Timothy K Lu

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. pmc Stretchable living materials and devices with hydrogel-elastomer hybrids hosting programmed cells.
    Xinyue Liu
    Proc Natl Acad Sci U S A 114:2200-2205. 2017
  2. pmc Tunable and multifunctional eukaryotic transcription factors based on CRISPR/Cas
    Fahim Farzadfard
    Department of Electrical Engineering and Computer Science and Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
    ACS Synth Biol 2:604-13. 2013
  3. pmc Next-generation synthetic gene networks
    Timothy K Lu
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Biotechnol 27:1139-50. 2009
  4. pmc Engineering scalable biological systems
    Timothy K Lu
    Synthetic Biology Group, Research Lab of Electronics, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
    Bioeng Bugs 1:378-84. 2010
  5. doi request reprint The next generation of bacteriophage therapy
    Timothy K Lu
    Synthetic Biology Group, Research Laboratory of Electronics, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
    Curr Opin Microbiol 14:524-31. 2011
  6. pmc Synthesis and patterning of tunable multiscale materials with engineered cells
    Allen Y Chen
    1 Biophysics Program, Harvard University, Cambridge, Massachusetts 02138, USA 2 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, USA 3 Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, USA 4 MIT Synthetic Biology Center, 500 Technology Square Cambridge, Massachusetts 02139, USA 5 Harvard MIT Health Sciences and Technology, Institute for Medical Engineering and Science, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, USA
    Nat Mater 13:515-23. 2014
  7. pmc Engineered bacteriophage targeting gene networks as adjuvants for antibiotic therapy
    Timothy K Lu
    Harvard Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 106:4629-34. 2009
  8. pmc Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care
    Pablo Perez-Pinera
    Synthetic Biology Group, Department of Biological Engineering and Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nat Commun 7:12211. 2016
  9. doi request reprint Continuous genetic recording with self-targeting CRISPR-Cas in human cells
    Samuel D Perli
    Synthetic Biology Group, MIT Synthetic Biology Center, Massachusetts Institute of Technology MIT, Cambridge, MA 02139, USA Research Laboratory of Electronics, MIT, Cambridge, MA 02139, USA Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA
    Science 353:. 2016
  10. pmc Microbiome therapeutics - Advances and challenges
    Mark Mimee
    MIT Microbiology Program, 77 Massachusetts Avenue, Cambridge, MA, USA MIT Synthetic Biology Center, 500 Technology Square, Cambridge, MA, USA The Center for Microbiome Informatics and Therapeutics, Cambridge, MA, USA
    Adv Drug Deliv Rev 105:44-54. 2016

Collaborators

  • Zhengtao Deng
  • Peter A Carr
  • Volker F Wendisch
  • Ernest Fraenkel
  • Collin M Stultz
  • Rahul Sarpeshkar
  • Omid C Farokhzad
  • Bijan Zakeri
  • Samuel D Perli
  • Mark Mimee
  • Robert J Citorik
  • Allen A Cheng
  • Sara Cleto
  • Alan S L Wong
  • Pablo Perez-Pinera
  • Piro Siuti
  • Diana P Pires
  • Jacob R Rubens
  • Xinyue Liu
  • Barbara Jusiak
  • Cheryl H Cui
  • Kevin C Ma
  • Gigi C G Choi
  • Oliver Purcell
  • Hiroki Ando
  • Lior Nissim
  • Chao Zhong
  • Allen Y Chen
  • John Yazbek
  • Ramiz Daniel
  • Fahim Farzadfard
  • Aleksandar F Radovic-Moreno
  • El onore Tham
  • Shaoting Lin
  • Tzu Chieh Tang
  • Hyunwoo Yuk
  • Xuanhe Zhao
  • Ningren Han
  • Joana Azeredo
  • Pamela Milani
  • Samuel W Kazer
  • Jaide Vk Jensen
  • Jicong Cao
  • Kartik A Shah
  • Mario Hermann
  • Kevin Lee
  • Sanna Sillankorva
  • Gianluca Selvaggio
  • Rajeev Ram
  • Alex K Shalek
  • Gabriela Pregernig
  • Miriam Adam
  • Aleth Gaillard
  • Alex C Tucker
  • Sebastien Lemire
  • Christopher A Voigt
  • Thomas Gurry
  • Michelle Y Lu
  • Jordan Downey
  • Alexandra Fridkin
  • Urartu O S Seker
  • Amanda N Billings
  • Huiming Ding
  • Vlad A Puscasu
  • Christopher J Yoon
  • Robert Langer

Detail Information

Publications33

  1. pmc Stretchable living materials and devices with hydrogel-elastomer hybrids hosting programmed cells.
    Xinyue Liu
    Proc Natl Acad Sci U S A 114:2200-2205. 2017
    ..We further develop a quantitative model that couples transportation of signaling molecules and cellular response to aid the design of future living materials and devices...
  2. pmc Tunable and multifunctional eukaryotic transcription factors based on CRISPR/Cas
    Fahim Farzadfard
    Department of Electrical Engineering and Computer Science and Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
    ACS Synth Biol 2:604-13. 2013
    ....
  3. pmc Next-generation synthetic gene networks
    Timothy K Lu
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Biotechnol 27:1139-50. 2009
    ..As these challenges are addressed, synthetic biologists will be able to construct useful next-generation synthetic gene networks with real-world applications in medicine, biotechnology, bioremediation and bioenergy...
  4. pmc Engineering scalable biological systems
    Timothy K Lu
    Synthetic Biology Group, Research Lab of Electronics, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
    Bioeng Bugs 1:378-84. 2010
    ....
  5. doi request reprint The next generation of bacteriophage therapy
    Timothy K Lu
    Synthetic Biology Group, Research Laboratory of Electronics, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
    Curr Opin Microbiol 14:524-31. 2011
    ..These research efforts must be coupled with practical and rigorous approaches at academic, commercial, and regulatory levels in order to successfully advance bacteriophage therapy into clinical settings...
  6. pmc Synthesis and patterning of tunable multiscale materials with engineered cells
    Allen Y Chen
    1 Biophysics Program, Harvard University, Cambridge, Massachusetts 02138, USA 2 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, USA 3 Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, USA 4 MIT Synthetic Biology Center, 500 Technology Square Cambridge, Massachusetts 02139, USA 5 Harvard MIT Health Sciences and Technology, Institute for Medical Engineering and Science, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, USA
    Nat Mater 13:515-23. 2014
    ..This work lays a foundation for synthesizing, patterning, and controlling functional composite materials with engineered cells...
  7. pmc Engineered bacteriophage targeting gene networks as adjuvants for antibiotic therapy
    Timothy K Lu
    Harvard Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 106:4629-34. 2009
    ..This work establishes a synthetic biology platform for the rapid translation and integration of identified targets into effective antibiotic adjuvants...
  8. pmc Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care
    Pablo Perez-Pinera
    Synthetic Biology Group, Department of Biological Engineering and Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nat Commun 7:12211. 2016
    ....
  9. doi request reprint Continuous genetic recording with self-targeting CRISPR-Cas in human cells
    Samuel D Perli
    Synthetic Biology Group, MIT Synthetic Biology Center, Massachusetts Institute of Technology MIT, Cambridge, MA 02139, USA Research Laboratory of Electronics, MIT, Cambridge, MA 02139, USA Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA
    Science 353:. 2016
    ..This tool, Mammalian Synthetic Cellular Recorder Integrating Biological Events (mSCRIBE), provides a distinct strategy for investigating cell biology in vivo and enables continuous evolution of targeted DNA sequences. ..
  10. pmc Microbiome therapeutics - Advances and challenges
    Mark Mimee
    MIT Microbiology Program, 77 Massachusetts Avenue, Cambridge, MA, USA MIT Synthetic Biology Center, 500 Technology Square, Cambridge, MA, USA The Center for Microbiome Informatics and Therapeutics, Cambridge, MA, USA
    Adv Drug Deliv Rev 105:44-54. 2016
    ....
  11. pmc Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM
    Alan S L Wong
    Synthetic Biology Group, Massachusetts Institute of Technology MIT Synthetic Biology Center, MIT, Cambridge, MA 02139 Research Laboratory of Electronics, MIT, Cambridge, MA 02139
    Proc Natl Acad Sci U S A 113:2544-9. 2016
    ....
  12. pmc Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases
    Robert J Citorik
    1 MIT Microbiology Program, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA 2 MIT Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Biotechnol 32:1141-5. 2014
    ....
  13. pmc Strong underwater adhesives made by self-assembling multi-protein nanofibres
    Chao Zhong
    1 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA 2 Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA 3 Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA
    Nat Nanotechnol 9:858-66. 2014
    ..Moreover, they outperform Mfps or curli fibres taken on their own and exhibit better tolerance to auto-oxidation than Mfps at pH ≥ 7.0. ..
  14. pmc Synthetic biology of antimicrobial discovery
    Bijan Zakeri
    Synthetic Biology Group, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
    ACS Synth Biol 2:358-72. 2013
    ..Herein, we discuss the biological engineering of small molecules, peptides, and non-traditional antimicrobials and provide an overview of the growing applicability of synthetic biology to antimicrobials discovery. ..
  15. doi request reprint Engineering genetic circuits that compute and remember
    Piro Siuti
    1 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA 2 Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA 3 Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA 4
    Nat Protoc 9:1292-300. 2014
    ..It should enable the encoding of advanced computational operations in living cells, including sequential-logic and biological-state machines, for a broad range of applications in biotechnology, basic science and biosensing. ..
  16. doi request reprint Synthetic circuits integrating logic and memory in living cells
    Piro Siuti
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Biotechnol 31:448-52. 2013
    ..We envision that this integrated logic and memory system will enable the implementation of complex cellular state machines, behaviors and pathways for therapeutic, diagnostic and basic science applications...
  17. doi request reprint Synthetic biology: an emerging engineering discipline
    Allen A Cheng
    Synthetic Biology Group, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Annu Rev Biomed Eng 14:155-78. 2012
    ..This review aims to discuss the progress and challenges in synthetic biology and to illustrate areas where synthetic biology may impact biomedical engineering and human health...
  18. pmc Surface charge-switching polymeric nanoparticles for bacterial cell wall-targeted delivery of antibiotics
    Aleksandar F Radovic-Moreno
    Harvard MIT Division of Health Sciences and Technology, Cambridge, Massachusetts 02139, USA
    ACS Nano 6:4279-87. 2012
    ..The PLGA-PLH-PEG NPs described herein are a first step toward developing systemically administered drug carriers that can target and potentially treat Gram-positive, Gram-negative, or polymicrobial infections associated with acidity...
  19. pmc Genetically Engineered Phages: a Review of Advances over the Last Decade
    Diana P Pires
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA Centre of Biological Engineering, University of Minho, Braga, Portugal
    Microbiol Mol Biol Rev 80:523-43. 2016
    ..This review highlights advances in techniques used to engineer phages for all of these purposes and discusses existing challenges and opportunities for future work. ..
  20. pmc Multiplexed Sequence Encoding: A Framework for DNA Communication
    Bijan Zakeri
    Department of Electrical Engineering and Computer Science, Department of Biological Engineering, Research Laboratory of Electronics, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States of America
    PLoS ONE 11:e0152774. 2016
    ..We envision these approaches will enable more widespread communication of information via DNA. ..
  21. pmc Engineering Modular Viral Scaffolds for Targeted Bacterial Population Editing
    Hiroki Ando
    Department of Electrical Engineering and Computer Science and Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Cell Syst 1:187-196. 2015
    ..We envision that this approach will accelerate phage-biology studies and enable new technologies for bacterial population editing...
  22. pmc Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota
    Mark Mimee
    Department of Biological Engineering, Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, MA 02139, USA MIT Microbiology Program, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell Syst 1:62-71. 2015
    ..thetaiotaomicron colonizing the mouse gut. These results provide a blueprint for engineering new chassis and a resource to engineer Bacteroides for surveillance of or therapeutic delivery to the gut microbiome...
  23. pmc Synthetic mixed-signal computation in living cells
    Jacob R Rubens
    Synthetic Biology Group, MIT Synthetic Biology Center, Research Laboratory of Electronics, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Commun 7:11658. 2016
    ..We expect that this hybrid computational paradigm will enable new industrial, diagnostic and therapeutic applications with engineered cells. ..
  24. pmc Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi)
    Sara Cleto
    Department of Electrical Engineering and Computer Science and Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
    ACS Synth Biol 5:375-85. 2016
    ..glutamicum metabolic engineering, which only requires 3 days, indicates that CRISPRi can be used for quick and efficient metabolic pathway remodeling without the need for gene deletions or mutations and subsequent selection. ..
  25. doi request reprint Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology
    Barbara Jusiak
    Research Laboratory of Electronics, Synthetic Biology Center, Department of Biological Engineering and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
    Trends Biotechnol 34:535-47. 2016
    ....
  26. doi request reprint Foundations and Emerging Paradigms for Computing in Living Cells
    Kevin C Ma
    Synthetic Biology Group, Research Laboratory of Electronics, Department of Biological Engineering and Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    J Mol Biol 428:893-915. 2016
    ..Finally, we discuss notable challenges that the field of synthetic biology still faces in achieving reliable and predictable forward-engineering of artificial biological circuits. ..
  27. pmc Massively parallel high-order combinatorial genetics in human cells
    Alan S L Wong
    Synthetic Biology Group, MIT Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Biotechnol 33:952-61. 2015
    ..More broadly, our method will enable high-throughput profiling of multifactorial genetic combinations that regulate phenotypes of relevance to biomedicine, biotechnology and basic science. ..
  28. pmc DNA nanotechnology: new adventures for an old warhorse
    Bijan Zakeri
    Department of Electrical Engineering and Computer Science, Department of Biological Engineering, Research Laboratory of Electronics, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA MIT Synthetic Biology Center, 500 Technology Square, Cambridge, MA 02139, USA Electronic address
    Curr Opin Chem Biol 28:9-14. 2015
    ..Early studies of DNA as an alternative to magnetic and optical storage mediums have not only been promising, but have demonstrated the potential of DNA to revolutionize the way we interact with digital data in the future. ..
  29. pmc Enhanced killing of antibiotic-resistant bacteria enabled by massively parallel combinatorial genetics
    Allen A Cheng
    Synthetic Biology Group, MIT Synthetic Biology Center, Research Lab of Electronics, Departments of Electrical Engineering and Computer Science and Biological Engineering, Medical Engineering and Medical Physics Program, Harvard MIT Health Sciences and Technology, Harvard Biophysics Program and Harvard MD PhD Program, Harvard University, Boston, MA 02115
    Proc Natl Acad Sci U S A 111:12462-7. 2014
    ....
  30. pmc Bacteriophage-based synthetic biology for the study of infectious diseases
    Robert J Citorik
    MIT Microbiology Program, 77 Massachusetts Avenue, Cambridge, MA 02139, USA MIT Synthetic Biology Center, 500 Technology Square, Cambridge, MA 02139, USA
    Curr Opin Microbiol 19:59-69. 2014
    ..Though almost a century has elapsed since their discovery, bacteriophages continue to have a major impact on modern biological sciences, especially with the growth of multidrug-resistant bacteria and interest in the microbiome. ..
  31. pmc Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells
    Lior Nissim
    Synthetic Biology Group, Research Laboratory of Electronics, Department of Biological Engineering and Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Mol Cell 54:698-710. 2014
    ..This toolkit can be used for programming scalable gene circuits and perturbing endogenous networks for biology, therapeutic, and synthetic biology applications. ..
  32. doi request reprint Synthetic analog computation in living cells
    Ramiz Daniel
    Analog Circuits and Biological Systems Group, Research Lab of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 497:619-23. 2013
    ..Such circuits may lead to new applications for synthetic biology and biotechnology that require complex computations with limited parts, need wide-dynamic-range biosensing or would benefit from the fine control of gene expression...
  33. pmc Dispersing biofilms with engineered enzymatic bacteriophage
    Timothy K Lu
    Harvard MIT Division of Health Sciences and Technology, 77 Massachusetts Avenue, Room E25 519, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 104:11197-202. 2007
    ..This work demonstrates the feasibility and benefits of using engineered enzymatic bacteriophage to reduce bacterial biofilms and the applicability of synthetic biology to an important medical and industrial problem...