Nobuharu Fujii

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Role of AMP-activated protein kinase in exercise capacity, whole body glucose homeostasis, and glucose transport in skeletal muscle -insight from analysis of a transgenic mouse model-
    Nobuharu Fujii
    Research Division, Joslin Diabetes Center, and the Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, One Joslin Place, Boston, MA 02215, USA
    Diabetes Res Clin Pract 77:S92-8. 2007
  2. ncbi request reprint Muscle-specific overexpression of wild type and R225Q mutant AMP-activated protein kinase gamma3-subunit differentially regulates glycogen accumulation
    Haiyan Yu
    Joslin Diabetes Center, Boston, MA 02215, USA
    Am J Physiol Endocrinol Metab 291:E557-65. 2006
  3. ncbi request reprint AMP-activated protein kinase alpha2 activity is not essential for contraction- and hyperosmolarity-induced glucose transport in skeletal muscle
    Nobuharu Fujii
    Research Division, Joslin Diabetes Center, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 280:39033-41. 2005
  4. pmc Dissociation of AMP-activated protein kinase and p38 mitogen-activated protein kinase signaling in skeletal muscle
    Richard C Ho
    Research Division, Joslin Diabetes Center, Boston, MA 02215, USA
    Biochem Biophys Res Commun 362:354-9. 2007
  5. pmc Sucrose nonfermenting AMPK-related kinase (SNARK) mediates contraction-stimulated glucose transport in mouse skeletal muscle
    Ho Jin Koh
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 107:15541-6. 2010
  6. ncbi request reprint Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle
    Henning F Kramer
    Section Head, Metabolism, Joslin Diabetes Center, One Joslin Pl, Boston, MA 02215, USA
    Diabetes 55:2067-76. 2006
  7. ncbi request reprint Calmodulin-binding domain of AS160 regulates contraction- but not insulin-stimulated glucose uptake in skeletal muscle
    Henning F Kramer
    Department of Metabolism, Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA
    Diabetes 56:2854-62. 2007
  8. ncbi request reprint Regulation of dishevelled and beta-catenin in rat skeletal muscle: an alternative exercise-induced GSK-3beta signaling pathway
    William G Aschenbach
    Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA
    Am J Physiol Endocrinol Metab 291:E152-8. 2006
  9. pmc CaMKII regulates contraction- but not insulin-induced glucose uptake in mouse skeletal muscle
    Carol A Witczak
    Joslin Diabetes Center, Research Division, Department of Medicine, Brigham and Women sHospital, and Harvard Medical School, Boston, MA, 02215, USA
    Am J Physiol Endocrinol Metab 298:E1150-60. 2010
  10. pmc TBC1D1 regulates insulin- and contraction-induced glucose transport in mouse skeletal muscle
    Ding An
    Research Division, Joslin Diabetes Center, Boston, Massachusetts, USA
    Diabetes 59:1358-65. 2010

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Role of AMP-activated protein kinase in exercise capacity, whole body glucose homeostasis, and glucose transport in skeletal muscle -insight from analysis of a transgenic mouse model-
    Nobuharu Fujii
    Research Division, Joslin Diabetes Center, and the Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, One Joslin Place, Boston, MA 02215, USA
    Diabetes Res Clin Pract 77:S92-8. 2007
    ..However, contraction- or hyperosmolarity-induced glucose transport was nearly normal. These results suggest that AMPK alpha2 activation is essential for some, but not all insulin-independent glucose transport...
  2. ncbi request reprint Muscle-specific overexpression of wild type and R225Q mutant AMP-activated protein kinase gamma3-subunit differentially regulates glycogen accumulation
    Haiyan Yu
    Joslin Diabetes Center, Boston, MA 02215, USA
    Am J Physiol Endocrinol Metab 291:E557-65. 2006
    ..Furthermore, mutation of the gamma3-subunit is associated with decreases in AMPKalpha2 isoform-specific activity and impairment in AICAR- and phenformin-stimulated skeletal muscle glucose uptake...
  3. ncbi request reprint AMP-activated protein kinase alpha2 activity is not essential for contraction- and hyperosmolarity-induced glucose transport in skeletal muscle
    Nobuharu Fujii
    Research Division, Joslin Diabetes Center, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 280:39033-41. 2005
    ..Muscle contraction- and hyperosmolarity-induced glucose transport may be regulated by a redundant mechanism in which AMPK alpha2 is one of multiple signaling pathways...
  4. pmc Dissociation of AMP-activated protein kinase and p38 mitogen-activated protein kinase signaling in skeletal muscle
    Richard C Ho
    Research Division, Joslin Diabetes Center, Boston, MA 02215, USA
    Biochem Biophys Res Commun 362:354-9. 2007
    ..Furthermore, AMPKalpha2i TG mice lacking contraction-stimulated AMPK activity have normal p38 MAPK phosphorylation. These results suggest that p38 MAPK is not a downstream component of AMPK-mediated signaling in skeletal muscle...
  5. pmc Sucrose nonfermenting AMPK-related kinase (SNARK) mediates contraction-stimulated glucose transport in mouse skeletal muscle
    Ho Jin Koh
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 107:15541-6. 2010
    ..SNARK is activated by muscle contraction and is a unique mediator of contraction-stimulated glucose transport in skeletal muscle...
  6. ncbi request reprint Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle
    Henning F Kramer
    Section Head, Metabolism, Joslin Diabetes Center, One Joslin Pl, Boston, MA 02215, USA
    Diabetes 55:2067-76. 2006
    ..While Akt and AMPK alpha2 activities are essential for AS160 phosphorylation by insulin and AICAR, respectively, neither kinase is indispensable for the entire effects of contraction on AS160 phosphorylation...
  7. ncbi request reprint Calmodulin-binding domain of AS160 regulates contraction- but not insulin-stimulated glucose uptake in skeletal muscle
    Henning F Kramer
    Department of Metabolism, Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA
    Diabetes 56:2854-62. 2007
    ..This raises the possibility that contraction-stimulated increases in Ca(2+)/calmodulin could also modulate AS160 function...
  8. ncbi request reprint Regulation of dishevelled and beta-catenin in rat skeletal muscle: an alternative exercise-induced GSK-3beta signaling pathway
    William G Aschenbach
    Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA
    Am J Physiol Endocrinol Metab 291:E152-8. 2006
    ..In conclusion, exercise in vivo, but not insulin, increases the association between Dvl and GSK-3beta in skeletal muscle, an event paralleled by beta-catenin dephosphorylation...
  9. pmc CaMKII regulates contraction- but not insulin-induced glucose uptake in mouse skeletal muscle
    Carol A Witczak
    Joslin Diabetes Center, Research Division, Department of Medicine, Brigham and Women sHospital, and Harvard Medical School, Boston, MA, 02215, USA
    Am J Physiol Endocrinol Metab 298:E1150-60. 2010
    ..These results demonstrate that CaMKII does not regulate insulin-stimulated glucose uptake in skeletal muscle. However, CaMKII plays a critical role in the regulation of contraction-induced glucose uptake in mouse skeletal muscle...
  10. pmc TBC1D1 regulates insulin- and contraction-induced glucose transport in mouse skeletal muscle
    Ding An
    Research Division, Joslin Diabetes Center, Boston, Massachusetts, USA
    Diabetes 59:1358-65. 2010
    ..Genetic linkage analyses show a TBC1D1 R125W missense variant confers risk for severe obesity in humans. The objective of this study was to determine whether TBC1D1 regulates glucose transport in skeletal muscle...
  11. pmc Ablation of LKB1 in the heart leads to energy deprivation and impaired cardiac function
    Niels Jessen
    Joslin Diabetes Center, Boston, MA 02215, USA
    Biochim Biophys Acta 1802:593-600. 2010
    ..Ablation of LKB1 leads to a decreased cardiac efficiency despite normal mitochondrial oxidative metabolism...
  12. pmc Ablation of AMP-activated protein kinase alpha2 activity exacerbates insulin resistance induced by high-fat feeding of mice
    Nobuharu Fujii
    Department of Medicine, Research Division, Joslin Diabetes Center, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Diabetes 57:2958-66. 2008
    ..We determined whether muscle AMP-activated protein kinase (AMPK) has a role in the development of insulin resistance...
  13. ncbi request reprint Functional role of AMP-activated protein kinase in the heart during exercise
    Nicolas Musi
    Research Division, Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA
    FEBS Lett 579:2045-50. 2005
    ..We found that inactivation of AMPK did not result in abnormal ATP and glycogen consumption during exercise, cardiac function assessed by heart rhythm telemetry and stress echocardiography, or in maximal exercise capacity...
  14. ncbi request reprint Skeletal muscle adaptation to exercise training: AMP-activated protein kinase mediates muscle fiber type shift
    Katja S C Röckl
    Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital, and Harvard Medical School, Boston, Massachusetts 02215, USA
    Diabetes 56:2062-9. 2007
    ..AMPK is not necessary for exercise training-induced increases in mitochondrial markers, but it is essential for fiber type IIb to IIa/x transformation and increases in hexokinase II protein...
  15. pmc Skeletal muscle-selective knockout of LKB1 increases insulin sensitivity, improves glucose homeostasis, and decreases TRB3
    Ho Jin Koh
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Mol Cell Biol 26:8217-27. 2006
    ..LKB1-mediated TRB3 expression provides a novel link between LKB1 and Akt, critical kinases involved in both tumor genesis and cell metabolism...
  16. ncbi request reprint Overexpression or ablation of JNK in skeletal muscle has no effect on glycogen synthase activity
    Nobuharu Fujii
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Cell Physiol 287:C200-8. 2004
    ..JNK activation is unlikely to be the major mechanism by which contractile activity increases glycogen synthase activity in skeletal muscle...
  17. ncbi request reprint Ca2+/calmodulin-dependent protein kinase kinase-alpha regulates skeletal muscle glucose uptake independent of AMP-activated protein kinase and Akt activation
    Carol A Witczak
    Research Division, Joslin Diabetes Center, Boston, MA 02215, USA
    Diabetes 56:1403-9. 2007
    ..Collectively, these results implicate CaMKKalpha in the regulation of skeletal muscle glucose uptake independent of AMPK and Akt activation...
  18. pmc Myo1c regulates glucose uptake in mouse skeletal muscle
    Taro Toyoda
    Section on Integrative Physiology and Metabolism, the Joslin Diabetes Center, Boston, Massachusetts 02215, USA
    J Biol Chem 286:4133-40. 2011
    ..Myo1c is a novel mediator of both insulin-stimulated and contraction-stimulated glucose uptake in skeletal muscle...
  19. ncbi request reprint Role of Akt2 in contraction-stimulated cell signaling and glucose uptake in skeletal muscle
    Kei Sakamoto
    The Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Endocrinol Metab 291:E1031-7. 2006
    ....
  20. ncbi request reprint p38gamma MAPK regulation of glucose transporter expression and glucose uptake in L6 myotubes and mouse skeletal muscle
    Richard C Ho
    Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA
    Am J Physiol Regul Integr Comp Physiol 286:R342-9. 2004
    ..These findings are consistent with the hypothesis that activation of stress kinases such as p38 are negative regulators of stimulated glucose uptake in peripheral tissues...
  21. ncbi request reprint Regulation of glucose transport by the AMP-activated protein kinase
    Nobuharu Fujii
    Research Division, Joslin Diabetes Center, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02215, USA
    Proc Nutr Soc 63:205-10. 2004
    ..Thus, during the next several years considerably more research will be necessary in order to fully understand the role of AMPK in regulating glucose transport in skeletal muscle...
  22. ncbi request reprint cDNA cloning and functional characterization of a novel splice variant of c-Cbl-associated protein from mouse skeletal muscle
    Oscar Alcazar
    The Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02215, USA
    Biochem Biophys Res Commun 317:285-93. 2004
    ..These data demonstrate the existence of a novel CAP isoform expressed in skeletal muscle, and suggest the involvement of the CAP/Cbl pathway in the regulation of insulin-stimulated glucose uptake in L6 myotubes...
  23. ncbi request reprint Glucose metabolism and energy homeostasis in mouse hearts overexpressing dominant negative alpha2 subunit of AMP-activated protein kinase
    Yanqiu Xing
    Nuclear Magnetic Resonance Laboratory for Physiological Chemistry, Cardiovascular Division and Research Division, Joslin Diabetes Center, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:28372-7. 2003
    ..Thus, AMPK alpha2 is necessary for maintaining myocardial energy homeostasis during ischemia. It is likely that the functional role of AMPK in myocardial energy metabolism resides both in energy supply and utilization...
  24. ncbi request reprint AMP-activated protein kinase and the regulation of glucose transport
    Nobuharu Fujii
    Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Endocrinol Metab 291:E867-77. 2006
    ..The identification of AMPK as a novel glucose transport mediator in skeletal muscle is providing important insights for the treatment and prevention of type 2 diabetes...
  25. ncbi request reprint Cloning and characterization of mouse 5'-AMP-activated protein kinase gamma3 subunit
    Haiyan Yu
    Research Division, Joslin Diabetes Center, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02215, USA
    Am J Physiol Cell Physiol 286:C283-92. 2004
    ....
  26. ncbi request reprint Effect of AICAR treatment on glycogen metabolism in skeletal muscle
    William G Aschenbach
    Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Diabetes 51:567-73. 2002
    ..Rather, the increased glycogen is due to the well-known effects of AICAR to increase glucose uptake...
  27. ncbi request reprint Involvement of c-Jun N-terminal kinase in oxidative stress-mediated suppression of insulin gene expression
    Hideaki Kaneto
    Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Boston, Massachusetts 02215, USA
    J Biol Chem 277:30010-8. 2002
    ..In conclusion, activation of JNK is involved in the reduction of insulin gene expression by oxidative stress, and suppression of the JNK pathway protects beta-cells from oxidative stress...
  28. ncbi request reprint AS160 regulates insulin- and contraction-stimulated glucose uptake in mouse skeletal muscle
    Henning F Kramer
    Joslin Diabetes Center Research Division, Metabolism Section, Massachusetts 02215, USA
    J Biol Chem 281:31478-85. 2006
    ..Our findings directly implicate AS160 as a critical convergence factor for independent stimulators of skeletal muscle glucose uptake...
  29. ncbi request reprint Isolation of auxin efflux carrier cDNA from cucumber
    Takuya Hotta
    Graduate School of Life Science, Tohoku University
    Biol Sci Space 17:230-1. 2003
    ..CsPIN5, CsPIN6 and AtPIN2 fall within the same clade. Our phylogenetic analysis of PIN in cucumber and Arabidopsis indicates that cucumber may diversify CsPIN protein compared with AtPIN protein...
  30. pmc Oxidative stress stimulates skeletal muscle glucose uptake through a phosphatidylinositol 3-kinase-dependent pathway
    Yasuki Higaki
    Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan
    Am J Physiol Endocrinol Metab 294:E889-97. 2008
    ..These results demonstrate that acute exposure of muscle to ROS is a potent stimulator of skeletal muscle glucose uptake and that this occurs through a PI3K-dependent mechanism...
  31. ncbi request reprint Long-term AICAR administration and exercise prevents diabetes in ZDF rats
    Rasmus Pold
    Medical Research Laboratory and Medical Department M Endocrinology and Diabetes, Aarhus University Hospital, Aarhus, Denmark
    Diabetes 54:928-34. 2005
    ..Our results suggest that activation of AMPK may represent a therapeutic approach to improve insulin action and prevent a decrease in beta-cell function associated with type 2 diabetes...
  32. ncbi request reprint Auxin response, but not its polar transport, plays a role in hydrotropism of Arabidopsis roots
    Tomoko Kaneyasu
    Graduate School of Life Sciences, Tohoku University, Sendai, 980 8577, Japan
    J Exp Bot 58:1143-50. 2007
    ..These results suggest that auxin response, apart from its polar transport, plays a definite role in hydrotropic response, and will evoke a new concept for the auxin-mediated regulation of tropisms...
  33. ncbi request reprint Gravity-induced modification of auxin transport and distribution for peg formation in cucumber seedlings: possible roles for CS-AUX1 and CS-PIN1
    Motoshi Kamada
    Graduate School of Life Sciences, Tohoku University, Katahira, Aoba ku, 980 8577 Sendai, Japan
    Planta 218:15-26. 2003
    ..It is therefore possible that a balance in the activities of auxin influx and efflux carriers controls intracellular auxin concentration at the transition zone, which results in lateral placement of a peg in cucumber seedlings...
  34. ncbi request reprint Cardiac hypertrophy by hypertension and exercise training exhibits different gene expression of enzymes in energy metabolism
    Motoyuki Iemitsu
    Cardiovascular Division, Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Japan
    Hypertens Res 26:829-37. 2003
    ....