David G McLaren

Summary

Affiliation: Merck Research Laboratories
Country: USA

Publications

  1. pmc Evaluation of CETP activity in vivo under non-steady-state conditions: influence of anacetrapib on HDL-TG flux
    David G McLaren
    Merck Research Laboratories, Merck and Co Inc, Kenilworth, NJ 07033
    J Lipid Res 57:398-409. 2016
  2. doi request reprint Use of [13C18] oleic acid and mass isotopomer distribution analysis to study synthesis of plasma triglycerides in vivo: analytical and experimental considerations
    David G McLaren
    Merck Research Laboratories, Merck and Co, Inc, Kenilworth, New Jersey 07033, USA
    Anal Chem 85:6287-94. 2013
  3. pmc The use of stable-isotopically labeled oleic acid to interrogate lipid assembly in vivo: assessing pharmacological effects in preclinical species
    David G McLaren
    Merck Research Laboratories, Merck and Co, Inc Rahway, NJ, USA
    J Lipid Res 52:1150-61. 2011
  4. pmc Tracking fatty acid kinetics in distinct lipoprotein fractions in vivo: a novel high-throughput approach for studying dyslipidemia in rodent models
    David G McLaren
    Merck Research Laboratories, Rahway, NJ 07065, USA
    J Lipid Res 54:276-81. 2013
  5. doi request reprint Small molecule activation of lecithin cholesterol acyltransferase modulates lipoprotein metabolism in mice and hamsters
    Zhu Chen
    Cardiovascular Diseases, Merck Research Laboratories, Rahway, NJ 07065, USA
    Metabolism 61:470-81. 2012
  6. doi request reprint New methodologies for studying lipid synthesis and turnover: looking backwards to enable moving forwards
    Stephen F Previs
    Molecular Biomarkers, Merck, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA Electronic address
    Biochim Biophys Acta 1842:402-13. 2014
  7. doi request reprint Demonstration of diet-induced decoupling of fatty acid and cholesterol synthesis by combining gene expression array and 2H2O quantification
    Kristian K Jensen
    Department of Atherosclerosis, Merck Research Labs, Merck and Co, Inc, 126 E Lincoln Ave, Rahway, NJ 07065, USA
    Am J Physiol Endocrinol Metab 302:E209-17. 2012
  8. pmc Anacetrapib promotes reverse cholesterol transport and bulk cholesterol excretion in Syrian golden hamsters
    Jose Castro-Perez
    Department of Cardiovascular Diseases, Atherosclerosis, Merck Research Laboratories, Rahway, NJ, USA
    J Lipid Res 52:1965-73. 2011
  9. doi request reprint Effect of Error Propagation in Stable Isotope Tracer Studies: An Approach for Estimating Impact on Apparent Biochemical Flux
    Stephen F Previs
    Merck Research Laboratories, Kenilworth, New Jersey, USA Electronic address
    Methods Enzymol 561:331-58. 2015
  10. doi request reprint Quantitative profiling of oxylipins in plasma and atherosclerotic plaques of hypercholesterolemic rabbits
    Lazar A Bojic
    Merck and Co, Inc, Merck Research Laboratories, Kenilworth, NJ, 07033, USA
    Anal Bioanal Chem 408:97-105. 2016

Collaborators

Detail Information

Publications23

  1. pmc Evaluation of CETP activity in vivo under non-steady-state conditions: influence of anacetrapib on HDL-TG flux
    David G McLaren
    Merck Research Laboratories, Merck and Co Inc, Kenilworth, NJ 07033
    J Lipid Res 57:398-409. 2016
    ....
  2. doi request reprint Use of [13C18] oleic acid and mass isotopomer distribution analysis to study synthesis of plasma triglycerides in vivo: analytical and experimental considerations
    David G McLaren
    Merck Research Laboratories, Merck and Co, Inc, Kenilworth, New Jersey 07033, USA
    Anal Chem 85:6287-94. 2013
    ....
  3. pmc The use of stable-isotopically labeled oleic acid to interrogate lipid assembly in vivo: assessing pharmacological effects in preclinical species
    David G McLaren
    Merck Research Laboratories, Merck and Co, Inc Rahway, NJ, USA
    J Lipid Res 52:1150-61. 2011
    ....
  4. pmc Tracking fatty acid kinetics in distinct lipoprotein fractions in vivo: a novel high-throughput approach for studying dyslipidemia in rodent models
    David G McLaren
    Merck Research Laboratories, Rahway, NJ 07065, USA
    J Lipid Res 54:276-81. 2013
    ..We expect that it is possible to translate our approach for application in other systems, including studies in humans...
  5. doi request reprint Small molecule activation of lecithin cholesterol acyltransferase modulates lipoprotein metabolism in mice and hamsters
    Zhu Chen
    Cardiovascular Diseases, Merck Research Laboratories, Rahway, NJ 07065, USA
    Metabolism 61:470-81. 2012
    ....
  6. doi request reprint New methodologies for studying lipid synthesis and turnover: looking backwards to enable moving forwards
    Stephen F Previs
    Molecular Biomarkers, Merck, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA Electronic address
    Biochim Biophys Acta 1842:402-13. 2014
    ..This article is part of a Special Issue entitled: Modulation of Adipose Tissue in Health and Disease. ..
  7. doi request reprint Demonstration of diet-induced decoupling of fatty acid and cholesterol synthesis by combining gene expression array and 2H2O quantification
    Kristian K Jensen
    Department of Atherosclerosis, Merck Research Labs, Merck and Co, Inc, 126 E Lincoln Ave, Rahway, NJ 07065, USA
    Am J Physiol Endocrinol Metab 302:E209-17. 2012
    ..In conclusion, by applying gene expression analysis and tracer methodology, we show that fatty acid and cholesterol synthesis are differentially regulated when the carbohydrate intake in mice is altered...
  8. pmc Anacetrapib promotes reverse cholesterol transport and bulk cholesterol excretion in Syrian golden hamsters
    Jose Castro-Perez
    Department of Cardiovascular Diseases, Atherosclerosis, Merck Research Laboratories, Rahway, NJ, USA
    J Lipid Res 52:1965-73. 2011
    ....
  9. doi request reprint Effect of Error Propagation in Stable Isotope Tracer Studies: An Approach for Estimating Impact on Apparent Biochemical Flux
    Stephen F Previs
    Merck Research Laboratories, Kenilworth, New Jersey, USA Electronic address
    Methods Enzymol 561:331-58. 2015
    ..Likewise, one can facilitate biological studies by estimating the reduction in the noise of an outcome that is expected for a given increase in the number of replicate injections. ..
  10. doi request reprint Quantitative profiling of oxylipins in plasma and atherosclerotic plaques of hypercholesterolemic rabbits
    Lazar A Bojic
    Merck and Co, Inc, Merck Research Laboratories, Kenilworth, NJ, 07033, USA
    Anal Bioanal Chem 408:97-105. 2016
    ..The studies reported here make the first step towards a comprehensive characterization of oxylipins as potentially translatable biomarkers of atherosclerosis...
  11. doi request reprint Headspace analyses of ²H labeling of acetone: enabling studies of fatty acid oxidation in vivo
    Ablatt Mahsut
    Exploratory Biomarkers Atherosclerosis, Merck Research Laboratories, Rahway, NJ 07065, USA
    Anal Biochem 408:351-3. 2011
    ..This method enables routine measurements of fatty acid oxidation in rodents; that is, one administers a ²H-labeled fatty acid(s) and then quantifies the production of ²H-labeled water...
  12. pmc Localization of fatty acyl and double bond positions in phosphatidylcholines using a dual stage CID fragmentation coupled with ion mobility mass spectrometry
    Jose Castro-Perez
    Department of Atherosclerosis Exploratory Biomarkers, Merck Research Laboratories, 126 E Lincoln Ave, 80Y 2D7, Rahway, NJ 07065, USA
    J Am Soc Mass Spectrom 22:1552-67. 2011
    ..And was proven to be derived from the α-proximal (carboxylate) or distant ω-position (methyl) in the LPC...
  13. doi request reprint AAV8-mediated long-term expression of human LCAT significantly improves lipid profiles in hCETP;Ldlr(+/-) mice
    Zhu Chen
    Atherosclerosis, Cardiovascular Diseases, Merck Research Laboratories, RY80T A100, 126 E Lincoln Ave, Rahway, NJ 07065, USA
    J Cardiovasc Transl Res 4:801-10. 2011
    ..Our findings thus shed new light on LCAT's mechanism of action and lend support to its therapeutic potential in treating dyslipidemia...
  14. doi request reprint Enhanced data-independent analysis of lipids using ion mobility-TOFMSE to unravel quantitative and qualitative information in human plasma
    Vinit Shah
    Analytical Biochemistry and Molecular Biomarkers, Merck Research Laboratories, Kenilworth, NJ 07033, USA
    Rapid Commun Mass Spectrom 27:2195-200. 2013
    ....
  15. doi request reprint An ultraperformance liquid chromatography method for the normal-phase separation of lipids
    David G McLaren
    Atherosclerosis Research, Merck Research Laboratories, Merck and Co Inc, Rahway, NJ 07065, USA
    Anal Biochem 414:266-72. 2011
    ..6 to 10.5% CV with a variability in retention time of less than 6%. The utility of the method is demonstrated through the separation and quantitation of lipids in mouse plasma, liver, and heart tissue...
  16. doi request reprint An LC-MRM method for measuring intestinal triglyceride assembly using an oral stable isotope-labeled fat challenge
    Xiaofang Li
    Merck Research Laboratories, Merck and Co, Inc, Kenilworth, NJ, USA
    Bioanalysis 8:1265-77. 2016
    ..Stable isotope-labeled tracers combined with LC-MRM can be used to identify and distinguish TG synthesized with dietary and stored fatty acids...
  17. pmc Dose-dependent effects of siRNA-mediated inhibition of SCAP on PCSK9, LDLR, and plasma lipids in mouse and rhesus monkey
    Kristian K Jensen
    Cardiometabolic Disease Merck and Co Inc, Kenilworth, NJ
    J Lipid Res 57:2150-2162. 2016
    ..In conclusion, siRNA-mediated inhibition of SCAP resulted in a significant reduction in circulating PCSK9 and LDL-C in rodent and primate models supporting SCAP as a novel target for the treatment of dyslipidemia...
  18. pmc In vivo D2O labeling to quantify static and dynamic changes in cholesterol and cholesterol esters by high resolution LC/MS
    Jose Castro-Perez
    Atherosclerosis Exploratory Biomarkers Group, Merck and Co, Inc, Rahway, NJ, USA
    J Lipid Res 52:159-69. 2011
    ..We concluded that it is possible to readily obtain static and dynamic measurement of cholesterol and CEs in vivo by coupling novel LC/MS methods with stable isotope-based protocols...
  19. pmc Lipidome of atherosclerotic plaques from hypercholesterolemic rabbits
    Lazar A Bojic
    Merck Research Laboratories, 2000 Galloping Hill Road, Kenilworth, NJ 07033, USA
    Int J Mol Sci 15:23283-93. 2014
    ..The studies presented here are the first approach to a comprehensive characterization of the atherosclerotic plaque lipidome. ..
  20. doi request reprint Effects of anacetrapib on plasma lipids, apolipoproteins and PCSK9 in healthy, lean rhesus macaques
    Thomas P Roddy
    Merck Research Laboratories, Merck and Co, Inc 2000 Galloping Hill Road, Kenilworth, NJ 07033, USA
    Eur J Pharmacol 740:410-6. 2014
    ..Collectively, these data suggest that rhesus macaques may be a useful translational model to study the mechanistic effects of CETP inhibition...
  21. doi request reprint Pharmacological inhibition of diacylglycerol acyltransferase 1 reduces body weight and modulates gut peptide release--potential insight into mechanism of action
    Jinqi Liu
    Merck Research Laboratories, Rahway, New Jersey, USA
    Obesity (Silver Spring) 21:1406-15. 2013
    ..Investigation was conducted to understand the mechanism of action of diacylglycerol acyltransferase 1 (DGAT1) using small molecules DGAT1 inhibitors, compounds K and L...
  22. pmc Plasma lipid profiling across species for the identification of optimal animal models of human dyslipidemia
    Wu Yin
    Department of Atherosclerosis, Merck Research Laboratories, Rahway, NJ 07065, USA
    J Lipid Res 53:51-65. 2012
    ..Most traditional models, including rabbit, Zucker diabetic fatty rat, and the majority of mouse models, did not demonstrate overall similarity to dyslipidemic humans in this study...
  23. doi request reprint Identification of DGAT2 Inhibitors Using Mass Spectrometry
    Xuelei S Song
    Department of Pharmacology, Merck Research Laboratories, Boston, MA, USA
    J Biomol Screen 21:117-26. 2016
    ..Additional profiling of one chemotype in particular identified two promising reversible and selective compounds (compound 15 and compound 16) that effectively inhibit TG production in mouse primary hepatocytes. ..