Xujing Wang

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. pmc Investigating the role of islet cytoarchitecture in its oscillation using a new beta-cell cluster model
    Aparna Nittala
    Max McGee National Research Center for Juvenile Diabetes, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America
    PLoS ONE 2:e983. 2007
  2. pmc Quantitative measurement of pathogen-specific human memory T cell repertoire diversity using a CDR3 beta-specific microarray
    Xujing Wang
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, The Medical College of Wisconsin and The Children s Hospital Research Institute of Children s Hospital of Wisconsin, Milwaukee, WI 53226, USA
    BMC Genomics 8:329. 2007
  3. ncbi request reprint Evidence of a functional role for mast cells in the development of type 1 diabetes mellitus in the BioBreeding rat
    Rhonda Geoffrey
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics at the Medical College of Wisconsin, and Children s Research Institute of the Children s Hospital of Wisconsin, Milwaukee, WI 53226, USA
    J Immunol 177:7275-86. 2006
  4. pmc Comprehensive quality control utilizing the prehybridization third-dye image leads to accurate gene expression measurements by cDNA microarrays
    Xujing Wang
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, The Medical College of Wisconsin and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    BMC Bioinformatics 7:378. 2006
  5. ncbi request reprint Three-color cDNA microarrays with prehybridization quality control yield gene expression data comparable to that of commercial platforms
    Martin J Hessner
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, Medical College of Wisconsin, Children s Hospital Research Institute, Milwaukee, Wisconsin, USA
    Physiol Genomics 25:166-78. 2006
  6. pmc Identification of a serum-induced transcriptional signature associated with type 1 diabetes in the BioBreeding rat
    Mary Kaldunski
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics at the Medical College of Wisconsin, the Children s Research Institute of Children s Hospital of Wisconsin, and theHuman and Molecular Genetics Center, Milwaukee, Wisconsin, USA
    Diabetes 59:2375-85. 2010
  7. ncbi request reprint Identification of a molecular signature in human type 1 diabetes mellitus using serum and functional genomics
    Xujing Wang
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, Medical College of Wisconsin, Children s Hospital of Wisconsin, Milwaukee 53226, USA
    J Immunol 180:1929-37. 2008
  8. ncbi request reprint Quantitative quality control in microarray experiments and the application in data filtering, normalization and false positive rate prediction
    Xujing Wang
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, Medical College and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Bioinformatics 19:1341-7. 2003
  9. ncbi request reprint Involvement of eotaxin, eosinophils, and pancreatic predisposition in development of type 1 diabetes mellitus in the BioBreeding rat
    Martin J Hessner
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics at the Medical College of Wisconsin and the Children s Research Institute of the Children s Hospital of Wisconsin, Milwaukee, WI 53226, USA
    J Immunol 173:6993-7002. 2004
  10. pmc A joint association test for multiple SNPs in genetic case-control studies
    Tao Wang
    Division of Biostatistics, Department of Population Health, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 0509, USA
    Genet Epidemiol 33:151-63. 2009

Collaborators

Detail Information

Publications35

  1. pmc Investigating the role of islet cytoarchitecture in its oscillation using a new beta-cell cluster model
    Aparna Nittala
    Max McGee National Research Center for Juvenile Diabetes, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America
    PLoS ONE 2:e983. 2007
    ..These findings are important to understand the islet bursting phenomenon and the regulation of insulin secretion, under both physiological and pathological conditions...
  2. pmc Quantitative measurement of pathogen-specific human memory T cell repertoire diversity using a CDR3 beta-specific microarray
    Xujing Wang
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, The Medical College of Wisconsin and The Children s Hospital Research Institute of Children s Hospital of Wisconsin, Milwaukee, WI 53226, USA
    BMC Genomics 8:329. 2007
    ..We have used the memory T cell repertoire to an influenza-derived peptide as a test case for developing such a method...
  3. ncbi request reprint Evidence of a functional role for mast cells in the development of type 1 diabetes mellitus in the BioBreeding rat
    Rhonda Geoffrey
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics at the Medical College of Wisconsin, and Children s Research Institute of the Children s Hospital of Wisconsin, Milwaukee, WI 53226, USA
    J Immunol 177:7275-86. 2006
    ..05) delayed T1DM onset. These findings are consistent with a growing body of evidence in human and animal models, where a role for mast cells in the initiation and progression of autoimmune disease is emerging...
  4. pmc Comprehensive quality control utilizing the prehybridization third-dye image leads to accurate gene expression measurements by cDNA microarrays
    Xujing Wang
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, The Medical College of Wisconsin and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    BMC Bioinformatics 7:378. 2006
    ..Previously, we have reported a novel 3-color microarray technology that enabled array fabrication QC. In this report we further investigated its advantage in spot-level data QC...
  5. ncbi request reprint Three-color cDNA microarrays with prehybridization quality control yield gene expression data comparable to that of commercial platforms
    Martin J Hessner
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, Medical College of Wisconsin, Children s Hospital Research Institute, Milwaukee, Wisconsin, USA
    Physiol Genomics 25:166-78. 2006
    ....
  6. pmc Identification of a serum-induced transcriptional signature associated with type 1 diabetes in the BioBreeding rat
    Mary Kaldunski
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics at the Medical College of Wisconsin, the Children s Research Institute of Children s Hospital of Wisconsin, and theHuman and Molecular Genetics Center, Milwaukee, Wisconsin, USA
    Diabetes 59:2375-85. 2010
    ....
  7. ncbi request reprint Identification of a molecular signature in human type 1 diabetes mellitus using serum and functional genomics
    Xujing Wang
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, Medical College of Wisconsin, Children s Hospital of Wisconsin, Milwaukee 53226, USA
    J Immunol 180:1929-37. 2008
    ..Furthermore, proinflammatory serum markers may be used as inclusion criteria or endpoint measures in clinical trials aimed at preventing T1DM...
  8. ncbi request reprint Quantitative quality control in microarray experiments and the application in data filtering, normalization and false positive rate prediction
    Xujing Wang
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, Medical College and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Bioinformatics 19:1341-7. 2003
    ..5 for the replicate concordance rate is needed in order to be certain of true positives. Our work demonstrates the importance and advantages of having a quantitative quality control scheme for microarrays...
  9. ncbi request reprint Involvement of eotaxin, eosinophils, and pancreatic predisposition in development of type 1 diabetes mellitus in the BioBreeding rat
    Martin J Hessner
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics at the Medical College of Wisconsin and the Children s Research Institute of the Children s Hospital of Wisconsin, Milwaukee, WI 53226, USA
    J Immunol 173:6993-7002. 2004
    ..These data support an important role for these inflammatory mediators in BB rat T1DM and suggest that the lymphopenia due to the Ian5/(lyp) mutation may result in a deregulation of cells involved in insulitis and beta cell destruction...
  10. pmc A joint association test for multiple SNPs in genetic case-control studies
    Tao Wang
    Division of Biostatistics, Department of Population Health, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 0509, USA
    Genet Epidemiol 33:151-63. 2009
    ..Our simulation results also reveal that the latent variable approach may have improved power under certain scenarios comparing with classical haplotype association methods...
  11. pmc Apoptosis of CD4+ CD25(high) T cells in type 1 diabetes may be partially mediated by IL-2 deprivation
    Parthav Jailwala
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics at the Medical College of Wisconsin and the Children s Research Institute of the Children s Hospital of Wisconsin, Milwaukee, Wisconsin, United States of America
    PLoS ONE 4:e6527. 2009
    ..Our objective here was to elucidate the genes and signaling pathways triggering apoptosis in T(regs) from T1D subjects...
  12. doi request reprint Single-nucleotide polymorphisms in the IL2RA gene are associated with age at diagnosis in late-onset Finnish type 1 diabetes subjects
    Matthew W Klinker
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI, USA
    Immunogenetics 62:101-7. 2010
    ..These findings suggest that the IL2RA locus controls both the susceptibility to disease and its time of occurrence. Thus, we believe the IL2/IL2R axis represents a potential therapeutic target for delaying the onset of disease...
  13. pmc Use of transcriptional signatures induced in lymphoid and myeloid cell lines as an inflammatory biomarker in Type 1 diabetes
    Shuang Jia
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics at the Medical College of Wisconsin, and The Children s Research Institute of Children s Hospital of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Physiol Genomics 43:697-709. 2011
    ..However, the response of each is distinct, possessing varying degrees of functional relatedness to that observed with PBMC...
  14. pmc Utilization of a labeled tracking oligonucleotide for visualization and quality control of spotted 70-mer arrays
    Martin J Hessner
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, The Medical College of Wisconsin and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    BMC Genomics 5:12. 2004
    ....
  15. pmc Interleukin-1 antagonism moderates the inflammatory state associated with Type 1 diabetes during clinical trials conducted at disease onset
    Susanne M Cabrera
    The Max McGee National Research Center for Juvenile Diabetes, Children s Research Institute of Children s Hospital of Wisconsin, and Department of Pediatrics at the Medical College of Wisconsin, Milwaukee, WI, USA
    Eur J Immunol 46:1030-46. 2016
    ..Despite the absence of clinical efficacy by IL-1 antagonist therapy, transcriptional analysis detected immunomodulation and may yield new insight when applied to other clinical trials. ..
  16. doi request reprint Kinetics of insulin secretion to acute, repetitive stimulation of islets in vivo in Sprague Dawley rats
    Jianliang Chen
    Medical College of Wisconsin, Milwaukee, USA
    Islets 2:10-7. 2010
    ..The extension of this study to investigate insulin secretion in pre-diabetic states may be informative of the early pathogenic mechanisms...
  17. pmc Use of a three-color cDNA microarray platform to measure and control support-bound probe for improved data quality and reproducibility
    Martin J Hessner
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, The Medical College of Wisconsin and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Nucleic Acids Res 31:e60. 2003
    ..Collectively, this strategy will improve microarray data and increase its utility as a sensitive screening tool...
  18. ncbi request reprint Difference in gene expression profiles between human CD4+CD25+ and CD4+CD25- T cells
    Nirupma Pati
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, Medical College and Children s Hospital of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Ann N Y Acad Sci 1005:279-83. 2003
    ..Our preliminary results indicate that there are likely significant differences in the regulation of apoptosis, cell cycle, cytokine receptor, cell-cell interaction, and stress pathway genes between these two subtypes of T cells...
  19. pmc Immobilized probe and glass surface chemistry as variables in microarray fabrication
    Martin J Hessner
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, The Medical College of Wisconsin and Children s Hospital of Wisconsin, Milwaukee, WI 53226, USA
    BMC Genomics 5:53. 2004
    ....
  20. ncbi request reprint Transcriptional characterizations of differences between eutopic and ectopic endometrium
    Yan Wu
    Department of Pediatrics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 0509, USA
    Endocrinology 147:232-46. 2006
    ..The identification of these genes and their associated pathways provides new insight. Our findings will stimulate future investigations on molecular genetic mechanisms underlying the pathogenesis of endometriosis...
  21. pmc The hyperbolic effect of density and strength of inter beta-cell coupling on islet bursting: a theoretical investigation
    Aparna Nittala
    Max McGee National Research Center for Juvenile Diabetes and Human and Molecular Genetics Center, Medical College of Wisconsin and Children s Research Institute of the Children s Hospital of Wisconsin, Milwaukee, WI 53226, USA
    Theor Biol Med Model 5:17. 2008
    ..In this study, we further investigate two key structural measures: the number of neighboring cells that each beta-cell is coupled to, nc, and the coupling strength, gc...
  22. pmc Dimensional analysis of MINMOD leads to definition of the disposition index of glucose regulation and improved simulation algorithm
    Aparna Nittala
    Max McGee National Research Center for Juvenile Diabetes and Human and Molecular Genetics Center, Medical College of Wisconsin, Children s Research Institute, Children s Hospital of Wisconsin, Milwaukee, Wl 53226, USA
    Biomed Eng Online 5:44. 2006
    ..Dimensional analysis of the model is up to now not available...
  23. ncbi request reprint Meta-analysis of vitamin D receptor polymorphisms and type 1 diabetes: a HuGE review of genetic association studies
    Sun Wei Guo
    Department of Pediatrics and the Max McGee Research Center for Juvenile Diabetes, Medical College of Wisconsin, Children s Research Institute, Children s Hospital of Wisconsin, Milwaukee, 53226 0509, USA
    Am J Epidemiol 164:711-24. 2006
    ..In fact, a reanalysis of previously published data (McDermott et al., Diabetologia 1997;40:971-5) indicated no evidence of an association as reported...
  24. ncbi request reprint The design of a gene chip for functional immunological studies on a high-quality control platform
    Jill Waukau
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, Medical College and Children s Hospital of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Ann N Y Acad Sci 1005:284-7. 2003
    ..With Matarray, we have built a quantitative data filtering and normalization scheme that has proved to be more efficient than the existing methods. The list of immunology chip genes is available from the authors...
  25. ncbi request reprint A novel approach for high-quality microarray processing using third-dye array visualization technology
    Xujing Wang
    Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    IEEE Trans Nanobioscience 2:193-201. 2003
    ..Furthermore, we demonstrate use of the third-dye image to better quality control microarray image analysis. Both the principle and implementation of the approach are presented in detail, with experimental results...
  26. pmc TAPPA: topological analysis of pathway phenotype association
    Shouguo Gao
    The Max McGee National Research Center for Juvenile Diabetes and The Human and Molecular Genetics Center, The Medical College of Wisconsin and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Bioinformatics 23:3100-2. 2007
    ..TAPPA also allows evaluation of sub-modules within a pathway and their association to phenotypes...
  27. doi request reprint Microalterations of inherently unstable genomic regions in rat mammary carcinomas as revealed by long oligonucleotide array-based comparative genomic hybridization
    Tatjana Adamovic
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Cancer Res 69:5159-67. 2009
    ....
  28. pmc Membrane dielectric changes indicate induced apoptosis in HL-60 cells more sensitively than surface phosphatidylserine expression or DNA fragmentation
    Xujing Wang
    Department of Molecular Pathology, Section of Experimental Pathology, M D Anderson Cancer Center, The University of Texas, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Biochim Biophys Acta 1564:412-20. 2002
    ....
  29. pmc Haplotype analysis of the apolipoprotein gene cluster on human chromosome 11
    Michael Olivier
    Human and Molecular Genetics Center, Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Genomics 83:912-23. 2004
    ..These results highlight the complex genetic relationship between APOA5 and APOC3 and support the notion that APOA5 represents an independent risk gene affecting plasma triglyceride concentrations in humans...
  30. ncbi request reprint Conditions to ensure competitive hybridization in two-color microarray: a theoretical and experimental analysis
    Youmin Wang
    Department of Pediatrics, Medical College of Wisconsin, Children s Hospital of Wisconsin, Milwaukee 53226, USA
    Biotechniques 32:1342-6. 2002
    ..The method of validation is easily implemented to ensure data reliability by two-color microarray...
  31. pmc Three color cDNA microarrays: quantitative assessment through the use of fluorescein-labeled probes
    Martin J Hessner
    The Max McGee National Research Center for Juvenile Diabetes, The Medical College of Wisconsin and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Nucleic Acids Res 31:e14. 2003
    ..80, P < 0.001) was observed. We therefore conclude that slide selection based upon prehybridization quality scores will greatly benefit the ability to generate reliable gene expression data...
  32. pmc Molecular signatures differentiate immune states in type 1 diabetic families
    Yi Guang Chen
    The Max McGee National Research Center for Juvenile Diabetes, Children s Research Institute of Children s Hospital of Wisconsin, and Department of Pediatrics at the Medical College of Wisconsin, Milwaukee, WI
    Diabetes 63:3960-73. 2014
    ..In AA(-) HRS, the familial innate inflammatory state also was temporally supplanted by immunoregulatory processes, suggesting a mechanism underlying the decline in T1D susceptibility with age. ..
  33. pmc Increasing incidence of pediatric type 1 diabetes mellitus in Southeastern Wisconsin: relationship with body weight at diagnosis
    Jennifer Evertsen
    Department of Epidemiology, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America
    PLoS ONE 4:e6873. 2009
    ..Our objective was to assess whether changing incidence of type 1 diabetes in a group of children and adolescent from the Midwest United States was associated with changes in BMI...
  34. ncbi request reprint Investigation of the age-at-onset heterogeneity in type 1 diabetes through mathematical modeling
    Xujing Wang
    Max McGee National Research Center for Juvenile Diabetes and Human and Molecular Genetics Center, Medical College of Wisconsin and Children s Research Institute of the Children s Hospital of Wisconsin, Milwaukee, WI 53226, United States
    Math Biosci 203:79-99. 2006
    ..More importantly, the model analyses point out that pathways regulating beta-cell turnover can be new targets to interfere with the disease process of T1D...
  35. ncbi request reprint Leucine culture reveals that ATP synthase functions as a fuel sensor in pancreatic beta-cells
    Jichun Yang
    Department of Pathology and Laboratory Medicine, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 279:53915-23. 2004
    ..These data reveal the fuel-sensing role of mitochondrial ATP synthase in the control of ATP production from glucose and the control of glucose-induced insulin secretion...