Pinfen Yang

Summary

Affiliation: Marquette University
Country: USA

Publications

  1. pmc Flagellar radial spoke protein 2 is a calmodulin binding protein required for motility in Chlamydomonas reinhardtii
    Pinfen Yang
    Department of Biology, Marquette University, Milwaukee, Wisconsin 53233, USA
    Eukaryot Cell 3:72-81. 2004
  2. pmc Novel LC8 mutations have disparate effects on the assembly and stability of flagellar complexes
    Pinfen Yang
    Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin 53233, USA
    J Biol Chem 284:31412-21. 2009
  3. pmc Radial spoke proteins of Chlamydomonas flagella
    Pinfen Yang
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA
    J Cell Sci 119:1165-74. 2006
  4. pmc Dimeric novel HSP40 is incorporated into the radial spoke complex during the assembly process in flagella
    Chun Yang
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA
    Mol Biol Cell 16:637-48. 2005
  5. pmc Dimeric heat shock protein 40 binds radial spokes for generating coupled power strokes and recovery strokes of 9 + 2 flagella
    Chun Yang
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA
    J Cell Biol 180:403-15. 2008
  6. pmc The flagellar motility of Chlamydomonas pf25 mutant lacking an AKAP-binding protein is overtly sensitive to medium conditions
    Chun Yang
    Department of Biological Sciences, Marquette University, Milwaukee WI 53233, USA
    Mol Biol Cell 17:227-38. 2006
  7. pmc Chlamydomonas mutants display reversible deficiencies in flagellar beating and axonemal assembly
    Mei Wei
    Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin, USA
    Cytoskeleton (Hoboken) 67:71-80. 2010
  8. pmc The versatile molecular complex component LC8 promotes several distinct steps of flagellar assembly
    Anjali Gupta
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53201, USA
    J Cell Biol 198:115-26. 2012
  9. pmc A flagellar A-kinase anchoring protein with two amphipathic helices forms a structural scaffold in the radial spoke complex
    Priyanka Sivadas
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53201, USA
    J Cell Biol 199:639-51. 2012
  10. doi request reprint Molecular tools for studying the radial spoke
    Xiaoyan Zhu
    Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin, USA
    Methods Enzymol 524:19-36. 2013

Research Grants

Collaborators

  • Yi Liu
  • Anjali Gupta
  • Kenneth W Foster
  • Maureen Wirschell
  • David R Mitchell
  • Ritsu Kamiya
  • Elizabeth F Smith
  • G J Pazour
  • J L Rosenbaum
  • S M King
  • George Witman
  • A Hallmann
  • Martin Lohr
  • CAROL DIECKMANN
  • Wallace Marshall
  • Arthur R Grossman
  • Xiaoyan Zhu
  • Chun Yang
  • Priyanka Sivadas
  • Mei Wei
  • Radhika Gopal
  • Heather A Owen
  • Pooja Kelekar
  • Sabeeha S Merchant
  • Triscia W Hendrickson
  • William D Meek
  • Jennifer M Dienes
  • Martin St Maurice
  • Steven Ball
  • Joanne C Long
  • Vadim N Gladyshev
  • Sathish Rajamani
  • Katerina Bisova
  • Junmin Pan
  • Marek Elias
  • Laurens Mets
  • Laurence Maréchal-Drouard
  • Leila Hornick
  • Heriberto Cerutti
  • Marc Hanikenne
  • Wirulda Pootakham
  • Simon E Prochnik
  • Chong Jian Chen
  • Patrick Ferris
  • Carolyn Napoli
  • Susan M Lucas
  • Karla Gendler
  • Harris Shapiro
  • Bernd Mueller-Roeber
  • Heidi Ledford
  • Krishna Niyogi
  • Stephane D Lemaire
  • Emilio Fernandez
  • Diego Mauricio Riaño-Pachón
  • Ming Kalanon
  • Vladimir V Kapitonov
  • David Gonzalez-Ballester
  • Sergey V Novoselov
  • Sara Lana Zimmer
  • Gregory Werner
  • Andrea Manuell
  • Martin T Croft
  • Robert Willows
  • Diego Martinez
  • David R Nelson
  • Astrid Terry
  • Peter Brokstein
  • Liang Hu Qu
  • Nedra Wilson
  • David Goodstein
  • Rachel Dent
  • James V Moroney
  • Charles Hauser
  • David Stern
  • Inna Dubchak
  • Richard T Sayre
  • Aaron Porter
  • Pierre Cardol
  • Jinal Jhaveri
  • Pamela Green
  • Valerie Cognat
  • Maria Mittag
  • Aimee M Terauchi
  • Telsa Mittelmeier
  • M Dudley Page
  • Lillian K Fritz-Laylin
  • Erika Lindquist
  • Wayne Riekhof
  • Chun Long Chen
  • Janneke Balk

Detail Information

Publications17

  1. pmc Flagellar radial spoke protein 2 is a calmodulin binding protein required for motility in Chlamydomonas reinhardtii
    Pinfen Yang
    Department of Biology, Marquette University, Milwaukee, Wisconsin 53233, USA
    Eukaryot Cell 3:72-81. 2004
    ..As predicted from the sequence, recombinant RSP2 binds calmodulin in a calcium-dependent manner. We postulate that RSP2 is a regulatory subunit of the radial spoke involved in localization of calmodulin for control of motility...
  2. pmc Novel LC8 mutations have disparate effects on the assembly and stability of flagellar complexes
    Pinfen Yang
    Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin 53233, USA
    J Biol Chem 284:31412-21. 2009
    ....
  3. pmc Radial spoke proteins of Chlamydomonas flagella
    Pinfen Yang
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA
    J Cell Sci 119:1165-74. 2006
    ..The human genes encoding these proteins are candidates for causing primary ciliary dyskinesia, a severe inherited disease involving missing or defective axonemal structures, including the radial spokes...
  4. pmc Dimeric novel HSP40 is incorporated into the radial spoke complex during the assembly process in flagella
    Chun Yang
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA
    Mol Biol Cell 16:637-48. 2005
    ....
  5. pmc Dimeric heat shock protein 40 binds radial spokes for generating coupled power strokes and recovery strokes of 9 + 2 flagella
    Chun Yang
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53233, USA
    J Cell Biol 180:403-15. 2008
    ..This enables 9 + 2 cilia and flagella to bend and switch to generate alternate power strokes and recovery strokes...
  6. pmc The flagellar motility of Chlamydomonas pf25 mutant lacking an AKAP-binding protein is overtly sensitive to medium conditions
    Chun Yang
    Department of Biological Sciences, Marquette University, Milwaukee WI 53233, USA
    Mol Biol Cell 17:227-38. 2006
    ..We postulate that radial spokes use the RIIa/AKAP module to regulate ciliary and flagellar beating; absence of the spoke RIIa protein exposes a medium-sensitive regulatory mechanism that is not obvious in wild-type Chlamydomonas...
  7. pmc Chlamydomonas mutants display reversible deficiencies in flagellar beating and axonemal assembly
    Mei Wei
    Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin, USA
    Cytoskeleton (Hoboken) 67:71-80. 2010
    ..Consistently, several organisms only possess one RSP4/6 gene. Gene duplication in Chlamydomonas enhances RS assembly to maintain optimal motility in various environments...
  8. pmc The versatile molecular complex component LC8 promotes several distinct steps of flagellar assembly
    Anjali Gupta
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53201, USA
    J Cell Biol 198:115-26. 2012
    ..These multiple effects shed new light on fundamental questions about LC8-containing complexes and axoneme assembly...
  9. pmc A flagellar A-kinase anchoring protein with two amphipathic helices forms a structural scaffold in the radial spoke complex
    Priyanka Sivadas
    Department of Biological Sciences, Marquette University, Milwaukee, WI 53201, USA
    J Cell Biol 199:639-51. 2012
    ..Thus, AHs and D/D domains constitute a versatile yet potentially promiscuous system for localizing various effector mechanisms. These results greatly expand the current concept about anchoring mechanisms and AKAPs...
  10. doi request reprint Molecular tools for studying the radial spoke
    Xiaoyan Zhu
    Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin, USA
    Methods Enzymol 524:19-36. 2013
    ..They could be adapted for the study of many other axonemal complexes and for classroom settings as well...
  11. pmc The DPY-30 domain and its flanking sequence mediate the assembly and modulation of flagellar radial spoke complexes
    Radhika Gopal
    Department of Biological Sciences, Marquette University, Milwaukee, WI, USA
    Mol Cell Biol 32:4012-24. 2012
    ..Thus, in general, D/D domains may function to localize molecular modules for both the assembly and modulation of macromolecular complexes...
  12. doi request reprint Isolation and analysis of radial spoke proteins
    Pooja Kelekar
    Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin, USA
    Methods Cell Biol 92:181-96. 2009
    ..With appropriate modifications, the lessons learned from the radial spoke complex may be applicable to other axonemal complexes...
  13. doi request reprint Radial Spokes-A Snapshot of the Motility Regulation, Assembly, and Evolution of Cilia and Flagella
    Xiaoyan Zhu
    The Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin 53201
    Cold Spring Harb Perspect Biol . 2016
    ..Recent studies have made remarkable progress on the radial spoke. This review will trace how the new structural, compositional, and evolutional insights pose significant implications on flagella biology and, conversely, ciliopathy...
  14. pmc The radial spokes and central apparatus: mechano-chemical transducers that regulate flagellar motility
    Elizabeth F Smith
    Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    Cell Motil Cytoskeleton 57:8-17. 2004
  15. ncbi request reprint Building a radial spoke: flagellar radial spoke protein 3 (RSP3) is a dimer
    Maureen Wirschell
    Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cell Motil Cytoskeleton 65:238-48. 2008
    ....
  16. pmc IC138 is a WD-repeat dynein intermediate chain required for light chain assembly and regulation of flagellar bending
    Triscia W Hendrickson
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mol Biol Cell 15:5431-42. 2004
    ....
  17. pmc The Chlamydomonas genome reveals the evolution of key animal and plant functions
    Sabeeha S Merchant
    Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Science 318:245-50. 2007
    ....

Research Grants1

  1. REGULATION OF DYNEIN-DRIVEN FLAGELLAR MOTILITY
    Pinfen Yang; Fiscal Year: 2009
    ..The results will also have broad impact on how dynein-driven motility is controlled and how kinases and calcium sensors are anchored in the microtubule cytoskeleton. ..