Affiliation: Nash and Associates
- Hexa-arginine enhanced uptake and residualization of selective high affinity ligands by Raji lymphoma cellsRod Balhorn
University of California, Department of Applied Science, Hertz Hall, Livermore, CA 94551, USA
Mol Cancer 8:25. 2009....
- Selective high-affinity ligand antibody mimics for cancer diagnosis and therapy: initial application to lymphoma/leukemiaRod Balhorn
Chemistry, Materials and Life Sciences and Computations, Lawrence Livermore National Laboratory, Livermore, California, 94550, USA
Clin Cancer Res 13:5621s-5628s. 2007....
- Recombinant expression of the beta-subunit of HLA-DR10 for the selection of novel lymphoma targeting moleculesHuguette Albrecht
Cancer Center, University of California, Davis Medical Center, Sacramento, CA 95816, USA
Cancer Biother Radiopharm 22:531-42. 2007..Therefore, this recombinant protein can be used as a surrogate for native heterodimeric HLA DR10 for the in vitro selection of SHALs and related targeting molecules...
- Nanomolecular HLA-DR10 antibody mimics: A potent system for molecular targeted therapy and imagingGerald L DeNardo
University of California, Davis Medical Center, Sacramento, USA
Cancer Biother Radiopharm 23:783-96. 2008..Further, these SHALs can be used to transport and residualize cytotoxic agents near critical sites inside these malignant cells...
- Identification of a thermo-regulated glutamine-binding protein from Yersinia pestisMonique Cosman
Lawrence Livermore National Laboratory, Livermore, CA 94551, USA
Protein Pept Lett 15:887-94. 2008..The identification and characterization of an unknown protein function using the strategy presented here has applicability to a variety of research areas, including functional genomics and proteomics efforts...
- Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cellsThomas Huser
NSF Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, CA 95817, USA
J Biophotonics 2:322-32. 2009..These findings indicate that single cell Raman spectroscopy should be a valuable tool in assessing the quality of sperm cells for in-vitro fertilization...
- Dynamics of protamine 1 binding to single DNA moleculesLaurence Brewer
Electronic Engineering Technologies Division, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA
J Biol Chem 278:42403-8. 2003..The use of Arg, rather than Lys residues, and the inclusion of Tyr or Phe residues in the hinge regions between anchoring domains provide additional stability to the complex...
- Electrospray mass spectrometry of NeuAc oligomers associated with the C fragment of the tetanus toxinMaria C Prieto Conway
Chemistry and Materials Science Directorate, Lawrence Livermore National Laboratory, Livermore, California 94550, USA
J Am Soc Mass Spectrom 17:967-76. 2006....
- Systemic radiotherapy can cure lymphoma: a paradigm for other malignancies?Gerald L DeNardo
Department of Internal Medicine, University of California, Davis, Sacramento, CA 95816, USA
Cancer Biother Radiopharm 23:383-97. 2008..Smaller radionuclide carriers, such as those used for pretargeted strategies, provide dose intensification. The potential of pretargeted RIT to improve patient outcomes is striking...
- Synthesis and radiolabeling of selective high-affinity ligands designed to target non-Hodgkin's lymphoma and leukemiaSaphon Hok
Chemistry, Materials and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, California 94551, USA
Bioconjug Chem 18:912-21. 2007..Radiolabeling reactions of DOTA-SHALs, with exactly one DOTA entity per targeting SHAL molecule, yielded products with greater than 90% radiochemical purity and specific activities ranging from 97 to 150 muCi/mug...
- Mass spectrometry and non-covalent protein-ligand complexes: confirmation of binding sites and changes in tertiary structureSharon J Shields
Chemistry and Materials Science, Lawrence Livermore National Laboratory, Livermore, California 94551, USA
J Am Soc Mass Spectrom 14:460-70. 2003..These five peptides flank regions of decreased ion abundance, suggesting that doxorubicin not only binds to the surface, but also induces a conformational change in TetC...
- Computational approaches for identification of conserved/unique binding pockets in the A chain of ricinCarol L Ecale Zhou
Lawrence Livermore National Laboratory 7000 E Avenue, Livermore, CA 94550, USA
Bioinformatics 21:3089-96. 2005..To reduce the time and cost of laboratory screening efforts for diagnostic reagents, we developed new methods for evaluating and selecting protein surface regions for ligand targeting...
- Application of NMR methods to identify detection reagents for use in development of robust nanosensorsMonique Cosman
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA, USA
Methods Mol Biol 300:141-63. 2005..Using this assay, one can identify the optimal pairs of ligands to be linked together for creating detection reagents, as well as estimate the relative binding constants for ligands competing for the same site...
- Mechanism of DNA compaction by yeast mitochondrial protein Abf2pRaymond W Friddle
Biosecurity and Nanoscience Laboratory, Chemistry and Materials Science Directorate, Lawrence Livermore National Laboratory, Livermore, California, USA
Biophys J 86:1632-9. 2004..These studies indicate that Abf2p compacts DNA through a simple mechanism that involves bending of the DNA backbone. We discuss the implications of such a mechanism for mitochondrial DNA maintenance and organization...
- The protamine family of sperm nuclear proteinsRod Balhorn
Biosciences and Biotechnology Division, Chemistry, Materials and Life Sciences, Lawrence Livermore National Laboratory, East Avenue, Livermore, CA 94550, USA
Genome Biol 8:227. 2007..Both P1 and P2 have been shown to be required for normal sperm function in primates and many rodents...
- Crystal structure of Clostridium botulinum neurotoxin protease in a product-bound state: Evidence for noncanonical zinc protease activityBrent Segelke
University of California, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94551, USA
Proc Natl Acad Sci U S A 101:6888-93. 2004..The proposed BoNT/A substrate-binding mode and catalytic mechanism are markedly different from those previously proposed for the BoNT serotype B...
- Identification of novel small molecules that bind to two different sites on the surface of tetanus toxin C fragmentMonique Cosman
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, California 94551 0808, USA
Chem Res Toxicol 15:1218-28. 2002....
- Monitoring dynamic protein expression in living E. coli. Bacterial cells by laser tweezers Raman spectroscopyJames W Chan
Applied Physics and Biophysics Division, Physics and Advanced Technologies Directorate, Lawrence Livermore National Laboratory, Livermore, California 94551, USA
Cytometry A 71:468-74. 2007..Here, we demonstrate its use to monitor changes in a population of E. coli cells that occur during overexpression of a protein, the extracellular domain of myelin oligodendrocyte glycoprotein [MOG(1-120)]...
- Self-assembly of heptameric nanoparticles derived from tag-functionalized phi29 connectorsDominik J Green
Department of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
ACS Nano 4:7651-9. 2010..By modifying the surface-exposed C-terminal ends with application-appropriate moieties, the consistent structure and compact nature of these nanoparticles may prove beneficial in nanotechnological and nanomedical approaches...