth: key markers of functional CD4+FoxP3+ regulatory T cells
    Xin Chen
    Basic Science Program, SAIC Frederick, Inc, Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, NCI Frederick, Frederick, Maryland 21702, United States
    Int Immunopharmacol 11:1489-96. 2011
  6. ncbi request reprint Dendritic cells as a pharmacological target of traditional Chinese medicine
    Xin Chen
    Basic Research Program, SAIC Frederick, Inc, Lab of Molecular Immunoregulation, Cancer and Inflammation Program, CCR, NCI Frederick, P O Box B, Bldg 560, Rm 31 19, Maryland 21702 1201, USA
    Cell Mol Immunol 3:401-10. 2006
  7. doi request reprint Expression of costimulatory TNFR2 induces resistance of CD4+FoxP3- conventional T cells to suppression by CD4+FoxP3+ regulatory T cells
    Xin Chen
    Basic Science Program, Science Applications International Corporation Frederick, Inc, National Cancer Institute, Frederick, MD 21702, USA
    J Immunol 185:174-82. 2010
  8. doi request reprint TNF-alpha: an activator of CD4+FoxP3+TNFR2+ regulatory T cells
    Xin Chen
    Basic Science Program, SAIC Frederick, Inc, National Cancer Institute Frederick, MD 21702, USA
    Curr Dir Autoimmun 11:119-34. 2010
  9. ncbi request reprint Pertussis toxin by inducing IL-6 promotes the generation of IL-17-producing CD4 cells
    Xin Chen
    Basic Research Program, SAIC Frederick, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
    J Immunol 178:6123-9. 2007
  10. ncbi request reprint Interaction of TNF with TNF receptor type 2 promotes expansion and function of mouse CD4+CD25+ T regulatory cells
    Xin Chen
    Laboratory of Molecular Immunoregulation, Basic Research Program, SAIC Frederick, National Cancer Institute, Frederick, MD 21702, USA
    J Immunol 179:154-61. 2007

Collaborators

Detail Information

Publications84

  1. pmc Th17 cells and Tregs: unlikely allies
    Xin Chen
    Basic Science Program, Leidos Biomedical Research, Inc, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA and
    J Leukoc Biol 95:723-731. 2014
    ..This bidirectional crosstalk is largely dependent on the TNF-TNFR2 pathway. These mutual stimulatory effects should be considered in devising future Th17 cell- and Treg-targeting therapy...
  2. pmc TNFR2 is critical for the stabilization of the CD4+Foxp3+ regulatory T. cell phenotype in the inflammatory environment
    Xin Chen
    Basic Science Program, Science Applications International Corporation Frederick, National Cancer Institute Frederick, Frederick, MD 21702, USA
    J Immunol 190:1076-84. 2013
    ..This effect of TNF should be taken into account when designing future therapy of autoimmunity and graft-versus-host disease by using TNF inhibitors...
  3. pmc The phenotypic and functional consequences of tumour necrosis factor receptor type 2 expression on CD4(+) FoxP3(+) regulatory T cells
    Xin Chen
    Basic Science Program, SAIC Frederick, Inc, Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center for Cancer Research, NCI Frederick, Frederick, MD, USA
    Immunology 133:426-33. 2011
    ..The contribution of TNFR2 expression on Treg and Teff cells to the beneficial and detrimental effects of anti-TNF treatment in autoimmune disorders will also be discussed...
  4. pmc Cutting edge: expression of TNFR2 defines a maximally suppressive subset of mouse CD4+CD25+FoxP3+ T regulatory cells: applicability to tumor-infiltrating T regulatory cells
    Xin Chen
    Basic Research Program, SAIC Frederick Inc, National Cancer Institute NCI Frederick, Frederick, MD 21702 1201, USA
    J Immunol 180:6467-71. 2008
    ..Thus, TNFR2 identifies a unique subset of mouse Tregs with an activated/memory phenotype and maximal suppressive activity that may account for tumor-infiltrating lymphocyte-mediated immune evasion by tumors...
  5. pmc Resolving the identity myth: key markers of functional CD4+FoxP3+ regulatory T cells
    Xin Chen
    Basic Science Program, SAIC Frederick, Inc, Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, NCI Frederick, Frederick, Maryland 21702, United States
    Int Immunopharmacol 11:1489-96. 2011
    ....
  6. ncbi request reprint Dendritic cells as a pharmacological target of traditional Chinese medicine
    Xin Chen
    Basic Research Program, SAIC Frederick, Inc, Lab of Molecular Immunoregulation, Cancer and Inflammation Program, CCR, NCI Frederick, P O Box B, Bldg 560, Rm 31 19, Maryland 21702 1201, USA
    Cell Mol Immunol 3:401-10. 2006
    ..These studies have revealed novel biological effects of TCM and documented the utility of this approach to discover novel biological modifier of DC functions derived from natural sources...
  7. doi request reprint Expression of costimulatory TNFR2 induces resistance of CD4+FoxP3- conventional T cells to suppression by CD4+FoxP3+ regulatory T cells
    Xin Chen
    Basic Science Program, Science Applications International Corporation Frederick, Inc, National Cancer Institute, Frederick, MD 21702, USA
    J Immunol 185:174-82. 2010
    ..However, intratumoral Tregs expressing more TNFR2 are able to overcome the greater resistance to suppression of intratumoral Tconv cells, resulting in a dominant immunosuppressive tumor environment...
  8. doi request reprint TNF-alpha: an activator of CD4+FoxP3+TNFR2+ regulatory T cells
    Xin Chen
    Basic Science Program, SAIC Frederick, Inc, National Cancer Institute Frederick, MD 21702, USA
    Curr Dir Autoimmun 11:119-34. 2010
    ..The role of Tregs in the autoimmune disorders and cancer as well as the effect of anti-TNF therapy on Tregs, especially in rheumatoid arthritis, will also be considered...
  9. ncbi request reprint Pertussis toxin by inducing IL-6 promotes the generation of IL-17-producing CD4 cells
    Xin Chen
    Basic Research Program, SAIC Frederick, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
    J Immunol 178:6123-9. 2007
    ..Thus, in addition to inhibiting the development of Treg, the immunoadjuvant activity of PTx can be attributable to the generation of IL-6-dependent IL-17-producing CD4 cells...
  10. ncbi request reprint Interaction of TNF with TNF receptor type 2 promotes expansion and function of mouse CD4+CD25+ T regulatory cells
    Xin Chen
    Laboratory of Molecular Immunoregulation, Basic Research Program, SAIC Frederick, National Cancer Institute, Frederick, MD 21702, USA
    J Immunol 179:154-61. 2007
    ..Moreover, our study suggests that the slower response of Tregs than Teffs to TNF results in delayed immunosuppressive feedback effects...
  11. pmc Co-expression of TNFR2 and CD25 identifies more of the functional CD4+FOXP3+ regulatory T cells in human peripheral blood
    Xin Chen
    Basic Science Program, Laboratory of Molecular Immunoregulation, SAIC Frederick, Inc, NCI Frederick, Frederick, MD 21702 1201, USA
    Eur J Immunol 40:1099-106. 2010
    ..As some non-Treg express TNFR2, the combination of CD25 and TNFR2 must be used to identify a larger population of human Treg, a population that may prove to be of diagnostic and therapeutic benefit in cancer and autoimmune diseases...
  12. pmc Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking
    Xin Chen
    Basic Research Program, Science Applications International Corporation Frederick, Inc, Laboratory of Molecular Immunoregulation, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
    Blood 106:2409-16. 2005
    ..These data provide new insight into the mechanism of action of TPT and indicate that the inhibition of maturation and trafficking of DCs by TPT contributes to its immunosuppressive effects...
  13. ncbi request reprint Yin zi huang, an injectable multicomponent chinese herbal medicine, is a potent inhibitor of T-cell activation
    Xin Chen
    Basic Research Program, SAIC Frederick, Inc, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
    J Altern Complement Med 10:519-26. 2004
    ..The effects of 10 ingredient components of YZH were also evaluated...
  14. ncbi request reprint BALB/c mice have more CD4+CD25+ T regulatory cells and show greater susceptibility to suppression of their CD4+CD25- responder T cells than C57BL/6 mice
    Xin Chen
    Basic Research Program, SAIC Frederick, Inc, MD 21702, USA
    J Leukoc Biol 78:114-21. 2005
    ..Our data also suggest that differences in the CD4(+)CD25(+) Treg cell number and the susceptibility of CD4(+)CD25(-) Tres cells may, at least in part, account for the differences in immune response between B6 and BALB/c strains of mice...
  15. ncbi request reprint Triptolide attenuates endotoxin- and staphylococcal exotoxin-induced T-cell proliferation and production of cytokines and chemokines
    Teresa Krakauer
    Department of Immunology and Molecular Biology, United States Army Medical Research Institute of Infectious Diseases, Bldg 1425, Fort Detrick, Frederick, MD 21702 5011, USA
    Immunopharmacol Immunotoxicol 27:53-66. 2005
    ..It also may be therapeutically useful for mitigating the pathogenic effects of these microbial products by downregulating the signaling pathways activated by both bacterial exotoxins and endotoxins...
  16. pmc Successful immunotherapy with IL-2/anti-CD40 induces the chemokine-mediated mitigation of an immunosuppressive tumor microenvironment
    Jonathan M Weiss
    Laboratory of Experimental Immunology, Cancer and Inflammation Program, NCI Frederick, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 106:19455-60. 2009
    ..These results suggest that the use of anti-CD40 in combination with IL-2 or IL-15 may hold substantially more promise for clinical cancer treatment than anti-CD40 alone...
  17. ncbi request reprint Differential response of murine CD4+CD25+ and CD4+CD25- T cells to dexamethasone-induced cell death
    Xin Chen
    Basic Research Program, SAIC Frederick, Inc, Frederick, MD, USA
    Eur J Immunol 34:859-69. 2004
    ..Our data also suggest that selective protection of CD4+CD25+ T cell from apoptosis may constitute a role in immune tolerance for IL-2...
  18. pmc Pertussis toxin as an adjuvant suppresses the number and function of CD4+CD25+ T regulatory cells
    Xin Chen
    Basic Research Program, SAIC Frederick, Inc, Laboratory of Molecular Immunoregulation, National Cancer Institute Frederick Frederick, MD 21702 1201, USA
    Eur J Immunol 36:671-80. 2006
    ..This study concludes that an underlying cause for the immunological adjuvanticity of PTx is down-regulation of Treg cell number and function...
  19. ncbi request reprint Functional and genomic analyses of FOXP3-transduced Jurkat-T cells as regulatory T (Treg)-like cells
    Joon Young Kim
    Cancer Stem Cell Section, Laboratory of Cancer Prevention, NCI Frederick, 1050 Boyles Street, Building 560, Room 21 78, Frederick, MD 21702, USA
    Biochem Biophys Res Commun 362:44-50. 2007
    ....
  20. ncbi request reprint Glucocorticoid amplifies IL-2-dependent expansion of functional FoxP3(+)CD4(+)CD25(+) T regulatory cells in vivo and enhances their capacity to suppress EAE
    Xin Chen
    Basic Research Program, SAIC Frederick, Center for Cancer Research, National Cancer Institute Frederick, National Institutes of Health, Frederick, MD 21702, USA
    Eur J Immunol 36:2139-49. 2006
    ..Therefore, the combination of glucocorticoid and IL-2, two currently used therapeutics, may provide a novel approach for the treatment of autoimmune diseases, transplant rejection and graft-vs.-host disease...
  21. ncbi request reprint Tannic acid is an inhibitor of CXCL12 (SDF-1alpha)/CXCR4 with antiangiogenic activity
    Xin Chen
    The Basic Research Program, Science Applications International Corporation Frederick, Inc Gaithersburg, Maryland 20879, USA
    Clin Cancer Res 9:3115-23. 2003
    ..The antiangiogenic effect of tannic acid was assessed by in vitro endothelial cell tube formation...
  22. pmc Development of regulatory T cells requires IL-7Ralpha stimulation by IL-7 or TSLP
    Renata Mazzucchelli
    Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute NCI, National Institutes of Health NIH, Frederick, MD, USA
    Blood 112:3283-92. 2008
    ....
  23. ncbi request reprint RNase protection assay for the study of the differential effects of therapeutic agents in suppressing staphylococcal enterotoxin B-induced cytokines in human peripheral blood mononuclear cells
    Teresa Krakauer
    Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
    Methods Mol Biol 214:151-64. 2003
  24. pmc TNF optimally activatives regulatory T cells by inducing TNF receptor superfamily members TNFR2, 4-1BB and OX40
    Ryoko Hamano
    Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center for Cancer Research, NCI Frederick, Frederick, MD 21702 1201, USA
    Eur J Immunol 41:2010-20. 2011
    ....
  25. pmc Shikonin, a component of chinese herbal medicine, inhibits chemokine receptor function and suppresses human immunodeficiency virus type 1
    Xin Chen
    Basic Research Program, SAIC Frederick, Inc, National Cancer Institute Frederick, Frederick, Maryland 21702 1201, USA
    Antimicrob Agents Chemother 47:2810-6. 2003
    ..Therefore, shikonin, as a naturally occurring, low-molecular-weight pan-chemokine receptor inhibitor, constitutes a basis for the development of novel anti-HIV therapeutic agents...
  26. ncbi request reprint Effects of IL-7 and dexamethasone: induction of CD25, the high affinity IL-2 receptor, on human CD4+ cells
    Il Yup Chung
    Laboratory of Molecular Immunoregulation, Center for Cancer Research, Frederick, MD 21702 1201, USA
    Cell Immunol 232:57-63. 2004
    ..This combination may provide a novel means of priming CD4 T cells to respond to IL-2 and may prove useful in up-regulation of normal immune responses in immune deficient diseases...
  27. pmc Rapamycin inhibits differentiation of Th17 cells and promotes generation of FoxP3+ T regulatory cells
    Heather Kopf
    Laboratory of Molecular Immunoregulation, Center for Cancer Research, NCI Frederick, Frederick, Maryland 21702 1201, USA
    Int Immunopharmacol 7:1819-24. 2007
    ....
  28. pmc In vitro generated Th17 cells support the expansion and phenotypic stability of CD4(+)Foxp3(+) regulatory T cells in vivo
    Qiong Zhou
    Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center for Cancer Research, NCI, Frederick, MD 21702, United States
    Cytokine 65:56-64. 2014
    ..This bidirectional interaction should be taken into account when designing therapy targeting Th17 cells, Tregs, TNF and TNFR2. ..
  29. pmc Autoantigens signal through chemokine receptors: uveitis antigens induce CXCR3- and CXCR5-expressing lymphocytes and immature dendritic cells to migrate
    O M Zack Howard
    National Cancer Institute Center for Cancer Research, Laboratory of Molecular Immunoregulation, Basic Research Program, Science Applications International Corporation, Frederick, MD, USA
    Blood 105:4207-14. 2005
    ..These studies suggest that IRBP and S-Ag can initiate innate and, in sensitive individuals, adaptive immune response by attracting iDCs and T and B cells expressing CXCR3 and CXCR5...
  30. pmc Progranulin promotes tumour necrosis factor-induced proliferation of suppressive mouse CD4⁺ Foxp3⁺ regulatory T cells
    Ya Hu
    Laboratory of Molecular Immunoregulation, Cancer Inflammation Program, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA School of Medicine, Yangtze University, Jingzhou, Hubei, China
    Immunology 142:193-201. 2014
    ..Hence, our data clearly show that PGRN promotes, rather than inhibits, the functional consequence of TNF-TNFR2 interaction on Treg cells. ..
  31. pmc IKKα is required for the homeostasis of regulatory T cells and for the expansion of both regulatory and effector CD4 T cells
    Xin Chen
    Basic Science Program, Leidos Biomedical Research, Incorporated, Laboratory of Molecular Immunoregulation, and State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China
    FASEB J 29:443-54. 2015
    ..These properties of IKKα may be exploited as therapeutic strategies in the treatment of major human diseases...
  32. ncbi request reprint Chemokines and chemokine receptors as novel therapeutic targets in rheumatoid arthritis (RA): inhibitory effects of traditional Chinese medicinal components
    Xin Chen
    Basic Research Program, SAIC Frederick, Inc, MD 21702 1201, USA
    Cell Mol Immunol 1:336-42. 2004
    ..Thus, this approach may lead to the discovery of new inhibitors of chemokines or chemokine receptors that can be used to treat diseases associated with inappropriately overactive chemokine mediated inflammatory reactions...
  33. pmc Novel peptides based on HIV-1 gp120 sequence with homology to chemokines inhibit HIV infection in cell culture
    Oleg Chertov
    Protein Chemistry Laboratory, Advanced Technology Program, SAIC Frederick, Inc, National Cancer Institute at Frederick, Frederick, Maryland, United States of America
    PLoS ONE 6:e14474. 2011
    ..Thus these peptides can be used to dissect gp120 interactions with chemokine receptors and could serve as leads for the design of new inhibitors of HIV-1...
  34. pmc Regulatory T cells and myeloid-derived suppressor cells in the tumor microenvironment undergo Fas-dependent cell death during IL-2/αCD40 therapy
    Jonathan M Weiss
    Cancer and Inflammation Program, National Cancer Institute, Frederick, MD 21702
    J Immunol 192:5821-9. 2014
    ..Our results suggest that immunotherapeutic strategies that include exploiting Treg and MDSC susceptibility to Fas-mediated apoptosis hold promise for treatment of cancer. ..
  35. pmc Effective chemoimmunotherapy with anti-TGFβ antibody and cyclophosphamide in a mouse model of breast cancer
    Xin Chen
    Basic Science Program, Leidos Biomedical Research, Inc, Frederick National Laboratory, Frederick, Maryland, United States of America Laboratory of Molecular Immunoregulation, Center for Cancer Research, National Cancer Institute, Frederick, Maryland, United States of America
    PLoS ONE 9:e85398. 2014
    ..Taken together, our data show that anti-TGFβ antibody and cyclophosphamide represents an effective chemoimmunotherapeutic combination. ..
  36. ncbi request reprint Effects of Shuanghuanglian and Qingkailing, two multi-components of traditional Chinese medicinal preparations, on human leukocyte function
    Xin Chen
    Laboratory of Molecular Immunoregulation, Center for Cancer Research, National Cancer Institute Frederick, Fort Detrick, MD, 21702 1201, USA
    Life Sci 70:2897-913. 2002
    ..Identification of the responsible components in these two herbal preparations may yield compounds suitable for structural modification into potent novel drugs...
  37. ncbi request reprint Cellular pharmacology studies of shikonin derivatives
    Xin Chen
    Laboratory of Molecular Immunoregulation, Division of Basic Sciences, National Cancer Institute Frederick, MD 21702 1201, USA
    Phytother Res 16:199-209. 2002
    ..This general inhibitory effect can account for the broad spectrum of shikonin biological and pharmacological activities...
  38. pmc Contrasting effects of TNF and anti-TNF on the activation of effector T cells and regulatory T cells in autoimmunity
    Xin Chen
    Basic Science Program, SAIC Frederick, Inc, Laboratory of Molecular Immunoregulation, NCI Frederick, Frederick, MD 21702, USA
    FEBS Lett 585:3611-8. 2011
    ..Understanding the differential effects of TNF on Teffs and Tregs is fundamentally required for the design of more effective and safer anti-TNF or anti-TNF receptor(s) therapeutic strategy for autoimmune diseases...
  39. doi request reprint The Alarmin HMGN1 contributes to antitumor immunity and is a potent immunoadjuvant
    Feng Wei
    Laboratory of Molecular Immunoregulation, Cancer and Inflammation Program, Center for Cancer Research, Frederick National Laboratory for Cancer Research FNLCR, Frederick, Maryland Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China
    Cancer Res 74:5989-98. 2014
    ..Overall, our findings show that HMGN1 contributes to antitumor immunity and it may offer an effective adjuvant to heighten responses to cancer vaccines...
  40. ncbi request reprint Regulatory effects of deoxycholic acid, a component of the anti-inflammatory traditional Chinese medicine Niuhuang, on human leukocyte response to chemoattractants
    Xin Chen
    The Laboratory of Molecular Immunoregulation, Center for Cancer Research, National Cancer Institute Frederick, Bldg 560, Rm 31 19, Frederick, MD 21702 1201, USA
    Biochem Pharmacol 63:533-41. 2002
    ..Taken together, our experimental results suggest that blockade of fMLP receptors may contribute to the anti-inflammatory effects of traditional medicine containing DCA...
  41. doi request reprint Imidazo[1,5-a]pyrazines: orally efficacious inhibitors of mTORC1 and mTORC2
    Andrew P Crew
    OSI Oncology, OSI Pharmaceuticals, Inc, 1 Bioscience Park Drive, Farmingdale, NY 11735, USA
    Bioorg Med Chem Lett 21:2092-7. 2011
    ....
  42. pmc Splenic CD19-CD35+B220+ cells function as an inducer of follicular dendritic cell network formation
    Takaya Murakami
    Laboratory for Epithelial Immunobiology, Rikagaku Kenkyusho RIKEN Research Center for Allergy and Immunology, Yokohama, Japan
    Blood 110:1215-24. 2007
    ....
  43. pmc Stimulation of macrophage TNFalpha production by orthopaedic wear particles requires activation of the ERK1/2/Egr-1 and NF-kappaB pathways but is independent of p38 and JNK
    Michelle A Beidelschies
    Department of Physiology and Biophysics, Case Medical Center, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Cell Physiol 217:652-66. 2008
    ....
  44. ncbi request reprint TNFalpha and PTH utilize distinct mechanisms to induce IL-6 and RANKL expression with markedly different kinetics
    Jia C Dai
    Department of Orthopaedics, Case Western Reserve University, Cleveland, OH 44106 5000, USA
    Bone 38:509-20. 2006
    ..In contrast, the less extensive effect of PTH likely reflects that it acts in physiological situations where it is important to minimize the potential adverse effects of high levels of IL-6 on bone and/or surrounding tissues...
  45. pmc The beta-catenin/T-cell factor/lymphocyte enhancer factor signaling pathway is required for normal and stress-induced cardiac hypertrophy
    Xin Chen
    Molecular Cardiology Research Institute, Tufts New England Medical Center and Tufts University School of Medicine, USA
    Mol Cell Biol 26:4462-73. 2006
    ..Thus, our studies, employing complementary models in vivo, implicate beta-catenin/Tcf/Lef signaling as an essential growth-regulatory pathway in terminally differentiated cells...
  46. ncbi request reprint A microarray analysis of the emergence of embryonic definitive hematopoiesis
    Dong Chen
    Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, Wisconsin, USA
    Exp Hematol 35:1344-57. 2007
    ..However, results from ES cell-based studies have been limited by the paucity of information available about early human hematopoietic development...
  47. pmc Gene expression patterns of human colon tops and basal crypts and BMP antagonists as intestinal stem cell niche factors
    Cynthia Kosinski
    Department of Biopharmaceutical Sciences, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 104:15418-23. 2007
    ..Our study also suggests that BMP antagonists are candidate signaling components that make up the intestinal epithelial stem cell niche...
  48. ncbi request reprint Frequency response model and simulation of transmembrane potentials on cellular inner and outer membranes
    Xin Chen
    Key Laboratory of High Voltage Engineering and Electrical New Technology, Ministry of Education, Chongqing University, Chongqing, 400044 China
    Conf Proc IEEE Eng Med Biol Soc 2007:5819-22. 2007
    ..The experimental result agrees well with the simulation. Therefore, the simulation result provides a theoretical guidance to choosing reasonable window parameters for application of nanosecond pulse to tumor treatment...
  49. pmc Curcumin prevents and reverses murine cardiac hypertrophy
    Hong Liang Li
    Division of Cardiology, Heart and Stroke Richard Lewar Centre of Excellence, University Health Network, University of Toronto, Toronto, Ontario, Canada
    J Clin Invest 118:879-93. 2008
    ..Our results indicate that curcumin has the potential to protect against cardiac hypertrophy, inflammation, and fibrosis through suppression of p300-HAT activity and downstream GATA4, NF-kappaB, and TGF-beta-Smad signaling pathways...
  50. doi request reprint Design and evaluation of sifuvirtide, a novel HIV-1 fusion inhibitor
    Yuxian He
    AIDS Research Center, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
    J Biol Chem 283:11126-34. 2008
    ..0 +/- 8.6 h and 26.0 +/- 7.9 h, respectively. In summary, sifuvirtide has potential to become an ideal fusion inhibitor for treatment of HIV/AIDS patients, including those with HIV-1 strains resistant to T20...
  51. doi request reprint Study of transmembrane potentials on cellular inner and outer membrane--frequency response model and its filter characteristic simulation
    Chenguo Yao
    State Key Laboratory of Power Transmission Equipment and System Security and New Technology, College of Electrical Engineering, Chongqing University, Chongqing 400044, China
    IEEE Trans Biomed Eng 55:1792-9. 2008
    ..Therefore, the frequency response model could help to choose reasonable window parameters for the application of a nanosecond pulsed electric field to tumor treatment...
  52. ncbi request reprint Either part of a Drosophila epsin protein, divided after the ENTH domain, functions in endocytosis of delta in the developing eye
    Erin Overstreet
    Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station, 78712, USA
    Curr Biol 13:854-60. 2003
    ..These results challenge the model for epsin function that requires an intact protein...
  53. pmc Parallels between global transcriptional programs of polarizing Caco-2 intestinal epithelial cells in vitro and gene expression programs in normal colon and colon cancer
    Annika M Sääf
    Department of Biochemistry, Stanford University, Stanford, CA 94305, USA
    Mol Biol Cell 18:4245-60. 2007
    ..The full data set is available at http://microarray-pubs.stanford.edu/CACO2...
  54. ncbi request reprint Impaired heart contractility in Apelin gene-deficient mice associated with aging and pressure overload
    Keiji Kuba
    Institute of Molecular Biotechnology, Austrian Academy of Sciences, Vienna, Austria
    Circ Res 101:e32-42. 2007
    ..These genetic data show that the endogenous peptide Apelin is crucial to maintain cardiac contractility in pressure overload and aging...
  55. ncbi request reprint Inverse expression of Jun activation domain binding protein 1 and cell cycle inhibitor p27Kip1: influence on proliferation in hepatocellular carcinoma
    Jan P Berg
    Institute of Pathology, University of Bonn, D 53127 Bonn, Germany
    Hum Pathol 38:1621-7. 2007
    ..We conclude that Jab1 expression may lead to down-regulation of the negative cell cycle regulator p27(Kip1), pointing to a possible mechanism that promotes hepatocarcinogenesis...
  56. ncbi request reprint [The research of relationship between DNA degradation and postmortem interval]
    Xin Chen
    Shanghai Institute of Criminal Science and Technology, Shanghai 200083, China
    Fa Yi Xue Za Zhi 21:115-7. 2005
    ..To investigate the relationship between the DNA degradation in cells and postmortem interval...
  57. pmc Comparison of array-based comparative genomic hybridization with gene expression-based regional expression biases to identify genetic abnormalities in hepatocellular carcinoma
    Kyle A Furge
    Van Andel Research Institute, 333 Bostwick Ave NE, Grand Rapids, MI 49503, USA
    BMC Genomics 6:67. 2005
    ..However as REBs are identified using transcriptional information, detection of REBs may also identify local transcriptional abnormalities produced by both genetic and epigenetic mechanisms...
  58. ncbi request reprint Granulin-epithelin precursor overexpression promotes growth and invasion of hepatocellular carcinoma
    Siu Tim Cheung
    Centre for the Study of Liver Disease and Departments of Surgery and Pathology, The University of Hong Kong, Pokfulam, Hong Kong, China
    Clin Cancer Res 10:7629-36. 2004
    ..The aim of this study was to investigate the clinical significance of GEP expression and its potential as a therapeutic target in HCC...
  59. ncbi request reprint Correlation between gene expression and morphological alterations in baboon carotid after balloon dilatation injury
    Einari Aavik
    Rational Drug Design Program, Biomedicum and Transplantation Laboratory, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland
    FASEB J 19:130-2. 2005
    ..The analysis presents a tentative model for genomic response to balloon catheter injury and a road map to identify time-related genomic alterations in human vascular specimens...
  60. ncbi request reprint Testis-specific TAF homologs collaborate to control a tissue-specific transcription program
    Mark Hiller
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305 5329, USA
    Development 131:5297-308. 2004
    ....
  61. pmc Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumors
    Jamie M Sperger
    Wisconsin National Primate Research Center and Department of Anatomy, School of Medicine, University of Wisconsin, Madison, WI 53715, USA
    Proc Natl Acad Sci U S A 100:13350-5. 2003
    ..These genes are candidates for involvement in the maintenance of a pluripotent, undifferentiated phenotype...
  62. ncbi request reprint Can an in silico drug-target search method be used to probe potential mechanisms of medicinal plant ingredients?
    Xin Chen
    Department of Computational Science, National University of Singapore, Blk S17, Level 7, 3 Science Drive 2, Singapore 117543
    Nat Prod Rep 20:432-44. 2003
    ....
  63. ncbi request reprint [Estimation of postmortal interval by measuring the amount of DNA in cells]
    Xin Chen
    Shanghai Criminal Science and Technology Institute, Shanghai 200083, China
    Fa Yi Xue Za Zhi 19:120-1. 2003
    ..This paper introduce several different measuring ways, law of variation and application situation of DNA in cells. In addition, the result evaluation of measuring methods and application prospect is given...
  64. pmc High incidence of epithelial cancers in mice deficient for DNA polymerase delta proofreading
    Robert E Goldsby
    Eccles Institute of Human Genetics and Department of Pediatrics Division of Pediatric HematologyOncology, University of Utah School of Medicine, Salt Lake City 84112, USA
    Proc Natl Acad Sci U S A 99:15560-5. 2002
    ..Mice deficient in poldelta proofreading provide a tractable model to study mechanisms of epithelial tumorigenesis initiated by a mutator phenotype...
  65. ncbi request reprint BMP4 initiates human embryonic stem cell differentiation to trophoblast
    Ren He Xu
    WiCell Research Institute, Madison, WI 53715, USA
    Nat Biotechnol 20:1261-4. 2002
    ..Human ES cells thus provide a tool for studying the differentiation and function of early human trophoblast and could provide a new understanding of some of the earliest differentiation events of human postimplantation development...
  66. ncbi request reprint [IRE_FINDER-computational search of iron response element in human and mouse UTRs]
    Xin Chen
    College of Life Science, Peking University, Beijing 100871, China
    Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 34:743-7. 2002
    ..Here we use an improved computation method performing a comprehensive search of IRE in human and mouse genes. We try to catalog potential human and mouse IRE containing genes, at the same time identify potential U1A5 IREs...
  67. ncbi request reprint Termination of immediate-early gene expression after stimulation by parathyroid hormone or isoproterenol
    Xin Chen
    Department of Orthopaedics, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, Ohio 44106 5000, USA
    Am J Physiol Cell Physiol 283:C1432-40. 2002
    ..This conclusion may therefore reflect a general mechanism for termination of immediate-early gene expression after induction by cAMP/PKA...
  68. ncbi request reprint [The effects of interleukin-12 inhalation on airway inflammation and T helper cell subsets in mouse asthma models]
    Xin Chen
    Respiratory Department of China Japan Friendship Hospital, Beijing 100029, China
    Zhonghua Nei Ke Za Zhi 41:313-6. 2002
    ..To observe the effects of aerosol interleukin-12 (IL-12) on the antigen-induced airway inflammation and T helper cell subsets in mouse asthma models...
  69. ncbi request reprint [Rosiglitazone inhibits atherosclerosis in apolipoprotein E-knockout mice]
    Quan Ming Zhao
    Department of Cardiology, Beijing Anzhen Hospital, Capital University of Medical Science, Beijing 100029, China
    Zhonghua Xin Xue Guan Bing Za Zhi 33:399-404. 2005
    ..To study the effect of rosiglitazone on atherosclerosis and potential mechanism in ApoE-knockout mice...
  70. ncbi request reprint Array-based comparative genomic hybridization reveals recurrent chromosomal aberrations and Jab1 as a potential target for 8q gain in hepatocellular carcinoma
    Mohini A Patil
    Department of Biopharmaceutical Sciences, University of California, San Francisco, CA 94143, USA
    Carcinogenesis 26:2050-7. 2005
    ....
  71. ncbi request reprint Radiogenomic analysis to identify imaging phenotypes associated with drug response gene expression programs in hepatocellular carcinoma
    Michael D Kuo
    Department of Radiology, University of California, San Diego Medical Center, San Diego, CA 92103, USA
    J Vasc Interv Radiol 18:821-31. 2007
    ....
  72. pmc Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage
    Matthew T Pankratz
    Waisman Center, Rm T613, University of Wisconsin, 1500 Highland Ave, Madison, Wisconsin 53705, USA
    Stem Cells 25:1511-20. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  73. ncbi request reprint The stochastic nature of gene expression revealed at the single-molecule level
    Xin Chen
    Department of Chemistry, Texas A and M University, 3255 TAMU, College Station, Texas 77843, USA
    ACS Chem Biol 1:129-31. 2006
    ..The work was done by two separate methods: fluorescent protein labeling and enzymatic amplification. Statistical analysis of the data reveals the inherent stochastic nature of gene expression...
  74. ncbi request reprint Microarray analysis of stem cells and differentiation
    Howard Y Chang
    Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA
    Methods Enzymol 420:225-54. 2006
    ..We also detail examples that apply microarray technology to address several of the main questions in stem cell biology...
  75. ncbi request reprint [The effects of nerve growth factor-induced apoptosis on hepatic stellate cells of fibrotic rat liver]
    Xin Chen
    Department of Gastroenterology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China
    Zhonghua Yi Xue Za Zhi 86:1985-8. 2006
    ..To evaluate the effects of nerve growth factor (NGF) on the proliferation and apoptosis of hepatic stellate cells (HSCs) of fibrotic rat...
  76. ncbi request reprint Biocompatibility of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) modified by silk fibroin
    Na Mei
    The Key Laboratory of Molecular Engineering of Polymers, Ministry of Education, Macromolecular Science Department, Fudan University, Shanghai, 200433, China
    J Mater Sci Mater Med 17:749-58. 2006
  77. ncbi request reprint Structure-based drug design of a novel family of PPARgamma partial agonists: virtual screening, X-ray crystallography, and in vitro/in vivo biological activities
    I Lin Lu
    Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Taipei, Taiwan, ROC
    J Med Chem 49:2703-12. 2006
    ..e., PPARalpha and PPARdelta, and steric hindrance upon the ligand binding...
  78. ncbi request reprint Chromatin-binding in vivo of the erythroid kruppel-like factor, EKLF, in the murine globin loci
    Yu Chiau Shyu
    Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 115
    Cell Res 16:347-55. 2006
    ....
  79. ncbi request reprint Tricyclic azepine derivatives: pyrimido[4,5-b]-1,4-benzoxazepines as a novel class of epidermal growth factor receptor kinase inhibitors
    Leon Smith
    Department of Chemistry, ImClone Systems, 180 Varick Street, New York, NY 10014, USA
    Bioorg Med Chem Lett 16:1643-6. 2006
    ..Such compounds demonstrate anti-EGFR activity within a class that is different from any known EGFR inhibitor scaffolds. They also provide a basis for the design of kinase inhibitors with the desired selectivity profile...
  80. ncbi request reprint Regional activation of chromosomal arm 7q with and without gene amplification in taxane-selected human ovarian cancer cell lines
    Yan C Wang
    Oncology Division, Department of Medicine, Stanford University School of Medicine, Stanford, California 94305, USA
    Genes Chromosomes Cancer 45:365-74. 2006
    ....
  81. ncbi request reprint Novel indole-based peroxisome proliferator-activated receptor agonists: design, SAR, structural biology, and biological activities
    Neeraj Mahindroo
    Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, Republic of China
    J Med Chem 48:8194-208. 2005
    ..Structural biology studies of 14 showed that the indole ring contributes strong hydrophobic interactions with PPARgamma and could be an important moiety for the binding to the protein...
  82. ncbi request reprint Tissue-specific TAFs counteract Polycomb to turn on terminal differentiation
    Xin Chen
    Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, CA 94305 5329, USA
    Science 310:869-72. 2005
    ..Testis TAFs also promoted relocalization of Polycomb Repression Complex 1 components to the nucleolus in spermatocytes, implicating subnuclear architecture in the regulation of terminal differentiation...
  83. ncbi request reprint Synthesis and structure-activity relationships of pyrazine-pyridine biheteroaryls as novel, potent, and selective vascular endothelial growth factor receptor-2 inhibitors
    Gee Hong Kuo
    Drug Discovery Division, Johnson and Johnson Pharmaceutical Research and Development, L L C, 1000 Route 202, P O Box 300, Raritan, New Jersey 08869, USA
    J Med Chem 48:4892-909. 2005
    ..Molecular modeling (QSAR analysis) was conducted in an attempt to rationalize the observed structure-activity relationship...
  84. ncbi request reprint A P element transformation vector for high levels of gene expression in germ-line cells of the ovary and undifferentiated cells in the developing eye of Drosophila
    Xin Chen
    Section of Molecular Cell and Developmental Biology, The Institute for Cellular and Molecular Biology, Austin, Texas 78712, USA
    Plasmid 47:61-5. 2002
    ..In addition, in the eye, pFAF is unique in that it activates transcription specifically in undifferentiated cells...