Genomes and Genes
Affiliation: Johns Hopkins University
- Lysosomes: Regulators of autophagy in the retinal pigmented epitheliumDebasish Sinha
Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA Electronic address
Exp Eye Res 144:46-53. 2016..This suggests that βA3/A1-crystallin may also be a potential target for therapeutic intervention in AMD. In this review, we focus on effector molecules that impact the lysosomal-autophagic pathway in RPE cells. ..
- βA3/A1-Crystallin controls anoikis-mediated cell death in astrocytes by modulating PI3K/AKT/mTOR and ERK survival pathways through the PKD/Bit1-signaling axisB Ma
The Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
Cell Death Dis 2:e217. 2011..We propose that βA3/A1-crystallin is a novel regulator of both life and death decisions in ocular astrocytes...
- βA3/A1-crystallin is required for proper astrocyte template formation and vascular remodeling in the retinaDebasish Sinha
The Wilmer Eye Institute, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore, MD, 21231, USA
Transgenic Res 21:1033-42. 2012....
- betaA3/A1-crystallin in astroglial cells regulates retinal vascular remodeling during developmentDebasish Sinha
Department of Ophthalmology, The Johns Hopkins University School of Medicine, Bunting Blaustein Cancer Research Building II, 1550 Orleans St, Room 146, Baltimore, MD 21231, USA
Mol Cell Neurosci 37:85-95. 2008..Our data implicate betaA3/A1-crystallin as an important regulatory factor mediating vascular patterning and remodeling in the retina...
- A spontaneous mutation affects programmed cell death during development of the rat eyeDebasish Sinha
Department of Ophthalmology, School of Medicine, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21287, USA
Exp Eye Res 80:323-35. 2005..The transient layer of Chievitz persists in adult Nuc1 retina, indicative of delayed development. Hence, Nuc1 is a novel mutation that could be an eye-specific regulator of apoptosis...
- Impaired endolysosomal function disrupts Notch signalling in optic nerve astrocytesMallika Valapala
The Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
Nat Commun 4:1629. 2013..We postulate that βA3/A1-crystallin is essential for normal endolysosomal acidification, and thereby, normal activation of Notch signalling in astrocytes...
- A potential role for beta- and gamma-crystallins in the vascular remodeling of the eyeCheng Zhang
Department of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, MD 21287, USA
Dev Dyn 234:36-47. 2005..Interestingly, in human PFV (persistent fetal vasculature) disease, where the hyaloid vasculature abnormally persists after birth, we show that astrocytes express both VEGF and crystallins...
- βA3/A1-crystallin is a critical mediator of STAT3 signaling in optic nerve astrocytesMallika Valapala
Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
Sci Rep 5:8755. 2015..Consistent with this idea, Cryba1 knockout mice exhibit an abnormal astrocyte pattern and defective remodeling of retinal vessels. ..
- The effect of nerve growth factor on corneal sensitivity after laser in situ keratomileusisMyung Jin Joo
Wilmer Eye Institute, The Johns Hopkins Hospital, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA
Arch Ophthalmol 122:1338-41. 2004..To determine if topically administered nerve growth factor (NGF) plays a role in accelerating the recovery of corneal sensitivity after laser in situ keratomileusis (LASIK)...
- A developmental defect in astrocytes inhibits programmed regression of the hyaloid vasculature in the mammalian eyeCheng Zhang
The Wilmer Eye Institute, The Johns Hopkins University School of Medicine, 400 North Broadway, Baltimore, MD 21231, USA
Eur J Cell Biol 90:440-8. 2011..This abnormal association of astrocytes with the hyaloid artery may impede the normal macrophage-mediated remodeling and regression of the hyaloid system...
- Mutation in the βA3/A1-crystallin gene impairs phagosome degradation in the retinal pigmented epithelium of the ratJ Samuel Zigler
The Wilmer Eye Institute, The Johns Hopkins University School of Medicine, 600 N Wolfe Street, Baltimore, MD 21287, USA
J Cell Sci 124:523-31. 2011..A3/A1-crystallin is a novel lysosomal protein in RPE, essential for degradation of phagocytosed material...
- VEGF 165 b in the developing vasculatures of the fetal human eyeTakayuki Baba
Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
Dev Dyn 241:595-607. 2012..The localization of VEGF(165) b was distinctly different from VEGF(165) both spatially and temporally and it was often associated with nucleus in progenitors...
- Modulation of V-ATPase by βA3/A1-Crystallin in Retinal Pigment Epithelial CellsMallika Valapala
Department of Ophthalmology, Johns Hopkins University School of Medicine, 21287, Baltimore, MD, USA
Adv Exp Med Biol 854:779-84. 2016..Taken together, these results suggest a novel mechanism by which βA3/A1-crystallin regulates lysosomal function by modulating the activity of V-ATPase. ..
- Increased Lipocalin-2 in the retinal pigment epithelium of Cryba1 cKO mice is associated with a chronic inflammatory responseMallika Valapala
Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Aging Cell 13:1091-4. 2014..LCN2 may contribute to induction of a chronic inflammatory response in this mouse model with AMD-like pathology. ..
- A novel rat model to study the functions of macrophages during normal development and pathophysiology of the eyeStacey Hose
Department of Ophthalmology, School of Medicine, The Johns Hopkins University, 600 North Wolfe Street Jefferson 3 127A, Baltimore, MD 21287, USA
Immunol Lett 96:299-302. 2005..Inflammation is known to have an enhanced influx of phagocytic cells. Our data suggests that subpopulations of macrophages perform distinct functions in inducing apoptosis and phagocytic activity during normal conditions and in disease...
- Expression of βA3/A1-crystallin in the developing and adult rat eyeGeetha Parthasarathy
Wilmer Eye Institute, The Johns Hopkins University School of Medicine, 400 N Broadway, Smith Research Building, M035, Baltimore, MD 21287, USA
J Mol Histol 42:59-69. 2011....
- βA3/A1-crystallin and persistent fetal vasculature (PFV) disease of the eyeJ Samuel Zigler
Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
Biochim Biophys Acta 1860:287-98. 2016..PFV causes serious congenital pathologies and is responsible for as much as 5% of blindness in the United States...
- A Spontaneous Missense Mutation in Branched Chain Keto Acid Dehydrogenase Kinase in the Rat Affects Both the Central and Peripheral Nervous SystemsJ Samuel Zigler
Department of Ophthalmology, The Johns Hopkins University School of Medicine, Baltimore, MD, United States of America
PLoS ONE 11:e0160447. 2016..The frogleg phenotype shares important characteristics with a previously described Bckdk knockout mouse and with human subjects with Bckdk mutations. In addition, we report novel data regarding peripheral neuropathy of the hind limbs. ..
- Activating the AKT2/NFκB/LCN-2 axis elicits an inflammatory response in age-related macular degenerationSayan Ghosh
Department of Ophthalmology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA
J Pathol . 2016..Our results demonstrate that both in our animal model and in human AMD the AKT2/NFκB/LCN-2 signalling axis is involved in activating the inflammatory response, making this pathway a potential target for AMD treatment...
- βA3/A1-crystallin: more than a lens proteinJ Samuel Zigler
The Johns Hopkins University School of Medicine, The Wilmer Eye Institute, 400 North Broadway, Smith Building Room M037, Baltimore, MD 21231, USA Electronic address
Prog Retin Eye Res 44:62-85. 2015..We provide evidence that the cellular function of βA3/A1-crystallin involves its interaction with V-ATPase, the proton pump responsible for acidification of the endolysosomal system. ..
- Expression pattern of an evolutionarily conserved splice variant in the rat Tacc2 geneBhaja K Padhi
Ophthalmology, Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland Hazard Identification Division, Environmental Health Science and Research Bureau, Health Canada, Ottawa, Ontario, Canada
Genesis 52:378-86. 2014....
- Comparative efficacy of topical gatifloxacin with ciprofloxacin, amikacin, and clarithromycin in the treatment of experimental Mycobacterium chelonae keratitisJoon Young Hyon
Ocular Microbiology and Immunology Laboratory, Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, MD 21287 9121, USA
Arch Ophthalmol 122:1166-9. 2004..To determine the comparative efficacy of topical gatifloxacin with ciprofloxacin, fortified amikacin, and clarithromycin against Mycobacterium chelonae keratitis in an animal model...
- Neuroprotection of photoreceptors by minocycline in light-induced retinal degenerationCheng Zhang
Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287 9238, USA
Invest Ophthalmol Vis Sci 45:2753-9. 2004..This study was conducted to evaluate the neuroprotective effect of minocycline and the role of microglia in light-induced retinal degeneration...
- Cannabinoid receptors in conjunctival epithelium: identification and functional propertiesMaría Iribarne
Facultad de Ciencias Biomedicas, Universidad Austral, Pilar, Buenos Aires, Argentina
Invest Ophthalmol Vis Sci 49:4535-44. 2008..The endocannabinoid system may be regulating these processes. Therefore, the authors explored the presence and properties of cannabinoid receptors (CB1 and CB2) in conjunctival epithelial cells...
- Downregulation of class II transactivator (CIITA) expression by synthetic cannabinoid CP55,940Celine Gongora
Institut de Biotechnologie et Pharmacologie, CNRS UMR 5094, Montpellier, Cedex 5, France
Immunol Lett 91:11-6. 2004..These data suggests that cannabinoids affect MHC class II expression through actions on CIITA at the transcriptional level...
- MODULATION OF CIITA BY CANNABINOIDS IN HUMAN MICROGLIADebasish Sinha; Fiscal Year: 2004..Microglia form the first line of defense during infection in the CNS and are involved in various immune diseases including AIDS. ..
- A CRYSTALLIN MUTATION WITH ABNORMAL ASTROCYTES AND RETINAL VESSELSDebasish Sinha; Fiscal Year: 2010..We believe that studies on the Nuc1 rat will provide new insights into the cellular and molecular mechanisms that regulate vascular development including the molecular interactions among neurons, astrocytes and endothelial cells. ..