Genomes and Genes
Affiliation: Harvard University
- Single-handed recognition of a sorting traffic motif by the GGA proteinsTom Kirchhausen
Nat Struct Biol 9:241-4. 2002
- Use of dynasore, the small molecule inhibitor of dynamin, in the regulation of endocytosisTom Kirchhausen
Department of Cell Biology, Harvard Medical School, and IDI Immune Research Institute, Boston, Massachusetts, USA
Methods Enzymol 438:77-93. 2008..Here we present a detailed synthesis protocol for dynasore, and describe a series of experiments used to analyze the inhibitory effects of dynasore on dynamin in vitro and to study the effects of dynasore on endocytosis in cells...
- Clathrin adaptors really adaptTom Kirchhausen
Department of Cell Biology, Harvard Medical School and The Center for Blood Research, Boston, MA 02115, USA
Cell 109:413-6. 2002..A paper in this issue of Cell describes the atomic structure of the adaptor-protein 2 (AP-2) core, the portion that makes contacts with the membrane and cytosolic tails of cargo proteins...
- Dynamics of intracellular clathrin/AP1- and clathrin/AP3-containing carriersComert Kural
Department of Cell Biology, Harvard Medical School, Boston and Program in Cellular and Molecular Medicine at Boston Children s Hospital, Boston, MA 02115, USA
Cell Rep 2:1111-9. 2012..This observation suggests that an alternative budding mechanism, yet to be discovered, is responsible for the scission step of clathrin/AP1 and clathrin/AP3 carriers...
- Clathrin-mediated endocytosis persists during unperturbed mitosisSilvia K Tacheva-Grigorova
Program in Cellular and Molecular Medicine, Boston Children s Hospital, Boston, MA 02115, USA
Cell Rep 4:659-68. 2013..Thus, our data show that endocytosis is unaffected during all stages of natural cell division. ..
- The small molecule dispergo tubulates the endoplasmic reticulum and inhibits exportLei Lu
Department of Cell Biology, Harvard Medical School, MA, USA
Mol Biol Cell 24:1020-9. 2013..Because dispergo is the first small molecule that reversibly tubulates the ER and inhibits its export function, it will be useful in studying these complex processes...
- The first five seconds in the life of a clathrin-coated pitEmanuele Cocucci
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Cell 150:495-507. 2012..This objective picture of coated pit initiation also shows that methods outlined here will be broadly useful for studies of dynamic assemblies in living cells...
- Imaging endocytic clathrin structures in living cellsTom Kirchhausen
Harvard Medical School Immune Disease Institute, W Alpert Building Room 128, Boston, MA 02115, USA
Trends Cell Biol 19:596-605. 2009..Here, I outline descriptions of coat assembly in different circumstances that are consistent with what is known from X-ray crystallography and electron microscopy...
- Membrane dynamics of dividing cells imaged by lattice light-sheet microscopyFrançois Aguet
Department of Cell Biology, Harvard Medical School, Boston, MA 02115
Mol Biol Cell 27:3418-3435. 2016..These applications demonstrate the advantage of lattice light-sheet microscopy and enable a new standard for imaging membrane dynamics in single cells and multicellular assemblies...
- Bending membranesTom Kirchhausen
Department of Cell Biology, Harvard Medical School and Program in Cellular and Molecular Medicine at Boston Children s Hospital, Boston, Massachusetts 02115, USA
Nat Cell Biol 14:906-8. 2012..The second reports that membrane crowding, driven by protein-protein interactions, can also drive membrane bending, even in the absence of any protein insertion into the bilayer...
- Limited transferrin receptor clustering allows rapid diffusion of canine parvovirus into clathrin endocytic structuresDavid K Cureton
Immune Disease Institute and Program in Cellular and Molecular Medicine at Children s Hospital Boston, Boston, Massachusetts, USA
J Virol 86:5330-40. 2012....
- Inhibition of JCPyV infection mediated by targeted viral genome editing using CRISPR/Cas9Yi Ying Chou
Department of Cell Biology, Harvard Medical School, and Program in Cellular and Molecular Medicine, Boston Children s Hospital, Boston, MA 02115, USA
Sci Rep 6:36921. 2016..We found significant inhibition of virus replication and viral protein expression in cells recipient of Cas9 together with JCPyV-specific single-guide RNA delivered prior to or after JCPyV infection...
- Asymmetric formation of coated pits on dorsal and ventral surfaces at the leading edges of motile cells and on protrusions of immobile cellsComert Kural
Department of Cell Biology, Harvard Medical School, and Program in Cellular and Molecular Medicine, Boston Children s Hospital, Boston, MA 02115 Department of Physics, The Ohio State University, Columbus, OH 43210
Mol Biol Cell 26:2044-53. 2015..These observations support a previously proposed model that invokes net membrane deposition at the leading edge due to an imbalance between the endocytic and exocytic membrane flow at the front of a migrating cell. ..
- Live-cell imaging of clathrin coatsComert Kural
Department of Cell Biology, Harvard Medical School, and Immune Disease Institute and Program in Cellular and Molecular Medicine at Children s Hospital, Boston, Massachusetts, USA
Methods Enzymol 505:59-80. 2012....
- Cisternal organization of the endoplasmic reticulum during mitosisLei Lu
Department of Cell Biology and Immune Disease Institute, Harvard Medical School, Boston, MA 02115, USA
Mol Biol Cell 20:3471-80. 2009..In contrast, during interphase, the ER displays the familiar reticular network of convolved cisternae linked to tubules...
- Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustionYu Hu
Program in Cellular and Molecular Medicine, Boston Children s Hospital, Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115
J Exp Med 213:2759-2772. 2016..Our results demonstrate that scramblase restricts TCR responses to avoid overactivation, ensuring a well-balanced immune response in chronic infectious disease...
- Dynamin recruitment and membrane scission at the neck of a clathrin-coated pitEmanuele Cocucci
Department of Cell Biology, Harvard Medical School, and Cellular and Molecular Medicine Program, Boston Children s Hospital, Boston, MA 02115 Department of Pediatrics, Harvard Medical School, Boston, MA 02115
Mol Biol Cell 25:3595-609. 2014..We discuss how these results restrict models for the mechanism of dynamin-mediated membrane scission. ..
- Key interactions for clathrin coat stabilityTill Böcking
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA Centre for Vascular Research, University of New South Wales, Sydney NSW 2052 Australia Electronic address
Structure 22:819-29. 2014..Coats destabilized by these mutations require fewer Hsc70 molecules to initiate disassembly, as predicted by a model in which Hsc70 traps conformational distortions during the auxilin- and Hsc70:ATP-mediated uncoating reaction. ..
- Synergy between the ESCRT-III complex and Deltex defines a ligand-independent Notch signalKazuya Hori
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
J Cell Biol 195:1005-15. 2011..This activation mode of the receptor emphasizes the complexity of Notch signal modulation in a cell and has significant implications for both development and disease...
- Tracking molecular particles in live cells using fuzzy rule-based systemShan Jiang
HCNR Center for Bioinformatics, Harvard Medical School and Brigham and Women s Hospital, Boston, Massachusetts 02215, USA
Cytometry A 71:576-84. 2007..Results show that this method can accurately track most of the particles in the high volume images...
- Lethal skeletal dysplasia in mice and humans lacking the golgin GMAP-210Patrick Smits
Orthopedic Research Laboratories, Department of Orthopedic Surgery, Children s Hospital, Boston, MA 02115, USA
N Engl J Med 362:206-16. 2010..Establishing the genetic basis of phenotypes such as skeletal dysplasia in model organisms can provide insights into biologic processes and their role in human disease...
- T-cell engagement of dendritic cells rapidly rearranges MHC class II transportMarianne Boes
Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
Nature 418:983-8. 2002..We propose that such tubulation serves to facilitate the ensuing T-cell response...
- The small G-protein Arf6GTP recruits the AP-2 adaptor complex to membranesOlivia Paleotti
Institut de Pharmacologie Moleculaire et Cellulaire, CNRS Unité Mixte de Recherche 6097, 660 route des Lucioles, 06560 Valbonne Sophia Antipolis, France
J Biol Chem 280:21661-6. 2005..These findings strongly suggest that Arf6 plays a major role in clathrin-mediated endocytosis by directly controlling the assembly of the AP-2/clathrin coat...
- Detection of molecular particles in live cells via machine learningShan Jiang
Department of Mathematical Sciences, Tsinghua University, Beijing, 100084, People s Republic of China
Cytometry A 71:563-75. 2007..In order to get the accurate boundaries of these particles, several post-processing methods are applied and signal-to-noise ratio analysis is also performed to rule out the weak spots...
- Inhibition of dynamin completely blocks compensatory synaptic vesicle endocytosisA Jamila Newton
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
Proc Natl Acad Sci U S A 103:17955-60. 2006..In sum, our data indicate that dynamin is essential for all forms of compensatory synaptic vesicle endocytosis including any kiss-and-run events...
- An emergency response team for membrane repairPaul L McNeil
Department of Anatomy and Cellular Biology, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30921, USA
Nat Rev Mol Cell Biol 6:499-505. 2005....