Genomes and Genes
Mark A Parker
Affiliation: Harvard University
- Primary culture and plasmid electroporation of the murine organ of CortiMark Parker
Department of Otology and Laryngology, Harvard Medical School, USA
J Vis Exp . 2010..This method provides an improvement over other published methods because it provides reproducible, unambiguous, and stepwise directions for the isolation, microdissection, and primary culture of the organ of Corti...
- Neural stem cells injected into the sound-damaged cochlea migrate throughout the cochlea and express markers of hair cells, supporting cells, and spiral ganglion cellsMark A Parker
Department of Communication Sciences and Disorders, Emerson College, Boston, MA 02114, USA
Hear Res 232:29-43. 2007..Moreover, they suggest that cells of this neural stem cell line may derive some information needed from the microenvironment of the cochlea to differentiate into replacement cells in the cochlea...
- Biotechnology in the treatment of sensorineural hearing loss: foundations and future of hair cell regenerationMark A Parker
Emerson College, Boston, MA, USA
J Speech Lang Hear Res 54:1709-31. 2011..Next, the author presents a review of stem cell and gene therapy and provides a critical appraisal of their application to hair cell regeneration. The methodologies used in these approaches are highlighted...
- TAK1 expression in the cochlea: a specific marker for adult supporting cellsMark A Parker
Department of Communication Sciences and Disorders, Emerson College, Boston, MA, USA
J Assoc Res Otolaryngol 12:471-83. 2011..While the role of TAK1 in the inner ear is unclear, TAK1 expression may be used as a novel marker for specific sub-populations of supporting cells...
- Expression profile of an operationally-defined neural stem cell cloneMark A Parker
Department of Otolaryngology, EN41, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
Exp Neurol 194:320-32. 2005..Furthermore, when operational definitions are employed, a common set of stem-like genes does emerge across both embryonic and somatic stem cells of various organ systems, including the nervous system...
- Regeneration and replacement in the vertebrate inner earJonathan I Matsui
Laboratory for Cellular and Molecular Hearing Research, Department of Otolaryngology, Children s Hospital, Boston, MA 02115, USA
Drug Discov Today 10:1307-12. 2005..Current research efforts are focusing on gene manipulation, gene therapy and stem cell transplantation for repairing or replacing damaged mammalian cochlear hair cells, which could lead to therapies for treating deafness in humans...
- The potential use of stem cells for cochlear repairMark A Parker
Department of Otology and Laryngology, Harvard Medical School, Boston, Mass, USA
Audiol Neurootol 9:72-80. 2004..The potential use of several types of stem cells, including embryonic, neural and hematopoietic stem cells, as agents for cochlear repair is examined...
- Cell contact-dependent mechanisms specify taste bud pattern during a critical period early in embryonic developmentMark A Parker
Department of Cell and Developmental Biology and Rocky Mountain Taste and Smell Center, University of Colorado Health Sciences Center, Denver, 80262, USA
Dev Dyn 230:630-42. 2004..Furthermore, our findings are consistent with specification of taste buds by means of lateral inhibitory signaling, which we hypothesize results from cell contact-dependent or short-range diffusible signals...