Genomes and Genes
Affiliation: Harvard University
- Functional genomic analysis of the Wnt-wingless signaling pathwayRamanuj DasGupta
Department of Genetics, Howard Hughes Medical Institute HHMI, Harvard Medical School, New Research Building, No 339, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
Science 308:826-33. 2005..High-throughput RNAi screens in cultured cells, followed by functional analyses in model organisms, prove to be a rapid means of identifying regulators of signaling pathways implicated in development and disease...
- Drosophila Wnt/Fz pathwaysRamanuj DasGupta
Department of Genetics, Harvard Medical School HHMI, Boston, MA 02115, USA
Sci STKE 2005:cm5. 2005..Upon Wnt/Wg stimulation, stabilized Arm translocates to the nucleus, where, together with LEF/TCF transcription factors, it activates downstream target genes that regulate numerous cell biological processes...
- Bili inhibits Wnt/beta-catenin signaling by regulating the recruitment of axin to LRP6Lorna S Kategaya
Department of Pharmacology, Howard Hughes Medical Institute HHMI, University of Washington School of Medicine and Institute for Stem Cell and Regenerative Medicine, Seattle, WA, USA
PLoS ONE 4:e6129. 2009..Insights into how the Frizzled/LRP6 receptor complex receives, transduces and terminates Wnt signals will enhance our understanding of the control of the Wnt/ss-catenin pathway...
- The Wingless morphogen gradient is established by the cooperative action of Frizzled and Heparan Sulfate Proteoglycan receptorsGyeong Hun Baeg
Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
Dev Biol 276:89-100. 2004..Taken together, our analysis illustrates how the coordinated activities of HSPGs and Fz/DFz2 shape the Wg morphogen gradient...
- PAPTi: a peptide aptamer interference toolkit for perturbation of protein-protein interaction networksJohannes T H Yeh
Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
Sci Rep 3:1156. 2013..Altogether, these studies demonstrate the power of the PAPTi approach to dissect specific PPI interactions within signaling networks...
- Using RNAi to catch Drosophila genes in a web of interactions: insights into cancer researchRamanuj DasGupta
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
Oncogene 23:8359-65. 2004..In this review, we discuss the promise of integrating genome-wide RNAi screens with proteomic approaches and small-molecule chemical genetic screens, towards improving our ability to understand and treat cancer...
- Notch modulates Wnt signalling by associating with Armadillo/beta-catenin and regulating its transcriptional activityPenny Hayward
Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK
Development 132:1819-30. 2005..The modulatory function of Notch described here, contributes to the establishment of a robust threshold for Wnt signalling which is likely to play important roles in both normal and pathological situations...
- Functional genomics reveals genes involved in protein secretion and Golgi organizationFrederic Bard
Cell and Developmental Biology Department, University of California San Diego, La Jolla, California 92093 0634, USA
Nature 439:604-7. 2006..Of the 20 new gene products characterized so far, several localize to the Golgi membranes and the endoplasmic reticulum...
- A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in DrosophilaRamanuj DasGupta
New York University School of Medicine Cancer Institute, Department of Pharmacology, First Avenue, New York, NY 10016, USA
Genome Biol 8:R203. 2007..Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes...
- Links between signal transduction, transcription and adhesion in epithelial bud developmentColin Jamora
Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, New York 10021, USA
Nature 422:317-22. 2003....
- A developmental conundrum: a stabilized form of beta-catenin lacking the transcriptional activation domain triggers features of hair cell fate in epidermal cells and epidermal cell fate in hair follicle cellsRamanuj DasGupta
Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
J Cell Biol 158:331-44. 2002..Finally, by varying the level of beta-catenin signaling during a cell fate program, the skin cell appears to be pliable, switching fates multiple times...