Seth L Alper

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Regulation of AE2-mediated Cl- transport by intracellular or by extracellular pH requires highly conserved amino acid residues of the AE2 NH2-terminal cytoplasmic domain
    A K Stewart
    Department of Medicine, Harvard Medical School, Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    J Gen Physiol 120:707-22. 2002
  2. ncbi request reprint The AE gene family of Cl/HCO3- exchangers
    Seth L Alper
    Department of Medicine, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    J Nephrol 15:S41-53. 2002
  3. ncbi request reprint Genetic diseases of acid-base transporters
    Seth L Alper
    Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Department of Medicine and Cell Biology, Harvard Medical School Boston, Massachusetts 02215, USA
    Annu Rev Physiol 64:899-923. 2002
  4. ncbi request reprint How pH regulates a pH regulator: a regulatory hot spot in the N-terminal cytoplasmic domain of the AE2 anion exchanger
    Seth L Alper
    Department of Medicine, Harvard Medical School, Boston, MA, USA
    Cell Biochem Biophys 36:123-36. 2002
  5. ncbi request reprint Regulation of Na+-independent Cl-/HCO3- exchangers by pH
    S L Alper
    Department of Medicine, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    JOP 2:171-5. 2001
  6. ncbi request reprint Expression of AE2 anion exchanger in mouse intestine
    S L Alper
    Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol 277:G321-32. 1999
  7. ncbi request reprint Alkaline-shifted pHo sensitivity of AE2c1-mediated anion exchange reveals novel regulatory determinants in the AE2 N-terminal cytoplasmic domain
    Christine E Kurschat
    Molecular and Vascular Medicine and Renal Units, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA
    J Biol Chem 281:1885-96. 2006
  8. doi request reprint Mouse strain-specific coding polymorphism in the Slc4a2/Ae2 gene encodes Ae2c2 variants differing in isoform-specific dominant negative activity
    Christine E Kurschat
    Molecular and Vascular Medicine and Renal Units, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA
    Exp Physiol 93:458-67. 2008
  9. ncbi request reprint Functional comparison of mouse slc26a6 anion exchanger with human SLC26A6 polypeptide variants: differences in anion selectivity, regulation, and electrogenicity
    Marina N Chernova
    Molecular and Vascular Medicine Unit and Renal Unit, Beth Israel Deaconess Medical Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 280:8564-80. 2005
  10. ncbi request reprint Zebrafish ae2.2 encodes a second slc4a2 anion exchanger
    Boris E Shmukler
    Molecular and Vascular Medicine and Renal Units, Beth Israel Deaconess Medical Center E RW763, 330 Brookline Ave, Boston, MA 02215, USA
    Am J Physiol Regul Integr Comp Physiol 294:R1081-91. 2008

Collaborators

Detail Information

Publications110 found, 100 shown here

  1. pmc Regulation of AE2-mediated Cl- transport by intracellular or by extracellular pH requires highly conserved amino acid residues of the AE2 NH2-terminal cytoplasmic domain
    A K Stewart
    Department of Medicine, Harvard Medical School, Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    J Gen Physiol 120:707-22. 2002
    ..Conserved, individual amino acid residues of the AE2 cytoplasmic domain contribute to independent regulation of anion exchange activity by pH(o) as well as pH(i)...
  2. ncbi request reprint The AE gene family of Cl/HCO3- exchangers
    Seth L Alper
    Department of Medicine, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    J Nephrol 15:S41-53. 2002
    ..Noncontiguous regions in the middle of the N-terminal cytoplasmic domain are of particular importance for acute regulation by several types of stimulus...
  3. ncbi request reprint Genetic diseases of acid-base transporters
    Seth L Alper
    Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Department of Medicine and Cell Biology, Harvard Medical School Boston, Massachusetts 02215, USA
    Annu Rev Physiol 64:899-923. 2002
    ..Study of acid-base transport disease-associated mutations should enhance our understanding of protein structure-function relationships and their impact on the physiology of cell, tissue, and organism...
  4. ncbi request reprint How pH regulates a pH regulator: a regulatory hot spot in the N-terminal cytoplasmic domain of the AE2 anion exchanger
    Seth L Alper
    Department of Medicine, Harvard Medical School, Boston, MA, USA
    Cell Biochem Biophys 36:123-36. 2002
    ..Noncontiguous regions in the middle of the N-terminal cytoplasmic domain are of particular importance for acute regulation by several types of stimulus...
  5. ncbi request reprint Regulation of Na+-independent Cl-/HCO3- exchangers by pH
    S L Alper
    Department of Medicine, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    JOP 2:171-5. 2001
    ..Little is known about the acute regulation of these modulators of intracellular and compartmental pH and volume...
  6. ncbi request reprint Expression of AE2 anion exchanger in mouse intestine
    S L Alper
    Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol 277:G321-32. 1999
    ..These localization studies will aid in the interpretation of anion exchanger function measured in epithelial sheets, isolated cells, and membrane vesicles...
  7. ncbi request reprint Alkaline-shifted pHo sensitivity of AE2c1-mediated anion exchange reveals novel regulatory determinants in the AE2 N-terminal cytoplasmic domain
    Christine E Kurschat
    Molecular and Vascular Medicine and Renal Units, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA
    J Biol Chem 281:1885-96. 2006
    ..Physiological coexpression of AE2c1 with other AE2 variant polypeptides in the same cell should extend the range over which changing pH(o) can regulate AE2 transport activity...
  8. doi request reprint Mouse strain-specific coding polymorphism in the Slc4a2/Ae2 gene encodes Ae2c2 variants differing in isoform-specific dominant negative activity
    Christine E Kurschat
    Molecular and Vascular Medicine and Renal Units, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA
    Exp Physiol 93:458-67. 2008
    ..Knowledge of strain-specific coding polymorphisms with potential functional consequences such as that of Ae2c2 should aid in interpretation of strain-specific phenotypes investigated in the mouse phenome project...
  9. ncbi request reprint Functional comparison of mouse slc26a6 anion exchanger with human SLC26A6 polypeptide variants: differences in anion selectivity, regulation, and electrogenicity
    Marina N Chernova
    Molecular and Vascular Medicine Unit and Renal Unit, Beth Israel Deaconess Medical Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 280:8564-80. 2005
    ..Thus, the orthologous mouse and human SLC26A6 proteins differ in anion selectivity, transport mechanism, and acute regulation, but both mediate electroneutral Cl(-)/HCO(3)(-) exchange...
  10. ncbi request reprint Zebrafish ae2.2 encodes a second slc4a2 anion exchanger
    Boris E Shmukler
    Molecular and Vascular Medicine and Renal Units, Beth Israel Deaconess Medical Center E RW763, 330 Brookline Ave, Boston, MA 02215, USA
    Am J Physiol Regul Integr Comp Physiol 294:R1081-91. 2008
    ..1 mRNA, or of both with nontoxic or minimally toxic levels of N-morpholino oligomers produced no grossly detectable morphological phenotype, and preserved normal structure of the head and the pronephric duct at 24 h postfertilization...
  11. pmc The GPA-dependent, spherostomatocytosis mutant AE1 E758K induces GPA-independent, endogenous cation transport in amphibian oocytes
    Andrew K Stewart
    Division of Nephrology, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    Am J Physiol Cell Physiol 298:C283-97. 2010
    ....
  12. pmc Species differences in Cl- affinity and in electrogenicity of SLC26A6-mediated oxalate/Cl- exchange correlate with the distinct human and mouse susceptibilities to nephrolithiasis
    Jeffrey S Clark
    Molecular and Vascular Medicine Unit and Renal Division, Beth Israel Deaconess Medical Center and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    J Physiol 586:1291-306. 2008
    ..SLC26A6 sequence variant(s) are candidate risk modifiers for nephrolithiasis...
  13. ncbi request reprint Deficient HCO3- transport in an AE1 mutant with normal Cl- transport can be rescued by carbonic anhydrase II presented on an adjacent AE1 protomer
    Neera K Dahl
    Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 278:44949-58. 2003
    ..The data strongly support the hypothesis that an intact transbilayer anion translocation pathway is completely contained within an AE1 monomer...
  14. pmc Functional characterization and modified rescue of novel AE1 mutation R730C associated with overhydrated cation leak stomatocytosis
    Andrew K Stewart
    Division of Nephrology, Beth Israel Deaconess Medical Center, 330 Brookline Ave, Boston, MA 02215, USA
    Am J Physiol Cell Physiol 300:C1034-46. 2011
    ..Selective rescue of acid pH(o)-stimulated sulfate uptake and conferral of acid pH(o)-stimulated Cl(-) influx, by AE1 R730E and MTSES-treated R730C, define residue R730 as critical to selectivity and regulation of anion transport by AE1...
  15. pmc SLC26 anion exchangers of guinea pig pancreatic duct: molecular cloning and functional characterization
    Andrew K Stewart
    Renal Division and Vascular Biology Center, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Am J Physiol Cell Physiol 301:C289-303. 2011
    ..This first molecular and functional assessment of recombinant SLC26 anion transporters from guinea pig pancreatic duct enhances our understanding of pancreatic HCO(3)(-) secretion in species that share a high HCO(3)(-) secretory output...
  16. pmc Regulated transport of sulfate and oxalate by SLC26A2/DTDST
    John F Heneghan
    Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Am J Physiol Cell Physiol 298:C1363-75. 2010
    ..Diastrophic dysplasia mutants R279W and A386V of hSLC26A2 exhibited similar reductions in uptake of both (35)SO(4)(2-) and [(14)C]oxalate. A386V surface abundance was reduced, but R279W surface abundance was at wild-type levels...
  17. ncbi request reprint Anion exchangers in flux: functional differences between human and mouse SLC26A6 polypeptides
    Seth L Alper
    Molecular and Vascular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Novartis Found Symp 273:107-19; discussion 119-25, 261-4. 2006
    ..Understanding of SLC26 transport mechanisms and pathophysiology will benefit from recognition of substantial differences in transport properties among orthologues...
  18. ncbi request reprint Expression of the polycystin-1 C-terminal cytoplasmic tail increases Cl channel activity in Xenopus oocytes
    Marina N Chernova
    Molecular and Vascular Medicine Unit and Renal Division, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA
    Kidney Int 68:632-41. 2005
    ..Conclusion. These data show that CD16.7-PKD1 (115-226) activates Cl(-) channels in the Xenopus oocyte plasma membrane in parallel with, but not secondary to, activation of Ca(2+)-permeable cation channels...
  19. pmc Putative re-entrant loop 1 of AE2 transmembrane domain has a major role in acute regulation of anion exchange by pH
    Andrew K Stewart
    Department of Medicine, Harvard Medical School, and Molecular and Vascular Medicine Unit, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    J Biol Chem 284:6126-39. 2009
    ....
  20. pmc Attenuated, flow-induced ATP release contributes to absence of flow-sensitive, purinergic Cai2+ signaling in human ADPKD cyst epithelial cells
    Chang Xu
    Molecular and Vascular Medicine and Renal Divs, Beth Israel Deaconess Medical Center, 330 Brookline Ave, E RW763, Boston, MA 02215, USA
    Am J Physiol Renal Physiol 296:F1464-76. 2009
    ....
  21. ncbi request reprint Structure-function relationships of AE2 regulation by Ca(i)(2+)-sensitive stimulators NH(4+) and hypertonicity
    Marina N Chernova
    Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Cell Physiol 284:C1235-46. 2003
    ..AE2 was insensitive to many other modifiers of Ca(2+) signaling. Unlike AE2 stimulation by NH(4)(+) and by hypertonicity, AE2 inhibition by calmidazolium required only AE2's COOH-terminal transmembrane domain...
  22. pmc Pendrin function and regulation in Xenopus oocytes
    Fabian R Reimold
    Renal Division and Molecular and Vascular Medicine Unit, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Cell Physiol Biochem 28:435-50. 2011
    ..Mutation of SLC26A2 serine residues conserved in PKCδ -sensitive SLC26 proteins but absent from pendrin failed to reduce PKCδ sensitivity of SLC26A2 (190)...
  23. pmc Calcium-activated potassium channels contribute to human coronary microvascular dysfunction after cardioplegic arrest
    Jun Feng
    Division of Cardiothoracic Surgery, BIDMC, LMOB 2A, 110 Francis Street, Boston, MA 02215, USA
    Circulation 118:S46-51. 2008
    ..We investigated the role of calcium-activated potassium (K(Ca)) channels in this dysfunction in the human coronary microvasculature...
  24. pmc Acute regulation of the SLC26A3 congenital chloride diarrhoea anion exchanger (DRA) expressed in Xenopus oocytes
    Marina N Chernova
    Molecular Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    J Physiol 549:3-19. 2003
    ..Thus, pharmacological modulation of DRA might be a useful adjunct treatment of cystic fibrosis...
  25. pmc Chemical crosslinking studies with the mouse Kcc1 K-Cl cotransporter
    Sabina Casula
    Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, USA
    Blood Cells Mol Dis 42:233-40. 2009
    ..These data add to evidence supporting the oligomeric state of KCC polypeptides...
  26. pmc Mouse Ae1 E699Q mediates SO42-i/anion-o exchange with [SO42-]i-dependent reversal of wild-type pHo sensitivity
    Marina N Chernova
    Molecular and Vascular Medicine Unit, Beth Israel Deaconess Med Ctr, 330 Brookline Ave, Boston, MA 02215, USA
    Am J Physiol Cell Physiol 295:C302-12. 2008
    ....
  27. pmc Substitution of transmembrane domain Cys residues alters pH(o)-sensitive anion transport by AE2/SLC4A2 anion exchanger
    Fabian R Reimold
    Renal Division and Molecular and Vascular Medicine Division, Beth Israel Deaconess Medical Center, 99 Brookline Avenue, RN 380F, Boston, MA 02215, USA
    Pflugers Arch 465:839-51. 2013
    ....
  28. ncbi request reprint Apparent receptor-mediated activation of Ca2+-dependent conductive Cl- transport by shark-derived polyaminosterols
    Marina N Chernova
    Molecular and Vascular Medicine Unit, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Am J Physiol Regul Integr Comp Physiol 289:R1644-58. 2005
    ....
  29. pmc Native and recombinant Slc26a3 (downregulated in adenoma, Dra) do not exhibit properties of 2Cl-/1HCO3- exchange
    Seth L Alper
    Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
    Am J Physiol Cell Physiol 300:C276-86. 2011
    ....
  30. ncbi request reprint Molecular physiology of SLC4 anion exchangers
    Seth L Alper
    Molecular and Vascular Medicine Unit, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Exp Physiol 91:153-61. 2006
    ..This growing body of structure-function data, together with increased structural information, will advance mechanistic understanding of SLC4 anion exchangers...
  31. pmc Human ADPKD primary cyst epithelial cells with a novel, single codon deletion in the PKD1 gene exhibit defective ciliary polycystin localization and loss of flow-induced Ca2+ signaling
    Chang Xu
    Molecular and Vascular Medicine Unit and Renal Division, Beth Israel Deaconess Medical Center E RW763, Boston, MA 02215, USA
    Am J Physiol Renal Physiol 292:F930-45. 2007
    ..7-PKD1(115-226) expression to accelerate decay of the flow-induced Ca(2+) signal in NK cells. These data provide evidence for ciliary dysfunction and polycystin mislocalization in human ADPKD cells with normal levels of PC1...
  32. doi request reprint Interactions of mouse glycophorin A with the dRTA-related mutant G719D of the mouse Cl-/HCO3- exchanger Ae1
    Andrew K Stewart
    Renal and Molecular Vascular Medicine Divisions, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Biochem Cell Biol 89:224-35. 2011
    ..However, the G719D mutation renders certain mAe1 mutant constructs GPA-unresponsive and highlights a role for erythroid-specific meAe1 amino acids 22-28 in GPA-responsiveness...
  33. ncbi request reprint Zebrafish slc4a2/ae2 anion exchanger: cDNA cloning, mapping, functional characterization, and localization
    Boris E Shmukler
    Molecular Medicine and Renal Units, Beth Israel Deaconess Med Ctr E RW763, 330 Brookline Ave, Boston, MA 02215, USA
    Am J Physiol Renal Physiol 289:F835-49. 2005
    ..These results will inform tests of the role of Ae2 in zebrafish kidney development and function...
  34. pmc Large conductance calcium-activated potassium channels contribute to the reduced myogenic tone of peripheral microvasculature after cardiopulmonary bypass
    Jun Feng
    Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA
    J Surg Res 157:123-8. 2009
    ..We investigated the role of calcium-activated potassium (K(Ca)) channel activation in myogenic tone in human peripheral microvasculature after heart surgery...
  35. pmc N-ethylmaleimide activates a Cl(-)-independent component of K(+) flux in mouse erythrocytes
    Boris E Shmukler
    Divisions of Nephrology and Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, USA
    Blood Cells Mol Dis 51:9-16. 2013
    ....
  36. pmc Strain-specific variations in cation content and transport in mouse erythrocytes
    Alicia Rivera
    Department of Laboratory Medicine, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Physiol Genomics 45:343-50. 2013
    ..These differences demonstrate the feasibility of identifying specific quantitative trait loci for erythroid ion transport and content in genetically standardized inbred mouse strains...
  37. pmc Cation-leak stomatocytosis in standard schnauzers does not cosegregate with coding mutations in the RhAG, SLC4A1, or GLUT1 genes associated with human disease
    Boris E Shmukler
    Dept of Medicine, Beth Israel Deaconess Med Ctr, Boston, MA 02215, United States
    Blood Cells Mol Dis 48:219-25. 2012
    ..The GLUT1 cDNA sequence was normal. We conclude that unlike human overhydrated cation-leak stomatocytosis, stomatocytosis in this cohort of Standard Schnauzers is not caused by mutations in the genes encoding RhAG, SLC4A1, or GLUT1...
  38. pmc Guanine nucleotides differentially modulate backbone dynamics of the STAS domain of the SulP/SLC26 transport protein Rv1739c of Mycobacterium tuberculosis
    Alok K Sharma
    Division of Molecular and Vascular Medicine, Renal Division, and Center for Vascular Biology Research, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA
    FEBS J 279:420-36. 2012
    ....
  39. pmc Increased sulfate uptake by E. coli overexpressing the SLC26-related SulP protein Rv1739c from Mycobacterium tuberculosis
    Alexander S Zolotarev
    Molecular and Vascular Medicine and Renal Divisions, Beth Israel Deaconess Medical Center, USA
    Comp Biochem Physiol A Mol Integr Physiol 149:255-66. 2008
    ..Our data show that facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, and suggest that Rv1739c may be a CysTWA-dependent sulfate transporter...
  40. pmc Hypoxia activates a Ca2+-permeable cation conductance sensitive to carbon monoxide and to GsMTx-4 in human and mouse sickle erythrocytes
    David H Vandorpe
    Molecular and Vascular Medicine Unit, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 5:e8732. 2010
    ..Deoxygenation-induced currents sharing some properties of Psickle have been recorded from sickle erythrocytes in whole cell configuration...
  41. pmc A conserved signal and GTPase complex are required for the ciliary transport of polycystin-1
    Heather H Ward
    Department of Pathology, Harvard Medical School, Boston, MA 02215, USA
    Mol Biol Cell 22:3289-305. 2011
    ..In this paper, we provide evidence for a conserved GTPase-dependent ciliary-trafficking mechanism that is shared between epithelia and neurons, and is essential in ciliary-trafficking and cell homeostasis...
  42. pmc Reduced DIDS-sensitive chloride conductance in Ae1-/- mouse erythrocytes
    Seth L Alper
    Molecular and Vascular Medicine Unit, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Blood Cells Mol Dis 41:22-34. 2008
    ..Thus, loss of one or more distinct, DIDS-sensitive anion channel polypeptide(s) from the Ae1(-/-) red cell membrane cannot be ruled out as an explanation for the reduced DIDS-sensitive anion conductance...
  43. pmc Loss-of-function and gain-of-function phenotypes of stomatocytosis mutant RhAG F65S
    Andrew K Stewart
    Beth Israel Deaconess Medical Center, 330 Brookline Ave, Boston, MA 02215, USA
    Am J Physiol Cell Physiol 301:C1325-43. 2011
    ..Thus, RhAG F65S is a loss-of-function mutation for amine transport. The altered oocyte intracellular pH, membrane potential, and currents associated with RhAG or RhAG F65S expression may reflect distinct transport mechanisms...
  44. ncbi request reprint Regulation of AE2 anion exchanger by intracellular pH: critical regions of the NH(2)-terminal cytoplasmic domain
    A K Stewart
    Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Boston 02215, USA
    Am J Physiol Cell Physiol 281:C1344-54. 2001
    ..Our data define structure-function relationships within the AE2 NH(2)-terminal cytoplasmic domain, which demonstrates distinct structural requirements for AE2 regulation by intracellular and extracellular protons...
  45. ncbi request reprint Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and functional regulation
    W Su
    Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Boston 02215, USA
    Am J Physiol 277:C899-912. 1999
    ..These reagents and findings will expedite studies of KCC1 structure-function relationships and of the pathobiology of KCC1-mediated K-Cl cotransport...
  46. pmc The SLC26 gene family of anion transporters and channels
    Seth L Alper
    Renal Division and Division of Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Mol Aspects Med 34:494-515. 2013
    ..The protein interactomes of SLC26 polypeptides are under active investigation...
  47. pmc Solution structure of the guanine nucleotide-binding STAS domain of SLC26-related SulP protein Rv1739c from Mycobacterium tuberculosis
    Alok K Sharma
    Divison of Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    J Biol Chem 286:8534-44. 2011
    ....
  48. pmc Molecular cloning and functional characterization of zebrafish Slc4a3/Ae3 anion exchanger
    Boris E Shmukler
    Renal Division and Molecular and Vascular Medicine Division, Beth Israel Deaconess Medical Center Department of Medicine, Harvard Medical School, 99 Brookline Ave RN 380 F, Boston, MA, 02215, USA
    Pflugers Arch 466:1605-18. 2014
    ..We conclude that multiple repeat elements have disrupted the 5'-end of the zebrafish ae3 gene, associated with N-terminal truncation of the protein and reduced anion transport activity...
  49. doi request reprint NMR assignment and secondary structure of the STAS domain of Rv1739c, a putative sulfate transporter of Mycobacterium tuberculosis
    Alok K Sharma
    Division of Molecular Vascular Medicine, Center for Vascular Biology Research, Beth Israel Deaconess Medical Center, RN 231 99 Brookline Ave, Boston, MA 02215, USA
    Biomol NMR Assign 3:99-102. 2009
    ..Rv1739c is a SulP anion permease, related in structure to the SLC26 gene family of metazoan anion exchangers and anion channels...
  50. ncbi request reprint Proteolytic cleavage sites of native AE2 anion exchanger in gastric mucosal membranes
    A S Zolotarev
    Molecular Medicine Unit, Beth Israel Hospital, Boston, Massachusetts 02215, USA
    Biochemistry 35:10367-76. 1996
    ..These results provide experimental confirmation for some aspects of AE2 topography previously predicted from primary structure alone...
  51. pmc Functional coupling of apical Cl-/HCO3- exchange with CFTR in stimulated HCO3- secretion by guinea pig interlobular pancreatic duct
    A K Stewart
    Renal Division, Molecular and Vascular Medicine Unit, Beth Israel Deaconess Med Ctr, 330 Brookline Ave, Boston, MA 02215, USA
    Am J Physiol Gastrointest Liver Physiol 296:G1307-17. 2009
    ..This study demonstrates functional coupling between Cftr and Slc26a6-like Cl(-)/HCO(3)(-) exchange activity in apical membrane of guinea pig pancreatic interlobular duct...
  52. pmc Acute regulation of mouse AE2 anion exchanger requires isoform-specific amino acid residues from most of the transmembrane domain
    A K Stewart
    Department of Medicine, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    J Physiol 584:59-73. 2007
    ....
  53. ncbi request reprint VHL tumor suppressor regulates Cl-/HCO3- exchange and Na+/H+ exchange activities in renal carcinoma cells
    S A Karumanchi
    Renal Division, Beth Israel Deaconess Medical Center, Departments of Medicine and Cell Biology, Harvard Medical School, Boston, Massachusetts 02215, USA
    Physiol Genomics 5:119-28. 2001
    ..AE2 and NHE3 are the first transcripts reported to be upregulated by pVHL. Elucidation of mechanisms responsible for downregulation of both ion exchange activities will require further investigation...
  54. pmc Cystic fibrosis transmembrane conductance regulator and caveolin-1 regulate epithelial cell internalization of Pseudomonas aeruginosa
    Milan Bajmoczi
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Am J Physiol Cell Physiol 297:C263-77. 2009
    ..Thus efficient initiation of innate immunity to P. aeruginosa requires formation of an epithelial "internalization platform" involving both caveolin-1 and functional, laterally mobile CFTR...
  55. ncbi request reprint Diarrhea-associated HIV-1 APIs potentiate muscarinic activation of Cl- secretion by T84 cells via prolongation of cytosolic Ca2+ signaling
    Paul A Rufo
    GI Cell Biology, Combined Program in Pediatric Gastroenterology and Nutrition, Children s Hospital, 300 Longwood Ave, Boston, MA 02115, USA
    Am J Physiol Cell Physiol 286:C998-C1008. 2004
    ..These data show that APIs modulate Ca(2+) signaling in secretory epithelial cells and identify a novel target for treatment of clinically important API side effects...
  56. ncbi request reprint Characterization of a highly polymorphic marker adjacent to the SLC4A1 gene and of kidney immunostaining in a family with distal renal tubular acidosis
    Chairat Shayakul
    Molecular Medicine Unit, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA
    Nephrol Dial Transplant 19:371-9. 2004
    ..Here, we characterized a polymorphic dinucleotide repeat close to the human AE1 gene and performed an immunocytochemical study of kidney tissue from a patient with inherited dRTA with a defined AE1 mutation...
  57. ncbi request reprint AE anion exchangers in atrial tumor cells
    P Papageorgiou
    Harvard-Thorndike Institute of Electrophysiology, Cardiovascular Division, Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Am J Physiol Heart Circ Physiol 280:H937-45. 2001
    ..Both bAE3 and cAE3 were present in intact AT-1 tumors. Cultured AT-1 cells provide a useful system for the study of mediators and regulators of Cl(-)/HCO(3)(-) exchange activity in an atrial cell type...
  58. ncbi request reprint Cation channel regulation by COOH-terminal cytoplasmic tail of polycystin-1: mutational and functional analysis
    David H Vandorpe
    Molecular Medicine, Beth Israel Deaconess Medical Center, Boston 02215, USA
    Physiol Genomics 8:87-98. 2002
    ..The divalent cation permeability and pharmacological profile of the current has been extended. Inducible expression of the PKD1 fusion in EcR-293 cells was also associated with activation of cation channels and increased Ca2+ entry...
  59. pmc SLC4A2-mediated Cl-/HCO3- exchange activity is essential for calpain-dependent regulation of the actin cytoskeleton in osteoclasts
    Fabienne Coury
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 110:2163-8. 2013
    ..These data suggest that maintenance of intracellular pH in osteoclasts through anion exchange regulates the actin superstructures required for bone resorption...
  60. ncbi request reprint Identification of a basolateral Cl-/HCO3- exchanger specific to gastric parietal cells
    Snezana Petrovic
    Department of Medicine, University of Cincinnati, Cincinnati 45267, USA
    Am J Physiol Gastrointest Liver Physiol 284:G1093-103. 2003
    ..On the basis of its unique expression pattern and function, we propose that SLC26A7 is a basolateral Cl(-)/HCO(3)(-) exchanger in gastric parietal cells and plays a major role in gastric acid secretion...
  61. pmc Molecular physiology and genetics of Na+-independent SLC4 anion exchangers
    Seth L Alper
    Renal Division and Molecular and Vascular Medicine Unit, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    J Exp Biol 212:1672-83. 2009
    ..A growing body of structure-function and interaction data, together with emerging information about physiological function and structure, is advancing our understanding of SLC4 anion exchangers...
  62. ncbi request reprint Familial renal tubular acidosis
    Seth L Alper
    Renal Division and Molecular and Vascular Medicine Division, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Nephrol 23:S57-76. 2010
    ..Additional RTA genes identified in knockout mice may lead to identification of additional human RTA genes...
  63. ncbi request reprint Endothelin-1 gene suppression by shear stress: pharmacological evaluation of the role of tyrosine kinase, intracellular calcium, cytoskeleton, and mechanosensitive channels
    A M Malek
    Department of Neurosurgery, Brigham and Women s Hospital, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    J Mol Cell Cardiol 31:387-99. 1999
    ....
  64. ncbi request reprint Transmembrane domain histidines contribute to regulation of AE2-mediated anion exchange by pH
    A K Stewart
    Beth Israel Deaconess Medical Center, 330 Brookline Ave, E RW763, Boston, MA 02215, USA
    Am J Physiol Cell Physiol 292:C909-18. 2007
    ..The results show that multiple TMD His residues contribute to basal AE2 activity and its sensitivity to pH(i) and pH(o)...
  65. ncbi request reprint Mannitol at clinical concentrations activates multiple signaling pathways and induces apoptosis in endothelial cells
    A M Malek
    Department of Neurosurgery, Brigham and Women s Hospital, Children s Hospital, and Harvard Medical School, Boston, Mass, USA
    Stroke 29:2631-40. 1998
    ..Though beneficial rheological effects commonly are attributed to mannitol, its direct effects on endothelial cells are poorly understood...
  66. ncbi request reprint Hemodynamic shear stress and its role in atherosclerosis
    A M Malek
    Neurosurgery, Brigham and Women s Hospital and Children s Hospital, Boston, Mass 02115, USA
    JAMA 282:2035-42. 1999
    ....
  67. ncbi request reprint cDNA cloning and functional characterization of the mouse Ca2+-gated K+ channel, mIK1. Roles in regulatory volume decrease and erythroid differentiation
    D H Vandorpe
    Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center Boston, Massachusetts 02215, USA
    J Biol Chem 273:21542-53. 1998
    ..Low nanomolar concentrations of CLT inhibited proliferation and erythroid differentiation of peripheral blood stem cells in liquid culture...
  68. ncbi request reprint Immunolocalization and tissue-specific splicing of AE2 anion exchanger in mouse kidney
    A K Stuart-Tilley
    Molecular Medicine Unit, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    J Am Soc Nephrol 9:946-59. 1998
    ..Unlike in rat, the mouse AE2c2 mRNA splice variant encoded a polypeptide with a novel predicted N-terminal amino acid sequence...
  69. ncbi request reprint Intracellular Ca(2+) signaling in endothelial cells by the angiogenesis inhibitors endostatin and angiostatin
    L Jiang
    Molecular Medicine and Renal Units and The Cancer Center, Beth Israel Deaconess Medical Center and Departments of Medicine and Cell Biology, Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Cell Physiol 280:C1140-50. 2001
    ..Intracellular Ca(2+) signaling may initiate or mediate some of the cellular actions of endostatin and angiostatin...
  70. ncbi request reprint Role of nonconserved charged residues of the AE2 transmembrane domain in regulation of anion exchange by pH
    A K Stewart
    Molecular and Vascular Medicine Unit and Renal Unit, Department of Medicine, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Pflugers Arch 454:373-84. 2007
    ....
  71. pmc The antifungal antibiotic, clotrimazole, inhibits chloride secretion by human intestinal T84 cells via blockade of distinct basolateral K+ conductances. Demonstration of efficacy in intact rabbit colon and in an in vivo mouse model of cholera
    P A Rufo
    Combined Program in Pediatric Gastroenterology and Nutrition, Children s Hospital, Boston, Massachusetts 02115, USA
    J Clin Invest 100:3111-20. 1997
    ..The results further support the suggestion that CLT and its metabolites may show clinical efficacy in the treatment of secretory diarrheas of diverse etiologies...
  72. pmc The antifungal antibiotic, clotrimazole, inhibits Cl- secretion by polarized monolayers of human colonic epithelial cells
    P A Rufo
    Combined Program in Pediatric Gastroenterology and Nutrition, Children s Hospital, Boston, Massachusetts 02115, USA
    J Clin Invest 98:2066-75. 1996
    ..CLT acts distal to the generation of cAMP and Ca2+ signals, and appears to inhibit basolateral K+ channels directly. CLT and related drugs may serve as novel antidiarrheal agents in humans and animals...
  73. pmc Therapy with oral clotrimazole induces inhibition of the Gardos channel and reduction of erythrocyte dehydration in patients with sickle cell disease
    C Brugnara
    Department of Laboratory Medicine, Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 97:1227-34. 1996
    ..This is the first in vivo evidence that the Gardos channel causes dehydration of sickle erythrocytes, and that its pharmacologic inhibition provides a realistic antisickling strategy...
  74. pmc Structural characterization of the C-terminal coiled-coil domains of wild-type and kidney disease-associated mutants of apolipoprotein L1
    Alok K Sharma
    Nephrology Division, Harvard Medical School, Boston, MA, USA
    FEBS J 283:1846-62. 2016
    ..These dynamic features of APOL1 CC variants likely reflect their intrinsic structural properties, and should help interpret future APOL1 structural studies and define the contribution of APOL1 risk variants to kidney disease. ..
  75. doi request reprint Molecular dynamics simulations of the STAS domains of rat prestin and human pendrin reveal conformational motions in conserved flexible regions
    Alok K Sharma
    Renal Division, Division of Molecular and Vascular Medicine, Center for Vascular Biology Research, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, MA, USA
    Cell Physiol Biochem 33:605-20. 2014
    ..We therefore performed a 100 ns 300K MD simulation study comparing the STAS domains of rat prestin and (modeled) human pendrin, to explore possible conformational flexibility in the region of the α1 and α2 helices...
  76. pmc STAS domain structure and function
    Alok K Sharma
    Molecular and Vascular Medicine Division, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Cell Physiol Biochem 28:407-22. 2011
    ..STAS domains are central to membrane targeting of many SulP/SLC26 anion transporters, and STAS domain mutations are associated with at least three human recessive diseases. This review summarizes STAS domain structure and function...
  77. ncbi request reprint AE2 anion exchanger polypeptide is a homooligomer in pig gastric membranes: a chemical cross-linking study
    A S Zolotarev
    Molecular Medicine Unit, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    Biochemistry 38:8521-31. 1999
    ..AE2 cross-linking could not be detected in membranes pretreated by hypotonic wash and freeze-thaw. The results are interpreted in light of the deduced amino acid sequence of the transmembrane domain of pig AE2...
  78. ncbi request reprint Molecular cloning, expression, and chromosomal localization of two isoforms of the AE3 anion exchanger from human heart
    D Yannoukakos
    Molecular Medicine Unit, Beth Israel Hospital, Boston, MA 02215
    Circ Res 75:603-14. 1994
    ..The human AE3 gene was localized to chromosome 2. AE3 may provide a new pharmacologic target for antiarrhythmic and cardioprotective drugs...
  79. pmc APOL1 kidney disease risk variants cause cytotoxicity by depleting cellular potassium and inducing stress-activated protein kinases
    Opeyemi A Olabisi
    Division of Nephrology, Department of Medicine, Massachusetts General Hospital, Boston, MA 02114 Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215 Harvard Medical School, Boston, MA 02215
    Proc Natl Acad Sci U S A 113:830-7. 2016
    ....
  80. pmc The Clinically Tested Gardos Channel Inhibitor Senicapoc Exhibits Antimalarial Activity
    Venée N Tubman
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA Division of Hematology Oncology, Boston Children s Hospital, Boston, Massachusetts, USA Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
    Antimicrob Agents Chemother 60:613-6. 2015
    ..Senicapoc treatment suppressed P. yoelii parasitemia in vivo in C57BL/6 mice. The reassuring safety and biochemical profile of senicapoc encourage its use in antimalarial development...
  81. pmc Transcriptional patterns in peritoneal tissue of encapsulating peritoneal sclerosis, a complication of chronic peritoneal dialysis
    Fabian R Reimold
    Renal Division, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 8:e56389. 2013
    ..The results are compatible with the hypothesis that encapsulating peritoneal sclerosis is a distinct pathological process from the simple peritoneal fibrosis that accompanies all PD treatment...
  82. ncbi request reprint Hypertonicity triggers RhoA-dependent assembly of myosin-containing striated polygonal actin networks in endothelial cells
    Adel M Malek
    Molecular and Vascular Medicine Unit, Beth Israel Deaconess Medical Center, 330 Brookline Ave, Boston, MA 02215, USA
    Am J Physiol Cell Physiol 292:C1645-59. 2007
    ....
  83. pmc K-Cl cotransporters, cell volume homeostasis, and neurological disease
    Kristopher T Kahle
    Department of Neurosurgery, Boston Children s Hospital and Harvard Medical School, Boston, MA 02114, USA Manton Center for Orphan Disease Research, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02114, USA Electronic address
    Trends Mol Med 21:513-23. 2015
    ..An improved understanding of WNK/SPAK-mediated KCC cell volume regulation in the nervous system might reveal novel avenues for the treatment of multiple neurological diseases. ..
  84. pmc Copy Number Variation at the APOL1 Locus
    Rupam Ruchi
    Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States of America Division of Nephrology, Department of Medicine, University of Florida, Gainesville, Florida, United States of America Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 10:e0125410. 2015
    ..Investigators and clinicians genotyping APOL1 should also consider whether the particular genotyping platform used is subject to technical errors when more than two copies of APOL1 are present. ..
  85. pmc Inhibition of WNK3 Kinase Signaling Reduces Brain Damage and Accelerates Neurological Recovery After Stroke
    Gulnaz Begum
    From the Department of Neurology, University of Pittsburgh, PA, G B, H Y, L L, S W, Y S, D S Department of Neurosurgery, Boston Children s Hospital K T K, Manton Center for Orphan Diseases K T K, and Department of Medicine B E S, S L A, Harvard Medical School, Boston, MA Renal Division and Vascular Biology Center, Beth Israel Deaconess Medical Center, Boston, MA B E S, S L A Division of Nephrology, Department of Medicine, Tri Service General Hospital, National Defense Medical Center, Taipei, Taiwan S S Y, S H L and Veterans Affairs Pittsburgh Health Care System, Geriatric Research, Educational and Clinical Center, PA D S
    Stroke 46:1956-65. 2015
    ..Here, we investigated the WNK3-SPAK/OSR1 pathway as a regulator of NKCC1 stimulation and their collective role in ischemic brain damage...
  86. pmc TNF-mediated damage to glomerular endothelium is an important determinant of acute kidney injury in sepsis
    Chang Xu
    Section of Nephrology, Department of Medicine, University of Chicago, Chicago, Illinois, USA
    Kidney Int 85:72-81. 2014
    ..Thus, our findings confirm the important role of glomerular endothelial injury, possibly by a decreased VEGF level, in the development and progression of AKI and albuminuria in the LPS model of sepsis in the mouse. ..
  87. pmc Calcium-activated potassium channels contribute to human skeletal muscle microvascular endothelial dysfunction related to cardiopulmonary bypass
    Yuhong Liu
    Division of Cardiothoracic Surgery, Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Surgery 144:239-44. 2008
    ..We investigated the role of calcium-activated potassium (K(Ca)) channel activity in human skeletal muscle microvascular function in the setting of cardiopulmonary bypass (CPB)...
  88. doi request reprint Let's look at cysts from both sides now
    Seth L Alper
    Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA
    Kidney Int 74:699-702. 2008
    ..1 K(+) channel, using a nontoxic small molecule with a close congener poised for rapid entry into the clinic. Cyst fluid accumulation can be blocked from both sides now...
  89. pmc Cellular actions and signaling by endostatin
    Ramani Ramchandran
    Division of Nephrology, Department of Medicine and Center for Study of the Tumor Microenvironment, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Crit Rev Eukaryot Gene Expr 12:175-91. 2002
    ..This review summarizes recent advances in endostatin biology and highlights new results on the cellular and subcellular mechanisms of endostatin action...
  90. ncbi request reprint Cell-specific mitotic defect and dyserythropoiesis associated with erythroid band 3 deficiency
    Barry H Paw
    Department of Medicine, Division of Hematology Oncology, Children s Hospital, Boston, Massachusetts, USA
    Nat Genet 34:59-64. 2003
    ..1R-binding domains. Our report establishes an evolutionarily conserved role for band 3 in erythroid-specific cell division and illustrates the concept of cell-specific adaptation for mitosis...
  91. ncbi request reprint The band 3-related anion exchanger (AE) gene family
    S L Alper
    Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts
    Annu Rev Physiol 53:549-64. 1991
  92. ncbi request reprint A dominant negative mutant of the KCC1 K-Cl cotransporter: both N- and C-terminal cytoplasmic domains are required for K-Cl cotransport activity
    S Casula
    Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    J Biol Chem 276:41870-8. 2001
    ..Delta(N)117 also exhibited dominant negative inhibition of human KCC1 and KCC3 and, with lower potency, mouse KCC4 and rat KCC2...
  93. ncbi request reprint The cytoplasmic C-terminal fragment of polycystin-1 regulates a Ca2+-permeable cation channel
    D H Vandorpe
    Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 276:4093-101. 2001
    ..Dysregulation of these or similar channels in autosomal dominant polycystic kidney disease may contribute to cyst formation or expansion...
  94. ncbi request reprint Structure and complex transcription pattern of the mouse SK1 K(Ca) channel gene, KCNN1
    B E Shmukler
    Molecular Medicine Unit, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA
    Biochim Biophys Acta 1518:36-46. 2001
    ..The evolutionary conservation of this complex transcription pattern suggests a possible role in the tuning of SK1 channel function...
  95. ncbi request reprint Structure and genetic polymorphism of the mouse KCC1 gene
    B E Shmukler
    Molecular Medicine Unit, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA
    Biochim Biophys Acta 1492:353-61. 2000
    ..These and additional described strain-specific polymorphisms will be useful mapping and genetic tools in the study of mouse models of sickle cell disease...
  96. ncbi request reprint Mice with a targeted disruption of the AE2 Cl-/HCO3- exchanger are achlorhydric
    Lara R Gawenis
    Department of Molecular Genetics, Biochemistry and Microbiology, Unniversity of Cincinnati College of Medicine, Ohio 45267, USA
    J Biol Chem 279:30531-9. 2004
    ..These results demonstrate that AE2 is essential for gastric acid secretion and for normal development of secretory canalicular and tubulovesicular membranes in mouse parietal cells...
  97. ncbi request reprint Cl(-)/HCO(3)(-) exchange is acetazolamide sensitive and activated by a muscarinic receptor-induced [Ca(2+)](i) increase in salivary acinar cells
    Ha Van Nguyen
    Center for Oral Biology, University of Rochester Medical Center, Rochester, New York 14642, USA
    Am J Physiol Gastrointest Liver Physiol 286:G312-20. 2004
    ..Moreover, muscarinic receptor stimulation enhanced AE activity through a Ca(2+)-dependent mechanism. Such forms of regulation may play important roles in modulating fluid and electrolyte secretion by salivary gland acinar cells...
  98. ncbi request reprint Acute pH-dependent regulation of AE2-mediated anion exchange involves discrete local surfaces of the NH2-terminal cytoplasmic domain
    Andrew K Stewart
    Burdon Sanderson Cardiac Science Centre, University Laboratory of Physiology, University of Oxford, Parks Road, Oxford OX1 3PT, United Kingdom
    J Biol Chem 279:52664-76. 2004
    ....
  99. ncbi request reprint Enhanced formation of a HCO3- transport metabolon in exocrine cells of Nhe1-/- mice
    Mireya Gonzalez-Begne
    Center for Oral Biology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 282:35125-32. 2007
    ....
  100. ncbi request reprint Interactions of transmembrane carbonic anhydrase, CAIX, with bicarbonate transporters
    Patricio E Morgan
    Membrane Protein Research Group, Dept of Physiology, University of Alberta, Edmonton, Alberta, Canada
    Am J Physiol Cell Physiol 293:C738-48. 2007
    ..We conclude that CAIX can bind to some Cl(-)/HCO(3)(-) exchangers to form a bicarbonate transport metabolon...
  101. ncbi request reprint Effect of chronic elevated carbon dioxide on the expression of acid-base transporters in the neonatal and adult mouse
    Amjad Kanaan
    Department of Pediatrics, Section of Respiratory Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Am J Physiol Regul Integr Comp Physiol 293:R1294-302. 2007
    ....