Yifan Cheng

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Reconstructing the endocytotic machinery
    Yifan Cheng
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California 94143, USA
    Methods Cell Biol 79:463-87. 2007
  2. ncbi request reprint Single particle reconstruction of the human apo-transferrin-transferrin receptor complex
    Yifan Cheng
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Struct Biol 152:204-10. 2005
  3. pmc Lipid-protein interactions in double-layered two-dimensional AQP0 crystals
    Tamir Gonen
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 438:633-8. 2005
  4. ncbi request reprint Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating
    Alexander Fotin
    Biophysics Graduate Program, Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:649-53. 2004
  5. ncbi request reprint Single particle reconstructions of the transferrin-transferrin receptor complex obtained with different specimen preparation techniques
    Yifan Cheng
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Mol Biol 355:1048-65. 2006
  6. pmc Reconfiguration of the proteasome during chaperone-mediated assembly
    SoYeon Park
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 497:512-6. 2013
  7. pmc Structure determination of clathrin coats to subnanometer resolution by single particle cryo-electron microscopy
    Alexander Fotin
    Biophysics Graduate Program, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Struct Biol 156:453-60. 2006
  8. pmc Cryo-electron tomography of clathrin-coated vesicles: structural implications for coat assembly
    Yifan Cheng
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Mol Biol 365:892-9. 2007
  9. pmc Evaluation of imaging plates as recording medium for images of negatively stained single particles and electron diffraction patterns of two-dimensional crystals
    Zongli Li
    Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Electron Microsc (Tokyo) 59:53-63. 2010
  10. ncbi request reprint Aquaporin-0 membrane junctions form upon proteolytic cleavage
    Tamir Gonen
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Mol Biol 342:1337-45. 2004

Collaborators

Detail Information

Publications23

  1. ncbi request reprint Reconstructing the endocytotic machinery
    Yifan Cheng
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California 94143, USA
    Methods Cell Biol 79:463-87. 2007
  2. ncbi request reprint Single particle reconstruction of the human apo-transferrin-transferrin receptor complex
    Yifan Cheng
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Struct Biol 152:204-10. 2005
    ..The density map does, however, support the model for the apoTf-TfR we previously proposed based on the dTf-TfR complex structure, and it suggests that receptor-bound apoTf prefers to adopt an open conformation...
  3. pmc Lipid-protein interactions in double-layered two-dimensional AQP0 crystals
    Tamir Gonen
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 438:633-8. 2005
    ..We were therefore able to build an atomic model for the lipid bilayer surrounding the AQP0 tetramers, and we describe lipid-protein interactions...
  4. ncbi request reprint Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating
    Alexander Fotin
    Biophysics Graduate Program, Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:649-53. 2004
    ..Auxilin binding produces a local change in heavy-chain contacts, creating a detectable global distortion of the clathrin coat. We propose a mechanism by which local destabilization of the lattice promotes general uncoating...
  5. ncbi request reprint Single particle reconstructions of the transferrin-transferrin receptor complex obtained with different specimen preparation techniques
    Yifan Cheng
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Mol Biol 355:1048-65. 2006
    ..Our results stress the need for critical evaluation of structures determined by single particle EM...
  6. pmc Reconfiguration of the proteasome during chaperone-mediated assembly
    SoYeon Park
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 497:512-6. 2013
    ..Thus, the Rpt-CP interface is reconfigured when the lid complex joins the nascent proteasome to form the mature holoenzyme...
  7. pmc Structure determination of clathrin coats to subnanometer resolution by single particle cryo-electron microscopy
    Alexander Fotin
    Biophysics Graduate Program, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Struct Biol 156:453-60. 2006
    ..These procedures should be generally applicable and may thus help determine the structures of other large protein assemblies to higher resolution by single particle cryo-electron microscopy...
  8. pmc Cryo-electron tomography of clathrin-coated vesicles: structural implications for coat assembly
    Yifan Cheng
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Mol Biol 365:892-9. 2007
    ..We suggest that the eccentric position of the vesicle reflects the polarity of assembly, from initiation of coat formation to membrane pinching...
  9. pmc Evaluation of imaging plates as recording medium for images of negatively stained single particles and electron diffraction patterns of two-dimensional crystals
    Zongli Li
    Department of Cell Biology, Howard Hughes Medical Institute, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Electron Microsc (Tokyo) 59:53-63. 2010
    ..IPs can thus be used to collect diffraction patterns, but CCD cameras are more convenient and remain the best option for recording electron diffraction patterns...
  10. ncbi request reprint Aquaporin-0 membrane junctions form upon proteolytic cleavage
    Tamir Gonen
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Mol Biol 342:1337-45. 2004
    ..This change of function of AQP0 from a water pore in the cortex to an adhesion molecule in the lens core constitutes another manifestation of the gene sharing concept originally proposed on the basis of the dual function of crystallins...
  11. ncbi request reprint Structure of the human transferrin receptor-transferrin complex
    Yifan Cheng
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 116:565-76. 2004
    ..When Tf binds receptor, its N-lobe moves by about 9 A with respect to its C-lobe. The structure of TfR-Tf complex helps account for known differences in the iron-release properties of free and receptor bound Tf...
  12. pmc Docking of the proteasomal ATPases' carboxyl termini in the 20S proteasome's alpha ring opens the gate for substrate entry
    David M Smith
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell 27:731-44. 2007
    ..Thus, the C termini of the proteasomal ATPases function like a "key in a lock" to induce gate opening and allow substrate entry...
  13. doi request reprint Collecting electron crystallographic data of two-dimensional protein crystals
    Richard K Hite
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA
    Methods Enzymol 481:251-82. 2010
    ..In the second part, we describe the procedures used to record high-resolution images and diffraction patterns...
  14. ncbi request reprint Activation of leukocyte beta2 integrins by conversion from bent to extended conformations
    Noritaka Nishida
    CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Immunity 25:583-94. 2006
    ..The results establish an intimate relationship between extension of beta(2) integrins and their activation in immune responses and leukocyte trafficking...
  15. pmc Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0
    Po Lin Chiu
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Struct Biol 192:163-73. 2015
    ....
  16. ncbi request reprint Molecular model for a complete clathrin lattice from electron cryomicroscopy
    Alexander Fotin
    Biophysics Graduate Program, Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:573-9. 2004
    ..These invariant local interactions appear to stabilize the lattice, allowing assembly and uncoating to be controlled by events at a few specific sites...
  17. ncbi request reprint Aquaporin-0 membrane junctions reveal the structure of a closed water pore
    Tamir Gonen
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 429:193-7. 2004
    ..The water pathway in AQP0 also contains an additional pore constriction, not seen in other known aquaporin structures, which may be responsible for pore gating...
  18. pmc A chimeric protein of simian immunodeficiency virus envelope glycoprotein gp140 and Escherichia coli aspartate transcarbamoylase
    Bing Chen
    Laboratory of Molecular Medicine, The Children s Hospital, Boston, Massachusetts 02115, USA
    J Virol 78:4508-16. 2004
    ..The production of the fusion proteins may enhance the possibilities for structure determination of the envelope glycoprotein either by electron cryomicroscopy or X-ray crystallography...
  19. pmc A fusion-intermediate state of HIV-1 gp41 targeted by broadly neutralizing antibodies
    Gary Frey
    Laboratory of Molecular Medicine, Children s Hospital, and Department of Pediatrics, Harvard Medical School, 320 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:3739-44. 2008
    ..These results help explain the rarity of 2F5- and 4E10-like antibody responses and suggest a strategy for eliciting them...
  20. pmc Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1
    Melanie D Ohi
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 28:871-85. 2007
    ..Comparison of the APC/C with another multiprotein E3 ligase, the SCF complex, uncovers remarkable structural similarities...
  21. ncbi request reprint ATP binding to PAN or the 26S ATPases causes association with the 20S proteasome, gate opening, and translocation of unfolded proteins
    David M Smith
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Mol Cell 20:687-98. 2005
    ....
  22. doi request reprint Polymorphic assemblies and crystalline arrays of lens tetraspanin MP20
    Tamir Gonen
    School of Biological Sciences, University of Auckland, Auckland, New Zealand
    J Mol Biol 376:380-92. 2008
    ..The various complexes formed by MP20 most likely reflect the many ways in which tetraspanins can interact with each other to allow formation of TEMs...
  23. pmc Mechanism of gate opening in the 20S proteasome by the proteasomal ATPases
    Julius Rabl
    Fachbereich Biologie, Chemie, Pharmazie, Freie Universitat Berlin, Takustrasse 3, 14195 Berlin, Germany
    Mol Cell 30:360-8. 2008
    ..This mechanism differs from that of PA26/28, which lacks the HbYX motif and does not cause alpha subunit rotation. These findings demonstrated how the ATPases' C termini function to facilitate substrate entry...