Kenji Fukasawa

Summary

Affiliation: H. Lee Moffitt Cancer Center and Research Institute
Country: USA

Publications

  1. ncbi request reprint Oncogenes and tumour suppressors take on centrosomes
    Kenji Fukasawa
    Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, Florida 33612, USA
    Nat Rev Cancer 7:911-24. 2007
  2. pmc RhoA and RhoC are both required for the ROCK II-dependent promotion of centrosome duplication
    M Kanai
    Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA
    Oncogene 29:6040-50. 2010
  3. doi request reprint Aberrant activation of cell cycle regulators, centrosome amplification, and mitotic defects
    Kenji Fukasawa
    Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA
    Horm Cancer 2:104-12. 2011
  4. pmc P53, cyclin-dependent kinase and abnormal amplification of centrosomes
    Kenji Fukasawa
    Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA
    Biochim Biophys Acta 1786:15-23. 2008
  5. ncbi request reprint Inhibition of Crm1-p53 interaction and nuclear export of p53 by poly(ADP-ribosyl)ation
    Masayuki Kanai
    H Lee Moffitt Cancer Center and Research Institute, Tampa, Florida 33612, USA
    Nat Cell Biol 9:1175-83. 2007
  6. ncbi request reprint Suppression of centrosome amplification after DNA damage depends on p27 accumulation
    Eiji Sugihara
    Nagahama Institute of Bio Science and Technology, Shiga, Japan
    Cancer Res 66:4020-9. 2006
  7. ncbi request reprint Centrosome amplification, chromosome instability and cancer development
    Kenji Fukasawa
    Department of Cell Biology, University of Cincinnati College of Medicine, P O Box 670521 3125 Eden Ave, Cincinnati, OH 45267 0521, USA
    Cancer Lett 230:6-19. 2005
  8. pmc Involvement of Crm1 in hepatitis B virus X protein-induced aberrant centriole replication and abnormal mitotic spindles
    Marshonna Forgues
    Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute NIH, 37 Convent Drive, Bethesda, MD 20892, USA
    Mol Cell Biol 23:5282-92. 2003
  9. ncbi request reprint Induction of centrosome amplification and chromosome instability in human bladder cancer cells by p53 mutation and cyclin E overexpression
    Kenji Kawamura
    Department of Cell Biology, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH 45267, USA
    Cancer Res 64:4800-9. 2004
  10. ncbi request reprint Introduction. Centrosome
    Kenji Fukasawa
    Department of Cell Biology, University of Cincinnati College of Medicine, PO Box 670521, Cincinnati, Ohio, OH 45267 0521, USA
    Oncogene 21:6140-5. 2002

Collaborators

  • Steven P Angus
  • Michael J Difilippantonio
  • K Kawamura
  • Masaru Okuda
  • James A Fagin
  • Erik S Knudsen
  • James V DeGregori
  • Masayuki Kanai
  • Zhiyong Ma
  • Pheruza Tarapore
  • Masanao Miwa
  • Eiji Sugihara
  • M Kanai
  • Song Hee Kim
  • Jeffrey A Knauf
  • Hideki Izumi
  • Kazuya Shinmura
  • Tatsuo Oikawa
  • Richard A Bennett
  • Yukari Tokuyama
  • Harold I Saavedra
  • Marshonna Forgues
  • M S Crowe
  • G F Vande Woude
  • Y Zheng
  • Shuji Hanai
  • A Hamid Boulares
  • Mark Winey
  • Christopher P Mattison
  • Kazuhiko Hanashiro
  • Manfred Schwab
  • Keiko Nakayama
  • Hideyuki Saya
  • Keiichi I Nakayama
  • George Babcock
  • Kozo Kaibuchi
  • Keqiang Ye
  • Soichiro Saito
  • Hideo Toyoshima
  • Takayuki Nitta
  • Bin Ouyang
  • Hajime Tsujimoto
  • Hisashi Inokuma
  • Rakesh Goorha
  • Kyle R George
  • Xin W Wang
  • Baidehi Maiti
  • Rachel Altura
  • Wei Min Tong
  • Kunio Nagashima
  • Jeffrey Feden
  • Thomas Ried
  • Cynthia Timmers
  • Kristoffer Valerie
  • Zhao Qi Wang
  • Gustavo Leone
  • Steven P Linke

Detail Information

Publications21

  1. ncbi request reprint Oncogenes and tumour suppressors take on centrosomes
    Kenji Fukasawa
    Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, Florida 33612, USA
    Nat Rev Cancer 7:911-24. 2007
    ..How these proteins control centrosome duplication and function, and how their mutational activation and/or inactivation results in numeral and functional centrosome abnormalities, is discussed in this Review...
  2. pmc RhoA and RhoC are both required for the ROCK II-dependent promotion of centrosome duplication
    M Kanai
    Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA
    Oncogene 29:6040-50. 2010
    ..We found that both RhoA and RhoC, but not RhoB, were required for initiation of centrosome duplication, and overactivation of RhoA, as well as RhoC, but not RhoB, promoted centrosome duplication and centrosome amplification...
  3. doi request reprint Aberrant activation of cell cycle regulators, centrosome amplification, and mitotic defects
    Kenji Fukasawa
    Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA
    Horm Cancer 2:104-12. 2011
    ..These observations reveal an unexplored, yet important, oncogenic activities of those receptors in carcinogenesis; destabilizing chromosomes through promotion of centrosome amplification via continual activation of the Rho-ROCK2 pathway...
  4. pmc P53, cyclin-dependent kinase and abnormal amplification of centrosomes
    Kenji Fukasawa
    Molecular Oncology Program, H Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA
    Biochim Biophys Acta 1786:15-23. 2008
    ..In this review, the role of centrosome amplification in tumor progression, and mechanistic view of how centrosomes are amplified in cells through focusing on loss of p53 and aberrant activities of CDK2-cyclins will be discussed...
  5. ncbi request reprint Inhibition of Crm1-p53 interaction and nuclear export of p53 by poly(ADP-ribosyl)ation
    Masayuki Kanai
    H Lee Moffitt Cancer Center and Research Institute, Tampa, Florida 33612, USA
    Nat Cell Biol 9:1175-83. 2007
    ..The nuclear export machinery is unable to target poly(ADP-ribosyl)ated p53, promoting accumulation of p53 in the nucleus where p53 exerts its transactivational function...
  6. ncbi request reprint Suppression of centrosome amplification after DNA damage depends on p27 accumulation
    Eiji Sugihara
    Nagahama Institute of Bio Science and Technology, Shiga, Japan
    Cancer Res 66:4020-9. 2006
    ..These results suggest that the accumulation of p27 after DNA damage is required for suppression of centrosome amplification, thereby preventing chromosomal instability...
  7. ncbi request reprint Centrosome amplification, chromosome instability and cancer development
    Kenji Fukasawa
    Department of Cell Biology, University of Cincinnati College of Medicine, P O Box 670521 3125 Eden Ave, Cincinnati, OH 45267 0521, USA
    Cancer Lett 230:6-19. 2005
    ..In this review, how centrosome amplification destabilizes chromosomes, how loss of certain tumor suppressor proteins leads to centrosome amplification, and the role of centrosome amplification in cancer development will be discussed...
  8. pmc Involvement of Crm1 in hepatitis B virus X protein-induced aberrant centriole replication and abnormal mitotic spindles
    Marshonna Forgues
    Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute NIH, 37 Convent Drive, Bethesda, MD 20892, USA
    Mol Cell Biol 23:5282-92. 2003
    ..Based on this evidence, we suggest that Crm1 is actively involved in maintaining centrosome integrity and that HBx disrupts this process by inactivating Crm1 and thus contributes to HBV-mediated carcinogenesis...
  9. ncbi request reprint Induction of centrosome amplification and chromosome instability in human bladder cancer cells by p53 mutation and cyclin E overexpression
    Kenji Kawamura
    Department of Cell Biology, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH 45267, USA
    Cancer Res 64:4800-9. 2004
    ....
  10. ncbi request reprint Introduction. Centrosome
    Kenji Fukasawa
    Department of Cell Biology, University of Cincinnati College of Medicine, PO Box 670521, Cincinnati, Ohio, OH 45267 0521, USA
    Oncogene 21:6140-5. 2002
  11. ncbi request reprint Loss of p53 and centrosome hyperamplification
    Pheruza Tarapore
    Department of Cell Biology, University of Cincinnati College of Medicine PO Box 670521, Cincinnati, Ohio, OH 45267 0521, USA
    Oncogene 21:6234-40. 2002
    ..p53 appears to exert its transactivation-independent control through direct physical binding to the centrosomes...
  12. ncbi request reprint A mammalian in vitro centriole duplication system: evidence for involvement of CDK2/cyclin E and nucleophosmin/B23 in centrosome duplication
    Pheruza Tarapore
    Department of Cell Biology, University of Cincinnati College of Medicine, P O Box 670521, Ohio 45267 0521, USA
    Cell Cycle 1:75-81. 2002
    ....
  13. pmc Involvement of poly(ADP-Ribose) polymerase 1 and poly(ADP-Ribosyl)ation in regulation of centrosome function
    Masayuki Kanai
    Department of Biochemistry and Molecular Oncology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305 8575, Japan
    Mol Cell Biol 23:2451-62. 2003
    ..These results indicate that PARP-1 and PARP-1-mediated poly(ADP-ribosyl)ation of centrosomal proteins are involved in the regulation of centrosome function...
  14. ncbi request reprint Physical and functional interaction between mortalin and Mps1 kinase
    Masayuki Kanai
    Department of Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA
    Genes Cells 12:797-810. 2007
    ..Moreover, Mps1-associated acceleration of centrosome duplication depends on the presence of mortalin and super-activation by the Thr62/Ser65 phosphorylated mortalin...
  15. pmc Interaction between ROCK II and nucleophosmin/B23 in the regulation of centrosome duplication
    Zhiyong Ma
    Department of Cell Biology, University of Cincinnati College of Medicine, 3125 Eden Avenue, Cincinnati, OH 45267 0521, USA
    Mol Cell Biol 26:9016-34. 2006
    ..All these findings point to ROCK II as the effector of the CDK2/cyclin E-NPM/B23 pathway in the regulation of centrosome duplication...
  16. pmc Inactivation of E2F3 results in centrosome amplification
    Harold I Saavedra
    Human Cancer Genetics Program, Department of Molecular Virology, Immunology and Medical Genetics, Children s Research Institute, The Ohio State University, Columbus, OH 43210, USA
    Cancer Cell 3:333-46. 2003
    ..Our findings implicate the E2F3 transcription factor as an important link that orchestrates DNA and centrosome duplication cycles, ensuring the faithful transmission of genetic material to daughter cells...
  17. ncbi request reprint Oncogenic RAS induces accelerated transition through G2/M and promotes defects in the G2 DNA damage and mitotic spindle checkpoints
    Jeffrey A Knauf
    Division of Endocrinology and Metabolism, University of Cincinnati College of Medicine, OH 45267, USA
    J Biol Chem 281:3800-9. 2006
    ..We propose that oncogenic RAS activation may predispose cells to genomic instability through both MAPK-dependent and independent pathways that affect critical checkpoints in G(2)/M...
  18. ncbi request reprint Characterization of centrosomal association of nucleophosmin/B23 linked to Crm1 activity
    Kazuya Shinmura
    Department of Cell Biology, University of Cincinnati College of Medicine, P O Box 670521 3125 Eden Avenue, Cincinnati, OH 45267 0521, United States
    FEBS Lett 579:6621-34. 2005
    ..We further found that inhibition of Crm1 nuclear export receptor results in both accumulation of cyclin E at centrosomes and efficient dissociation of NPM/B23 from centrosomes...
  19. pmc Transcriptional control of BubR1 by p53 and suppression of centrosome amplification by BubR1
    Tatsuo Oikawa
    Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, 1677 1 Yoshida, Yamaguchi 753 8515, Japan
    Mol Cell Biol 25:4046-61. 2005
    ..Our studies demonstrate the molecular aspect of genomic convergence in cultured cells, providing critical information for understanding the stepwise progression of tumors...
  20. ncbi request reprint Induction of centrosome amplification and chromosome instability in p53-null cells by transient exposure to subtoxic levels of S-phase-targeting anticancer drugs
    Richard A Bennett
    Department of Cell Biology, University of Cincinnati College of Medicine, PO Box 670521, Cincinnati, OH 45267 0521, USA
    Oncogene 23:6823-9. 2004
    ..This in turn promotes generation of tumor cells equipped with further malignant characteristics...
  21. ncbi request reprint Active RB elicits late G1/S inhibition
    Steven P Angus
    Department of Cell Biology, University of Cincinnati College of Medicine, Vontz Center for Molecular Studies, Cincinnati, Ohio 45267 0521, USA
    Exp Cell Res 276:201-13. 2002
    ..These studies indicate that RB inhibits cell cycle progression by targeting CDK2/cyclin A-dependent events at the G1/S transition to inhibit cell cycle progression...

Research Grants16

  1. Function of Nucleophosmin/B23 in Centrosome Duplication
    Kenji Fukasawa; Fiscal Year: 2006
    ..Moreover, blocking the centrosome duplication process results in suppression of chromosome instability as well as cell division. ..
  2. P53 Mediated Regulation of Centrosome Duplication
    Kenji Fukasawa; Fiscal Year: 2001
    ..Moreover, blocking the centrosome duplication process results in suppression of chromosome instability as well as cell division. ..
  3. p53-mediated control of numeral integrity of centrosomes
    Kenji Fukasawa; Fiscal Year: 2010
    ..Moreover, targeted inhibition of centrosome duplication will not only block cell division, but also suppress chromosome instability. ..
  4. p53-mediated control of numeral integrity of centrosomes
    Kenji Fukasawa; Fiscal Year: 2009
    ..Moreover, targeted inhibition of centrosome duplication will not only block cell division, but also suppress chromosome instability. ..
  5. p53-mediated control of numeral integrity of centrosomes
    Kenji Fukasawa; Fiscal Year: 2007
    ..Moreover, targeted inhibition of centrosome duplication will not only block cell division, but also suppress chromosome instability. ..
  6. p53-mediated control of numeral integrity of centrosomes
    Kenji Fukasawa; Fiscal Year: 2006
    ..Moreover, targeted inhibition of centrosome duplication will not only block cell division, but also suppress chromosome instability. ..
  7. P53 Mediated Regulation of Centrosome Duplication
    Kenji Fukasawa; Fiscal Year: 2005
    ..Moreover, blocking the centrosome duplication process results in suppression of chromosome instability as well as cell division. ..
  8. Function of Nucleophosmin/B23 in Centrosome Duplication
    Kenji Fukasawa; Fiscal Year: 2005
    ..Moreover, blocking the centrosome duplication process results in suppression of chromosome instability as well as cell division. ..
  9. P53 Mediated Regulation of Centrosome Duplication
    Kenji Fukasawa; Fiscal Year: 2004
    ..Moreover, blocking the centrosome duplication process results in suppression of chromosome instability as well as cell division. ..
  10. Function of Nucleophosmin/B23 in Centrosome Duplication
    Kenji Fukasawa; Fiscal Year: 2004
    ..Moreover, blocking the centrosome duplication process results in suppression of chromosome instability as well as cell division. ..
  11. P53 Mediated Regulation of Centrosome Duplication
    Kenji Fukasawa; Fiscal Year: 2003
    ..Moreover, blocking the centrosome duplication process results in suppression of chromosome instability as well as cell division. ..
  12. Function of Nucleophosmin/B23 in Centrosome Duplication
    Kenji Fukasawa; Fiscal Year: 2003
    ..Moreover, blocking the centrosome duplication process results in suppression of chromosome instability as well as cell division. ..
  13. Function of Nucleophosmin/B23 in Centrosome Duplication
    Kenji Fukasawa; Fiscal Year: 2002
    ..Moreover, blocking the centrosome duplication process results in suppression of chromosome instability as well as cell division. ..
  14. ROCK II-mediated regulation of centrosome duplication / centrosome amplification
    Kenji Fukasawa; Fiscal Year: 2010
    ....
  15. P53 Mediated Regulation of Centrosome Duplication
    Kenji Fukasawa; Fiscal Year: 2002
    ..Moreover, blocking the centrosome duplication process results in suppression of chromosome instability as well as cell division. ..