Affiliation: Merck Research Laboratories
- Toward consistent and productive complex media for industrial fermentations: studies on yeast extract for a recombinant yeast fermentation processJinyou Zhang
Bioprocess R and D, Merck Research Laboratories, PO Box 2000, Rahway, New Jersey 07065, USA
Biotechnol Bioeng 82:640-52. 2003....
- Development of Animal-free, Protein-Free and Chemically-Defined Media for NS0 Cell CultureJinyou Zhang
Bioprocess R and D, Merck Research Laboratories, PO Box 2000, 07065, Rahway, NJ, USA
Cytotechnology 48:59-74. 2005..The medium also served well for cryopreservation of NS0 cells in the absence of serum...
- Cholesterol delivery to NS0 cells: challenges and solutions in disposable linear low-density polyethylene-based bioreactorsJessica Okonkowski
Merck Research Laboratories, Bioprocess R and D, PO Box 2000, RY80Y 105, Rahway, NJ 07065, USA
J Biosci Bioeng 103:50-9. 2007..Controlling and mitigating these negative interactions enabled the routine utilization of disposable bioreactors for the cultivation of cholesterol-dependent NS0 cell lines in conjunction with an animal component-free cultivation medium...
- A novel function for selenium in biological system: selenite as a highly effective iron carrier for Chinese hamster ovary cell growth and monoclonal antibody productionJinyou Zhang
Bioprocess R and D, Merck and Co, Inc, Rahway, New Jersey 07065, USA
Biotechnol Bioeng 95:1188-97. 2006..The preparation of the commercially unavailable iron-selenite compound from respective ions, and its effectiveness in cell-culture performance, were dependent on reaction time, substrates, and other conditions...
- Novel micro-bioreactor high throughput technology for cell culture process development: Reproducibility and scalability assessment of fed-batch CHO culturesAshraf Amanullah
Vaccine and Biologics Process Development, Merck Research Laboratories, Merck and Co Inc, West Point, Pennsylvania 19486, USA
Biotechnol Bioeng 106:57-67. 2010..Cell growth, process parameters, metabolic and protein titer profiles were also compared to those from shake flask, bench-top, and pilot scale bioreactor cultivations and found to be within +/-20% of the historical averages...
- Use of glucose feeding to produce concentrated yeast cellsBeth H Junker
Bioprocess R and D, Merck Research Laboratories, Merck and Co, Inc, Rahway, NJ, 07065, USA
Appl Biochem Biotechnol 97:63-78. 2002..These results suggest that defined medium developed at the laboratory scale may need to be further optimized at the pilot scale for equivalent performance...