K R Gee

Summary

Affiliation: Molecular Probes
Country: USA

Publications

  1. ncbi request reprint Fluorescent Bocillins: synthesis and application in the detection of penicillin-binding proteins
    K R Gee
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Electrophoresis 22:960-5. 2001
  2. ncbi request reprint Caged Q-rhodamine dextran: a new photoactivated fluorescent tracer
    K R Gee
    Molecular Probes, Inc, 4849 Pitchford Avenue, Eugene, OR 97402, USA
    Bioorg Med Chem Lett 11:2181-3. 2001
  3. ncbi request reprint Measuring zinc in living cells. A new generation of sensitive and selective fluorescent probes
    K R Gee
    Molecular Probes, Inc, 4849 Pitchford Avenue, Eugene, OR 97478, USA
    Cell Calcium 31:245-51. 2002
  4. ncbi request reprint New Ca2+ fluoroionophores based on the BODIPY fluorophore
    Kyle R Gee
    Molecular Probes, Inc, 29851 Willow Creek Rd, Eugene, OR 97402, USA
    Comb Chem High Throughput Screen 6:363-6. 2003
  5. ncbi request reprint Quantitative analysis of protein phosphorylation status and protein kinase activity on microarrays using a novel fluorescent phosphorylation sensor dye
    Karen Martin
    Proteomics Section, Molecular Probes Inc, 29851 Willow Creek Road, Eugene, OR 97402, USA
    Proteomics 3:1244-55. 2003
  6. ncbi request reprint Redox-dependent trafficking of 2,3,4,5, 6-pentafluorodihydrotetramethylrosamine, a novel fluorogenic indicator of cellular oxidative activity
    C S Chen
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Free Radic Biol Med 28:1266-78. 2000
  7. ncbi request reprint New ratiometric fluorescent calcium indicators with moderately attenuated binding affinities
    K R Gee
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Bioorg Med Chem Lett 10:1515-8. 2000
  8. ncbi request reprint Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes
    K R Gee
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Cell Calcium 27:97-106. 2000
  9. ncbi request reprint Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes
    W C Sun
    Molecular Probes, Inc, Eugene, Oregon 97402 9165, USA
    Bioorg Med Chem Lett 8:3107-10. 1998
  10. ncbi request reprint Control of NO concentration in solutions of nitrosothiol compounds by light
    V R Zhelyaskov
    World Precision Instruments, Inc, International Trade Center, Sarasota, FL 34240 9258, USA
    Photochem Photobiol 67:282-8. 1998

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Fluorescent Bocillins: synthesis and application in the detection of penicillin-binding proteins
    K R Gee
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Electrophoresis 22:960-5. 2001
    ..These reagents allow for facile detection of 2-4 ng of purified PBP with the aid of a fluorescent scanner...
  2. ncbi request reprint Caged Q-rhodamine dextran: a new photoactivated fluorescent tracer
    K R Gee
    Molecular Probes, Inc, 4849 Pitchford Avenue, Eugene, OR 97402, USA
    Bioorg Med Chem Lett 11:2181-3. 2001
    ..The resulting fluorescent cell was imaged by fluorescence microscopy through a single round of cell division...
  3. ncbi request reprint Measuring zinc in living cells. A new generation of sensitive and selective fluorescent probes
    K R Gee
    Molecular Probes, Inc, 4849 Pitchford Avenue, Eugene, OR 97478, USA
    Cell Calcium 31:245-51. 2002
    ..Experiments in neuronal and non-neuronal cell cultures show the new indicators to retain their sensitivity to and selectivity for zinc after conversion to cell-permeable forms...
  4. ncbi request reprint New Ca2+ fluoroionophores based on the BODIPY fluorophore
    Kyle R Gee
    Molecular Probes, Inc, 29851 Willow Creek Rd, Eugene, OR 97402, USA
    Comb Chem High Throughput Screen 6:363-6. 2003
    ..Cell permeable versions respond well with increasing fluorescence intensities in live cells after calcium influx...
  5. ncbi request reprint Quantitative analysis of protein phosphorylation status and protein kinase activity on microarrays using a novel fluorescent phosphorylation sensor dye
    Karen Martin
    Proteomics Section, Molecular Probes Inc, 29851 Willow Creek Road, Eugene, OR 97402, USA
    Proteomics 3:1244-55. 2003
    ....
  6. ncbi request reprint Redox-dependent trafficking of 2,3,4,5, 6-pentafluorodihydrotetramethylrosamine, a novel fluorogenic indicator of cellular oxidative activity
    C S Chen
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Free Radic Biol Med 28:1266-78. 2000
    ..Based on its subcellular distribution and its oxidation by a broad range of oxidizing species, PF-H(2)TMRos is demonstrated to be a novel indicator for cellular oxidative stresses...
  7. ncbi request reprint New ratiometric fluorescent calcium indicators with moderately attenuated binding affinities
    K R Gee
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Bioorg Med Chem Lett 10:1515-8. 2000
    ..Two new excitation ratioable fluorescent calcium indicators, benzothiaza-1 and 2, were also synthesized. Kd values of 400 nM to 5.3 microM [Ca2+] were observed in these families of new probes...
  8. ncbi request reprint Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes
    K R Gee
    Molecular Probes, Inc, Eugene, OR 97402, USA
    Cell Calcium 27:97-106. 2000
    ..Because of its higher fluorescence emission intensity, fluo-4 can be used at lower intracellular concentrations, making its use a less invasive practice...
  9. ncbi request reprint Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes
    W C Sun
    Molecular Probes, Inc, Eugene, Oregon 97402 9165, USA
    Bioorg Med Chem Lett 8:3107-10. 1998
    ..63 to 0.89) equal to or higher than that of the parent compound (0.63), which, in combination with their lower pKaS and higher photostability, make them superior fluorescent dyes for use as reporter molecules in biological systems...
  10. ncbi request reprint Control of NO concentration in solutions of nitrosothiol compounds by light
    V R Zhelyaskov
    World Precision Instruments, Inc, International Trade Center, Sarasota, FL 34240 9258, USA
    Photochem Photobiol 67:282-8. 1998
    ..Generation of superoxide as a product of a photolytic decomposition reaction of S-nitrosothiols and further oxidation of NO is the most likely mechanism for light suppression of NO concentration...
  11. pmc Spontaneous nucleotide exchange in low molecular weight GTPases by fluorescently labeled gamma-phosphate-linked GTP analogs
    Jonas Korlach
    Field of Biochemistry, Molecular and Cell Biology, School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
    Proc Natl Acad Sci U S A 101:2800-5. 2004
    ..Together, these results validate the use of these analogs as valuable tools for studying GTPase functions and for developing potent synthetic nucleotide exchange factors for this important class of signaling molecules...
  12. ncbi request reprint Fluorescent sodium ion indicators based on the 1,7-diaza-15-crown-5 system
    Vladimir V Martin
    Molecular Probes, Inc, 29851 Willow Creek Road, Eugene, OR 97402, USA
    Bioorg Med Chem Lett 14:5313-6. 2004
    ..The chelator nitrogen atom substituents affect the selectivity and affinity of cation binding, while the nature of the fluorophore determines the type of fluorescent response to metal ion chelation...
  13. ncbi request reprint Simultaneous monitoring of Zn2+ secretion and intracellular Ca2+ from islets and islet cells by fluorescence microscopy
    Wei Jun Qian
    Pacific Northwest National Laboratory, Richland, WA, USA
    Biotechniques 37:922-4, 926, 928-30 passim. 2004
    ..This allowed Ca2+ and secretion to be observed with approximately 2 s temporal resolution. This method should be useful for studying Ca2+ secretion coupling and any application, requiring rapid assays of secretion...
  14. ncbi request reprint Fluorescent metal ion indicators based on benzoannelated crown systems: a green fluorescent indicator for intracellular sodium ions
    Vladimir V Martin
    Molecular Probes Invitrogen Detection Technologies, 29851 Willow Creek Road, Eugene, OR 97402, USA
    Bioorg Med Chem Lett 15:1851-5. 2005
    ..A compound having a 15-crown-5 chelator directly attached to a 2,7-difluoroxanthenone fluorophore loads into live cells and responds to sodium ion concentration changes with large fluorescence increases in the visible wavelength range...
  15. ncbi request reprint A new mitochondrial fluorescent zinc sensor
    Stefano L Sensi
    Department of Neurology, University of California, Irvine, CA 92697 4292, USA
    Cell Calcium 34:281-4. 2003
    ..Experiments in neuronal cell cultures show that RhodZin-3 effectively localizes into mitochondria and detects changes of intramitochondrial free Zn2+ ([Zn2+]m)...
  16. ncbi request reprint Global quantitative phosphoprotein analysis using Multiplexed Proteomics technology
    Thomas H Steinberg
    Proteomics Section, Molecular Probes Inc, 29851 Willow Creek Road, Eugene, OR 97402, USA
    Proteomics 3:1128-44. 2003
    ..The linear responses of the fluorescence dyes utilized, allow rigorous quantitation of changes over an unprecedented 500-1000-fold concentration range...
  17. ncbi request reprint Strategies and solid-phase formats for the analysis of protein and peptide phosphorylation employing a novel fluorescent phosphorylation sensor dye
    Karen Martin
    Molecular Probes, Inc, Eugene, Oregon 97402, USA
    Comb Chem High Throughput Screen 6:331-9. 2003
    ..The fluorescence-based detection technology is easy to conduct, cost effective and allows rapid large-scale screening of protein and peptide phosphorylation in a variety of solid-phase assay formats...
  18. ncbi request reprint Fluorescence approaches to study G protein mechanisms
    Dyke P McEwen
    Department of Pharmacology, University of Michigan, Ann Arbor, Michigan 48109, USA
    Methods Enzymol 344:403-20. 2002
  19. ncbi request reprint Detection and imaging of zinc secretion from pancreatic beta-cells using a new fluorescent zinc indicator
    Kyle R Gee
    Molecular Probes, Inc, 4849 Pitchford Avenue, Eugene, Oregon 97402, USA
    J Am Chem Soc 124:776-8. 2002
    ..Also, within secreting cells some regions of the cell membrane gave rise to secretion while others did not, suggesting active zones of secretion on the cell surface...
  20. ncbi request reprint An ultrasensitive, continuous assay for xylanase using the fluorogenic substrate 6,8-difluoro-4-methylumbelliferyl beta-D-xylobioside
    Yue Ge
    Invitrogen Corp, 29851 Willow Creek Road, Eugene, OR 97402, USA
    Anal Biochem 362:63-8. 2007
    ....
  21. ncbi request reprint Novel fluo-4 analogs for fluorescent calcium measurements
    Vladimir V Martin
    Molecular Probes Invitrogen Detection Technologies, 29851 Willow Creek Rd, Eugene, OR 97492, USA
    Cell Calcium 36:509-14. 2004
    ..The biotinylated version of fluo-4 formed a persistent streptavidin complex which still responded to increasing calcium concentrations with a large fluorescence increase...
  22. pmc Modulation of mitochondrial function by endogenous Zn2+ pools
    Stefano L Sensi
    Department of Neurology, University of California, Irvine 92697 4292, USA
    Proc Natl Acad Sci U S A 100:6157-62. 2003
    ..Finally, strong mobilization of protein-bound Zn(2+) appeared to induce partial loss of Deltapsi(m), suggesting that movement of Zn(2+) between cytosolic and mitochondrial pools might be of functional significance in intact neurons...
  23. ncbi request reprint Presynaptic calcium measurements at physiological temperatures using a new class of dextran-conjugated indicators
    Michael Beierlein
    Department of Neurobiology, Harvard Medical School, 220 Longwood Ave, Boston, MA 02115, USA
    J Neurophysiol 92:591-9. 2004
    ..Thus we find that dextran-conjugated indicators are well suited to long-term recordings of Ca(pre) at physiological temperatures and that the development of new red indicators greatly extends their utility...
  24. ncbi request reprint Kinetic and mechanistic studies of a cell cycle protein phosphatase Cdc14
    Wei Qing Wang
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 279:30459-68. 2004
    ..We also identified several residues including Asp50, Asp129, Glu168, Glu171, and Asp177 in the Cdc14 active site cleft that are required for efficient dephosphorylation of hCdh1...
  25. ncbi request reprint Imaging of Zn2+ release from pancreatic beta-cells at the level of single exocytotic events
    Wei Jun Qian
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    Anal Chem 75:3468-75. 2003
    ....
  26. doi request reprint Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodies
    Suzanne B Buck
    Invitrogen Corporation, Eugene, OR 97402, USA
    Biotechniques 44:927-9. 2008
    ..Elimination of this requirement results in the preservation of helical DNA structure and other cell surface epitopes, decreased assay time, and increased reproducibility...