M R Freeman

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. doi request reprint Caveolin-1 and prostate cancer progression
    Michael R Freeman
    Children s Hospital Boston, Boston, MA, USA
    Adv Exp Med Biol 729:95-110. 2012
  2. pmc A metabolic perturbation by U0126 identifies a role for glutamine in resveratrol-induced cell death
    Michael R Freeman
    The Urological Diseases Research Center, Children s Hospital Boston, MA, USA
    Cancer Biol Ther 12:966-77. 2011
  3. pmc Skp2 expression is associated with high risk and elevated Ki67 expression in gastrointestinal stromal tumours
    Dolores Di Vizio
    Department of Functional and Biomorphological Science, University Federico II, Naples, Italy
    BMC Cancer 8:134. 2008
  4. ncbi request reprint HER2/HER3 heterodimers in prostate cancer: Whither HER1/EGFR?
    Michael R Freeman
    Urological Diseases Research Center, Children s Hospital Boston, Harvard Medical School, Enders Research Laboratories, Suite 1161, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    Cancer Cell 6:427-8. 2004
  5. doi request reprint The Rafts of the Medusa: cholesterol targeting in cancer therapy
    M R Freeman
    Urological Diseases Research Center, Children s Hospital Boston, Boston, MA 02115, USA
    Oncogene 29:3745-7. 2010
  6. pmc Transit of hormonal and EGF receptor-dependent signals through cholesterol-rich membranes
    Michael R Freeman
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, MA 02115, United States
    Steroids 72:210-7. 2007
  7. ncbi request reprint Membrane rafts as potential sites of nongenomic hormonal signaling in prostate cancer
    Michael R Freeman
    The Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, MA 02115, USA
    Trends Endocrinol Metab 16:273-9. 2005
  8. pmc A signaling network in phenylephrine-induced benign prostatic hyperplasia
    Jayoung Kim
    Departments of Surgery andBiological Chemistry and Molecular Pharmacology, Harvard Medical School, The Urological Diseases Research Center, Children s Hospital, Boston, Massachusetts 02115, USA
    Endocrinology 150:3576-83. 2009
  9. pmc Cholesterol and benign prostate disease
    Michael R Freeman
    The Urological Diseases Research Center, Children s Hospital Boston, Enders Research Laboratories, 300 Longwood Ave, Boston, MA 02115, USA
    Differentiation 82:244-52. 2011
  10. pmc Heparin-binding EGF-like growth factor is an autocrine growth factor for human urothelial cells and is synthesized by epithelial and smooth muscle cells in the human bladder
    M R Freeman
    Urology Research, Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Clin Invest 99:1028-36. 1997

Detail Information

Publications84

  1. doi request reprint Caveolin-1 and prostate cancer progression
    Michael R Freeman
    Children s Hospital Boston, Boston, MA, USA
    Adv Exp Med Biol 729:95-110. 2012
    ..This chapter summarizes the current state of our understanding of the cellular and physiologic mechanisms in which caveolin-1 participates in the evolution of prostate cancer cell phenotypes...
  2. pmc A metabolic perturbation by U0126 identifies a role for glutamine in resveratrol-induced cell death
    Michael R Freeman
    The Urological Diseases Research Center, Children s Hospital Boston, MA, USA
    Cancer Biol Ther 12:966-77. 2011
    ..Further work on resveratrol and metabolism in cancer is warranted to ascertain if the glutamine dependence has clinical implications...
  3. pmc Skp2 expression is associated with high risk and elevated Ki67 expression in gastrointestinal stromal tumours
    Dolores Di Vizio
    Department of Functional and Biomorphological Science, University Federico II, Naples, Italy
    BMC Cancer 8:134. 2008
    ..The aim of our study was to investigate the role of morphologic parameters and expression of cells cycle regulators as prognosticators in GIST...
  4. ncbi request reprint HER2/HER3 heterodimers in prostate cancer: Whither HER1/EGFR?
    Michael R Freeman
    Urological Diseases Research Center, Children s Hospital Boston, Harvard Medical School, Enders Research Laboratories, Suite 1161, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    Cancer Cell 6:427-8. 2004
    ..Surprisingly, the EGFR is peripheral to this signaling mechanism. These results have implications for the design of targeted therapy for hormone-refractory prostate cancer...
  5. doi request reprint The Rafts of the Medusa: cholesterol targeting in cancer therapy
    M R Freeman
    Urological Diseases Research Center, Children s Hospital Boston, Boston, MA 02115, USA
    Oncogene 29:3745-7. 2010
    ..Because the pathways considered in their study are used by other types of tumor cells, one implication of this report is that cholesterol-targeting approaches may be applicable to other malignancies...
  6. pmc Transit of hormonal and EGF receptor-dependent signals through cholesterol-rich membranes
    Michael R Freeman
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, MA 02115, United States
    Steroids 72:210-7. 2007
    ..Understanding the manner in which these pathways converge within cholesterol-rich membranes may present new avenues for therapeutic intervention in hormone-dependent cancers...
  7. ncbi request reprint Membrane rafts as potential sites of nongenomic hormonal signaling in prostate cancer
    Michael R Freeman
    The Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, MA 02115, USA
    Trends Endocrinol Metab 16:273-9. 2005
    ..We discuss the hypothesis that the cholesterol-rich lipid-raft compartment(s) of cancer-cell membranes might provide privileged sites for nongenomic signals mediated by the AR...
  8. pmc A signaling network in phenylephrine-induced benign prostatic hyperplasia
    Jayoung Kim
    Departments of Surgery andBiological Chemistry and Molecular Pharmacology, Harvard Medical School, The Urological Diseases Research Center, Children s Hospital, Boston, Massachusetts 02115, USA
    Endocrinology 150:3576-83. 2009
    ..This is the first attempt to develop a comprehensive molecular network for histological BPH induced by adrenergic activation. The study also implicated clusterin as a novel biochemical target for therapy...
  9. pmc Cholesterol and benign prostate disease
    Michael R Freeman
    The Urological Diseases Research Center, Children s Hospital Boston, Enders Research Laboratories, 300 Longwood Ave, Boston, MA 02115, USA
    Differentiation 82:244-52. 2011
    ..Here we review the evidence that cholesterol metabolism plays a role in the incidence of benign prostate disease and we highlight possible therapeutic approaches based on this concept...
  10. pmc Heparin-binding EGF-like growth factor is an autocrine growth factor for human urothelial cells and is synthesized by epithelial and smooth muscle cells in the human bladder
    M R Freeman
    Urology Research, Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Clin Invest 99:1028-36. 1997
    ....
  11. pmc Heparin-binding EGF-like growth factor is up-regulated in the obstructed kidney in a cell- and region-specific manner and acts to inhibit apoptosis
    H T Nguyen
    Urologic Laboratory, Department of Urology, Children s Hospital, Boston, MA 02115, USA
    Am J Pathol 156:889-98. 2000
    ..These results suggest a potential mechanism by which the kidney protects itself from apoptosis triggered by urinary tract obstruction...
  12. ncbi request reprint Cyclic stretch activates p38 SAPK2-, ErbB2-, and AT1-dependent signaling in bladder smooth muscle cells
    H T Nguyen
    The Urologic Laboratory, Department of Urology, Children s Hospital, and Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    Am J Physiol Cell Physiol 279:C1155-67. 2000
    ..Further, we show that the Erk-MAPK pathway, which in most systems is linked to receptor-dependent cell growth responses, is not involved in progression to DNA synthesis or in the response of the HB-EGF gene to mechanical forces...
  13. ncbi request reprint Heparin-binding epidermal growth factor-like growth factor is an autocrine mediator of human prostate stromal cell growth in vitro
    J L Duque
    Urologic Laboratory, Children s Hospital, Division of Urologic Surgery, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    J Urol 165:284-8. 2001
    ..We assess the potential role of this growth factor as a stromal cell regulator...
  14. ncbi request reprint Amphiregulin is coordinately expressed with heparin-binding epidermal growth factor-like growth factor in the interstitial smooth muscle of the human prostate
    R M Adam
    Department of Urology, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Endocrinology 140:5866-75. 1999
    ..These findings indicate that ARG and HB-EGF are likely to be key mediators of directional signaling between SMC and epithelial cells in the human prostate and appear to be coordinately regulated...
  15. ncbi request reprint Heparin-binding EGF-like growth factor in the human prostate: synthesis predominantly by interstitial and vascular smooth muscle cells and action as a carcinoma cell mitogen
    M R Freeman
    Department of Urology, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biochem 68:328-38. 1998
    ..Further, the observation that HB-EGF is similar to EGF in mitogenic potency for human prostate carcinoma cells suggests that it may be one of the hypothesized stromal mediators of prostate cancer growth...
  16. ncbi request reprint Post-transcriptional regulation of the androgen receptor by Mammalian target of rapamycin
    Bekir Cinar
    Departments of Urology and Surgery, Urological Diseases Research Center, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 65:2547-53. 2005
    ..These findings reveal a novel mechanism for regulation of AR by a classic growth factor system and indicate that a rapamycin-sensitive post-transcriptional pathway can attenuate or possibly bypass AR-mediated signaling...
  17. pmc Cholesterol targeting alters lipid raft composition and cell survival in prostate cancer cells and xenografts
    Liyan Zhuang
    The Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    J Clin Invest 115:959-68. 2005
    ..Our results implicate membrane cholesterol in Akt signaling in both normal and malignant cells and provide evidence that PCa cells can become dependent on a cholesterol-regulated Akt pathway for cell survival...
  18. ncbi request reprint Signaling through PI3K/Akt mediates stretch and PDGF-BB-dependent DNA synthesis in bladder smooth muscle cells
    Rosalyn M Adam
    The Urologic Laboratory, Department of Urology, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts, USA
    J Urol 169:2388-93. 2003
    ..The effects of growth factor stimulation on pathway activation in bladder SMC were also investigated...
  19. ncbi request reprint CaT1 expression correlates with tumor grade in prostate cancer
    J B Peng
    Membrane Biology Program, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts, 02115, USA
    Biochem Biophys Res Commun 282:729-34. 2001
    ..These findings are the first to implicate a Ca(2+) channel in PCa progression and suggest that CaT1 may be a novel target for therapy...
  20. ncbi request reprint BAG-1 is a novel cytoplasmic binding partner of the membrane form of heparin-binding EGF-like growth factor: a unique role for proHB-EGF in cell survival regulation
    J Lin
    Urologic Laboratory, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:30127-32. 2001
    ....
  21. ncbi request reprint Peripheral blood T lymphocytes and lymphocytes infiltrating human cancers express vascular endothelial growth factor: a potential role for T cells in angiogenesis
    M R Freeman
    Urology Research Laboratory, Children s Hospital, Boston, Massachusetts 02115, USA
    Cancer Res 55:4140-5. 1995
    ..The finding that peripheral T cells and TIL in human tumors synthesize a factor known to be a specific mediator of neovascularization suggests a role for T lymphocytes as cellular effectors of angiogenesis...
  22. ncbi request reprint Cholesterol sensitivity of endogenous and myristoylated Akt
    Rosalyn M Adam
    Urological Diseases Research Center, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Cancer Res 67:6238-46. 2007
    ....
  23. ncbi request reprint Transactivation of ErbB1 and ErbB2 receptors by angiotensin II in normal human prostate stromal cells
    Jianqing Lin
    The Urologic Laboratory, Department of Urology, Children s Hospital, and the Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    Prostate 54:1-7. 2003
    ..Because the pressor peptide Ang II signals through GPCRs, we tested the possibility that Ang II could transactivate ErbB1/ErbB2 in human prostate stromal (hPS) cells...
  24. ncbi request reprint Cholesterol-rich lipid rafts mediate akt-regulated survival in prostate cancer cells
    Liyan Zhuang
    The Urologic Laboratory, Department of Urology, Harvard Medical School, Boston, MA 02115, USA
    Cancer Res 62:2227-31. 2002
    ..These results suggest that cholesterol present in membrane microdomains is a prominent mediator of survival in prostate cancer cells...
  25. ncbi request reprint Heparin-binding EGF-like growth factor expression increases selectively in bladder smooth muscle in response to lower urinary tract obstruction
    J G Borer
    Department of Urology, Children s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Lab Invest 79:1335-45. 1999
    ..These data support a physiologic role for HB-EGF as a mediator of hypertrophic bladder tissue growth...
  26. ncbi request reprint Mechanical strain delivers anti-apoptotic and proliferative signals to gingival fibroblasts
    T E Danciu
    Enders Research Laboratories, Rm 1150 2, Children s Hospital, 300 Longwood Avenue, Boston, MA 02115, USA
    J Dent Res 83:596-601. 2004
    ..Moreover, mechanical strain resulted in increased ERK kinase phosphorylation and PCNA expression. In conclusion, cyclic strain delivers anti-apoptotic and proliferative stimuli to hGF...
  27. pmc Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes
    Wei Yang
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Mol Cell Proteomics 9:54-70. 2010
    ..The PalmPISC approach and the large scale human S-acylated protein data set are expected to provide powerful tools to facilitate our understanding of the functions and mechanisms of protein S-acylation...
  28. ncbi request reprint Phosphoinositide 3-kinase-independent non-genomic signals transit from the androgen receptor to Akt1 in membrane raft microdomains
    Bekir Cinar
    Urological Diseases Research Center, Departments of Urology, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 282:29584-93. 2007
    ..Our results indicate that cholesterol-rich membrane microdomains play a role in transmitting non-genomic signals involving androgen and the Akt pathway in prostate cancer cells...
  29. ncbi request reprint Activation of the Erk mitogen-activated protein kinase pathway stimulates neuroendocrine differentiation in LNCaP cells independently of cell cycle withdrawal and STAT3 phosphorylation
    Jayoung Kim
    The Urologic Laboratory, Department of Urology, Children s Hospital, and the Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 62:1549-54. 2002
    ..They also demonstrate that withdrawal from the cell cycle is not a prerequisite for expression of NE characteristics by PCa...
  30. pmc Vascular endothelial growth factor-mediated autocrine stimulation of prostate tumor cells coincides with progression to a malignant phenotype
    S Soker
    Department of Urology, Children s Hospital, 300 Longwood Ave, Boston, MA 02115, USA
    Am J Pathol 159:651-9. 2001
    ..Our results suggest two roles for VEGF in tumor progression: a paracrine role as an angiogenic factor and a previously undescribed role as an autocrine mediator of tumor cell motility...
  31. pmc Oncosome formation in prostate cancer: association with a region of frequent chromosomal deletion in metastatic disease
    Dolores Di Vizio
    The Urological Diseases Research Center, Children s Hospital Boston, Boston, MA 02115, USA
    Cancer Res 69:5601-9. 2009
    ..They also show that DRF3 is a physiologically relevant protein that seems to regulate this process...
  32. ncbi request reprint Heparin-binding epidermal growth factor-like growth factor stimulates androgen-independent prostate tumor growth and antagonizes androgen receptor function
    Rosalyn M Adam
    The Urologic Laboratory, Department of Urology, Children s Hospital Boston and Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    Endocrinology 143:4599-608. 2002
    ..These findings also emphasize the potential role of pathways other than the AR axis in acquisition of androgen independence...
  33. ncbi request reprint Involvement of cholesterol-rich lipid rafts in interleukin-6-induced neuroendocrine differentiation of LNCaP prostate cancer cells
    Jayoung Kim
    The Urologic Laboratory, John F Enders Research Laboratories, Room 1161, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
    Endocrinology 145:613-9. 2004
    ..Our results also suggest a mechanistic role for membrane cholesterol in cell signaling events relevant to PCa progression...
  34. ncbi request reprint Tumor cell-associated neuropilin-1 and vascular endothelial growth factor expression as determinants of tumor growth in neuroblastoma
    Karen Marcus
    Department of Radiation Oncology, Children s Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Neuropathology 25:178-87. 2005
    ..Our studies indicate that VEGF and VEGF receptor expression in NBL tumor cells are associated with tumor growth and that angiogenic factors may serve as a biological marker together with already established MYCN amplification...
  35. pmc Heterogeneous nuclear ribonucleoprotein K is a novel regulator of androgen receptor translation
    Nishit K Mukhopadhyay
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, MA 02115, USA
    Cancer Res 69:2210-8. 2009
    ..HnRNP-K is the first protein identified that directly interacts with and regulates the AR translational apparatus...
  36. ncbi request reprint Caveolin-1 is required for the upregulation of fatty acid synthase (FASN), a tumor promoter, during prostate cancer progression
    Dolores Di Vizio
    Urological Diseases Research Center, Children s Hopital Boston, Harvard Medical School, Enders Research Laboratories, Boston, Massachusetts, USA
    Cancer Biol Ther 6:1263-8. 2007
    ..In addition, our results provide the first molecular genetic evidence that Cav-1 functions upstream of FASN during prostate cancer progression...
  37. doi request reprint Do the cholesterol-lowering properties of statins affect cancer risk?
    Keith R Solomon
    Department of Orthopaedic Surgery, Children s Hospital Boston, Boston, MA 02115, USA
    Trends Endocrinol Metab 19:113-21. 2008
    ..Our hypothesis provides a perspective from which mechanistic studies of cholesterol-lowering drugs and cancer, in addition to prospective trials in patients, might be designed...
  38. pmc An absence of stromal caveolin-1 is associated with advanced prostate cancer, metastatic disease and epithelial Akt activation
    Dolores Di Vizio
    Urological Diseases Research Center, Children s Hospital Boston and Department of Surgery, Harvard Medical School, Enders Research Laboratories, Boston, MA 02115, USA
    Cell Cycle 8:2420-4. 2009
    ..This provides important new clinical evidence for paracrine signaling between prostate cancer epithelial cells and the tumor stromal micro-environment, especially related to disease progression and metastasis...
  39. ncbi request reprint The zinc finger protein ras-responsive element binding protein-1 is a coregulator of the androgen receptor: implications for the role of the Ras pathway in enhancing androgenic signaling in prostate cancer
    Nishit K Mukhopadhyay
    Department of Urology Surgery, Children s Hospital Boston, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    Mol Endocrinol 21:2056-70. 2007
    ..Collectively, our data for the first time identify RREB-1 as a repressor of AR and further implicate the Ras/MAPK kinase pathway as a likely antagonist of the inhibitory effects of RREB-1 on androgenic signaling...
  40. pmc The phosphoinositide kinase PIKfyve mediates epidermal growth factor receptor trafficking to the nucleus
    Jayoung Kim
    The Urological Diseases Research Center, Children s Hospital Boston, MA, USA
    Cancer Res 67:9229-37. 2007
    ..These results identify a novel mediator of the EGFR transcription function and further suggest that nuclear EGFR and the lipid kinase PIKfyve may play a role in bladder oncogenesis...
  41. pmc Quantitative proteomics analysis reveals molecular networks regulated by epidermal growth factor receptor level in head and neck cancer
    Wei Yang
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    J Proteome Res 9:3073-82. 2010
    ..Further investigation of these molecular networks may not only enhance our understanding of the antitumor mechanisms of EGFR targeting but also improve patient selection and provide novel targets for better therapeutics...
  42. pmc The pro-apoptotic kinase Mst1 and its caspase cleavage products are direct inhibitors of Akt1
    Bekir Cinar
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, MA, USA
    EMBO J 26:4523-34. 2007
    ..These results position Mst1/2 within a novel branch of the phosphoinositide 3-kinase/Akt pathway and suggest an important role in cancer progression...
  43. doi request reprint Temporal variations of the postnatal rat urinary proteome as a reflection of systemic maturation
    Richard S Lee
    Department of Urology, Harvard Medical School, Urological Diseases Research Center, Children s Hospital Boston, Boston, MA 02115, USA
    Proteomics 8:1097-112. 2008
    ..Further characterization of the normal urinary proteome may provide the basis for identification of urinary biomarkers of diseases of the urinary tract...
  44. ncbi request reprint Cholesterol and cholesterol-rich membranes in prostate cancer: an update
    Dolores Di Vizio
    The Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Harvard Medical School, Boston, MA 02115, USA
    Tumori 94:633-9. 2008
    ..This hypothesis provides new avenues for mechanistic studies as well as therapeutic intervention...
  45. ncbi request reprint Caveolin-1 interacts with a lipid raft-associated population of fatty acid synthase
    Dolores Di Vizio
    The Urological Diseases Research Center and Department of Surgery, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell Cycle 7:2257-67. 2008
    ..These findings suggest that FASN and Cav-1 physically and functionally interact in PCa cells. They also imply that palmitoylation within this complex is involved in tumor growth and survival...
  46. pmc Ezetimibe is an inhibitor of tumor angiogenesis
    Keith R Solomon
    Dept of Orthopaedic Surgery, Harvard Medical School, Boston, MA 02115, USA
    Am J Pathol 174:1017-26. 2009
    ....
  47. doi request reprint Optimizing sample handling for urinary proteomics
    Richard S Lee
    Department of Urology, Urological Diseases Research Center, Harvard Medical School and Children s Hospital Boston, Boston, Massachusetts 02115, USA
    J Proteome Res 7:4022-30. 2008
    ....
  48. pmc Heparin-binding epidermal growth factor-like growth factor functionally antagonizes interstitial cystitis antiproliferative factor via mitogen-activated protein kinase pathway activation
    Jayoung Kim
    The Urological Diseases Research Center, Children s Hospital Boston, Boston, MA, USA
    BJU Int 103:541-6. 2009
    ....
  49. pmc Pharmacologic management of painful bladder syndrome/interstitial cystitis: a systematic review
    Jordan Dimitrakov
    Harvard Urological Diseases Research Center, Children s Hospital Boston, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    Arch Intern Med 167:1922-9. 2007
    ..This study aimed to evaluate the efficacy of pharmacologic approaches to PBS/IC, to quantify the effect size from randomized controlled trials, and to begin to inform a clinical consensus of treatment efficacy for PBS/IC...
  50. ncbi request reprint Calcium regulates the PI3K-Akt pathway in stretched osteoblasts
    Theodora E Danciu
    Department of Orthopaedic Surgery, John F Enders Research Laboratories, Room 1230, Children s Hospital Boston, 300 Longwood Ave, Boston, MA 02115, USA
    FEBS Lett 536:193-7. 2003
    ....
  51. ncbi request reprint Increasing the ranks of physician-scientists in urology through the promotion of fellowship training: the example of pediatric urology
    Chester J Koh
    Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts, USA
    J Urol 173:2110-1. 2005
  52. ncbi request reprint Growth and stretch response of human exstrophy bladder smooth muscle cells: molecular evidence of normal intrinsic function
    Anna Orsola
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    BJU Int 95:144-8. 2005
    ..To establish primary cultures of smooth muscle cells (SMC) from human exstrophic bladders (E-SMC), and determine their in vitro growth dynamics and responses to mechanical stretch...
  53. pmc Management of chronic prostatitis/chronic pelvic pain syndrome: an evidence-based approach
    Jordan D Dimitrakov
    Harvard Urological Diseases Research Center, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
    Urology 67:881-8. 2006
  54. ncbi request reprint Unraveling androgen receptor interactomes by an array-based method: discovery of proto-oncoprotein c-Rel as a negative regulator of androgen receptor
    Nishit K Mukhopadhyay
    Molecular Urology Laboratory, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Exp Cell Res 312:3782-95. 2006
    ..In summary, we have identified several new partners of AR by using the TF-TF array method and have provided the first evidence of a functional role for c-Rel in androgen-responsive human prostate cancer cells...
  55. ncbi request reprint Mechanical stretch is a highly selective regulator of gene expression in human bladder smooth muscle cells
    Rosalyn M Adam
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts 02115, USA
    Physiol Genomics 20:36-44. 2004
    ..Moreover, they suggest that mechanical and growth factor signals converge on common transcriptional regulators that include members of the AP-1 family...
  56. ncbi request reprint Cholesterol and prostate cancer
    Michael R Freeman
    The Urological Diseases Research Center, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    J Cell Biochem 91:54-69. 2004
    ....
  57. ncbi request reprint The phosphatidylinositol 3'-kinase pathway is a dominant growth factor-activated cell survival pathway in LNCaP human prostate carcinoma cells
    J Lin
    Department of Urology, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 59:2891-7. 1999
    ..These results also show that survival signals, as distinguished from mitogenic signals, can be evoked in PCa cells by ErbB1 ligands known to be synthesized within the human prostate...
  58. ncbi request reprint Inhibition of EGFR signaling abrogates smooth muscle proliferation resulting from sustained distension of the urinary bladder
    Carlos R Estrada
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, MA 02115, USA
    Lab Invest 86:1293-302. 2006
    ..Our results indicate that the EGFR pathway is a physiologically relevant signaling mechanism in hypertrophic bladder disease resulting from mechanical distension and may be amenable to pharmacologic intervention...
  59. ncbi request reprint A quantitative proteomic analysis of growth factor-induced compositional changes in lipid rafts of human smooth muscle cells
    Dawn L MacLellan
    Department of Urology, The Urological Diseases Research Center, Boston, MA, USA
    Proteomics 5:4733-42. 2005
    ..In addition, eight Rho family members were localized to rafts by ICAT analysis. Collectively, these observations suggest a role for lipid rafts in regulation of PDGF-stimulated changes in the cytoskeleton...
  60. ncbi request reprint Measurement of plasma levels of vascular endothelial growth factor in prostate cancer patients: relationship with clinical stage, Gleason score, prostate volume, and serum prostate-specific antigen
    José Luis Ferreira Duque
    Brigham and Women s Hospital, Children s Hospital, Harvard Medical School, Boston, MA, USA
    Clinics (Sao Paulo) 61:401-8. 2006
    ..This study focused on circulating levels of vascular endothelial growth factor in patients with prostate cancer compared to a normal population...
  61. ncbi request reprint JNK/SAPK mediates doxorubicin-induced differentiation and apoptosis in MCF-7 breast cancer cells
    Jayoung Kim
    Urologic Laboratory, Department of Urology, Children s Hospital Boston, MA 02115, USA
    Breast Cancer Res Treat 79:321-8. 2003
    ..These findings support the possibility that JNK/SAPK pathway activation may be a means of therapeutic intervention in breast cancer...
  62. ncbi request reprint An oxidative stress mechanism mediates chelerythrine-induced heparin-binding EGF-like growth factor ectodomain shedding
    Jayoung Kim
    The Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Massachusetts 02115, USA
    J Cell Biochem 94:39-49. 2005
    ..However, JNK signaling was involved in chelerythrine-stimulated apoptosis. Our results suggest that HB-EGF shedding induced by chelerythrine is mediated predominantly via the production of reactive oxygen species...
  63. pmc p53 mediates interstitial cystitis antiproliferative factor (APF)-induced growth inhibition of human urothelial cells
    Jayoung Kim
    The Urological Diseases Research Center, Children s Hospital Boston, Boston, MA 02115, USA
    FEBS Lett 581:3795-9. 2007
    ..These are the first findings implicating the network of p53 target genes in urothelial defects associated with interstitial cystitis...
  64. doi request reprint Cholesterol-regulated stress fiber formation
    Maosong Qi
    Department of Orthopaedic Surgery, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    J Cell Biochem 106:1031-40. 2009
    ..These results demonstrate that cholesterol is a critical regulator of membrane-cytoskeletal dynamics and suggest that altered cholesterol concentrations may result in dramatic changes in cellular responses mediated by the cytoskeleton...
  65. pmc The ubiquitin-specific protease USP2a prevents endocytosis-mediated EGFR degradation
    Z Liu
    Department of Medical Oncology and Center for Molecular Oncologic Pathology, Dana Farber Cancer Institute, Boston, MA 02215, USA
    Oncogene 32:1660-9. 2013
    ..Our findings suggest that regulation of deubiquitination could be exploited therapeutically in cancers overexpressing EGFR...
  66. ncbi request reprint Increased incidence of matrix metalloproteinases in urine of cancer patients
    M A Moses
    Laboratory for Surgical Research, Children s Hospital, Boston, Massachusetts 02115, USA
    Cancer Res 58:1395-9. 1998
    ..This is the first study to demonstrate that analysis of urinary MMPs may be useful in determining disease status in a variety of human cancers, both within and outside of the urinary tract...
  67. ncbi request reprint The role of cholesterol in prostate cancer
    Martin H Hager
    The Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Massachusetts 02115, USA
    Curr Opin Clin Nutr Metab Care 9:379-85. 2006
    ..This review seeks to provide an overview of established links between cholesterol and prostate cancer, with an emphasis on very recent scientific contributions supporting a role of cholesterol in prostate cancer etiology...
  68. doi request reprint Ezetimibe reduces enlarged prostate in an animal model of benign prostatic hyperplasia
    Kristine Pelton
    Department of Orthopaedic Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Urol 184:1555-9. 2010
    ..We report that the Bio 87.20 hamster strain (Bio Breeders, Watertown, Massachusetts) shows 5α-reductase-sensitive prostate enlargement and a decrease in circulating cholesterol reduces prostate size...
  69. ncbi request reprint Partial ureteral obstruction dysregulates the renal renin-angiotensin system in the fetal sheep kidney
    S Ayan
    Department of Urology, Children's Hospital, Boston, Massachusetts 02115, USA
    Urology 58:301-6. 2001
    ..RAS components and growth factors, particularly TGF-beta1, may be considered relevant targets in the prevention and treatment of congenital obstructive nephropathy...
  70. pmc Proteomics and opportunities for clinical translation in urological disease
    Ali R Vaezzadeh
    Department of Urology, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts, USA
    J Urol 182:835-43. 2009
    ..We present an overview of current proteomics technologies, a review of proteomics applications in urology and a perspective on the future of proteomics in clinical medicine...
  71. ncbi request reprint Calcium-selective ion channel, CaT1, is apically localized in gastrointestinal tract epithelia and is aberrantly expressed in human malignancies
    Liyan Zhuang
    The Urologic Laboratory, Department of Urology, Children s Hospital Boston, Massachusetts 02115, USA
    Lab Invest 82:1755-64. 2002
    ..Our findings indicate that CaT1 is likely to serve as a component of transcellular calcium transport mechanisms in many tissues and epithelial cancers...
  72. pmc Caveolin-1alpha and -1beta perform nonredundant roles in early vertebrate development
    Ping Ke Fang
    Urological Diseases Research Center, Department of Orthopaedic Surgery, Children s Hospital Boston, Harvard Medical School, Enders Research Laboratories, Suite 1161, 300 Longwood Ave, Boston, MA 02115, USA
    Am J Pathol 169:2209-22. 2006
    ..Cav-1alpha and -1beta morphants also exhibited disruption in the actin cytoskeleton. These results support important and previously unanticipated roles for the Caveolin-1 isoforms in vertebrate organogenesis...
  73. ncbi request reprint Caveolae are negative regulators of transforming growth factor-beta1 signaling in ureteral smooth muscle cells
    Maximilian Stehr
    Department of Urology Urological Diseases Research Center, Children s Hospital Boston, Massachusetts 02115, USA
    J Urol 172:2451-5. 2004
    ..In this study we determined whether growth inhibitory signaling by transforming growth factor-beta1 (TGF-beta1) is also regulated by caveolae in USMC...
  74. ncbi request reprint Trafficking of nuclear heparin-binding epidermal growth factor-like growth factor into an epidermal growth factor receptor-dependent autocrine loop in response to oxidative stress
    Jayoung Kim
    The Urological Diseases Research Center, Childrens Hospital, Boston, Massachusetts, USA
    Cancer Res 65:8242-9. 2005
    ..These findings show that the nucleus can serve as an intracellular reservoir for a secreted EGFR ligand and, thus, can contribute to an autocrine loop leading to cell proliferation and protection from apoptotic stimuli...
  75. pmc Marked disturbance of calcium homeostasis in mice with targeted disruption of the Trpv6 calcium channel gene
    Suzy D C Bianco
    Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    J Bone Miner Res 22:274-85. 2007
    ..Although our Trpv6 KO affects the closely adjacent EphB6 gene, the phenotype reported here is not related to EphB6 dysfunction...
  76. ncbi request reprint The decision to undergo DNA or protein synthesis is determined by the degree of mechanical deformation in human bladder muscle cells
    Anna Orsola
    Urologic Laboratory, Department of Urology, Children s Hospital Boston and Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    Urology 59:779-83. 2002
    ....
  77. ncbi request reprint Behavioral profiling of human transitional cell carcinoma ex vivo
    Carlos R Estrada
    Urological Diseases Research Center, Children s Hospital Boston, Massachusetts, USA
    Cancer Res 66:3078-86. 2006
    ....
  78. ncbi request reprint A nuclear form of the heparin-binding epidermal growth factor-like growth factor precursor is a feature of aggressive transitional cell carcinoma
    Rosalyn M Adam
    Departments of Urology and Surgery, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 63:484-90. 2003
    ..In addition, our findings suggest that nuclear proHB-EGF may play a role in disease progression in bladder cancer and possibly other cancers...
  79. doi request reprint Proteomic approaches to the analysis of multiprotein signaling complexes
    Wei Yang
    The Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, MA 02115, USA
    Proteomics 8:832-51. 2008
    ..Challenges associated with proteomic analysis of signaling complexes are also discussed...
  80. ncbi request reprint Acrolein induces cyclooxygenase-2 and prostaglandin production in human umbilical vein endothelial cells: roles of p38 MAP kinase
    Yong Seek Park
    Department of Biochemistry, Research Institute for Microbial Diseases, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Arterioscler Thromb Vasc Biol 27:1319-25. 2007
    ..Acrolein, a known toxin in tobacco smoke, might be involved in atherogenesis. This study examined the effect of acrolein on expression of cyclooxygenase-2 (COX-2) and prostaglandin (PG) production in endothelial cells...
  81. ncbi request reprint Platelet derived growth factor-BB is a potent mitogen for rat ureteral and human bladder smooth muscle cells: dependence on lipid rafts for cell signaling
    Maximilain Stehr
    J Urol 169:1165-70. 2003
    ..In this study we asked whether B, T and U smooth muscle cells (SMC) respond to several SMC growth factors and explored the role of caveolae/lipid raft membrane microdomains in signaling by one of these factors, PDGF-BB...
  82. pmc Identification of a negative regulatory cis-element in the enhancer core region of the prostate-specific antigen promoter: implications for intersection of androgen receptor and nuclear factor-kappaB signalling in prostate cancer cells
    Bekir Cinar
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Biochem J 379:421-31. 2004
    ..Collectively, these data indicate that cross-modulation between AR and NF-kappaB p65 transcription factors may occur by a novel mechanism involving binding to a common cis -DNA element...
  83. ncbi request reprint Statins and the risk of cancer
    Michael R Freeman
    JAMA 295:2720-1; author reply 2721-2. 2006
  84. ncbi request reprint Beta2 microglobulin: a surprising therapeutic target for prostate cancer and renal cell carcinoma
    Michael R Freeman
    J Urol 178:10-1. 2007