Saraswati Sukumar

Summary

Affiliation: Johns Hopkins University
Country: USA

Publications

  1. pmc Gene Methylation and Cytological Atypia in Random Fine-Needle Aspirates for Assessment of Breast Cancer Risk
    Vered Stearns
    Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland
    Cancer Prev Res (Phila) 9:673-82. 2016
  2. pmc Combined Treatment with Epigenetic, Differentiating, and Chemotherapeutic Agents Cooperatively Targets Tumor-Initiating Cells in Triple-Negative Breast Cancer
    Vanessa F Merino
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland
    Cancer Res 76:2013-24. 2016
  3. pmc Big punches come in nanosizes for chemoprevention
    Dipali Sharma
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21231 and Dipali Sharma
    Cancer Prev Res (Phila) 6:1007-10. 2013
  4. ncbi request reprint HOXA5 acts directly downstream of retinoic acid receptor beta and contributes to retinoic acid-induced apoptosis and growth inhibition
    Hexin Chen
    The Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231 1000, USA
    Cancer Res 67:8007-13. 2007
  5. pmc HOXB13 mediates tamoxifen resistance and invasiveness in human breast cancer by suppressing ERα and inducing IL-6 expression
    Nilay Shah
    Departments of Oncology, Surgery, and Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231 1000, USA
    Cancer Res 73:5449-58. 2013
  6. ncbi request reprint Identification of transcriptional targets of HOXA5
    Hexin Chen
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins Hospital, Baltimore, Maryland 21231 1000, USA
    J Biol Chem 280:19373-80. 2005
  7. pmc Epigenetic inactivation of the potential tumor suppressor gene FOXF1 in breast cancer
    Pang Kuo Lo
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cancer Res 70:6047-58. 2010
  8. ncbi request reprint Overexpression of glycosylphosphatidylinositol (GPI) transamidase subunits phosphatidylinositol glycan class T and/or GPI anchor attachment 1 induces tumorigenesis and contributes to invasion in human breast cancer
    Guojun Wu
    Department of Otolaryngology Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cancer Res 66:9829-36. 2006
  9. ncbi request reprint HOXB7, a homeodomain protein, is overexpressed in breast cancer and confers epithelial-mesenchymal transition
    Xinyan Wu
    Breast Cancer Program, Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cancer Res 66:9527-34. 2006
  10. ncbi request reprint Epigenetic suppression of secreted frizzled related protein 1 (SFRP1) expression in human breast cancer
    Pang Kuo Lo
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cancer Biol Ther 5:281-6. 2006

Detail Information

Publications87

  1. pmc Gene Methylation and Cytological Atypia in Random Fine-Needle Aspirates for Assessment of Breast Cancer Risk
    Vered Stearns
    Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland
    Cancer Prev Res (Phila) 9:673-82. 2016
    ..Thus, CMI is an excellent candidate breast cancer risk biomarker, warranting larger prospective studies to establish its utility for cancer risk assessment. Cancer Prev Res; 9(8); 673-82. ©2016 AACR. ..
  2. pmc Combined Treatment with Epigenetic, Differentiating, and Chemotherapeutic Agents Cooperatively Targets Tumor-Initiating Cells in Triple-Negative Breast Cancer
    Vanessa F Merino
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland
    Cancer Res 76:2013-24. 2016
    ..Collectively, our findings strongly suggest that entinostat potentiates doxorubicin-mediated cytotoxicity and retinoid-driven differentiation to achieve significant tumor regression in TNBC. Cancer Res; 76(7); 2013-24. ©2016 AACR...
  3. pmc Big punches come in nanosizes for chemoprevention
    Dipali Sharma
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21231 and Dipali Sharma
    Cancer Prev Res (Phila) 6:1007-10. 2013
    ..It will constitute the first successful multipronged attack at key pathways known to initiate and promote carcinogenesis...
  4. ncbi request reprint HOXA5 acts directly downstream of retinoic acid receptor beta and contributes to retinoic acid-induced apoptosis and growth inhibition
    Hexin Chen
    The Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231 1000, USA
    Cancer Res 67:8007-13. 2007
    ..These results strongly suggest that HOXA5 acts directly downstream of RARbeta and may contribute to retinoid-induced anticancer and chemopreventive effects...
  5. pmc HOXB13 mediates tamoxifen resistance and invasiveness in human breast cancer by suppressing ERα and inducing IL-6 expression
    Nilay Shah
    Departments of Oncology, Surgery, and Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231 1000, USA
    Cancer Res 73:5449-58. 2013
    ..Taken together, our results establish a function for HOXB13 in TAM resistance through direct suppression of ERα and they identify the IL-6 pathways as mediator of disease progression and recurrence...
  6. ncbi request reprint Identification of transcriptional targets of HOXA5
    Hexin Chen
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins Hospital, Baltimore, Maryland 21231 1000, USA
    J Biol Chem 280:19373-80. 2005
    ..Further functional analysis of these targets will allow us to better understand the diverse functions of HOXA5 in embryonic development and tumorigenesis...
  7. pmc Epigenetic inactivation of the potential tumor suppressor gene FOXF1 in breast cancer
    Pang Kuo Lo
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cancer Res 70:6047-58. 2010
    ..Therefore, our studies have identified FOXF1 as a potential tumor suppressor gene that is epigenetically silenced in breast cancer, which plays an essential role in regulating cell cycle progression to maintain genomic stability...
  8. ncbi request reprint Overexpression of glycosylphosphatidylinositol (GPI) transamidase subunits phosphatidylinositol glycan class T and/or GPI anchor attachment 1 induces tumorigenesis and contributes to invasion in human breast cancer
    Guojun Wu
    Department of Otolaryngology Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cancer Res 66:9829-36. 2006
    ..This aberrant growth is mediated, at least partially, by phosphorylation of paxillin, contributing to invasion and progression of breast cancer...
  9. ncbi request reprint HOXB7, a homeodomain protein, is overexpressed in breast cancer and confers epithelial-mesenchymal transition
    Xinyan Wu
    Breast Cancer Program, Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cancer Res 66:9527-34. 2006
    ..Thus, HOXB7 overexpression caused EMT in epithelial cells, accompanied by acquisition of aggressive properties of tumorigenicity, migration, and invasion...
  10. ncbi request reprint Epigenetic suppression of secreted frizzled related protein 1 (SFRP1) expression in human breast cancer
    Pang Kuo Lo
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cancer Biol Ther 5:281-6. 2006
    ..Based on the frequency of tumor-specific hypermethylation in this gene, SFRP1 could provide a valuable marker for breast cancer...
  11. pmc Genome-wide methylation analysis identifies genes specific to breast cancer hormone receptor status and risk of recurrence
    Mary Jo Fackler
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cancer Res 71:6195-207. 2011
    ..Furthermore, it defines candidate ER-specific markers and identifies potential markers predictive of outcome within ER subgroups...
  12. ncbi request reprint A role for the HOXB7 homeodomain protein in DNA repair
    Ethel Rubin
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Cancer Res 67:1527-35. 2007
    ..This is the first mechanistic study providing definitive evidence for the involvement of any HOX protein in DNA double-strand break repair...
  13. pmc Polyamine analogues down-regulate estrogen receptor alpha expression in human breast cancer cells
    Yi Huang
    Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    J Biol Chem 281:19055-63. 2006
    ..Taken together, these results suggest a novel antiestrogenic mechanism for specific polyamine analogues in human breast cancer cells...
  14. pmc HOXB7 Is an ERα Cofactor in the Activation of HER2 and Multiple ER Target Genes Leading to Endocrine Resistance
    Kideok Jin
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland
    Cancer Discov 5:944-59. 2015
    ..Repressing MYC using small-molecule inhibitors reverses these events and causes regression of breast cancer xenografts. The MYC-HOXB7-HER2 signaling pathway is eminently targetable in endocrine-resistant breast cancer...
  15. ncbi request reprint Quantitative promoter hypermethylation profiles of ductal carcinoma in situ in North American and Korean women: Potential applications for diagnosis
    Ji Shin Lee
    Breast Cancer Program, Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Cancer Biol Ther 7:1398-406. 2008
    ..For both North American and Korean women, QM-MSP analysis of a key panel of genes may be useful as an ancillary tool for DCIS detection in breast tissues...
  16. ncbi request reprint Two-color quantitative multiplex methylation-specific PCR
    Theresa Swift-Scanlan
    Johns Hopkins University School of Medicine, Baltimore, MD 21231 1000, USA
    Biotechniques 40:210-9. 2006
    ..Our modification decreases the cost and time of each real-time experiment by allowing increased throughput of clinical samples and by doubling either the number of genes or the number of samples that can be analyzed per real-time plate...
  17. pmc Intraductally administered pegylated liposomal doxorubicin reduces mammary stem cell function in the mammary gland but in the long term, induces malignant tumors
    Yong Soon Chun
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Breast Cancer Res Treat 135:201-8. 2012
    ..Unless there are fundamental species differences in PLD metabolism in rodents and humans, this finding seriously limits the consideration of i.duc PLD use in the clinic for treatment or prevention of breast cancer...
  18. pmc Preclinical and clinical evaluation of intraductally administered agents in early breast cancer
    Vered Stearns
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Sci Transl Med 3:106ra108. 2011
    ..If successful, administration of agents directly into the ductal system may allow for "breast-sparing mastectomy" in select women...
  19. ncbi request reprint A comparative study of Korean with Caucasian breast cancer reveals frequency of methylation in multiple genes correlates with breast cancer in young, ER, PR-negative breast cancer in Korean women
    Ji Shin Lee
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cancer Biol Ther 6:1114-20. 2007
    ....
  20. ncbi request reprint Quantitative multiplex methylation-specific PCR assay for the detection of promoter hypermethylation in multiple genes in breast cancer
    Mary Jo Fackler
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231 1000, USA
    Cancer Res 64:4442-52. 2004
    ..Such an approach could be used in a variety of small samples derived from different tissues, with these or different biomarkers to enhance detection of malignancy...
  21. pmc Somatic mutation and gain of copy number of PIK3CA in human breast cancer
    Guojun Wu
    Department of Otolaryngology Head and Neck Surgery, Head and Neck Cancer Research Division, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Breast Cancer Res 7:R609-16. 2005
    ..Even though PIK3CA amplification and somatic mutation have been reported previously in various kinds of human cancers, the genetic change in PIK3CA in human breast cancer has not been clearly identified...
  22. pmc Epithelial cell adhesion molecule (EpCAM) is overexpressed in breast cancer metastases
    Ashley Cimino
    Department of Pathology, Johns Hopkins Hospital, Weinberg 2242, 401 North Broadway, Baltimore, MD 21231, USA
    Breast Cancer Res Treat 123:701-8. 2010
    ..In conclusion, EpCAM is highly expressed in MBCs compared to matched PBCs, verifying that it is a promising therapeutic target...
  23. pmc MethySYBR, a novel quantitative PCR assay for the dual analysis of DNA methylation and CpG methylation density
    Pang Kuo Lo
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, 1650 Orleans St, CRBI 143, Baltimore, MD 21231, USA
    J Mol Diagn 11:400-14. 2009
    ..Therefore, the MethySYBR assay is a simple, highly specific, highly sensitive, high-throughput, and cost-effective method that is widely applicable to basic and clinical studies of DNA methylation...
  24. pmc The Notch pathway inhibits TGFβ signaling in breast cancer through HEYL-mediated crosstalk
    Liangfeng Han
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland
    Cancer Res 74:6509-18. 2014
    ..Therefore, repression of TGFβ signaling by Notch acting through HEYL may promote initiation of breast cancer...
  25. doi request reprint Hypermethylated genes as biomarkers of cancer in women with pathologic nipple discharge
    Mary Jo Fackler
    Departments of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Clin Cancer Res 15:3802-11. 2009
    ....
  26. pmc Intraductal administration of a polymeric nanoparticle formulation of curcumin (NanoCurc) significantly attenuates incidence of mammary tumors in a rodent chemical carcinogenesis model: Implications for breast cancer chemoprevention in at-risk populations
    Yong Soon Chun
    Departments of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21231 1000, USA
    Carcinogenesis 33:2242-9. 2012
    ..Our studies confirm the potential for i.duc NanoCurc as an alternative to the oral route for breast cancer chemoprevention in high-risk cohorts...
  27. pmc Functional activation of the estrogen receptor-α and aromatase by the HDAC inhibitor entinostat sensitizes ER-negative tumors to letrozole
    Gauri J Sabnis
    Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine and University of Maryland Greenebaum Cancer Center, Baltimore, Maryland 21201, USA
    Cancer Res 71:1893-903. 2011
    ..More generally, they provide a strong rationale for immediate clinical evaluation of combinations of histone deacetylase and aromatase inhibitors to treat ER-negative and endocrine-resistant breast cancers...
  28. pmc DNA methylation-related vitamin D receptor insensitivity in breast cancer
    Radharani Marik
    Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Cancer Biol Ther 10:44-53. 2010
    ..These data suggest that pharmacological reversal of VDR methylation may re-establish breast cancer cell susceptibility to differentiation therapy using Calcitriol...
  29. pmc Genetic and phenotypic diversity in breast tumor metastases
    Vanessa Almendro
    Authors Affiliations Departments of Medical Oncology and Biostatistics and Computational Biology, Dana Farber Cancer Institute Department of Medicine, Brigham and Women s Hospital Department of Medicine, Harvard Medical School Department of Biostatistics, Harvard School of Public Health, Boston Departments of Physics and Biology, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology Harvard Stem Cell Institute, Cambridge, Massachusetts Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, New York Departments of Pathology and Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland Department of Medical Oncology, Hospital Clinic, Institut d Investigacions Biomediques August Pi i Sunyer, Barcelona, Spain Department of Pathology, MizMedi Hospital, Seoul, South Korea Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel and Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, Utrecht, The Netherlands
    Cancer Res 74:1338-48. 2014
    ....
  30. pmc Epigenomics and breast cancer
    Pang Kuo Lo
    Johns Hopkins University School of Medicine, 1650 Orleans Street, CRBI 143, Baltimore, MD 21231, USA
    Pharmacogenomics 9:1879-902. 2008
    ..Furthermore, we highlight the significance of epigenetic alterations as predictive biomarkers and as new targets of anticancer therapy...
  31. pmc Targeting Glutamine Metabolism in Breast Cancer with Aminooxyacetate
    Preethi Korangath
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland
    Clin Cancer Res 21:3263-73. 2015
    ..Glutamine addiction in c-MYC-overexpressing breast cancer is targeted by the aminotransferase inhibitor, aminooxyacetate (AOA). However, the mechanism of ensuing cell death remains unresolved...
  32. pmc Biomarker modulation following short-term vorinostat in women with newly diagnosed primary breast cancer
    Vered Stearns
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Clin Cancer Res 19:4008-16. 2013
    ..We evaluated biomarker modulation in breast cancer tissues obtained from women with newly diagnosed invasive disease who received vorinostat and those who did not...
  33. pmc HOXC10 Expression Supports the Development of Chemotherapy Resistance by Fine Tuning DNA Repair in Breast Cancer Cells
    Helen Sadik
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland
    Cancer Res 76:4443-56. 2016
    ..Blocking HOXC10 function, therefore, presents a promising new strategy to overcome chemotherapy resistance in breast cancer. Cancer Res; 76(15); 4443-56. ©2016 AACR. ..
  34. ncbi request reprint Monitoring of Serum DNA Methylation as an Early Independent Marker of Response and Survival in Metastatic Breast Cancer: TBCRC 005 Prospective Biomarker Study
    Kala Visvanathan
    Kala Visvanathan, Johns Hopkins University School of Medicine and Bloomberg School of Public Health MaryJo S Fackler, Zhe Zhang, Zoila A Lopez Bujanda, Stacie C Jeter, Lori J Sokoll, Leslie M Cope, Christopher B Umbricht, David M Euhus, Saraswati Sukumar, and Antonio C Wolff, Johns Hopkins University School of Medicine, Baltimore, MD Elizabeth Garrett Mayer, Medical University of South Carolina, Charleston, SC Andres Forero, University of Alabama at Birmingham, Birmingham, AL Anna M Storniolo, Indiana University, Bloomington, IN Rita Nanda, University of Chicago, Chicago, IL Nancy U Lin, Dana Farber Cancer Institute, Boston, MA Lisa A Carey, University of North Carolina, Chapel Hill, NC and James N Ingle, Mayo Clinic, Rochester, MN
    J Clin Oncol . 2016
    ..004). Conclusion Methylation of this gene panel is a strong predictor of survival outcomes in MBC and may have clinical usefulness in risk stratification and disease monitoring...
  35. pmc HMGA1: a master regulator of tumor progression in triple-negative breast cancer cells
    Sandeep N Shah
    Hematology Division, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 8:e63419. 2013
    ..Future studies are needed to determine how to target HMGA1 in therapy...
  36. pmc Heterogeneity of breast cancer metastases: comparison of therapeutic target expression and promoter methylation between primary tumors and their multifocal metastases
    Julie M Wu
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Clin Cancer Res 14:1938-46. 2008
    ..A comprehensive comparison of biomarker expression between patients' primary breast carcinoma (PBC) and their metastatic breast carcinomas (MBC) has not been done...
  37. ncbi request reprint Identification of biomarkers for breast cancer in nipple aspiration and ductal lavage fluid
    Jinong Li
    Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA
    Clin Cancer Res 11:8312-20. 2005
    ..To establish a comprehensive proteomic approach for biomarker discovery and validation in breast fluid...
  38. ncbi request reprint Very high frequency of hypermethylated genes in breast cancer metastasis to the bone, brain, and lung
    Jyoti Mehrotra
    Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Clin Cancer Res 10:3104-9. 2004
    ....
  39. pmc BRCA1: linking HOX to breast cancer suppression
    Kideok Jin
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at Johns, Hopkins, Johns Hopkins University School of Medicine, 1650 Orleans Street, Room 143 CRB1, Baltimore, MD 21231, USA
    Breast Cancer Res 12:306. 2010
    ..This finding raises our hope that more, rather elusive targets of HOX genes important in tumor progression or suppression will be found in the future...
  40. pmc Effective treatment of ductal carcinoma in situ with a HER-2- targeted alpha-particle emitting radionuclide in a preclinical model of human breast cancer
    Takahiro Yoshida
    Department of Oncology, Johns Hopkins University School of Medicine, Maryland, USA
    Oncotarget 7:33306-15. 2016
    ..Our findings suggest that i.duc radioimmunotherapy using 225Ac-trastuzumab deserves greater attention for future clinical development as a treatment modality for early breast cancer. ..
  41. pmc ADP ribosylation by PARP-1 suppresses HOXB7 transcriptional activity
    Xinyan Wu
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 7:e40644. 2012
    ..More importantly, this study reveals a novel mechanism whereby polyADP-ribosylation regulates transcriptional activities of HOX proteins such as HOXB7 and HOXA7...
  42. ncbi request reprint Alterations in vascular gene expression in invasive breast carcinoma
    Belinda S Parker
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cancer Res 64:7857-66. 2004
    ..Together, our results provide unique insights into vascular regulation in breast tumors and suggest specific roles for genes in driving tumor angiogenesis...
  43. pmc Role of p53/p21(Waf1/Cip1) in the regulation of polyamine analogue-induced growth inhibition and cell death in human breast cancer cells
    Yi Huang
    The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cancer Biol Ther 4:1006-13. 2005
    ..Understanding the mechanism of p53-mediated cellular responses to polyamine analogue may help to improve the therapeutic efficacy of polyamine analogues in human breast cancer...
  44. ncbi request reprint Of Snail, mice, and women
    Nancy E Davidson
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at John Hopkins, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cancer Cell 8:173-4. 2005
    ..The identification of a molecular pathway involved in mammary tumor recurrence in a mouse model offers both opportunity and challenge to confirm, extend, and exploit these findings in the clinic...
  45. pmc Store-independent activation of Orai1 by SPCA2 in mammary tumors
    Mingye Feng
    Department of Physiology, School of Medicine, The Johns Hopkins University, Baltimore, MD 21205, USA
    Cell 143:84-98. 2010
    ..Our findings reveal a signaling pathway in which the Orai1-SPCA2 complex elicits constitutive store-independent Ca(2+) signaling that promotes tumorigenesis...
  46. pmc PIK3CA somatic mutations in breast cancer: Mechanistic insights from Langevin dynamics simulations
    Parminder K Mankoo
    Department of Biomedical Engineering and Institute for Computational Medicine, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Proteins 75:499-508. 2009
    ..Mutant-specific differences in the catalytic cleft and activation loop behavior suggest that structure-based mutant-specific inhibitors can be designed for PIK3CA-positive breast cancers...
  47. ncbi request reprint A PET rat model for assessing the effectiveness of new chemotherapies
    Paul T Winnard
    Department of Radiology and Radiological Sciences, The JohnsHopkins University School of Medicine, Baltimore, MD 21205, USA
    Cancer Biol Ther 7:538-9. 2008
  48. ncbi request reprint The genomic landscapes of human breast and colorectal cancers
    Laura D Wood
    Ludwig Center for Cancer Genetics and Therapeutics and Howard Hughes Medical Institute at Johns Hopkins Kimmel Cancer Center, Baltimore, MD 21231, USA
    Science 318:1108-13. 2007
    ..These results have implications for understanding the nature and heterogeneity of human cancers and for using personal genomics for tumor diagnosis and therapy...
  49. pmc The HOXB7 protein renders breast cancer cells resistant to tamoxifen through activation of the EGFR pathway
    Kideok Jin
    Breast Cancer Program, Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231 1000, USA
    Proc Natl Acad Sci U S A 109:2736-41. 2012
    ..These studies suggest that HOXB7 acts as a key regulator, orchestrating a major group of target molecules in the oncogenic hierarchy. Functional antagonism of HOXB7 could circumvent tamoxifen resistance...
  50. pmc The dual role of FOXF2 in regulation of DNA replication and the epithelial-mesenchymal transition in breast cancer progression
    Pang Kuo Lo
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA Electronic address
    Cell Signal 28:1502-19. 2016
    ..These findings suggest that FOXF2 has a dual role in breast tumorigenesis and functions as either a tumor suppressor or an oncogene depending on the breast tumor subtype. ..
  51. pmc Collagen I fiber density increases in lymph node positive breast cancers: pilot study
    Samata M Kakkad
    The Johns Hopkins University School of Medicine, The Russell H Morgan Department of Radiology and Radiological Science, Baltimore, Maryland, USA
    J Biomed Opt 17:116017. 2012
    ..High Col1 fiber density in primary breast tumors is associated with breast cancer metastasis and may serve as an imaging biomarker of metastasis...
  52. pmc Phosphoproteomic Analysis Identifies Focal Adhesion Kinase 2 (FAK2) as a Potential Therapeutic Target for Tamoxifen Resistance in Breast Cancer
    Xinyan Wu
    From the McKusick Nathans Institute of Genetic Medicine and Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
    Mol Cell Proteomics 14:2887-900. 2015
    ..Our studies suggest that FAK2 is a potential therapeutic target for the management of hormone-refractory breast cancers. ..
  53. ncbi request reprint Tumor-specific changes in mtDNA content in human cancer
    Elizabeth Mambo
    Department of Otolaryngology Head and Neck Surgery, Head and Neck Cancer Research Division, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Int J Cancer 116:920-4. 2005
    ..Our findings suggest that mtDNA content can be used as a molecular diagnostic tool to help identify genetic abnormalities in human tumors...
  54. ncbi request reprint Ductal access for prevention and therapy of mammary tumors
    Satoshi Murata
    Department of Oncology, Johns Hopkins University School of Medicine, 1650 Orleans Street, Baltimore, MD 21231, USA
    Cancer Res 66:638-45. 2006
    ..These studies suggest that this approach has potential for application to prevention and neoadjuvant therapy of early breast cancer...
  55. pmc Do breast cancer cell lines provide a relevant model of the patient tumor methylome?
    Leslie M Cope
    Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 9:e105545. 2014
    ....
  56. pmc A Self-Folding Hydrogel In Vitro Model for Ductal Carcinoma
    Hye Rin Kwag
    1 Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland
    Tissue Eng Part C Methods 22:398-407. 2016
    ..We believe that self-folding processes and associated tubular, curved, and folded constructs like the ones demonstrated here can facilitate the design of more accurate in vitro models for investigating ductal carcinoma. ..
  57. doi request reprint The Hox genes and their roles in oncogenesis
    Nilay Shah
    Nilay Shah and Saraswati Sukumar are at the Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Nat Rev Cancer 10:361-71. 2010
    ..Therefore, Hox gene expression could be important in diagnosis and therapy...
  58. ncbi request reprint Benzoylphenylurea sulfur analogues with potent antitumor activity
    Gurulingappa Hallur
    Division of Chemical Therapeutics, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231, USA
    J Med Chem 49:2357-60. 2006
    ..6n was more effective than 1 in causing apoptosis of MCF-7 cells. When compared to other drugs with a similar mechanism of action, 6n retained significant ability to inhibit tubulin assembly, with an IC(50) of 2.1 microM...
  59. pmc Clostridium perfringens enterotoxin elicits rapid and specific cytolysis of breast carcinoma cells mediated through tight junction proteins claudin 3 and 4
    Scott L Kominsky
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Am J Pathol 164:1627-33. 2004
    ..Thus, expression of CLDN 3 and 4 sensitizes primary breast carcinomas to CPE-mediated cytolysis and emphasizes the potential of CPE in breast cancer therapy...
  60. ncbi request reprint Intraductal therapy for the prevention of breast cancer
    Lisa Jacobs
    Johns Hopkins University, 600 North Wolfe Street, Osler 624, Baltimore, MD 21287, USA
    Curr Opin Investig Drugs 11:646-52. 2010
    ..As a result of these advances, intraductal treatment in high-risk populations is being investigated in clinical trials...
  61. pmc Combining the strength of genomics, nanoparticle technology, and direct intraductal delivery for breast cancer treatment
    Wei Wen Teo
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 1650, Orleans Street, CRB1 143, Baltimore, MD, 21231, USA
    Breast Cancer Res 16:306. 2014
    ..This study strengthens the concept of targeting overexpressed genes by using small interfering RNA and bypassing systemic immunity through local intraductal delivery. ..
  62. ncbi request reprint DNA methylation regulates MicroRNA expression
    Liangfeng Han
    Predoctoral Training Program in Human Genetics, McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cancer Biol Ther 6:1284-8. 2007
    ..We also found that HOXA3 and HOXD10 were putative targets of mir-10a, one of the differentially expressed miRNAs that is located in HOX gene cluster...
  63. pmc Molecular pathways: current role and future directions of the retinoic acid pathway in cancer prevention and treatment
    Roisin M Connolly
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA
    Clin Cancer Res 19:1651-9. 2013
    ..Robust evaluation of RARβ and downstream genes may permit optimized use of retinoids in the solid tumor arena...
  64. ncbi request reprint Clostridium perfringens enterotoxin as a novel-targeted therapeutic for brain metastasis
    Scott L Kominsky
    Department of Orthopedic Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cancer Res 67:7977-82. 2007
    ..These data suggest that CPE therapy may have efficacy against a wide variety of brain metastases without CNS toxicity...
  65. pmc Hoxb7 inhibits transgenic HER-2/neu-induced mouse mammary tumor onset but promotes progression and lung metastasis
    Hexin Chen
    The Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland, USA
    Cancer Res 68:3637-44. 2008
    ..Our data show, for the first time, that deregulated expression of Hoxb7 in mammary tumor cells can significantly modulate HER-2/neu-oncogene induced tumorigenesis in vivo...
  66. pmc Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis
    Esak Lee
    1 Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA 2 Department of Chemical and Biomolecular Engineering, School of Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Nat Commun 5:4715. 2014
    ..This study demonstrates anti-metastatic activities of multiple repurposed drugs, blocking a self-reinforcing paracrine loop between breast cancer cells and LECs. ..
  67. pmc HOX genes: Major actors in resistance to selective endocrine response modifiers
    Kideok Jin
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD, United States Department of Biomedical Engineering at Johns Hopkins, 720 Rutland Avenue, 617 Traylor Bldg, Baltimore, MD 21205, United States Electronic address
    Biochim Biophys Acta 1865:105-10. 2016
    ..We also discuss, in particular, the contributions of several members of the HOX gene family to endocrine resistant breast cancer. ..
  68. ncbi request reprint Quantitative multiplex methylation-specific PCR analysis doubles detection of tumor cells in breast ductal fluid
    Mary Jo Fackler
    Department of Oncology, Johns Hopkins University School of Medicine, Baltimore Maryland, USA
    Clin Cancer Res 12:3306-10. 2006
    ..We hypothesized that quantitative analysis of methylated genes will provide enhanced detection of cancer cells present in cytologic specimens...
  69. ncbi request reprint Coupling the transcriptional regulatory action of Brn-3b to the cell cycle clock
    Tao Zhu
    Breast Cancer Program, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Cancer Biol Ther 3:324-5. 2004
  70. pmc HOXA5-induced apoptosis in breast cancer cells is mediated by caspases 2 and 8
    Hexin Chen
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, 1650 Orleans Street, CRB 410, Baltimore, MD 21231 1000, USA
    Mol Cell Biol 24:924-35. 2004
    ..These results indicate that expression of HOXA5 can induce apoptosis through an apoptotic mechanism mediated by caspases 2 and 8...
  71. ncbi request reprint Methylation profiling of benign and malignant breast lesions and its application to cytopathology
    Robert T Pu
    Department of Pathology, Division of Cytopathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA
    Mod Pathol 16:1095-101. 2003
    ..We then analyzed 17 breast FNA biopsy samples with an indeterminate diagnosis. In this setting, MSP had a high specificity (100%) and modest sensitivity (67%) for identifying malignancy...
  72. ncbi request reprint DNA methylation of RASSF1A, HIN-1, RAR-beta, Cyclin D2 and Twist in in situ and invasive lobular breast carcinoma
    Mary Jo Fackler
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, 401 N Broadway, Baltimore, MD 21231 2410, USA
    Int J Cancer 107:970-5. 2003
    ..01). These results suggest that these 2 types of tumors share many common methylation patterns and some molecular differences. Additional studies might lend further understanding into the etiology and clinical behavior of this tumor type...
  73. ncbi request reprint Novel agents for chemoprevention, screening methods, and sampling issues
    Mary Jo Fackler
    Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 21231 1000, USA
    J Mammary Gland Biol Neoplasia 8:75-89. 2003
    ..Factors that could contribute to a meaningful choice of the chemopreventive agents and the design of clinical trials are discussed...
  74. ncbi request reprint Significant allelic loss of ANX7region (10q21) in hormone receptor negative breast carcinomas
    Ximena Leighton
    Department of Anatomy, Physiology and Genetics, and Institute for Molecular Medicine, Uniformed Services University School of Medicine USUHS, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Cancer Lett 210:239-44. 2004
    ..06). These results suggest that the ANX7 gene, or other genes of the 10q21 region, could be functionally related to breast cancer, probably influencing the hormone receptor expression associated with poor prognosis during development...
  75. ncbi request reprint Mitoxantrone mediates demethylation and reexpression of cyclin d2, estrogen receptor and 14.3.3sigma in breast cancer cells
    Belinda S Parker
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland 20231, USA
    Cancer Biol Ther 2:259-63. 2003
    ..The effect of mitoxantrone on re-expression of key genes involved in cell cycle regulation, and ensuing death of the cells may be an additional, previously undiscovered mechanism of action of mitoxantrone...
  76. ncbi request reprint Mutational hotspot in exon 20 of PIK3CA in breast cancer among Singapore Chinese
    Xiaohui Liang
    Oncology Research Institute, National University of Singapore, Singapore
    Cancer Biol Ther 5:544-8. 2006
    ..The results suggest that mutation of PIK3CA might contribute to development of early stage breast cancer and could provide a potent target for early diagnosis and therapy...
  77. ncbi request reprint Loss of the tight junction protein claudin-7 correlates with histological grade in both ductal carcinoma in situ and invasive ductal carcinoma of the breast
    Scott L Kominsky
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Oncogene 22:2021-33. 2003
    ..In summary, these studies provide insight into the potential role of CLDN-7 in the progression and ability of breast cancer cells to disseminate...
  78. ncbi request reprint Epigenetic biomarkers and breast cancer: cause for optimism
    Kala Visvanathan
    Clin Cancer Res 12:6591-3. 2006
  79. ncbi request reprint Distant metastasis in breast cancer: molecular mechanisms and therapeutic targets
    Belinda Parker
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
    Cancer Biol Ther 2:14-21. 2003
    ..These advances will enable the development of therapies targeted towards blocking the outgrowth of metastatic cells...
  80. ncbi request reprint Hypermethylation in histologically distinct classes of breast cancer
    Young Kyung Bae
    Department of Pathology, Yeungnam University College of Medicine, Daegu, South Korea
    Clin Cancer Res 10:5998-6005. 2004
    ..We sought to determine whether the extent of hypermethylation or defined profiles of gene hypermethylation are associated with biological characteristics of breast cancers...
  81. ncbi request reprint HOX genes: emerging stars in cancer
    Hexin Chen
    Breast Cancer Program, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland USA
    Cancer Biol Ther 2:524-5. 2003
  82. ncbi request reprint Role of homeobox genes in normal mammary gland development and breast tumorigenesis
    Hexin Chen
    Breast Cancer Program, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland, USA
    J Mammary Gland Biol Neoplasia 8:159-75. 2003
    ..Recent studies of homeobox genes in breast cancer cells and primary tumors indicate that they may also play a contributory or causal role in tumorigenesis by regulating the cell cycle, apoptosis, angiogenesis, and/or metastasis...
  83. ncbi request reprint Estrogen receptor/progesterone receptor-negative breast cancers of young African-American women have a higher frequency of methylation of multiple genes than those of Caucasian women
    Jyoti Mehrotra
    Breast Cancer Program, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland, USA
    Clin Cancer Res 10:2052-7. 2004
    ....
  84. ncbi request reprint ETS genes in breast cancer: a step in the right direction
    Xinyan Wu
    Johns Hopkins University School of Medicine Baltimore, Maryland 21231, USA
    Cancer Biol Ther 6:83-4. 2007
  85. ncbi request reprint The role of WT1 in oncogenesis: tumor suppressor or oncogene?
    David M Loeb
    Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland, USA
    Int J Hematol 76:117-26. 2002
    ..The prognostic implications of altered WT1 expression and the potential for immunotherapy aimed at WT1 are also discussed...
  86. ncbi request reprint RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer
    Quek Choon Lau
    Oncology Research Institute and Department of Pathology, National University of Singapore, 10 Medical Drive, Singapore 117597
    Cancer Res 66:6512-20. 2006
    ..Frequent protein mislocalization and methylation could render RUNX3 a valuable marker for early detection and risk assessment...
  87. ncbi request reprint Molecular definition of breast tumor heterogeneity
    Michail Shipitsin
    Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cancer Cell 11:259-73. 2007
    ..Our data suggest prognostic relevance of CD44+ cells and therapeutic targeting of distinct tumor cell populations...