Eric Betzig

Summary

Affiliation: Howard Hughes Medical Institute
Country: USA

Publications

  1. doi request reprint High-speed, low-photodamage nonlinear imaging using passive pulse splitters
    Na Ji
    Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA
    Nat Methods 5:197-202. 2008
  2. ncbi request reprint Imaging intracellular fluorescent proteins at nanometer resolution
    Eric Betzig
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Science 313:1642-5. 2006
  3. pmc Real-time imaging of Huntingtin aggregates diverting target search and gene transcription
    Li Li
    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
    elife 5:. 2016
  4. pmc Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics
    Hari Shroff
    Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA
    Nat Methods 5:417-23. 2008
  5. pmc 3D imaging of Sox2 enhancer clusters in embryonic stem cells
    Zhe Liu
    Junior Fellow Program, Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
    elife 3:e04236. 2014
  6. pmc Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
    Thomas A Planchon
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
    Nat Methods 8:417-23. 2011
  7. pmc High-density three-dimensional localization microscopy across large volumes
    Wesley R Legant
    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
    Nat Methods 13:359-65. 2016
  8. doi request reprint Advances in the speed and resolution of light microscopy
    Na Ji
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
    Curr Opin Neurobiol 18:605-16. 2008
  9. doi request reprint Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues
    Na Ji
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
    Nat Methods 7:141-7. 2010
  10. pmc Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes
    Hari Shroff
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Proc Natl Acad Sci U S A 104:20308-13. 2007

Collaborators

Detail Information

Publications24

  1. doi request reprint High-speed, low-photodamage nonlinear imaging using passive pulse splitters
    Na Ji
    Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA
    Nat Methods 5:197-202. 2008
    ....
  2. ncbi request reprint Imaging intracellular fluorescent proteins at nanometer resolution
    Eric Betzig
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Science 313:1642-5. 2006
    ....
  3. pmc Real-time imaging of Huntingtin aggregates diverting target search and gene transcription
    Li Li
    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
    elife 5:. 2016
    ..Together, our results provide insights into how mHtt dynamically forms aggregates and disrupts the finely-balanced gene control mechanisms in neuronal cells. ..
  4. pmc Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics
    Hari Shroff
    Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA
    Nat Methods 5:417-23. 2008
    ..By allowing observation of a wide variety of nanoscale dynamics, live-cell PALM provides insights into molecular assembly during the initiation, maturation and dissolution of cellular processes...
  5. pmc 3D imaging of Sox2 enhancer clusters in embryonic stem cells
    Zhe Liu
    Junior Fellow Program, Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
    elife 3:e04236. 2014
    ..Our results suggest an integrated model linking cis-element 3D spatial distribution to local-versus-global target search modalities essential for regulating eukaryotic gene transcription. ..
  6. pmc Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
    Thomas A Planchon
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
    Nat Methods 8:417-23. 2011
    ..3 μm, speeds up to nearly 200 image planes per second and the ability to noninvasively acquire hundreds of 3D data volumes from single living cells encompassing tens of thousands of image frames...
  7. pmc High-density three-dimensional localization microscopy across large volumes
    Wesley R Legant
    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
    Nat Methods 13:359-65. 2016
    ..We also demonstrate super-resolution correlative imaging with protein-specific photoactivable fluorophores, providing a mutually compatible, single-platform alternative to correlative light-electron microscopy over large volumes. ..
  8. doi request reprint Advances in the speed and resolution of light microscopy
    Na Ji
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
    Curr Opin Neurobiol 18:605-16. 2008
    ....
  9. doi request reprint Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues
    Na Ji
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
    Nat Methods 7:141-7. 2010
    ..In particular, results from fixed mouse cortical slices illustrate our ability to improve signal and resolution to depths of 400 microm...
  10. pmc Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes
    Hari Shroff
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Proc Natl Acad Sci U S A 104:20308-13. 2007
    ..The simplicity, minimal invasiveness, resolution, and speed of the technique all suggest its potential to directly visualize molecular interactions within cellular structures at the nanometer scale...
  11. pmc Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution
    Bi Chang Chen
    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
    Science 346:1257998. 2014
    ..The results provide a visceral reminder of the beauty and the complexity of living systems. ..
  12. pmc A localized Wnt signal orients asymmetric stem cell division in vitro
    Shukry J Habib
    Department of Developmental Biology, Howard Hughes Medical Institute, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 265 Campus Drive, Stanford, CA 94305, USA
    Science 339:1445-8. 2013
    ..We suggest that a spatially restricted Wnt signal induces an oriented cell division that generates distinct cell fates at predictable positions relative to the Wnt source...
  13. pmc Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens
    Liang Gao
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
    Cell 151:1370-85. 2012
    ..elegans embryos. Bessel beam structured plane illumination thus promises new insights into complex biological phenomena that require 4D subcellular spatiotemporal detail in either a single or multicellular context...
  14. pmc Photoactivated localization microscopy (PALM) of adhesion complexes
    Hari Shroff
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA
    Curr Protoc Cell Biol . 2008
    ..A particular emphasis is placed on the instrumentation and photoactivatable fluorescent protein (PA-FP) tags necessary to achieve PALM images at approximately 20 nm resolution in 5 to 30 min in fixed cells...
  15. pmc ADVANCED IMAGING. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics
    Dong Li
    Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
    Science 349:aab3500. 2015
    ..In addition, we examined mitochondria, actin, and the Golgi apparatus dynamics in three dimensions. ..
  16. pmc Vinculin is required for cell polarization, migration, and extracellular matrix remodeling in 3D collagen
    Ingo Thievessen
    Laboratory of Cell and Tissue Morphodynamics, Cell Biology and Physiology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA Biophysics Group, Department of Physics, Friedrich Alexander University of Erlangen Nuremberg, Erlangen, Germany Physiology Course and Cellular Dynamics Program, Marine Biological Laboratory, Woods Hole, Massachusetts, USA Third Physics Institute Biophysics, Georg August University, Gottingen, Germany Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA California Life Company, South San Francisco, California, USA Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA Department of Chemistry, Stony Brook University, Stony Brook, New York, USA Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan Department of Biological Science, National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA
    FASEB J 29:4555-67. 2015
    ..Our results suggest that vinculin promotes directionally persistent cell migration and tension-dependent ECM remodeling in complex 3D environments by increasing cell-ECM adhesion and traction force generation...
  17. pmc Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue
    Kai Wang
    Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA
    Nat Commun 6:7276. 2015
    ..The method enables in vivo two-photon morphological and functional imaging down to 700 μm inside the mouse brain. ..
  18. doi request reprint Imaging live-cell dynamics and structure at the single-molecule level
    Zhe Liu
    Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA Electronic address
    Mol Cell 58:644-59. 2015
    ..This review discusses the concepts and strategies of structural and functional imaging in living cells at the single-molecule level with minimal perturbations to the specimen. ..
  19. pmc Characterization and adaptive optical correction of aberrations during in vivo imaging in the mouse cortex
    Na Ji
    Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA
    Proc Natl Acad Sci U S A 109:22-7. 2012
    ..The corrections allowed us to detect smaller neuronal structures at greater contrast and also improve the signal-to-noise ratio during functional Ca(2+) imaging in single neurons...
  20. doi request reprint Response to Comment on "Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics"
    Dong Li
    National Laboratory of Biological Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, P R China Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147
    Science 352:527. 2016
    ..Sahl et al in their Comment raise criticisms of our work that fall into three classes: image artifacts, resolution criteria, and comparative performance on live cells. We explore each of these in turn. ..
  21. pmc Rapid adaptive optical recovery of optimal resolution over large volumes
    Kai Wang
    Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA
    Nat Methods 11:625-8. 2014
    ..We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain. ..
  22. pmc Single-molecule dynamics of enhanceosome assembly in embryonic stem cells
    Jiji Chen
    Transcription Imaging Consortium, Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA
    Cell 156:1274-85. 2014
    ..Our findings also reveal fundamental aspects of gene and developmental regulation by fine-tuning TF dynamics and influence of the epigenome on target search parameters...
  23. pmc Subnuclear segregation of genes and core promoter factors in myogenesis
    Jie Yao
    Janelia Farm Research Campus, The Single Cell Biochemistry Consortium, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA
    Genes Dev 25:569-80. 2011
    ..We suggest that intranuclear sequestration of core transcription components and their target genes provides an additional mechanism for promoter selectivity during differentiation...
  24. doi request reprint High-density mapping of single-molecule trajectories with photoactivated localization microscopy
    Suliana Manley
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Methods 5:155-7. 2008
    ..By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes...