Affiliation: Iowa State University
- Predicting success of oligomerized pool engineering (OPEN) for zinc finger target site sequencesJeffry D Sander
Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, MA 02129, USA
BMC Bioinformatics 11:543. 2010..Because generation of ZFPs using OPEN requires considerable effort, a computational method for identifying the sites in any given gene that are most likely to be successfully targeted by this method is desirable...
- ZFNGenome: a comprehensive resource for locating zinc finger nuclease target sites in model organismsDeepak Reyon
Department of Genetics, Iowa State University, Ames, IA 50011, USA
BMC Genomics 12:83. 2011....
- High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleasesFeng Zhang
Department of Genetics, Cell Biology, and Development and Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA
Proc Natl Acad Sci U S A 107:12028-33. 2010..Taken together, our results suggest that it should now be possible to obtain mutations in any Arabidopsis target gene regardless of its mutant phenotype...
- In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sitesJeffry D Sander
Molecular Pathology Unit, Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA 02129, USA, Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, MA 02129, USA, Department of Pathology, Harvard Medical School, Boston, MA 02115, USA, Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 02115, USA, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 01238, USA, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA and Howard Hughes Medical Institute, Chevy Chase, MD 02815, USA
Nucleic Acids Res 41:e181. 2013..Our improved method should enable more comprehensive profiling of ZFN specificities. ..