Tom Rapoport

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Generation of nonidentical compartments in vesicular transport systems
    Reinhart Heinrich
    Institute of Biology, Department of Biophysics, Humboldt University, Berlin, D 10115, Germany
    J Cell Biol 168:271-80. 2005
  2. pmc Disulfide bridge formation between SecY and a translocating polypeptide localizes the translocation pore to the center of SecY
    Kurt S Cannon
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 169:219-25. 2005
  3. pmc Unfolded cholera toxin is transferred to the ER membrane and released from protein disulfide isomerase upon oxidation by Ero1
    Billy Tsai
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Cell Biol 159:207-16. 2002
  4. pmc Function of the p97-Ufd1-Npl4 complex in retrotranslocation from the ER to the cytosol: dual recognition of nonubiquitinated polypeptide segments and polyubiquitin chains
    Yihong Ye
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Cell Biol 162:71-84. 2003
  5. pmc Analysis of polypeptide movement in the SecY channel during SecA-mediated protein translocation
    Karl J Erlandson
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 283:15709-15. 2008
  6. pmc Preserving the membrane barrier for small molecules during bacterial protein translocation
    Eunyong Park
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 473:239-42. 2011
  7. pmc Bacterial protein translocation requires only one copy of the SecY complex in vivo
    Eunyong Park
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 198:881-93. 2012
  8. pmc Mutants affecting the structure of the cortical endoplasmic reticulum in Saccharomyces cerevisiae
    W A Prinz
    Department of Cell Biology and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 150:461-74. 2000
  9. ncbi request reprint Mechanisms of Sec61/SecY-mediated protein translocation across membranes
    Eunyong Park
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Biophys 41:21-40. 2012
  10. pmc A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein
    M M Rolls
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 146:29-44. 1999

Research Grants

Collaborators

Detail Information

Publications71

  1. pmc Generation of nonidentical compartments in vesicular transport systems
    Reinhart Heinrich
    Institute of Biology, Department of Biophysics, Humboldt University, Berlin, D 10115, Germany
    J Cell Biol 168:271-80. 2005
    ..With nonidentical compartments established in this way, the localization and cellular transport of cargo proteins can be explained simply by their affinity for coats...
  2. pmc Disulfide bridge formation between SecY and a translocating polypeptide localizes the translocation pore to the center of SecY
    Kurt S Cannon
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 169:219-25. 2005
    ..This suggests that the channel makes only limited contact with a translocating polypeptide, thus minimizing the energy required for translocation...
  3. pmc Unfolded cholera toxin is transferred to the ER membrane and released from protein disulfide isomerase upon oxidation by Ero1
    Billy Tsai
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Cell Biol 159:207-16. 2002
    ..Taken together, our results identify Ero1 as the enzyme mediating the release of unfolded CT from PDI and characterize an additional step in retrotranslocation of the toxin...
  4. pmc Function of the p97-Ufd1-Npl4 complex in retrotranslocation from the ER to the cytosol: dual recognition of nonubiquitinated polypeptide segments and polyubiquitin chains
    Yihong Ye
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Cell Biol 162:71-84. 2003
    ....
  5. pmc Analysis of polypeptide movement in the SecY channel during SecA-mediated protein translocation
    Karl J Erlandson
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 283:15709-15. 2008
    ..The channel itself does not bind the polypeptide chain but provides "friction" that minimizes backsliding when ADP-bound SecA resets to "grab" the next segment of the substrate...
  6. pmc Preserving the membrane barrier for small molecules during bacterial protein translocation
    Eunyong Park
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 473:239-42. 2011
    ..The structural conservation of the channel in all organisms indicates that this may be a universal mechanism by which the membrane barrier is maintained during protein translocation...
  7. pmc Bacterial protein translocation requires only one copy of the SecY complex in vivo
    Eunyong Park
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 198:881-93. 2012
    ..Mutations that abolish the interaction between SecY molecules still supported viability of E. coli. These results show that a single SecY molecule is sufficient for protein translocation...
  8. pmc Mutants affecting the structure of the cortical endoplasmic reticulum in Saccharomyces cerevisiae
    W A Prinz
    Department of Cell Biology and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 150:461-74. 2000
    ..Our data suggest that both trafficking between the ER and Golgi complex and ribosome targeting are important for maintaining ER structure, and that proper ER structure may be required to maintain mitochondrial structure...
  9. ncbi request reprint Mechanisms of Sec61/SecY-mediated protein translocation across membranes
    Eunyong Park
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Biophys 41:21-40. 2012
    ....
  10. pmc A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein
    M M Rolls
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 146:29-44. 1999
    ..Thus, nurim is a new type of NE membrane protein that is localized to the NE by a distinct mechanism...
  11. ncbi request reprint Protein transport across the endoplasmic reticulum membrane
    Tom A Rapoport
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    FEBS J 275:4471-8. 2008
    ..Structural and biochemical data suggest mechanisms that enable the channel to function with different partners, to open across the membrane and to release laterally hydrophobic segments of membrane proteins into lipid...
  12. ncbi request reprint Membrane-protein integration and the role of the translocation channel
    Tom A Rapoport
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Trends Cell Biol 14:568-75. 2004
    ..We also discuss how the channel would prevent small molecules from crossing the lipid bilayer while it is integrating proteins...
  13. ncbi request reprint Protein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes
    Tom A Rapoport
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 450:663-9. 2007
    ..Structural, genetic and biochemical data show how the channel opens across the membrane, releases hydrophobic segments of membrane proteins laterally into lipid, and maintains the membrane barrier for small molecules...
  14. pmc A preliminary report on my life in science
    Tom A Rapoport
    Department of Cell Biology, Harvard Medical School, and Howard Hughes Medical Institute, Boston, MA 02115, USA
    Mol Biol Cell 21:3770-2. 2010
    ..Although elements of my story are unique, the main points are general: don't be afraid to start something new; it pays to be persistent; and science is a passion--if it feels like fun, you've probably got it right...
  15. pmc Cross-linked SecA dimers are not functional in protein translocation
    Eran Or
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    FEBS Lett 581:2616-20. 2007
    ..In contrast, upon reduction of the disulfide bridges, the resulting monomers regain these activities. These data support the notion that dissociation of SecA dimers into monomers occurs during protein translocation...
  16. pmc Mapping polypeptide interactions of the SecA ATPase during translocation
    Benedikt W Bauer
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 106:20800-5. 2009
    ..These results define the polypeptide path during SecA-mediated protein translocation and suggest a mechanism by which ATP hydrolysis by SecA is used to move a polypeptide chain through the SecY channel...
  17. pmc Interactions between Sec complex and prepro-alpha-factor during posttranslational protein transport into the endoplasmic reticulum
    Kathrin Plath
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Biol Cell 15:1-10. 2004
    ..These data suggest that the channel pore is lined by several transmembrane segments, which have no significant affinity for the translocating polypeptide chain...
  18. pmc Recognition of an ERAD-L substrate analyzed by site-specific in vivo photocrosslinking
    Ann Marie Stanley
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    FEBS Lett 585:1281-6. 2011
    ..Hrd3p may thus be the first substrate-recognizing component. Der1p appears to have a role in a pathway that is parallel to that involving Hrd3p...
  19. ncbi request reprint Ribosome binding of a single copy of the SecY complex: implications for protein translocation
    Jean François Ménétret
    Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany Street, Boston, MA 02118 2526, USA
    Mol Cell 28:1083-92. 2007
    ..We also show that point mutations of basic residues within either loop abolish ribosome binding. We suggest that SecY binds to this primary site on the ribosome and subsequently captures and translocates the nascent chain...
  20. ncbi request reprint Membrane proteins of the endoplasmic reticulum induce high-curvature tubules
    Junjie Hu
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Science 319:1247-50. 2008
    ..Tubules made in vitro were narrower than normal ER tubules, due to a higher concentration of tubule-inducing proteins. The shape and oligomerization of the "morphogenic" proteins could explain the formation of the tubular ER...
  21. ncbi request reprint A role for the two-helix finger of the SecA ATPase in protein translocation
    Karl J Erlandson
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 455:984-7. 2008
    ....
  22. ncbi request reprint The bacterial ATPase SecA functions as a monomer in protein translocation
    Eran Or
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:9097-105. 2005
    ..Disulfide cross-linking in intact cells showed that mutant SecA is monomeric and that even its parental dimeric form is dissociated. Our results suggest that SecA functions as a monomer during protein translocation in vivo...
  23. pmc The endoplasmic reticulum membrane is permeable to small molecules
    Sylvie Le Gall
    Department of Cell Biology, Howard Hughes Medical Institute Harvard Medical School, Boston, Massachusetts 02115 6091, USA
    Mol Biol Cell 15:447-55. 2004
    ..These data indicate that the ER membrane is significantly more leaky than other cellular membranes, a property that may be required for protein folding and other functions of the ER...
  24. ncbi request reprint Distinct ubiquitin-ligase complexes define convergent pathways for the degradation of ER proteins
    Pedro Carvalho
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 126:361-73. 2006
    ..All three pathways converge at the Cdc48p ATPase complex. These results lead to a unifying concept for ERAD that may also apply to mammalian cells...
  25. ncbi request reprint Role of p97 AAA-ATPase in the retrotranslocation of the cholera toxin A1 chain, a non-ubiquitinated substrate
    Michael Kothe
    GI Cell Biology, Children s Hospital Boston, the Harvard Digestive Diseases Center, Harvard Medical School, Massachusetts 02115, USA
    J Biol Chem 280:28127-32. 2005
    ..These results suggest that p97 does not provide the primary driving force for extracting the A1 chain from the endoplasmic reticulum, a finding that is consistent with a requirement for polyubiquitination in p97 function...
  26. ncbi request reprint Structural insight into the protein translocation channel
    William M Clemons
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Curr Opin Struct Biol 14:390-6. 2004
    ..The electron microscopy and X-ray results together suggest that only one copy of the SecY or Sec61 complex within an oligomer translocates a polypeptide chain at any given time...
  27. pmc Conformational flexibility and peptide interaction of the translocation ATPase SecA
    Jochen Zimmer
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Mol Biol 394:606-12. 2009
    ..5 A resolution. This structure shows that the peptide augments the highly conserved beta-sheet at the back of the clamp. Taken together, these structures suggest a mechanism by which ATP hydrolysis can lead to polypeptide translocation...
  28. ncbi request reprint Protein translocation is mediated by oligomers of the SecY complex with one SecY copy forming the channel
    Andrew R Osborne
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 129:97-110. 2007
    ..Oligomeric channels with only one active pore likely mediate protein translocation in all organisms...
  29. pmc Cooperation of transmembrane segments during the integration of a double-spanning protein into the ER membrane
    Sven U Heinrich
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    EMBO J 22:3654-63. 2003
    ..These data suggest that the exit of a weak TM segment from the Sec61 channel into the lipid phase can be facilitated by its interaction with a previously integrated strong and stabilizing TM anchor...
  30. ncbi request reprint Structure of a complex of the ATPase SecA and the protein-translocation channel
    Jochen Zimmer
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 455:936-43. 2008
    ..SecA binding generates a 'window' at the lateral gate of the SecY channel and it displaces the plug domain, preparing the channel for signal sequence binding and channel opening...
  31. pmc A large conformational change of the translocation ATPase SecA
    Andrew R Osborne
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 101:10937-42. 2004
    ..We propose that the open form of SecA represents an activated state...
  32. ncbi request reprint Structure of the mammalian ribosome-channel complex at 17A resolution
    David Gene Morgan
    Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany St, Boston, MA 02118 2526, USA
    J Mol Biol 324:871-86. 2002
    ..These data suggest that the flow of small molecules across the membrane may be impeded by the channel itself, rather than the ribosome-channel junction...
  33. ncbi request reprint Architecture of the ribosome-channel complex derived from native membranes
    Jean François Ménétret
    Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany St, Boston, MA 02118 2526, USA
    J Mol Biol 348:445-57. 2005
    ..Thus, we suggest that a single Sec61 complex may form a conduit for translocating polypeptides, while three copies of Sec61 play a structural role or recruit accessory factors such as TRAP...
  34. ncbi request reprint The plug domain of the SecY protein stabilizes the closed state of the translocation channel and maintains a membrane seal
    Weikai Li
    Howard Hughes Medical Institute, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell 26:511-21. 2007
    ..Our data show that the plug domain is required to maintain a closed state of the channel and suggest a mechanism for channel gating...
  35. pmc Dissociation of the dimeric SecA ATPase during protein translocation across the bacterial membrane
    Eran Or
    Department of Cell Biology, Harvard Medical School, HHMI, Boston, MA 02115, USA
    EMBO J 21:4470-9. 2002
    ..Since SecA contains all characteristic motifs of a certain class of monomeric helicases, and since mutations in residues shared with the helicases abolish its translocation activity, SecA may function in a similar manner...
  36. pmc Gangliosides that associate with lipid rafts mediate transport of cholera and related toxins from the plasma membrane to endoplasmic reticulm
    Yukako Fujinaga
    GI Cell Biology, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Biol Cell 14:4783-93. 2003
    ..Our results explain why LTIIb does not cause disease in humans and suggest that gangliosides with high affinity for lipid rafts may provide a general vehicle for the transport of toxins to the ER...
  37. ncbi request reprint Mechanisms shaping the membranes of cellular organelles
    Yoko Shibata
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Cell Dev Biol 25:329-54. 2009
    ..Mechanisms affecting local membrane curvature may also shape peripheral ER sheets and the nuclear envelope as well as mitochondria and caveolae...
  38. pmc Vitamin K epoxide reductase prefers ER membrane-anchored thioredoxin-like redox partners
    Sol Schulman
    Department of Cell Biology, Harvard Medical School, Howard Hughes Medical Institute, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 107:15027-32. 2010
    ..Taken together, our results demonstrate that human VKOR employs the same electron transfer pathway as its bacterial homologs and that VKORs generally prefer membrane-bound Trx-like redox partners...
  39. ncbi request reprint Crystal structure of an unusual thioredoxin protein with a zinc finger domain
    Jiqing Ye
    Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 282:34945-51. 2007
    ..On the basis of structural similarity to the zinc fingers in Npl4 and Vps36, we propose that the N-terminal zinc finger of thioredoxin-2 mediates protein-protein interactions, possibly with its substrates or chaperones...
  40. ncbi request reprint Crystal structure of the long-chain fatty acid transporter FadL
    Bert van den Berg
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Science 304:1506-9. 2004
    ..The structures suggest that hydrophobic compounds bind to multiple sites in FadL and use a transport mechanism that involves spontaneous conformational changes in the hatch...
  41. ncbi request reprint RecA-like motor ATPases--lessons from structures
    Jiqing Ye
    Department of Cell Biology, Harvard Medical School, HHMI, 240 Longwood Ave, LHRRB 613, Boston, MA 02115, USA
    Biochim Biophys Acta 1659:1-18. 2004
    ..The structures determined for different RecA-like motor ATPases begin to reveal how they move macromolecules...
  42. ncbi request reprint A membrane protein complex mediates retro-translocation from the ER lumen into the cytosol
    Yihong Ye
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 429:841-7. 2004
    ..Derlin-1 interacts with US11, a virally encoded ER protein that specifically targets MHC class I heavy chains for export from the ER, as well as with VIMP, a novel membrane protein that recruits the p97 ATPase and its cofactor...
  43. ncbi request reprint X-ray structure of a protein-conducting channel
    Bert van den Berg
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:36-44. 2004
    ....
  44. ncbi request reprint Protein translocation by the Sec61/SecY channel
    Andrew R Osborne
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Cell Dev Biol 21:529-50. 2005
    ..We review these translocation mechanisms, relating biochemical and genetic observations to the structures of the protein-conducting channel and its binding partners...
  45. pmc Structure of a bacterial homologue of vitamin K epoxide reductase
    Weikai Li
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 463:507-12. 2010
    ..Our results have implications for the mechanism of the mammalian VKOR and explain how mutations can cause resistance to the VKOR inhibitor warfarin, the most commonly used oral anticoagulant...
  46. pmc Role of ubiquitination in retro-translocation of cholera toxin and escape of cytosolic degradation
    Chiara Rodighiero
    GI Cell Biology, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    EMBO Rep 3:1222-7. 2002
    ....
  47. ncbi request reprint Retro-translocation of proteins from the endoplasmic reticulum into the cytosol
    Billy Tsai
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Nat Rev Mol Cell Biol 3:246-55. 2002
    ..This retro-translocation pathway has been co-opted by certain viruses, and by plant and bacterial toxins. The mechanism of retro-translocation is still mysterious, but several aspects of this process are now being unravelled...
  48. ncbi request reprint Polyubiquitin serves as a recognition signal, rather than a ratcheting molecule, during retrotranslocation of proteins across the endoplasmic reticulum membrane
    Dennis Flierman
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:34774-82. 2003
    ..These data suggest that polyubiquitin does not serve as a ratcheting molecule. Rather, it may serve as a recognition signal for the p97-Ufd1-Npl4 complex, a component implicated in the movement of substrate into the cytosol...
  49. ncbi request reprint A class of membrane proteins shaping the tubular endoplasmic reticulum
    Gia K Voeltz
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 124:573-86. 2006
    ..The simultaneous absence of the reticulons and Yop1p in S. cerevisiae results in disrupted tubular ER. We propose that these "morphogenic" proteins partition into and stabilize highly curved ER membrane tubules...
  50. ncbi request reprint A novel dimer interface and conformational changes revealed by an X-ray structure of B. subtilis SecA
    Jochen Zimmer
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA
    J Mol Biol 364:259-65. 2006
    ..subtilis SecA suggests that small changes in the nucleotide binding domains could be amplified via helix 1 of the helical scaffold domain (hsd) to generate larger movements of the domains involved in polypeptide binding...
  51. pmc Retrotranslocation of a misfolded luminal ER protein by the ubiquitin-ligase Hrd1p
    Pedro Carvalho
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 143:579-91. 2010
    ..Our results suggest a model for how Hrd1p promotes polypeptide movement through the ER membrane...
  52. pmc The reticulon and DP1/Yop1p proteins form immobile oligomers in the tubular endoplasmic reticulum
    Yoko Shibata
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 283:18892-904. 2008
    ..We propose that oligomerization of the reticulons and DP1/Yop1p is important for both their localization to the tubular domains of the ER and for their ability to form tubules...
  53. pmc Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome
    Jean François Ménétret
    Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany Street, Boston, MA 02118 2526, USA
    Structure 16:1126-37. 2008
    ..Hence, this copy of Sec61 is positioned to capture and translocate the nascent chain. Finally, we show that mammalian and bacterial ribosome-channel complexes have similar architectures...
  54. ncbi request reprint Rough sheets and smooth tubules
    Yoko Shibata
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 126:435-9. 2006
    ..Key proteins may drive the formation of these structural morphologies, which in turn could generate the rough and smooth functional domains of the ER...
  55. pmc Ribosome binding to and dissociation from translocation sites of the endoplasmic reticulum membrane
    Julia Schaletzky
    Department of Cell Biology and Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Mol Biol Cell 17:3860-9. 2006
    ..This could explain how RNC-SRP complexes can overcome the competition by nontranslating ribosomes...
  56. pmc Targeting of rough endoplasmic reticulum membrane proteins and ribosomes in invertebrate neurons
    Melissa M Rolls
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Biol Cell 13:1778-91. 2002
    ..Ribosomes were also concentrated in the cell body, suggesting they may be in part responsible for targeting RER membrane proteins...
  57. pmc The ER-associated degradation component Der1p and its homolog Dfm1p are contained in complexes with distinct cofactors of the ATPase Cdc48p
    Veit Goder
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    FEBS Lett 582:1575-80. 2008
    ..These data suggest distinct functions for the Der1p and Dfm1p complexes...
  58. pmc A class of dynamin-like GTPases involved in the generation of the tubular ER network
    Junjie Hu
    Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 138:549-61. 2009
    ..Since atlastin-1 mutations cause a common form of hereditary spastic paraplegia, we suggest ER-shaping defects as a neuropathogenic mechanism...
  59. pmc Gangliosides are receptors for murine polyoma virus and SV40
    Billy Tsai
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    EMBO J 22:4346-55. 2003
    ..Lipid binding sites for polyoma are shown to be present in rough ER membranes, suggesting that the virus travel with the ganglioside(s) from the plasma membranes to the ER...
  60. pmc Structural organization of the endoplasmic reticulum
    Gia K Voeltz
    Department of Cell Biology and Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    EMBO Rep 3:944-50. 2002
    ..Throughout these rearrangements the ER maintains its basic structure. How this is accomplished remains mysterious, but some insight has been gained from in vitro systems...
  61. pmc The ATPase SpoIIIE transports DNA across fused septal membranes during sporulation in Bacillus subtilis
    Briana M Burton
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 131:1301-12. 2007
    ..These data support a model for DNA transport in which the transmembrane segments of FtsK/SpoIIIE form linked DNA-conducting channels across the two lipid bilayers of the septum...
  62. pmc Mechanisms determining the morphology of the peripheral ER
    Yoko Shibata
    Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell 143:774-88. 2010
    ..These proteins may generate sheets by stabilizing the high curvature of edges...
  63. pmc The signal sequence coding region promotes nuclear export of mRNA
    Alexander F Palazzo
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Biol 5:e322. 2007
    ..The discovery of an SSCR-mediated pathway explains the previously noted amino acid bias in signal sequences and suggests a link between nuclear export and membrane targeting of mRNAs...
  64. ncbi request reprint A novel centrosome-associated protein with affinity for microtubules
    Pascal A Stein
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115 6091, USA
    J Cell Sci 115:3389-402. 2002
    ..Tight binding to microtubules in interphase appears to require an oligomeric state of MIR1, and phosphorylation in mitosis at predicted cyclin-dependent kinase (cdk) sites weakens the interaction...
  65. ncbi request reprint Determining the conductance of the SecY protein translocation channel for small molecules
    Sapar M Saparov
    Institut fuer Biophysik, Johannes Kepler Universitaet Linz, Linz, Austria
    Mol Cell 26:501-9. 2007
    ..These data show that the resting channel on its own forms a barrier for small molecules, with both the pore ring and the plug required for the seal; channel opening requires movement of the plug...
  66. ncbi request reprint Three-dimensional structure of the bacterial protein-translocation complex SecYEG
    Cécile Breyton
    Max Planck Institut fur Biophysik, Abteilung Strukturbiologie, Heinrich Hoffmann Strasse 7, 60528 Frankfurt am Main, Germany
    Nature 418:662-5. 2002
    ..In the centre of the dimer we observe a 16 x 25 A cavity closed on the periplasmic side by two highly tilted transmembrane helices. This may represent the closed state of the protein-conducting channel...
  67. pmc Recruitment of the p97 ATPase and ubiquitin ligases to the site of retrotranslocation at the endoplasmic reticulum membrane
    Yihong Ye
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 102:14132-8. 2005
    ....
  68. ncbi request reprint Mathematical models of protein kinase signal transduction
    Reinhart Heinrich
    Institute of Biology, Department of Biophysics, Humboldt University, Berlin, Germany
    Mol Cell 9:957-70. 2002
    ..Our models also explain how different agonists can evoke transient or sustained signaling of the same pathway and provide a rationale for signaling pathway design...
  69. pmc Profile of Tom A. Rapoport
    Tinsley H Davis
    Proc Natl Acad Sci U S A 102:14129-31. 2005
  70. pmc E2-25K mediates US11-triggered retro-translocation of MHC class I heavy chains in a permeabilized cell system
    Dennis Flierman
    Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
    Proc Natl Acad Sci U S A 103:11589-94. 2006
    ..We conclude that in permeabilized, US11-expressing cells polyubiquitination of the HC substrate can be catalyzed by E2-25K, perhaps in cooperation with the ligase MARCHVII/axotrophin...

Research Grants1

  1. POSTTRANSLATIONAL PROTEIN TRANSPORT INTO YEAST ER
    Tom Rapoport; Fiscal Year: 2003
    ..It will test the hypothesis that this protein works as a molecular ratchet rather than a machine that "pulls" the protein through the membrane. ..