GARY B RUVKUN

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc The ERI-6/7 helicase acts at the first stage of an siRNA amplification pathway that targets recent gene duplications
    Sylvia E J Fischer
    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 7:e1002369. 2011
  2. pmc DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity
    Christian G Riedel
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nat Cell Biol 15:491-501. 2013
  3. pmc Identification of small RNA pathway genes using patterns of phylogenetic conservation and divergence
    Yuval Tabach
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nature 493:694-8. 2013
  4. pmc Induction of cytoprotective pathways is central to the extension of lifespan conferred by multiple longevity pathways
    David E Shore
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America
    PLoS Genet 8:e1002792. 2012
  5. pmc PIWI associated siRNAs and piRNAs specifically require the Caenorhabditis elegans HEN1 ortholog henn-1
    Taiowa A Montgomery
    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 8:e1002616. 2012
  6. pmc Repression of germline RNAi pathways in somatic cells by retinoblastoma pathway chromatin complexes
    Xiaoyun Wu
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America
    PLoS Genet 8:e1002542. 2012
  7. pmc Dual regulation of the lin-14 target mRNA by the lin-4 miRNA
    Zhen Shi
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America Biological Sciences in Dental Medicine Program, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 8:e75475. 2013
  8. pmc Functional genomic analysis of C. elegans molting
    Alison R Frand
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, USA
    PLoS Biol 3:e312. 2005
  9. doi request reprint The perfect storm of tiny RNAs
    Gary Ruvkun
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nat Med 14:1041-5. 2008
  10. ncbi request reprint The 20 years it took to recognize the importance of tiny RNAs
    Gary Ruvkun
    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Fruit Street, Boston, Massachusetts 02114, USA
    Cell 116:S93-6, 2 p following S96. 2004

Collaborators

Detail Information

Publications45

  1. pmc The ERI-6/7 helicase acts at the first stage of an siRNA amplification pathway that targets recent gene duplications
    Sylvia E J Fischer
    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 7:e1002369. 2011
    ..Thus, like several other siRNA-associated Argonautes with a conserved RNaseH motif, ERGO-1 appears to be required for siRNA maturation...
  2. pmc DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity
    Christian G Riedel
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nat Cell Biol 15:491-501. 2013
    ..Thus, we give insight into the mechanisms of DAF-16/FOXO-mediated transcriptional regulation and establish a critical link between ATP-dependent chromatin remodelling and lifespan regulation...
  3. pmc Identification of small RNA pathway genes using patterns of phylogenetic conservation and divergence
    Yuval Tabach
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nature 493:694-8. 2013
    ....
  4. pmc Induction of cytoprotective pathways is central to the extension of lifespan conferred by multiple longevity pathways
    David E Shore
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America
    PLoS Genet 8:e1002792. 2012
    ....
  5. pmc PIWI associated siRNAs and piRNAs specifically require the Caenorhabditis elegans HEN1 ortholog henn-1
    Taiowa A Montgomery
    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 8:e1002616. 2012
    ..Thus, PIWI-class Argonaute proteins are specifically adapted to associate with methylated small RNAs in C. elegans...
  6. pmc Repression of germline RNAi pathways in somatic cells by retinoblastoma pathway chromatin complexes
    Xiaoyun Wu
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America
    PLoS Genet 8:e1002542. 2012
    ..Regulation of small RNA pathway genes by human retinoblastoma may also underlie its role as a tumor suppressor gene...
  7. pmc Dual regulation of the lin-14 target mRNA by the lin-4 miRNA
    Zhen Shi
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America Biological Sciences in Dental Medicine Program, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 8:e75475. 2013
    ..Our results point to the importance of translational inhibition in silencing of lin-14 by the lin-4 miRNA. ..
  8. pmc Functional genomic analysis of C. elegans molting
    Alison R Frand
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, USA
    PLoS Biol 3:e312. 2005
    ..Many molting genes are conserved in parasitic nematodes responsible for human disease, and thus represent attractive targets for pesticide and pharmaceutical development...
  9. doi request reprint The perfect storm of tiny RNAs
    Gary Ruvkun
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nat Med 14:1041-5. 2008
  10. ncbi request reprint The 20 years it took to recognize the importance of tiny RNAs
    Gary Ruvkun
    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Fruit Street, Boston, Massachusetts 02114, USA
    Cell 116:S93-6, 2 p following S96. 2004
  11. pmc The microRNA miR-1 regulates a MEF-2-dependent retrograde signal at neuromuscular junctions
    David J Simon
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Cell 133:903-15. 2008
    ..We propose that miR-1 refines synaptic function by coupling changes in muscle activity to changes in presynaptic function...
  12. ncbi request reprint Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants
    Duo Wang
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 436:593-7. 2005
    ..These findings suggest that mutations in Rb pathway components cause cells to revert to patterns of gene expression normally restricted to germ cells. Rb may act by a similar mechanism to transform mammalian cells...
  13. pmc A whole-genome RNAi Screen for C. elegans miRNA pathway genes
    Devin H Parry
    Department of Genetics, Harvard Medical School, Richard B Simches Research Building, 185 Cambridge Street, CPZN 7250, Boston, Massachusetts 02114 2790, USA
    Curr Biol 17:2013-22. 2007
    ..An exploration of which protein cofactors are necessary for a miRNA to downregulate a target gene should reveal more fully the molecular mechanisms by which miRNAs are processed, trafficked, and regulate their target genes...
  14. ncbi request reprint Control of developmental timing by micrornas and their targets
    Amy E Pasquinelli
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Annu Rev Cell Dev Biol 18:495-513. 2002
    ..Instead of inventing new gene functions, even subtle changes in temporal expression of pre-existing control genes can result in speciation by altering the time at which they function...
  15. pmc Fat metabolism links germline stem cells and longevity in C. elegans
    Meng C Wang
    Department of Molecular Biology, Massachusetts General Hospital, and Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
    Science 322:957-60. 2008
    ..This lipase is a key factor in the lipid hydrolysis and increased longevity that are induced by decreased insulin signaling. These results suggest a link between C. elegans fat metabolism and longevity...
  16. ncbi request reprint Systematic interactome mapping and genetic perturbation analysis of a C. elegans TGF-beta signaling network
    Muneesh Tewari
    Center for Cancer Systems Biology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Mol Cell 13:469-82. 2004
    ..Integrating interactome maps with systematic genetic perturbations may be useful for developing a systems biology approach to this and other signaling modules...
  17. ncbi request reprint The mir-84 and let-7 paralogous microRNA genes of Caenorhabditis elegans direct the cessation of molting via the conserved nuclear hormone receptors NHR-23 and NHR-25
    Gabriel D Hayes
    Department of Genetics, Harvard Medical School and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Development 133:4631-41. 2006
    ..The synergistic action of miRNA paralogs in development may be a general feature of the diversified miRNA gene family...
  18. pmc The exonuclease ERI-1 has a conserved dual role in 5.8S rRNA processing and RNAi
    Harrison W Gabel
    Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, CPZN7250, 185 Cambridge Street, Boston, Massachusetts 02114, USA
    Nat Struct Mol Biol 15:531-3. 2008
    ..8S ribosomal RNA in both C. elegans and S. pombe. In C. elegans, two protein isoforms of ERI-1 are localized to the cytoplasm, and each has distinct functions in ribosomal RNA processing and negative regulation of RNA interference...
  19. pmc Lifespan regulation by evolutionarily conserved genes essential for viability
    Sean P Curran
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America
    PLoS Genet 3:e56. 2007
    ..These results suggest that insulin-signaling pathways play a role in regulation of aging at any stage in life...
  20. pmc A soma-to-germline transformation in long-lived Caenorhabditis elegans mutants
    Sean P Curran
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nature 459:1079-84. 2009
    ..These results indicate that the acquisition of germline characteristics by the somatic cells of C. elegans mutants with increased longevity contributes to their increased health and survival...
  21. ncbi request reprint A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans
    Scott Kennedy
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 427:645-9. 2004
    ..Thus, ERI-1 is a negative regulator that may normally function to limit the duration, cell-type specificity or endogenous functions of RNA interference...
  22. ncbi request reprint Computational and experimental identification of C. elegans microRNAs
    Yonatan Grad
    The Lipper Center for Computational Genetics and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 11:1253-63. 2003
    ..Based on hypotheses underlying our computational methods, we estimate that the C. elegans genome may encode between 140 and 300 miRNAs and potentially many more...
  23. ncbi request reprint Intercellular signaling of reproductive development by the C. elegans DAF-9 cytochrome P450
    Ho Yi Mak
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
    Development 131:1777-86. 2004
    ..Thus, daf-9 may integrate outputs from daf-2 and daf-7 signaling pathways to relay neuroendocrine signals through synthesis of a lipophilic hormone...
  24. ncbi request reprint The tiny RNA world
    GARY B RUVKUN
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, USA
    Harvey Lect 99:1-21. 2003
  25. ncbi request reprint Functional tests of enhancer conservation between distantly related species
    Ilya Ruvinsky
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Wellman 8, Boston, MA 02114, USA
    Development 130:5133-42. 2003
    ....
  26. pmc Rictor/TORC2 regulates fat metabolism, feeding, growth, and life span in Caenorhabditis elegans
    Alexander A Soukas
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Genes Dev 23:496-511. 2009
    ..These data indicate that Rictor/TORC2 is a nutrient-sensitive complex with outputs to AKT and SGK to modulate the assessment of food quality and signal to fat metabolism, growth, feeding behavior, reproduction, and life span...
  27. ncbi request reprint Long-lived C. elegans daf-2 mutants are resistant to bacterial pathogens
    Danielle A Garsin
    Department of Genetics, Massachusetts General Hospital, Boston, MA 02114, USA
    Science 300:1921. 2003
  28. ncbi request reprint A genetic interaction between the vesicular acetylcholine transporter VAChT/UNC-17 and synaptobrevin/SNB-1 in C. elegans
    Gisela M Sandoval
    Program in Neuroscience, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Neurosci 9:599-601. 2006
    ..These results suggest an association of vesicular soluble N-ethylmaleimide-sensitive-factor attachment protein receptor (SNARE) components with vesicular neurotransmitter transporters...
  29. pmc Two membrane-associated tyrosine phosphatase homologs potentiate C. elegans AKT-1/PKB signaling
    Patrick J Hu
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, USA
    PLoS Genet 2:e99. 2006
    ..elegans Akt/PKB signaling by cell autonomous and cell nonautonomous mechanisms. Similar molecules may modulate Akt/PKB signaling in human endocrine tissues...
  30. pmc daf-28 encodes a C. elegans insulin superfamily member that is regulated by environmental cues and acts in the DAF-2 signaling pathway
    Weiqing Li
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
    Genes Dev 17:844-58. 2003
    ..This DAF-28 mutant is likely to be poisonous to wild-type DAF-28 and other insulins...
  31. ncbi request reprint Insulin receptor substrate and p55 orthologous adaptor proteins function in the Caenorhabditis elegans daf-2/insulin-like signaling pathway
    Catherine A Wolkow
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    J Biol Chem 277:49591-7. 2002
    ..elegans mutants with defects in DAF-2 signaling. Interestingly, IST-1 appears to be required for signaling through a pathway that may act in parallel to AGE-1/PI3K...
  32. pmc Identification of many microRNAs that copurify with polyribosomes in mammalian neurons
    John Kim
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 101:360-5. 2004
    ..Moreover, all of the miRNAs that were tested cofractionate with polyribosomes, the sites of active translation. These findings indicate that a large, diverse population of miRNAs may function to regulate translation in mammalian neurons...
  33. pmc Gene activities that mediate increased life span of C. elegans insulin-like signaling mutants
    Andrew V Samuelson
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Genes Dev 21:2976-94. 2007
    ..The activities of these genes may normally decline during aging...
  34. ncbi request reprint DAF-16 target genes that control C. elegans life-span and metabolism
    Siu Sylvia Lee
    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, 50 Blossom Street, Boston, MA 02114, USA
    Science 300:644-7. 2003
    ..elegans, and RNA interference inactivation of the candidates showed that many of these genes mediate distinct aspects of daf-16 function, including longevity, metabolism, and development...
  35. pmc Trans-splicing in C. elegans generates the negative RNAi regulator ERI-6/7
    Sylvia E J Fischer
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nature 455:491-6. 2008
    ..The ERI-6/7 protein is a superfamily I helicase that both negatively regulates the exogenous RNAi pathway and functions in an endogenous RNAi pathway...
  36. ncbi request reprint MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression
    Jennifer H Mansfield
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Genet 36:1079-83. 2004
    ..Furthermore, miR-196a negatively regulates Hoxb8, indicating that its restricted expression pattern probably reflects a role in the patterning function of the Hox complex...
  37. ncbi request reprint Systematic analysis of genes required for synapse structure and function
    Derek Sieburth
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nature 436:510-7. 2005
    ..Twenty-four genes encoded proteins that were localized to presynaptic specializations. Loss-of-function mutations in 12 genes caused defects in presynaptic structure...
  38. ncbi request reprint Functional genomic analysis of RNA interference in C. elegans
    John K Kim
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Science 308:1164-7. 2005
    ..We demonstrate that some of these genes are also required for germline and somatic transgene silencing. Moreover, the physical interactions among these potential RNAi factors suggest links to other RNA-dependent gene regulatory pathways...
  39. ncbi request reprint Detection of broadly expressed neuronal genes in C. elegans
    Ilya Ruvinsky
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, 185 Cambridge Street, Simches 7, Boston, MA 02114, USA
    Dev Biol 302:617-26. 2007
    ..The known involvement of many of these genes in neurogenesis and physiology of the nervous system supports the utility of this set for future targeted analyses...
  40. ncbi request reprint Polygenic control of Caenorhabditis elegans fat storage
    Ho Yi Mak
    Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA
    Nat Genet 38:363-8. 2006
    ..This pathway also includes genes encoding intraflagellar transport proteins and cyclic nucleotide gated channels, demonstrating that C. elegans fat storage is under polygenic control...
  41. ncbi request reprint A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity
    Siu Sylvia Lee
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nat Genet 33:40-8. 2003
    ..These data suggest that the longer lifespan of C. elegans with compromised mitochrondria cannot simply be assigned to lower free radical production and suggest a more complex coupling of metabolism and longevity...
  42. ncbi request reprint Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes
    Kaveh Ashrafi
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nature 421:268-72. 2003
    ....
  43. doi request reprint The most conserved genome segments for life detection on Earth and other planets
    Thomas A Isenbarger
    Department of Molecular Biology, Massachusetts General Hospital, and Microbial Sciences Initiative, Harvard University, Cambridge, MA, USA
    Orig Life Evol Biosph 38:517-33. 2008
    ..This set of sequences defines a core set of DNA regions that have changed the least over billions of years of evolution and provides a means to identify and classify divergent life, including ancestrally related life on other planets...
  44. pmc C. elegans major fats are stored in vesicles distinct from lysosome-related organelles
    Eyleen J O'Rourke
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Cell Metab 10:430-5. 2009
    ..elegans, allowing for efficient and accurate genetic and functional genomic screens for genes that control fat accumulation at the organismal level...
  45. pmc Biosynthesis of the Caenorhabditis elegans dauer pheromone
    Rebecca A Butcher
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 106:1875-9. 2009
    ..Thus, daf-22 and dhs-28 are required for the biosynthesis of the short-chain fatty acid-derived side chains of the dauer pheromone and link dauer pheromone production to metabolic state...