Genomes and Genes
Aron M Geurts
Affiliation: Medical College of Wisconsin
- Progress and prospects in rat genetics: a community viewTimothy J Aitman
Medical Research Council Clinical Sciences Centre and Imperial College London, Du Cane Road, London W12 0NN, UK
Nat Genet 40:516-22. 2008..Finally, we present a set of principles that justify continuing and strengthening genetic studies in the rat model, and further development of genomic infrastructure for rat research...
- Maternal diet during gestation and lactation modifies the severity of salt-induced hypertension and renal injury in Dahl salt-sensitive ratsAron M Geurts
From the Departments of Physiology A M G, D L M, P L, M M S, T M K, C Y, B T E, J K, M L, A W C, Pediatrics E C, and Cardiovascular Research Center A M G, Medical College of Wisconsin, Milwaukee
Hypertension 65:447-55. 2015..Together, the data suggest that maternal diet during the gestational-lactational period has substantial effects on the development of salt-induced hypertension and renal injury in adult SS rats. ..
- Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposonAron M Geurts
Department of Genetics, Cell Biology, and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
BMC Biotechnol 6:30. 2006..We sought to generate a transposon system that would be capable of reporting the expression pattern of a mouse gene while allowing for conditional expression of a gene of interest in a tissue- or temporal-specific pattern...
- Zinc-finger nucleases: new strategies to target the rat genomeAron M Geurts
Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, 53226, USA
Clin Sci (Lond) 119:303-11. 2010..The ability to generate knockout rat models using zinc-finger nuclease technology will now enable its full emergence as an exceptional physiological and genetic research model...
- Identifying multiple causative genes at a single GWAS locusMichael J Flister
Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
Genome Res 23:1996-2002. 2013..Combined, these data demonstrate for the first time that multiple modifiers of hypertension can cosegregate at a single GWAS locus. ..
- SH2B3 Is a Genetic Determinant of Cardiac Inflammation and FibrosisMichael J Flister
From the Human and Molecular Genetics Center M J F, M J H, A L, S Z P, S W T, A M G, J L, H J J, Departments of Physiology M J F, M J H, A L, S Z P, N R, A M G, H J J, Dermatology J L, and Pediatrics H J J, Medical College of Wisconsin, Milwaukee Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA C C O M Department of Cardiovascular and Metabolic Disease at MedImmune, Cambridge, United Kingdom C M and Lillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis N A, J L H
Circ Cardiovasc Genet 8:294-304. 2015....
- Transposon vectors for gene-trap insertional mutagenesis in vertebratesKarl J Clark
Department of Genetics, Cell Biology, and Development, Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Minneapolis, Minnesota, USA
Genesis 39:225-33. 2004....
- Structure-based prediction of insertion-site preferences of transposons into chromosomesAron M Geurts
Department of Genetics, Cell Biology and Development, The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
Nucleic Acids Res 34:2803-11. 2006..ProTIS analysis can be useful for functional genomic, gene transfer and human gene therapy applications using the SB system...
- Efficient mammalian germline transgenesis by cis-enhanced Sleeping Beauty transpositionDaniel F Carlson
The Center for Genome Engineering, Minneapolis, MN, USA
Transgenic Res 20:29-45. 2011..Given its simplicity, versatility and high efficiency, TnT with enhanced Sleeping Beauty components represents a compelling non-viral approach to modifying the mammalian germline...
- Pappa2 is linked to salt-sensitive hypertension in Dahl S ratsAllen W Cowley
Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin
Physiol Genomics 48:62-72. 2016..We conclude that we have identified a variant form of Pappa2 that can protect against salt-induced hypertension in the Dahl S rat. ..
- Increased expression of NAD(P)H oxidase subunit p67(phox) in the renal medulla contributes to excess oxidative stress and salt-sensitive hypertensionDi Feng
Physiology Department, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Cell Metab 15:201-8. 2012..These rats exhibited a significant reduction of salt-sensitive hypertension and renal medullary oxidative stress and injury. p67(phox) could represent a target for salt-sensitive hypertension therapy...
- CD247 modulates blood pressure by altering T-lymphocyte infiltration in the kidneyNathan Rudemiller
Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226
Hypertension 63:559-64. 2014....
- Gene mutations and genomic rearrangements in the mouse as a result of transposon mobilization from chromosomal concatemersAron M Geurts
Department of Genetics, Cell Biology, and Development, University of Minnesota Twin Cities, Minneapolis, Minnesota, United States of America
PLoS Genet 2:e156. 2006..Additionally, these mice may serve as a model for chromosome rearrangements caused by transposable elements during the evolution of vertebrate genomes...
- Target-site preferences of Sleeping Beauty transposonsGeyi Liu
Department of Genetics, Cell Biology, and Development and The Arnold and Mabel Beckman Center for Transposon Research at the University of Minnesota, Minneapolis, MN 55455, USA
J Mol Biol 346:161-73. 2005..Our observations suggest that particular deformations of the double helix predicted by the V(step) algorithm can distinguish TA sites that vary by about 16-fold in their preferences for accommodating insertions of SB transposons...
- Genetic mutation of recombination activating gene 1 in Dahl salt-sensitive rats attenuates hypertension and renal damageDavid L Mattson
Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
Am J Physiol Regul Integr Comp Physiol 304:R407-14. 2013..These studies demonstrate the importance of renal infiltration of immune cells in the pathogenesis of hypertension and renal damage in Dahl SS rats...
- Creation and characterization of a renin knockout ratCarol Moreno
Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Hypertension 57:614-9. 2011....
- Generation of gene-specific mutated rats using zinc-finger nucleasesAron M Geurts
Human and Molecular Genetics Center, Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA
Methods Mol Biol 597:211-25. 2010..This technology allows for the rapid, targeted modification of the rat genome...
- Mutation of SH2B3 (LNK), a genome-wide association study candidate for hypertension, attenuates Dahl salt-sensitive hypertension via inflammatory modulationNathan P Rudemiller
From the Department of Physiology N P R, H L, J R C P, B T E, J W P, H J J, A M G, D L M, and Human and Molecular Genetics Center B T E, J W P, H J J, A M G, Medical College of Wisconsin, Milwaukee and Department of Medicine, Medical College of Wisconsin, Zablocki Veterans Affairs Medical Center, Milwaukee E P C
Hypertension 65:1111-7. 2015..These data further support a role for the immune system as a modulator of disease severity in the pathogenesis of hypertension and provide insight into inflammatory mechanisms at play in human hypertension and renal disease. ..
- CXM: a new tool for mapping breast cancer risk in the tumor microenvironmentMichael J Flister
Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, Wisconsin Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin
Cancer Res 74:6419-29. 2014..Collectively, our results establish the utility of CXM to localize genetic variants affecting the tumor microenvironment that underlie differences in breast cancer risk...
- Gene transfer into genomes of human cells by the sleeping beauty transposon systemAron M Geurts
Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, Minnesota 55455, USA
Mol Ther 8:108-17. 2003..With the recently described improvements to the transposon itself, the SB system appears to be a potential gene-transfer tool for human gene therapy...
- Knockout rats via embryo microinjection of zinc-finger nucleasesAron M Geurts
Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 52336, USA
Science 325:433. 2009..Our data demonstrate the feasibility of targeted gene disruption in multiple rat strains within 4 months time, paving the way to a humanized monoclonal antibody platform and additional human disease models...
- The NRF2 knockout rat: a new animal model to study endothelial dysfunction, oxidant stress, and microvascular rarefactionJessica R C Priestley
Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin and
Am J Physiol Heart Circ Physiol 310:H478-87. 2016....
- Efficient non-viral integration and stable gene expression in multipotent adult progenitor cellsAndrew Wilber
Center for Genome Engineering, Gene Therapy Program, Institute of Human Genetics, University of Minnesota, Minneapolis, MN 55455, USA
Stem Cells Int 2011:717069. 2011..We conclude that both SB and ϕC31 are effective non-viral integrating systems for genetic engineering of MAPC in basic studies of stem cell biology...
- Gene targeting in the rat: advances and opportunitiesHoward J Jacob
Department of Dermatology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
Trends Genet 26:510-8. 2010..Here, we provide a high-level description and the potential uses of these new technologies for investigators using the rat for biomedical research...
- Amelioration of salt-induced vascular dysfunction in mesenteric arteries of Dahl salt-sensitive rats by missense mutation of extracellular superoxide dismutaseAndreas M Beyer
Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin
Am J Physiol Heart Circ Physiol 306:H339-47. 2014....
- The emerging role for rat models in gene discoveryMelinda R Dwinell
Department of Physiology, Medical College of Wisconsin, Milwaukee, 53226, USA
Mamm Genome 22:466-75. 2011..The strategies used to translate disease traits to genes to function, and, ultimately, to improve human health is discussed. Finally, our perspective on how rat models will continue to positively impact biomedical research is provided...
- Efficient transgenic rat production by a lentiviral vectorMieczyslaw Michalkiewicz
Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
Am J Physiol Heart Circ Physiol 293:H881-94. 2007..Thus lentiviral transgenesis provides a powerful tool for the production of transgenic inbred rats and will enhance the usefulness of this species in gene discovery and target validation studies...
- Characterization of Dahl salt-sensitive rats with genetic disruption of the A2B adenosine receptor gene: implications for A2B adenosine receptor signaling during hypertensionShraddha Nayak
Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA
Purinergic Signal 11:519-31. 2015..Our data indicate varying roles for A(2B)AR signaling in regulating blood pressure in SS rats, playing both anti- and prohypertensive roles depending on the pathogenic mechanisms that contribute to blood pressure elevation...
- Gene targeting in NOD mouse embryos using zinc-finger nucleasesYi Guang Chen
Max McGee National Research Center for Juvenile Diabetes, Medical College of Wisconsin, Milwaukee, WI
Diabetes 63:68-74. 2014..Our study demonstrates the ability to generate gene-targeted knockouts in a pure NOD background by using ZFNs without potential confounding factors introduced by contaminating genetic materials obtained from other strains. ..
- Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon systemBaisong Lu
Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas 77030, USA
Mamm Genome 18:338-46. 2007..These data suggest that the modified Sleeping Beauty transposon represents a powerful new tool for producing molecularly defined mutagenesis in the rat...
- Predicting preferential DNA vector insertion sites: implications for functional genomics and gene therapyChristopher S Hackett
Biomedical Sciences Graduate Program and Department of Neurology, University of California San Francisco, Room U441K, Parnassus Ave, San Francisco, California 94143 0663, USA
Genome Biol 8:S12. 2007....
- Structure-function analysis of the inverted terminal repeats of the sleeping beauty transposonZongbin Cui
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, People s Republic of China
J Mol Biol 318:1221-35. 2002..These findings have led to the construction of a more advanced transposon that should be useful in gene transfer and insertional mutagenesis in vertebrates...