Genomes and Genes
Affiliation: Harvard University
- Integrated genome analysis suggests that most conserved non-coding sequences are regulatory factor binding sitesMartin Hemberg
Department of Ophthalmology, Children s Hospital Boston, Harvard Medical School, Boston, MA 02215, USA
Nucleic Acids Res 40:7858-69. 2012..Extending these findings across multiple cell types and tissues, we propose that most conserved non-coding genomic DNA in vertebrate genomes corresponds to promoter-distal regulatory elements...
- Quantification of mRNA in single cells and modelling of RT-qPCR induced noiseMartin Bengtsson
Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, The Churchill Hospital, Oxford, OX3 7LJ, UK
BMC Mol Biol 9:63. 2008..Quantitative reverse transcription PCR (RT-qPCR) is the most accessible method which provides sufficiently accurate measurements of mRNA in single cells...
- Widespread transcription at neuronal activity-regulated enhancersTae Kyung Kim
Department of Neurobiology, Harvard Medical School, 220 Longwood Avenue, Boston, Massachusetts 02115, USA
Nature 465:182-7. 2010..These findings reveal that a widespread mechanism of enhancer activation involves RNAPII binding and eRNA synthesis...
- Disruption of DNA-methylation-dependent long gene repression in Rett syndromeHarrison W Gabel
Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 522:89-93. 2015..These findings suggest that mutations in MeCP2 may cause neurological dysfunction by specifically disrupting long gene expression in the brain. ..
- Genome-wide activity-dependent MeCP2 phosphorylation regulates nervous system development and functionSonia Cohen
Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
Neuron 72:72-85. 2011..We propose that RTT results in part from a loss of this experience-dependent chromatin remodeling...
- MEF2D drives photoreceptor development through a genome-wide competition for tissue-specific enhancersMilena M Andzelm
Department of Neurobiology, Harvard Medical School, 220 Longwood Avenue, Boston MA 02115, USA
Neuron 86:247-63. 2015..These findings demonstrate that broadly expressed TFs acquire specific functions through competitive recruitment to enhancers by tissue-specific TFs and through selective activation of these enhancers to regulate tissue-specific genes. ..
- Bhlhb5 and Prdm8 form a repressor complex involved in neuronal circuit assemblySarah E Ross
Department of Neurobiology, Harvard Medical School, 220 Longwood Avenue, Boston, MA 02115, USA
Neuron 73:292-303. 2012..These findings suggest that Prdm8 is an obligate partner of Bhlhb5, forming a repressor complex that directs neural circuit assembly in part through the precise regulation of Cadherin-11...
- Conservation of transcription factor binding events predicts gene expression across speciesMartin Hemberg
Children s Hospital Boston, Program in Biophysics and Program in Neuroscience, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
Nucleic Acids Res 39:7092-102. 2011..These results link conservation of structural elements (TFBEs) to conservation of function (gene expression) and suggest a higher degree of functional conservation than implied by previous studies...
- Genome-wide identification and characterization of functional neuronal activity-dependent enhancersAthar N Malik
1 Department of Neurobiology, Harvard Medical School, Boston, Massachusetts, USA 2 MD PhD Program, Harvard Medical School, Boston, Massachusetts, USA 3 Division of Health Sciences and Technology, Harvard Medical School and Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Nat Neurosci 17:1330-9. 2014....
- Quantitative profiling of peptides from RNAs classified as noncodingSudhakaran Prabakaran
1 Proteomics Center, Boston Children s Hospital, Boston, Massachusetts 02115, USA 2 Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Commun 5:5429. 2014..These observations highlight a potentially large and complex set of biologically regulated translational events from transcripts formerly thought to lack coding potential...
- Genome-wide analysis of MEF2 transcriptional program reveals synaptic target genes and neuronal activity-dependent polyadenylation site selectionSteven W Flavell
FM Kirby Neurobiology Center, Children s Hospital, and Department of Neurology and Neurobiology, Harvard Medical School, Boston, MA 02115, USA
Neuron 60:1022-38. 2008..Taken together, these analyses suggest that the ubiquitously expressed transcription factor MEF2 regulates an intricate transcriptional program in neurons that controls synapse development...