G Ruvkun

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Caenorhabditis elegans Akt/PKB transduces insulin receptor-like signals from AGE-1 PI3 kinase to the DAF-16 transcription factor
    S Paradis
    Department of Molecular Biology, Massachusetts General Hospital MGH and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114 USA
    Genes Dev 12:2488-98. 1998
  2. pmc Regulation of DAF-2 receptor signaling by human insulin and ins-1, a member of the unusually large and diverse C. elegans insulin gene family
    S B Pierce
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Genes Dev 15:672-86. 2001
  3. ncbi request reprint The taxonomy of developmental control in Caenorhabditis elegans
    G Ruvkun
    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
    Science 282:2033-41. 1998
  4. ncbi request reprint Regulation of C. elegans DAF-16 and its human ortholog FKHRL1 by the daf-2 insulin-like signaling pathway
    R Y Lee
    Present addresses WormBase, Division of Biology, California Institute of Technology 156 29, Pasadena, CA 91125, USA
    Curr Biol 11:1950-7. 2001
  5. ncbi request reprint The Caenorhabditis elegans lim-6 LIM homeobox gene regulates neurite outgrowth and function of particular GABAergic neurons
    O Hobert
    Massachusetts General Hospital, Department of Molecular Biology, Harvard Medical School, Department of Genetics, Boston, MA 02114, USA
    Development 126:1547-62. 1999
  6. pmc Isoform-specific mutations in the Caenorhabditis elegans heterochronic gene lin-14 affect stage-specific patterning
    B J Reinhart
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Genetics 157:199-209. 2001
  7. ncbi request reprint Phosphatidylinositol 3-kinase signaling inhibits DAF-16 DNA binding and function via 14-3-3-dependent and 14-3-3-independent pathways
    C M Cahill
    Diabetes Unit, Massachusetts General Hospital and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114, USA
    J Biol Chem 276:13402-10. 2001
  8. ncbi request reprint The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
    B J Reinhart
    Department of Molecular Biology, Massachusetts General Hospital, and Harvard Medical School, Boston 02114, USA
    Nature 403:901-6. 2000
  9. ncbi request reprint Regulation of ectodermal and excretory function by the C. elegans POU homeobox gene ceh-6
    T R Burglin
    Department of Molecular Biology, Massachusetts General Hospital, Fruit Street, Boston, MA 02114, USA
    Development 128:779-90. 2001
  10. ncbi request reprint A bulged lin-4/lin-14 RNA duplex is sufficient for Caenorhabditis elegans lin-14 temporal gradient formation
    I Ha
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA
    Genes Dev 10:3041-50. 1996

Collaborators

Detail Information

Publications39

  1. pmc Caenorhabditis elegans Akt/PKB transduces insulin receptor-like signals from AGE-1 PI3 kinase to the DAF-16 transcription factor
    S Paradis
    Department of Molecular Biology, Massachusetts General Hospital MGH and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114 USA
    Genes Dev 12:2488-98. 1998
    ..This demonstrates that Akt/PKB activity is not necessarily dependent on AGE-1 PI3K activity. akt-1 and akt-2 are expressed in overlapping patterns in the nervous system and in tissues that are remodeled during dauer formation...
  2. pmc Regulation of DAF-2 receptor signaling by human insulin and ins-1, a member of the unusually large and diverse C. elegans insulin gene family
    S B Pierce
    Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Genes Dev 15:672-86. 2001
    ..Of five other ins genes tested, the only other one bearing a predicted C peptide also antagonizes daf-2 signaling, whereas four ins genes without a C peptide do not, indicating functional diversity within the ins family...
  3. ncbi request reprint The taxonomy of developmental control in Caenorhabditis elegans
    G Ruvkun
    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
    Science 282:2033-41. 1998
    ....
  4. ncbi request reprint Regulation of C. elegans DAF-16 and its human ortholog FKHRL1 by the daf-2 insulin-like signaling pathway
    R Y Lee
    Present addresses WormBase, Division of Biology, California Institute of Technology 156 29, Pasadena, CA 91125, USA
    Curr Biol 11:1950-7. 2001
    ..Together, these observations suggest that the combined effects of transcriptional and posttranslational regulation of daf-16 transduce insulin-like signals in C. elegans and perhaps more generally...
  5. ncbi request reprint The Caenorhabditis elegans lim-6 LIM homeobox gene regulates neurite outgrowth and function of particular GABAergic neurons
    O Hobert
    Massachusetts General Hospital, Department of Molecular Biology, Harvard Medical School, Department of Genetics, Boston, MA 02114, USA
    Development 126:1547-62. 1999
    ..elegans and suggest that LIM homeobox genes share the common theme of controlling terminal neural differentiation steps that when disrupted lead to specific neuroanatomical and neural function defects...
  6. pmc Isoform-specific mutations in the Caenorhabditis elegans heterochronic gene lin-14 affect stage-specific patterning
    B J Reinhart
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Genetics 157:199-209. 2001
    ....
  7. ncbi request reprint Phosphatidylinositol 3-kinase signaling inhibits DAF-16 DNA binding and function via 14-3-3-dependent and 14-3-3-independent pathways
    C M Cahill
    Diabetes Unit, Massachusetts General Hospital and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114, USA
    J Biol Chem 276:13402-10. 2001
    ..Thus, our results demonstrate at least two mechanisms, one 14-3-3-dependent and the other 14-3-3-independent, whereby PI 3-kinase signaling regulates DAF-16 DNA binding and transcription function...
  8. ncbi request reprint The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans
    B J Reinhart
    Department of Molecular Biology, Massachusetts General Hospital, and Harvard Medical School, Boston 02114, USA
    Nature 403:901-6. 2000
    ..We propose that the sequential stage-specific expression of the lin-4 and let-7 regulatory RNAs triggers transitions in the complement of heterochronic regulatory proteins to coordinate developmental timing...
  9. ncbi request reprint Regulation of ectodermal and excretory function by the C. elegans POU homeobox gene ceh-6
    T R Burglin
    Department of Molecular Biology, Massachusetts General Hospital, Fruit Street, Boston, MA 02114, USA
    Development 128:779-90. 2001
    ..We propose that one evolutionary conserved function of the POU-III transcription factor class could be the regulation of genes that mediate secretion/osmoregulation...
  10. ncbi request reprint A bulged lin-4/lin-14 RNA duplex is sufficient for Caenorhabditis elegans lin-14 temporal gradient formation
    I Ha
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA
    Genes Dev 10:3041-50. 1996
    ..This suggests that a specific secondary structure of lin-4/lin-14 RNA duplex that may be recognized by an accessory protein, rather than an RNA duplex per se, is required in vivo for the generation of the LIN-14 temporal gradient...
  11. ncbi request reprint Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans
    B Wightman
    Department of Molecular Biology, Massachusetts General Hospital, Boston 02114
    Cell 75:855-62. 1993
    ..These data suggest a molecular mechanism for Lin-14p temporal gradient formation: the lin-4 RNAs base pair to sites in the lin-14 3'UTR to form multiple RNA duplexes that down-regulate lin-14 translation...
  12. pmc A PDK1 homolog is necessary and sufficient to transduce AGE-1 PI3 kinase signals that regulate diapause in Caenorhabditis elegans
    S Paradis
    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Genes Dev 13:1438-52. 1999
    ..The activating pdk-1 mutation is located in a conserved region of the kinase domain; the equivalent amino acid substitution in human PDK1 activates its kinase activity toward mammalian Akt/PKB...
  13. pmc An insulin-like signaling pathway affects both longevity and reproduction in Caenorhabditis elegans
    H A Tissenbaum
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA
    Genetics 148:703-17. 1998
    ..The regulation of fertility, life span, and metabolism by an insulin-like signaling pathway is similar to the endocrine regulation of metabolism and fertility by mammalian insulin signaling...
  14. ncbi request reprint The lin-41 RBCC gene acts in the C. elegans heterochronic pathway between the let-7 regulatory RNA and the LIN-29 transcription factor
    F J Slack
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
    Mol Cell 5:659-69. 2000
    ..We suggest that late larval activation of let-7 RNA expression downregulates LIN-41 to relieve inhibition of lin-29...
  15. ncbi request reprint Dominant gain-of-function mutations that lead to misregulation of the C. elegans heterochronic gene lin-14, and the evolutionary implications of dominant mutations in pattern-formation genes
    G Ruvkun
    Department of Molecular Biology, Massachusetts General Hospital, Boston
    Dev Suppl 1:47-54. 1991
    ..The normal down-regulation of lin-14 within 10 h of hatching is not determined by the passage of time per se, but rather is triggered when feeding induces post-embryonic development.(ABSTRACT TRUNCATED AT 250 WORDS)..
  16. ncbi request reprint Molecular biology. Glimpses of a tiny RNA world
    G Ruvkun
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Science 294:797-9. 2001
  17. pmc A conserved LIM protein that affects muscular adherens junction integrity and mechanosensory function in Caenorhabditis elegans
    O Hobert
    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114
    J Cell Biol 144:45-57. 1999
    ..In addition to its localization to adherens junctions UNC-97 can also be detected in the nucleus, suggesting multiple functions for this LIM domain protein...
  18. ncbi request reprint Regulation of Caenorhabditis elegans RNA interference by the daf-2 insulin stress and longevity signaling pathway
    D Wang
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Cold Spring Harb Symp Quant Biol 69:429-31. 2004
  19. ncbi request reprint Misexpression of the Caenorhabditis elegans miRNA let-7 is sufficient to drive developmental programs
    G D Hayes
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Cold Spring Harb Symp Quant Biol 71:21-7. 2006
    ..elegans and other organisms...
  20. ncbi request reprint A phosphatidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans
    J Z Morris
    Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA
    Nature 382:536-9. 1996
    ..These data suggest that phosphatidylinositol signalling mediated by AGE-1 protein controls lifespan and the dauer diapause decision...
  21. doi request reprint The C. elegans DAF-2 insulin-like receptor is abundantly expressed in the nervous system and regulated by nutritional status
    K D Kimura
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Cold Spring Harb Symp Quant Biol 76:113-20. 2011
    ..The modulation of DAF-2 protein level by nutritional status may constitute an important component in the irreversible commitment to dauer arrest...
  22. ncbi request reprint Temporal regulation of lin-14 by the antagonistic action of two other heterochronic genes, lin-4 and lin-28
    P Arasu
    Department of Molecular Biology, Massachusetts General Hospital, Boston
    Genes Dev 5:1825-33. 1991
    ..Switching off lin-14 expression during the L1 stage is not triggered by the passage of time per se but, rather, is normally dependent on feeding or the feeding-dependent initiation of postembryonic cell division...
  23. pmc DAF-16 recruits the CREB-binding protein coactivator complex to the insulin-like growth factor binding protein 1 promoter in HepG2 cells
    N Nasrin
    Diabetes Research Unit and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 97:10412-7. 2000
    ....
  24. ncbi request reprint Themes from a NASA workshop on gene regulatory processes in development and evolution
    E H Davidson
    Division of Biology 156 29, California Institute of Technology, Pasadena, California 91125, USA
    J Exp Zool 285:104-15. 1999
    ..J. Exp. Zool. (Mol. Dev. Evol. ) 285:104-115, 1999...
  25. ncbi request reprint daf-2, an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans
    K D Kimura
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Science 277:942-6. 1997
    ..Life-span regulation by insulin-like metabolic control is analogous to mammalian longevity enhancement induced by caloric restriction, suggesting a general link between metabolism, diapause, and longevity...
  26. ncbi request reprint Identification of Caenorhabditis elegans genes regulating longevity using enhanced RNAi-sensitive strains
    A V Samuelson
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Cold Spring Harb Symp Quant Biol 72:489-97. 2007
    ..The results of this study reinforce the importance of metabolism, mitochondrial and lysosomal functions, genomic stability, and stress resistance on animal life-span determination...
  27. ncbi request reprint Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA
    A E Pasquinelli
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA
    Nature 408:86-9. 2000
    ..elegans and Drosophila, at 48 hours after fertilization in zebrafish, and in adult stages of annelids and molluscs. The let-7 regulatory RNA may control late temporal transitions during development across animal phylogeny...
  28. ncbi request reprint Regulation of interneuron function in the C. elegans thermoregulatory pathway by the ttx-3 LIM homeobox gene
    O Hobert
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA
    Neuron 19:345-57. 1997
    ..The ttx-3-dependent thermosensory pathway also couples to the temperature-modulated dauer neuroendocrine signaling pathway, showing that ttx-3 specifies AIY thermosensory information processing of both motor and autonomic outputs...
  29. ncbi request reprint Targeted mutations in the Caenorhabditis elegans POU homeo box gene ceh-18 cause defects in oocyte cell cycle arrest, gonad migration, and epidermal differentiation
    D Greenstein
    Department of Molecular Biology, Massachusetts General Hospital, Boston 02114
    Genes Dev 8:1935-48. 1994
    ..Analysis of targeted deletions that disrupt half of the POU domain selectively by deleting either the POUhd or the POUsp alone, indicates that each CEH-18 POU subdomain is sufficient for partial activity in vivo...
  30. ncbi request reprint Food and metabolic signalling defects in a Caenorhabditis elegans serotonin-synthesis mutant
    J Y Sze
    Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA
    Nature 403:560-4. 2000
    ..The action of the C. elegans serotonergic system in metabolic control is similar to mammalian serotonergic input to metabolism and obesity...
  31. pmc The DAF-3 Smad protein antagonizes TGF-beta-related receptor signaling in the Caenorhabditis elegans dauer pathway
    G I Patterson
    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114 USA
    Genes Dev 11:2679-90. 1997
    ..Therefore, daf-3 is unique in that it is antagonized, rather than activated, by a TGF-beta pathway...
  32. ncbi request reprint The Caenorhabditis elegans heterochronic gene lin-14 encodes a nuclear protein that forms a temporal developmental switch
    G Ruvkun
    Department of Molecular Biology, Massachusetts General Hospital, Boston 02114
    Nature 338:313-9. 1989
    ..The normal down-regulation of the lin-14 nuclear protein level encodes a temporal switch between early and late cell fates...
  33. ncbi request reprint The C. elegans PTEN homolog, DAF-18, acts in the insulin receptor-like metabolic signaling pathway
    S Ogg
    Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA
    Mol Cell 2:887-93. 1998
    ..The action of daf-18 in this metabolic control pathway suggests that mammalian PTEN may modulate insulin signaling and may be variant in diabetic pedigrees...
  34. ncbi request reprint The unc-86 gene product couples cell lineage and cell identity in C. elegans
    M Finney
    Department of Genetics, Harvard Medical School, Boston, Massachusetts
    Cell 63:895-905. 1990
    ..unc-86 protein also appears shortly after cell division in the nuclei of particular identified differentiating neurons; at least some of these neurons are nonfunctional in unc-86 mutants...
  35. ncbi request reprint Caenorhabditis elegans has scores of homoeobox-containing genes
    T R Burglin
    Department of Molecular Biology, Massachusetts General Hospital, Boston
    Nature 341:239-43. 1989
    ..We estimate that the number of homoeobox-containing genes in C. elegans is at least 60, constituting approximately 1% of the estimated total number of genes...
  36. ncbi request reprint C. elegans unc-4 gene encodes a homeodomain protein that determines the pattern of synaptic input to specific motor neurons
    D M Miller
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710
    Nature 355:841-5. 1992
    ..The limited effect of the unc-4 null mutation on cell fate may mean that unc-4 regulates the expression of a small number of target genes and that the products of these genes are directly involved in the choice of synaptic partners...
  37. ncbi request reprint Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing
    A Grishok
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 106:23-34. 2001
    ..Our findings suggest that a common processing machinery generates guide RNAs that mediate both RNAi and endogenous gene regulation...
  38. ncbi request reprint The Caenorhabditis elegans orphan nuclear hormone receptor gene nhr-2 functions in early embryonic development
    A E Sluder
    Department of Cellular Biology, University of Georgia, Athens 30602, USA
    Dev Biol 184:303-19. 1997
    ..nhr-2 does not correspond to known mutations mapped to the same genetic interval, and will provide an entry point for further study of a heretofore uncharacterized zygotic gene regulatory pathway...
  39. ncbi request reprint The POU domain: a large conserved region in the mammalian pit-1, oct-1, oct-2, and Caenorhabditis elegans unc-86 gene products
    W Herr
    Cold Spring Harbor Laboratory, New York 11724
    Genes Dev 2:1513-6. 1988