Raju Kucherlapati

Summary

Affiliation: Massachusetts General Hospital
Country: USA

Publications

  1. doi Genetically modified mouse models for biomarker discovery and preclinical drug testing
    Raju Kucherlapati
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Clin Cancer Res 18:625-30. 2012
  2. pmc A mouse plasma peptide atlas as a resource for disease proteomics
    Qing Zhang
    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Genome Biol 9:R93. 2008
  3. pmc Genetic mechanisms in Apc-mediated mammary tumorigenesis
    Mari Kuraguchi
    Harvard Partners Center for Genetics and Genomics, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Genet 5:e1000367. 2009
  4. pmc An Msh2 conditional knockout mouse for studying intestinal cancer and testing anticancer agents
    Melanie H Kucherlapati
    Department of Medicine Division of Genetics, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Gastroenterology 138:993-1002.e1. 2010
  5. pmc Loss of Rb1 in the gastrointestinal tract of Apc1638N mice promotes tumors of the cecum and proximal colon
    Melanie H Kucherlapati
    Harvard Partners Center for Genetics and Genomics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:15493-8. 2008
  6. pmc Spectrum of somatic mitochondrial mutations in five cancers
    Tatianna C Larman
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 109:14087-91. 2012
  7. pmc Development of a mouse model for sporadic and metastatic colon tumors and its use in assessing drug treatment
    Kenneth E Hung
    Division of Gastroenterology, Tufts Medical Center, Boston, MA 02111, USA
    Proc Natl Acad Sci U S A 107:1565-70. 2010
  8. pmc Adenomatous polyposis coli (APC) is required for normal development of skin and thymus
    Mari Kuraguchi
    Harvard Partners Center for Genetics and Genomics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 2:e146. 2006
  9. doi PolyPhred analysis software for mutation detection from fluorescence-based sequence data
    Kate T Montgomery
    Harvard Medical School Partners Healthcare Center for Genetics and Genomics, Boston, Massachusetts, USA
    Curr Protoc Hum Genet . 2008
  10. pmc Comprehensive proteome analysis of an Apc mouse model uncovers proteins associated with intestinal tumorigenesis
    Kenneth E Hung
    Department of Medicine, Tufts Medical Center, Boston, Massachusetts, USA
    Cancer Prev Res (Phila) 2:224-33. 2009

Collaborators

Detail Information

Publications50

  1. doi Genetically modified mouse models for biomarker discovery and preclinical drug testing
    Raju Kucherlapati
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Clin Cancer Res 18:625-30. 2012
    ..The ability to generate tumors at the correct anatomical site within the normal cellular environment is augmenting the use of xenografts in drug testing in a preclinical setting...
  2. pmc A mouse plasma peptide atlas as a resource for disease proteomics
    Qing Zhang
    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Genome Biol 9:R93. 2008
    ..A major finding from this study is the identification of novel isoforms and transcript variants not previously predicted from genome analysis...
  3. pmc Genetic mechanisms in Apc-mediated mammary tumorigenesis
    Mari Kuraguchi
    Harvard Partners Center for Genetics and Genomics, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Genet 5:e1000367. 2009
    ....
  4. pmc An Msh2 conditional knockout mouse for studying intestinal cancer and testing anticancer agents
    Melanie H Kucherlapati
    Department of Medicine Division of Genetics, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Gastroenterology 138:993-1002.e1. 2010
    ..Mutations in the DNA mismatch repair (MMR) gene MSH2 cause Lynch syndromes I and II and sporadic colorectal cancers. Msh2(null) mice predominantly develop lymphoma and do not accurately recapitulate the colorectal cancer phenotype...
  5. pmc Loss of Rb1 in the gastrointestinal tract of Apc1638N mice promotes tumors of the cecum and proximal colon
    Melanie H Kucherlapati
    Harvard Partners Center for Genetics and Genomics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:15493-8. 2008
    ..Expression profile analysis indicates the two tumor types differentially regulate distinct sets of genes that are over-expressed in a majority of human colorectal carcinomas...
  6. pmc Spectrum of somatic mitochondrial mutations in five cancers
    Tatianna C Larman
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 109:14087-91. 2012
    ..In summary, these data suggest that damaging somatic mtDNA mutations occur frequently (13-63%) in these five tumor types and likely confer a selective advantage in oncogenesis...
  7. pmc Development of a mouse model for sporadic and metastatic colon tumors and its use in assessing drug treatment
    Kenneth E Hung
    Division of Gastroenterology, Tufts Medical Center, Boston, MA 02111, USA
    Proc Natl Acad Sci U S A 107:1565-70. 2010
    ..These studies suggest that mTOR inhibitors should be further explored as potential colorectal cancer therapies in patients whose tumors do not have activating mutations in KRAS...
  8. pmc Adenomatous polyposis coli (APC) is required for normal development of skin and thymus
    Mari Kuraguchi
    Harvard Partners Center for Genetics and Genomics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 2:e146. 2006
    ....
  9. doi PolyPhred analysis software for mutation detection from fluorescence-based sequence data
    Kate T Montgomery
    Harvard Medical School Partners Healthcare Center for Genetics and Genomics, Boston, Massachusetts, USA
    Curr Protoc Hum Genet . 2008
    ..The software is being continually updated to permit further automation of mutation analysis. Currently, however, at least some manual review is required if one wishes to identify 100% of the variants in a sample set...
  10. pmc Comprehensive proteome analysis of an Apc mouse model uncovers proteins associated with intestinal tumorigenesis
    Kenneth E Hung
    Department of Medicine, Tufts Medical Center, Boston, Massachusetts, USA
    Cancer Prev Res (Phila) 2:224-33. 2009
    ..Our results show that integrated analysis of the plasma proteome and tumor transcriptome of genetically engineered mouse models is a powerful approach for the identification of tumor-related plasma proteins...
  11. pmc Lack of lymphatic vessel phenotype in LYVE-1/CD44 double knockout mice
    Mai X Luong
    Department of Radiation Oncology, Massachusetts General Hospital, Charlestown, Massachusetts, USA
    J Cell Physiol 219:430-7. 2009
    ..These data suggest that LYVE-1 and CD44 are not required for the formation or function of lymphatics, but do not rule out a role for LYVE-1 in inflammation...
  12. pmc Diverse mechanisms of somatic structural variations in human cancer genomes
    Lixing Yang
    Center for Biomedical Informatics, Harvard Medical School, Boston, MA 02115, USA
    Cell 153:919-29. 2013
    ....
  13. pmc Filter-based hybridization capture of subgenomes enables resequencing and copy-number detection
    Daniel S Herman
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    Nat Methods 6:507-10. 2009
    ..8% of targeted nucleotides >or=20 times ( approximately 40,000-fold enrichment), enabling sensitive and specific detection of sequence variation and copy-number variation...
  14. pmc COMT genetic variation confers risk for psychotic and affective disorders: a case control study
    Birgit Funke
    Harvard Partners Center for Genetics and Genomics, Boston, USA
    Behav Brain Funct 1:19. 2005
    ..Cases included patients with schizophrenia (n = 196), schizoaffective disorder (n = 62), bipolar disorder (n = 82), major depression (n = 30), and patients diagnosed with either psychotic disorder NOS or depressive disorder NOS (n = 24)...
  15. pmc Apc inhibition of Wnt signaling regulates supernumerary tooth formation during embryogenesis and throughout adulthood
    Xiu Ping Wang
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Development 136:1939-49. 2009
    ..In addition, we identify Fgf8, a known tooth initiation marker, as a direct target of Wnt/beta-catenin signaling. These studies identify key mechanistic features responsible for supernumerary tooth formation...
  16. pmc Activation of multiple signaling pathways causes developmental defects in mice with a Noonan syndrome‚Äďassociated Sos1 mutation
    Peng Chieh Chen
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 120:4353-65. 2010
    ....
  17. pmc In vivo optical molecular imaging of matrix metalloproteinase activity following celecoxib therapy for colorectal cancer
    Rahul A Sheth
    Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Massachusetts General Hospital, Boston, MA 02114, USA
    Mol Imaging 11:417-25. 2012
    ..This method may provide for the improved identification of patients for whom COX-2 inhibition therapy is indicated by allowing one to balance the patient's cardiovascular risk with the cancer's responsiveness to celecoxib...
  18. pmc Msh2 acts in medium-spiny striatal neurons as an enhancer of CAG instability and mutant huntingtin phenotypes in Huntington's disease knock-in mice
    Marina Kovalenko
    Center for Human Genetic Research, Massachusetts General Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 7:e44273. 2012
    ....
  19. pmc Analysis of TBX1 variation in patients with psychotic and affective disorders
    Birgit H Funke
    Harvard Partners Center for Genetics and Genomics, Boston, MA 02139, USA
    Mol Med 13:407-14. 2007
    ..Based on these results we conclude that TBX1 variation does not make a strong contribution to the genetic etiology of nonsyndromic forms of psychiatric disorders commonly seen in patients with 22q11DS...
  20. pmc Role of the mismatch repair gene, Msh6, in suppressing genome instability and radiation-induced mutations
    Julio Barrera-Oro
    Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
    Mutat Res 642:74-9. 2008
    ..These findings indicate that MutS alpha reduces spontaneous and IR-induced mutation in a cell type-dependant manner...
  21. pmc In vivo wide-area cellular imaging by side-view endomicroscopy
    Pilhan Kim
    Wellman Center for Photomedicine, Department of Dermatology, Harvard Medical School and Massachusetts General Hospital, Boston, MA, USA
    Nat Methods 7:303-5. 2010
    ..We monitored cell infiltration, vascular changes and tumor progression during inflammation and tumorigenesis in colon over several months...
  22. doi Mutation detection using automated fluorescence-based sequencing
    Kate T Montgomery
    Harvard Medical School Partners Healthcare Center for Genetics and Genomics, Boston, Massachusetts, USA
    Curr Protoc Hum Genet . 2008
    ..A high-throughput protocol is given that has been used to search for mutations in over 200 different genes at the Harvard Medical School - Partners Center for Genetics and Genomics (HPCGG, http://www.hpcgg.org/)...
  23. doi Genetics and genomics in the practice of medicine
    Victoria A Joshi
    Harvard Medical School, Partners HealthCare Center for Genetics and Genomics, Boston, Massachusetts, USA
    Gastroenterology 134:1284-8. 2008
  24. ncbi Common and distinct genomic events in sporadic colorectal cancer and diverse cancer types
    Eric S Martin
    Department of Medical Oncology, Belfer Institute for Innovative Cancer Science, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 67:10736-43. 2007
    ....
  25. ncbi Onset of abnormal blood and lymphatic vessel function and interstitial hypertension in early stages of carcinogenesis
    Jeroen Hagendoorn
    Edwin L Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital, 100 Blossom Street, Boston, MA 02114, USA
    Cancer Res 66:3360-4. 2006
    ..In addition, the lymphatic vessels in hyperplastic/dysplastic lesions were partly compressed and nonfunctional. These novel insights may aid early detection and treatment strategies for cancer...
  26. ncbi Response to treatment and survival of patients with non-small cell lung cancer undergoing somatic EGFR mutation testing
    Lecia V Sequist
    Massachusetts General Hospital Cancer Center, Harvard Medical School Partners Healthcare Center for Genetics and Genomics, MGH Department of Pathology, Dana Farber Cancer Institute, Boston, Massachusetts 02114, USA
    Oncologist 12:90-8. 2007
    ..6 years in mutation-negative patients, after adjusting for age, gender, and stage at diagnosis. Integrating molecular profiling into clinical care is feasible in NSCLC patients and provides useful clinical information...
  27. ncbi Epidermal growth factor receptor mutation testing in the care of lung cancer patients
    Lecia V Sequist
    Cancer Center and Department of Medicine, Massachusetts General Hospital, and Laboratory for Molecular Medicine, Harvard Medical School, Boston 02114, USA
    Clin Cancer Res 12:4403s-4408s. 2006
    ..It is likely that mutation testing and other molecular analyses will be most useful in these two clinical situations...
  28. ncbi A novel, single nucleotide polymorphism-based assay to detect 22q11 deletions
    Birgit H Funke
    Harvard Medical School Partners Healthcare Center for Genetics and Genomics, Cambridge, MA 02139, USA
    Genet Test 11:91-100. 2007
    ..Our SNP based assay is a highly accurate, sensitive, and specific method for the diagnosis of 22q11 deletion syndrome...
  29. ncbi Pharmacogenomics of lung cancer: with a view to address EGFR-targeted therapies
    Victoria A Joshi
    Harvard Medical School Partners Healthcare Center for Genetics and Genomics, Department of Pathology, Massachusetts General Hospital, MA, USA
    Pharmacogenomics 8:1211-20. 2007
    ..These principles are generally applicable to the field of targeted therapy and predictive pharmacogenomics...
  30. pmc Landscape of somatic retrotransposition in human cancers
    Eunjung Lee
    Center for Biomedical Informatics, Harvard Medical School, Boston, MA 02115, USA
    Science 337:967-71. 2012
    ....
  31. pmc Utilization of a whole genome SNP panel for efficient genetic mapping in the mouse
    Jennifer L Moran
    Genetics Division, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genome Res 16:436-40. 2006
    ..By using this approach, we identified DNA sequence changes in two ethylnitrosourea-induced mutants...
  32. pmc Analyzing Somatic Genome Rearrangements in Human Cancers by Using Whole-Exome Sequencing
    Lixing Yang
    Department of Biomedical Informatics, Harvard Medical School, Boston, MA 02115, USA
    Am J Hum Genet 98:843-56. 2016
    ....
  33. pmc An EGFR targeted PET imaging probe for the detection of colonic adenocarcinomas in the setting of colitis
    N Selcan Turker
    1 Athinoula A Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA
    Theranostics 4:893-903. 2014
    ..The imaging agent can detect colonic tumors with a high TBR for detection of in situ lesions in the setting of colitis, and opens the possibility for a new approach for screening high-risk patients. ..
  34. pmc Next-generation sequencing identifies rare variants associated with Noonan syndrome
    Peng Chieh Chen
    Department of Genetics, Harvard Medical School, Boston, MA 02115 Department of Medicine, Division of Genetics, Brigham and Women s Hospital, Boston, MA 02115 Institute of Clinical Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70457, Taiwan
    Proc Natl Acad Sci U S A 111:11473-8. 2014
    ..Taken together, our data suggest that next-generation sequencing can provide a useful adjunct to RASopathy diagnosis and emphasize that the standard clinical categories for RASopathies might not be adequate to describe all patients. ..
  35. pmc Genetic contributions to changes of fiber tracts of ventral visual stream in 22q11.2 deletion syndrome
    Zora Kikinis
    Psychiatry Neuroimaging Laboratory, Department of Psychiatry, Brigham and Women s Hospital, Harvard Medical School, 1249 Boylston Street, Boston, MA, 02115, USA
    Brain Imaging Behav 7:316-25. 2013
    ..We conclude that reduced axonal changes may be key to understanding the underlying pathology of WM leading to the visuo-spatial phenotype in 22q11.2DS. ..
  36. pmc The Creating an Optimal Warfarin Nomogram (CROWN) Study
    Todd S Perlstein
    Division of Cardiovascular Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Thromb Haemost 107:59-68. 2012
    ..07), and the time to achieve stable therapeutic anticoagulation (p < 0.001). In conclusion, warfarin pharmacogenetic dosing can be optimised in real time utilising observed PK/PD information in an adaptive fashion...
  37. pmc Copy number variation detection in whole-genome sequencing data using the Bayesian information criterion
    Ruibin Xi
    Center for Biomedical Informatics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:E1128-36. 2011
    ..We propose this statistical approach as a principled yet practical and efficient method to estimate CNVs in whole-genome sequencing data...
  38. pmc Haploinsufficiency of Flap endonuclease (Fen1) leads to rapid tumor progression
    Melanie Kucherlapati
    Department of Medicine and Harvard Partners Center for Genetics and Genomics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 99:9924-9. 2002
    ..The tumors from these mice show microsatellite instability. Because one copy of the Fen1 gene remained intact in tumors, Fen1 haploinsufficiency appears to lead to rapid progression of cancer...
  39. ncbi Increased susceptibility to UV-induced skin carcinogenesis in polymerase eta-deficient mice
    Qingcong Lin
    Harvard Medical School Partners Healthcare Center for Genetics and Genomics, Boston, MA 02115, USA
    Cancer Res 66:87-94. 2006
    ..5% of pol eta heterozygous mice also developed skin cancer during 5 months after a 5-month exposure to UV light, suggesting that humans who are heterozygous for mutations in pol eta may also have an increased risk of skin cancer...
  40. pmc APC-dependent suppression of colon carcinogenesis by PPARgamma
    Geoffrey D Girnun
    Dana Farber Cancer Institute and Department of Cell Biology, Harvard Medical School, One Jimmy Fund Way, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 99:13771-6. 2002
    ..This finding suggests a potentially important use for PPARgamma ligands as chemopreventative agents in colon cancer...
  41. pmc Rb and N-ras function together to control differentiation in the mouse
    Chiaki Takahashi
    Department of Medical Oncology and Medicine, Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
    Mol Cell Biol 23:5256-68. 2003
    ..Our findings suggest that the control of differentiation and proliferation by Rb are genetically separable...
  42. ncbi SOS1 mutations are rare in human malignancies: implications for Noonan Syndrome patients
    Kenneth D Swanson
    Cancer Biology Program, Division of Hematology Oncology, Department of Medicine, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Massachusetts, USA
    Genes Chromosomes Cancer 47:253-9. 2008
    ..Our findings suggest that SOS1 is not a significant human oncogene in most cancers. Furthermore, NS patients with SOS1 mutations may not be at increased risk of developing cancer...
  43. pmc Cathepsin B expression and survival in colon cancer: implications for molecular detection of neoplasia
    Andrew T Chan
    Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Cancer Epidemiol Biomarkers Prev 19:2777-85. 2010
    ..Proteases play a critical role in tumorigenesis and are upregulated in colorectal cancer and neoplastic polyps. In animal models, cathepsin B (CTSB)-activatable imaging agents show high enzyme activity within intestinal tumors...
  44. pmc Shared genetic causes of cardiac hypertrophy in children and adults
    Hiroyuki Morita
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    N Engl J Med 358:1899-908. 2008
    ..Despite morphologic similarities to genetic cardiomyopathies of adulthood, the contribution of genetics to childhood-onset hypertrophy is unknown...
  45. pmc Clinical significance of CTNNB1 mutation and Wnt pathway activation in endometrioid endometrial carcinoma
    Yuexin Liu
    Department of Pathology YL, LP, RRB, WZ and Department of Systems Biology GBM and Departments of Gynecologic Oncology and Reproductive Medicine and Cancer Biology KHL, AKS, The University of Texas MD Anderson Cancer Center, Houston, TX The Genome Institute, Washington University, St Louis, MO LD, ERM Department of Genetics, Harvard Medical School, Boston, MA RK Gynecology Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York City, NY DAL The Institute for Systems Biology, Seattle, WA IS
    J Natl Cancer Inst 106:. 2014
    ..Endometrioid endometrial carcinoma (EEC) is the most common form of endometrial carcinoma. The heterogeneous clinical course of EEC is an obstacle to individualized patient care...
  46. pmc Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial
    Daniel G Lee
    Department of Molecular Biology, Massachusetts General Hospital, Cambridge Street, Boston, Massachusetts 02114, USA
    Genome Biol 7:R90. 2006
    ..We therefore sequenced a highly virulent strain of P. aeruginosa, PA14, and compared it with a previously sequenced (and less pathogenic) strain, PAO1, to identify novel virulence genes...
  47. ncbi Mlh1 mediates tissue-specific regulation of mitotic recombination
    Changshun Shao
    Department of Genetics, Rutgers University, 604 Allison Road, Piscataway, NJ 08854 8082, USA
    Oncogene 23:9017-24. 2004
    ..Thus, different cell types respond differently to MLH1 deficiency, and the contribution of MR to tumorigenesis may be tissue-dependent in the absence of mismatch repair...
  48. pmc Association of the DTNBP1 locus with schizophrenia in a U.S. population
    Birgit Funke
    Harvard Medical School Partners Center for Genetics and Genomics, Boston, MA 02115, USA
    Am J Hum Genet 75:891-8. 2004
    ..005). Our study provides further evidence for a role of the DTNBP1 gene in the genetic etiology of schizophrenia...
  49. ncbi Mass spectrometry-based study of the plasma proteome in a mouse intestinal tumor model
    Kenneth E Hung
    Gastrointestinal Unit, Massachusetts General Hospital, 55 Fruit Street, Boston, 02114, USA
    J Proteome Res 5:1866-78. 2006
    ..These studies suggest that mass spectrometry-based approaches to examine the plasma proteome may prove to be a valuable method for determining the presence of intestinal tumors...