Genomes and Genes
B H Paw
Affiliation: Harvard University
- Cloning of the zebrafish retsina blood mutation: a genetic model for dyserythropoiesis and erythroid cytokinesisB H Paw
Division of Hematology Oncology, Department of Pediatric Oncology, Children s Hospital, Dana Farber Cancer Institute, 320 Longwood Avenue, Enders 7, Boston, Massachusetts 02115, USA
Blood Cells Mol Dis 27:62-4. 2001
- Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporterA Donovan
Department of Medicine, Children s Hospital and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
Nature 403:776-81. 2000..We propose that Ferroportin1 function may be perturbed in mammalian disorders of iron deficiency or overload...
- Hereditary spherocytosis in zebrafish riesling illustrates evolution of erythroid beta-spectrin structure, and function in red cell morphogenesis and membrane stabilityE C Liao
Division of Hematology Oncology, Children s Hospital, Department of Pediatrics and Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
Development 127:5123-32. 2000..Further, we propose that the distinct mammalian erythroid morphology may have evolved from specific modifications of Sptb structure and function...
- Myelopoiesis in the zebrafish, Danio rerioC M Bennett
Department of Pediatric Oncology, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
Blood 98:643-51. 2001..The morphologic and molecular genetic evidence presented here supports the zebrafish as an informative model system for the study of normal and aberrant human myelopoiesis. (Blood. 2001;98:643-651)..
- Positional cloning of the zebrafish sauternes gene: a model for congenital sideroblastic anaemiaA Brownlie
Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Genet 20:244-50. 1998..As mutations in ALAS2 cause congenital sideroblastic anaemia (CSA) in humans, sau represents the first animal model of this disease...
- Zebrafish: a genetic model for vertebrate organogenesis and human disordersGabriele E Ackermann
Division of Hematology Oncology, Brigham and Women s Hospital, Harvard Medical School, 221 Longwood Avenue, Boston, MA 02115, USA
Front Biosci 8:d1227-53. 2003..This review updates our knowledge of heart, vessel, blood and kidney organogenesis in zebrafish and extrapolates our insights to human disorders by assessing common genetic pathways...
- Zebrafish ae2.2 encodes a second slc4a2 anion exchangerBoris E Shmukler
Molecular and Vascular Medicine and Renal Units, Beth Israel Deaconess Medical Center E RW763, 330 Brookline Ave, Boston, MA 02215, USA
Am J Physiol Regul Integr Comp Physiol 294:R1081-91. 2008..1 mRNA, or of both with nontoxic or minimally toxic levels of N-morpholino oligomers produced no grossly detectable morphological phenotype, and preserved normal structure of the head and the pronephric duct at 24 h postfertilization...
- Cell-specific mitotic defect and dyserythropoiesis associated with erythroid band 3 deficiencyBarry H Paw
Department of Medicine, Division of Hematology Oncology, Children s Hospital, Boston, Massachusetts, USA
Nat Genet 34:59-64. 2003..1R-binding domains. Our report establishes an evolutionarily conserved role for band 3 in erythroid-specific cell division and illustrates the concept of cell-specific adaptation for mitosis...
- Zebrafish slc4a2/ae2 anion exchanger: cDNA cloning, mapping, functional characterization, and localizationBoris E Shmukler
Molecular Medicine and Renal Units, Beth Israel Deaconess Med Ctr E RW763, 330 Brookline Ave, Boston, MA 02215, USA
Am J Physiol Renal Physiol 289:F835-49. 2005..These results will inform tests of the role of Ae2 in zebrafish kidney development and function...
- A GATA-1-regulated microRNA locus essential for erythropoiesisLouis C Dore
Division of Hematology, Children s Hospital of Philadelphia and The University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 105:3333-8. 2008..These results reveal a miRNA locus that is required for erythropoiesis and uncover a new regulatory axis through which GATA-1 controls this process...
- Haem homeostasis is regulated by the conserved and concerted functions of HRG-1 proteinsAbbhirami Rajagopal
Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742, USA
Nature 453:1127-31. 2008..Thus, uncovering the mechanisms of haem transport in C. elegans may provide insights into human disorders of haem metabolism and reveal new drug targets for developing anthelminthics to combat worm infestations...
- montalcino, A zebrafish model for variegate porphyriaKimberly A Dooley
Division of Hematology Oncology, Children s Hospital, Howard Hughes Medical Institute, Boston, MA 02115, USA
Exp Hematol 36:1132-42. 2008..The objective of this study was to identify the defective gene and characterize the phenotype of the zebrafish mutant...
- Network of coregulated spliceosome components revealed by zebrafish mutant in recycling factor p110Nikolaus S Trede
Institute of Biochemistry, Justus Liebig University, D 35392 Giessen, Germany
Proc Natl Acad Sci U S A 104:6608-13. 2007..Together, our data demonstrate that a mutation in a general splicing factor can lead to distinct defects in organ development and cause disease...
- Mitoferrin is essential for erythroid iron assimilationGeorge C Shaw
Division of Hematology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 440:96-100. 2006..Our data show that mfrn functions as the principal mitochondrial iron importer essential for haem biosynthesis in vertebrate erythroblasts...
- Vascular endothelial growth factor receptor signaling is required for cardiac valve formation in zebrafishYou Mie Lee
Vascular Biology Program, Department of Surgery, Children s Hospital, Boston, Massachusetts 02115, USA
Dev Dyn 235:29-37. 2006..Taken together, these data demonstrate a novel function for VEGF-Rs in the endocardial endothelium of the developing cardiac valve...
- Deficiency of glutaredoxin 5 reveals Fe-S clusters are required for vertebrate haem synthesisRebecca A Wingert
Stem Cell Program and Division Hematology Oncology Children s Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 436:1035-39. 2005..These findings uncover a connection between haem biosynthesis and Fe-S clusters, indicating that haemoglobin production in the differentiating red cell is regulated through Fe-S cluster assembly...
- Identification of ZBP-89 as a novel GATA-1-associated transcription factor involved in megakaryocytic and erythroid developmentAndrew J Woo
Department of Pediatric Oncology, Children s Hospital Boston Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Cell Biol 28:2675-89. 2008....
- Analysis of thrombocyte development in CD41-GFP transgenic zebrafishHui Feng Lin
Hematology Division, Department of Medicine, Brigham and Women s Hospital, 75 Francis St, Boston, MA 02115, USA
Blood 106:3803-10. 2005..These studies have shown that it is possible to identify thrombocytes, thrombocyte precursors, and, possibly, early hematopoietic stem cells in zebrafish embryos and track their proliferation and maturation...
- Zebrafish as a model of human hematologic disordersEbrahim Shafizadeh
Division of Hematology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
Curr Opin Hematol 11:255-61. 2004....
- Zebrafish SPI-1 (PU.1) marks a site of myeloid development independent of primitive erythropoiesis: implications for axial patterningGraham J Lieschke
Ludwig Institute for Cancer Research, The Royal Melbourne Hospital, Parkville, Victoria, 3050, Australia
Dev Biol 246:274-95. 2002....
- Characterization of zebrafish merlot/chablis as non-mammalian vertebrate models for severe congenital anemia due to protein 4.1 deficiencyEbrahim Shafizadeh
Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912 USA
Development 129:4359-70. 2002..This work presents merlot/chablis as the first characterized non-mammalian vertebrate models of hereditary anemia due to a defect in protein 4.1R integrity...
- The zebrafish mutant gene chardonnay (cdy) encodes divalent metal transporter 1 (DMT1)Adriana Donovan
Department of Hematology Oncology, and Howard Hughes Medical Institute, Children s Hospital, Boston, MA 02115, USA
Blood 100:4655-9. 2002..Our studies further demonstrate the conservation of iron metabolism in vertebrates and suggest the existence of an alternative pathway of intestinal and red blood cell iron uptake...
- Characterization of embryonic globin genes of the zebrafishAlison Brownlie
Division of Hematology Oncology, Children s Hospital, Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Dev Biol 255:48-61. 2003..Our studies are the first to systematically evaluate the embryonic globins in the zebrafish and will ultimately be useful in evaluating zebrafish mutants with defects in hemoglobin production and switching...
- The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fishAlister C Ward
Centre for Cellular and Molecular Biology, School of Biological and Chemical Sciences, Deakin University, Burwood, Victoria, Australia
Blood 102:3238-40. 2003..These TG(spi1:EGFP)pA301 transgenic zebrafish represent a valuable tool for further studies of myeloid development and its perturbation...
- Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutantsDavid Traver
Children s Hospital Boston and the Howard Hughes Medical Institute, 320 Longwood Avenue, Enders 720, Boston, Massachusetts 02115, USA
Nat Immunol 4:1238-46. 2003..Direct visualization of fluorescent donor cells in embryonic recipients allows engraftment and homing events to be imaged in real time. These results provide a cellular context in which to study the genetics of hematopoiesis...
- Cloning and characterization of an Mx gene and its corresponding promoter from the zebrafish, Danio rerioStephen M Altmann
Department of Biochemistry, Microbiology, and Molecular Biology, University of Maine, Orono, ME 04469, USA
Dev Comp Immunol 28:295-306. 2004....
- The pu.1 promoter drives myeloid gene expression in zebrafishKarl Hsu
Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
Blood 104:1291-7. 2004..These transgenic lines will be useful to dissect the cellular and molecular control of myeloid cell differentiation, and this promoter fragment may prove useful in the development of zebrafish models of acute myeloid leukemia...
- Carboxypeptidase A5 identifies a novel mast cell lineage in the zebrafish providing new insight into mast cell fate determinationJ Tristan Dobson
IWK Health Centre, Dalhousie University, Halifax, NS, USA
Blood 112:2969-72. 2008..1, but not gata-1 or fog-1, are necessary for early MC development. These studies validate the zebrafish as an in vivo tool for studying MC ontogeny and function with future capacity for modeling human MC diseases...