Manu O Platt

Summary

Affiliation: Georgia Institute of Technology
Country: USA

Publications

  1. pmc Endothelial cells and cathepsins: Biochemical and biomechanical regulation
    Manu O Platt
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 950 Atlantic Drive, Suite 3015, Atlanta, GA 30332, USA Electronic address
    Biochimie 122:314-23. 2016
  2. pmc Low-Cost Method to Monitor Patient Adherence to HIV Antiretroviral Therapy Using Multiplex Cathepsin Zymography
    Manu O Platt
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 315 Ferst Drive, IBB 1308, Atlanta, GA, 30332, USA
    Mol Biotechnol 58:56-64. 2016
  3. pmc Sickle cell disease activates peripheral blood mononuclear cells to induce cathepsins k and v activity in endothelial cells
    Philip M Keegan
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Anemia 2012:201781. 2012
  4. ncbi request reprint T-cell phosphokinome as a fingerprint of effective graft versus leukemia
    Manu O Platt
    Wallace H Coulter Department of Biomedical Engineering and Institute for Bioengineering and Biosciences, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Front Biosci (Elite Ed) 4:721-33. 2012
  5. pmc Tumor necrosis factor alpha stimulates cathepsin K and V activity via juxtacrine monocyte-endothelial cell signaling and JNK activation
    Philip M Keegan
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Mol Cell Biochem 367:65-72. 2012
  6. pmc Patient specific proteolytic activity of monocyte-derived macrophages and osteoclasts predicted with temporal kinase activation states during differentiation
    Keon Young Park
    Department of Biomedical Engineering and Parker H Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology and Emory University, 315 Ferst Drive, Atlanta, GA 30332, USA
    Integr Biol (Camb) 4:1459-69. 2012
  7. pmc Pro-atherogenic shear stress and HIV proteins synergistically upregulate cathepsin K in endothelial cells
    Ivana Kennedy Parker
    George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA
    Ann Biomed Eng 42:1185-94. 2014
  8. ncbi request reprint Expression of cathepsin K is regulated by shear stress in cultured endothelial cells and is increased in endothelium in human atherosclerosis
    Manu O Platt
    Wallace H Coulter Dept of Biomedical Engineering at Georgia Tech and Emory Univ, 308D WMB, Atlanta, GA 30322, USA
    Am J Physiol Heart Circ Physiol 292:H1479-86. 2007
  9. pmc Manipulating substrate and pH in zymography protocols selectively distinguishes cathepsins K, L, S, and V activity in cells and tissues
    Catera L Wilder
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, 30332, USA
    Arch Biochem Biophys 516:52-7. 2011
  10. pmc Azidothymidine (AZT) leads to arterial stiffening and intima-media thickening in mice
    Laura Hansen
    The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA
    J Biomech 46:1540-7. 2013

Collaborators

Detail Information

Publications29

  1. pmc Endothelial cells and cathepsins: Biochemical and biomechanical regulation
    Manu O Platt
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 950 Atlantic Drive, Suite 3015, Atlanta, GA 30332, USA Electronic address
    Biochimie 122:314-23. 2016
    ....
  2. pmc Low-Cost Method to Monitor Patient Adherence to HIV Antiretroviral Therapy Using Multiplex Cathepsin Zymography
    Manu O Platt
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 315 Ferst Drive, IBB 1308, Atlanta, GA, 30332, USA
    Mol Biotechnol 58:56-64. 2016
    ..Low cost of this electrophoresis-based assay makes it a prime candidate for implementation in resource-limited settings. ..
  3. pmc Sickle cell disease activates peripheral blood mononuclear cells to induce cathepsins k and v activity in endothelial cells
    Philip M Keegan
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Anemia 2012:201781. 2012
    ....
  4. ncbi request reprint T-cell phosphokinome as a fingerprint of effective graft versus leukemia
    Manu O Platt
    Wallace H Coulter Department of Biomedical Engineering and Institute for Bioengineering and Biosciences, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Front Biosci (Elite Ed) 4:721-33. 2012
    ..Higher throughput methods to characterize these signals and different model systems will be discussed, along with benefits and challenges of using the T cell phosphokinome as a fingerprint to predict GvHD and GvL...
  5. pmc Tumor necrosis factor alpha stimulates cathepsin K and V activity via juxtacrine monocyte-endothelial cell signaling and JNK activation
    Philip M Keegan
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Mol Cell Biochem 367:65-72. 2012
    ....
  6. pmc Patient specific proteolytic activity of monocyte-derived macrophages and osteoclasts predicted with temporal kinase activation states during differentiation
    Keon Young Park
    Department of Biomedical Engineering and Parker H Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology and Emory University, 315 Ferst Drive, Atlanta, GA 30332, USA
    Integr Biol (Camb) 4:1459-69. 2012
    ..Such analyses may provide beneficial tools for personalized kinase and protease inhibitor therapies for tissue destructive diseases...
  7. pmc Pro-atherogenic shear stress and HIV proteins synergistically upregulate cathepsin K in endothelial cells
    Ivana Kennedy Parker
    George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA
    Ann Biomed Eng 42:1185-94. 2014
    ....
  8. ncbi request reprint Expression of cathepsin K is regulated by shear stress in cultured endothelial cells and is increased in endothelium in human atherosclerosis
    Manu O Platt
    Wallace H Coulter Dept of Biomedical Engineering at Georgia Tech and Emory Univ, 308D WMB, Atlanta, GA 30322, USA
    Am J Physiol Heart Circ Physiol 292:H1479-86. 2007
    ..These findings suggest that cathepsin K is a mechanosensitive, extracellular matrix protease that, in turn, may be involved in arterial wall remodeling and atherosclerosis...
  9. pmc Manipulating substrate and pH in zymography protocols selectively distinguishes cathepsins K, L, S, and V activity in cells and tissues
    Catera L Wilder
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, 30332, USA
    Arch Biochem Biophys 516:52-7. 2011
    ....
  10. pmc Azidothymidine (AZT) leads to arterial stiffening and intima-media thickening in mice
    Laura Hansen
    The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA
    J Biomech 46:1540-7. 2013
    ..Thus, this study suggests the drug AZT has significant effects on the development of subclinical markers of atherosclerosis...
  11. ncbi request reprint Cyclic pressure and shear stress regulate matrix metalloproteinases and cathepsin activity in porcine aortic valves
    Manu O Platt
    The Wallace H Coulter School of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA
    J Heart Valve Dis 15:622-9. 2006
    ..It was hypothesized that exposure of the aortic valves to pressure or shear stress would regulate valvular expression of proteases such as matrix metalloproteinases (MMPs) and cathepsins...
  12. ncbi request reprint Laminar shear stress inhibits cathepsin L activity in endothelial cells
    Manu O Platt
    Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Emory University, 308D WMB, Atlanta, GA 30322, USA
    Arterioscler Thromb Vasc Biol 26:1784-90. 2006
    ..It is not known, however, whether shear regulates cathepsins. We examined the hypothesis that shear stress regulates cathepsin activity in endothelial cells...
  13. pmc Cathepsin S cannibalism of cathepsin K as a mechanism to reduce type I collagen degradation
    Zachary T Barry
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA
    J Biol Chem 287:27723-30. 2012
    ..Together, these results provide new insight into the combined proteolytic activities of cathepsins toward substrates and each other and present kinetic models to consider for more accurate predictions and descriptions of these systems...
  14. pmc MMP-mediated mesenchymal morphogenesis of pluripotent stem cell aggregates stimulated by gelatin methacrylate microparticle incorporation
    Anh H Nguyen
    The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA
    Biomaterials 76:66-75. 2016
    ..We speculate that controlling protease activity via the introduction of ECM-based materials may offer a novel route to engineer the ECM microenvironment to modulate stem cell differentiation. ..
  15. pmc Endothelial dysfunction, arterial stiffening, and intima-media thickening in large arteries from HIV-1 transgenic mice
    Laura Hansen
    The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA
    Ann Biomed Eng 41:682-93. 2013
    ..These findings are consistent with the hypothesis that HIV-proteins, independent of HAART treatment or HIV infection, could play a role in of the development of CVD...
  16. doi request reprint Cathepsin Protease Inhibition Reduces Endometriosis Lesion Establishment
    Kristi M Porter
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia
    Reprod Sci 23:623-9. 2016
    ....
  17. ncbi request reprint Simulated microgravity using the Random Positioning Machine inhibits differentiation and alters gene expression profiles of 2T3 preosteoblasts
    Steven J Pardo
    Wallace H Coulter Dept of Biomedical Engineering, Georgia Institute of Technology and Emory University, 308D WMB, Atlanta, GA 30322, USA
    Am J Physiol Cell Physiol 288:C1211-21. 2005
    ....
  18. doi request reprint Efavirenz treatment causes arterial stiffening in apolipoprotein E-null mice
    Alexander W Caulk
    The George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 315 Ferst Drive, IBB 2305, Atlanta, GA 30332, USA
    J Biomech 48:2176-80. 2015
    ..Taken together, these data suggest that EFV leads to arterial stiffening but, for the dose and duration tested, did not lead to elevated plaque progression in ApoE(-/-) mice...
  19. pmc Cathepsins in Rotator Cuff Tendinopathy: Identification in Human Chronic Tears and Temporal Induction in a Rat Model
    Song P Seto
    W H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, Atlanta, GA, 30332, USA
    Ann Biomed Eng 43:2036-46. 2015
    ..These results provide important information on a yet unexplored mechanism for tendon degeneration that may operate alone or in conjunction with other proteases to contribute to chronic tendinopathy. ..
  20. pmc Systematic optimization of multiplex zymography protocol to detect active cathepsins K, L, S, and V in healthy and diseased tissue: compromise among limits of detection, reduced time, and resources
    Jerald E Dumas
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 315 Ferst Dr Suite 1308, Atlanta, GA 30332, USA
    Mol Biotechnol 54:1038-47. 2013
    ....
  21. doi request reprint Detection of femtomole quantities of mature cathepsin K with zymography
    Weiwei A Li
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Anal Biochem 401:91-8. 2010
    ..In summary, this inexpensive, species-independent, antibody-free protocol describes a sensitive method with broad potential to elucidate previously undetectable cathepsin K activity...
  22. doi request reprint Supraspinatus tendon overuse results in degenerative changes to tendon insertion region and adjacent humeral cartilage in a rat model
    Akia N Parks
    W H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, Atlanta, Georgia 30332
    J Orthop Res . 2016
    ..2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res...
  23. pmc Muscadine grape skin extract can antagonize Snail-cathepsin L-mediated invasion, migration and osteoclastogenesis in prostate and breast cancer cells
    Liza J Burton
    Department of Biological Sciences, Center for Cancer Research and Therapeutic Development, Clark Atlanta University, Atlanta, GA 30314, USA Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30322, USA and Department of Medicine, Howard University, Washington, DC 20060, USA
    Carcinogenesis 36:1019-27. 2015
    ..Therefore, inhibition using a natural product such as MSKE could potentially be a promising bioactive compound for bone metastatic cancer. ..
  24. pmc Monocyte-derived macrophage assisted breast cancer cell invasion as a personalized, predictive metric to score metastatic risk
    Keon Young Park
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332
    Sci Rep 5:13855. 2015
    ....
  25. pmc Multiplex zymography captures stage-specific activity profiles of cathepsins K, L, and S in human breast, lung, and cervical cancer
    Binbin Chen
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, GA 30332, Atlanta, USA
    J Transl Med 9:109. 2011
    ..Here, we have developed multiplex cathepsin zymography to profile cathepsins K, L, and S activity in 10 μg human breast, lung, and cervical tumors by exploiting unique electrophoretic mobility and renaturation properties...
  26. doi request reprint Sickle cell biomechanics
    Gilda A Barabino
    The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA
    Annu Rev Biomed Eng 12:345-67. 2010
    ..This review discusses the characterization of the biomechanical properties of sickle RBCs and sickle blood as well as their implications toward a better understanding of the pathophysiology of the disease...
  27. ncbi request reprint Bone morphogenic protein 4 produced in endothelial cells by oscillatory shear stress induces monocyte adhesion by stimulating reactive oxygen species production from a nox1-based NADPH oxidase
    George P Sorescu
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30322, USA
    Circ Res 95:773-9. 2004
    ..Together, these results suggest that BMP4 produced in ECs by OS stimulates ROS release from the nox1-dependent NADPH oxidase leading to inflammation, a critical early atherogenic step...
  28. pmc Differential cathepsin responses to inhibitor-induced feedback: E-64 and cystatin C elevate active cathepsin S and suppress active cathepsin L in breast cancer cells
    Catera L Wilder
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Int J Biochem Cell Biol 79:199-208. 2016
    ..This can confound predictions based solely on inhibitor kinetics, and must be better understood to effectively deploy therapies and dosing strategies that target cathepsins to prevent cancer progression...
  29. pmc PACMANS: A Bioinformatically Informed Algorithm to Predict, Design, and Disrupt Protease-on-Protease Hydrolysis
    Meghan C Ferrall-Fairbanks
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA
    Protein Sci . 2017
    ..This article is protected by copyright. All rights reserved...