Sanjay Adhikari

Summary

Affiliation: Georgetown University
Country: USA

Publications

  1. ncbi request reprint Dipole-dipole interaction stabilizes the transition state of apurinic/apyrimidinic endonuclease--abasic site interaction
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, 3800 Reservoir Road NW, Washington, D C 20057, USA
    J Biol Chem 283:1334-9. 2008
  2. pmc Evidence of complete cellular repair of 1,N6-ethenoadenine, a mutagenic and potential damage for human cancer, revealed by a novel method
    Sujata Choudhury
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    Mol Cell Biochem 313:19-28. 2008
  3. pmc Characterization of magnesium requirement of human 5'-tyrosyl DNA phosphodiesterase mediated reaction
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    BMC Res Notes 5:134. 2012
  4. pmc A unified method for purification of basic proteins
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    Anal Biochem 400:203-6. 2010
  5. pmc Development of a novel assay for human tyrosyl DNA phosphodiesterase 2
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    Anal Biochem 416:112-6. 2011
  6. pmc Excised damaged base determines the turnover of human N-methylpurine-DNA glycosylase
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, United States
    DNA Repair (Amst) 8:1201-6. 2009
  7. ncbi request reprint N-terminal extension of N-methylpurine DNA glycosylase is required for turnover in hypoxanthine excision reaction
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, D C 20057, USA
    J Biol Chem 282:30078-84. 2007
  8. pmc Germ line variants of human N-methylpurine DNA glycosylase show impaired DNA repair activity and facilitate 1,N6-ethenoadenine-induced mutations
    Sanjay Adhikari
    From the Molecular Oncology Program, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, D C 20057, Cancer Research Program, Houston Methodist Hospital Research Institute, Houston, Texas 77030, and
    J Biol Chem 290:4966-80. 2015
  9. pmc Expression, purification and characterization of codon-optimized human N-methylpurine-DNA glycosylase from Escherichia coli
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, LL level, S 122, 3800 Reservoir Road, NW, Washington, DC 20057, USA
    Protein Expr Purif 58:257-62. 2008
  10. pmc A comparative study of recombinant mouse and human apurinic/apyrimidinic endonuclease
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    Mol Cell Biochem 362:195-201. 2012

Collaborators

  • A Uren
  • Sivanesan Dakshanamurthy
  • Raja Mazumder
  • Sona Vasudevan
  • R Scott Williams
  • Eric Glasgow
  • Rabindra Roy
  • Jordan Woodrick
  • Soumendra Krishna Karmahapatra
  • Sujata Choudhury
  • Yasemin Saygideğer Kont
  • Monica Dixon
  • Suhani Gupta
  • Shyama Prasad Sajankila
  • Sankar Mitra
  • Priyanka Dhopeshwarkar
  • Bhaskar Kallakury
  • Apurva Mallisetty
  • Jeena Mathew
  • Christina Kraus
  • Arijit Dutta
  • Tsion Minas
  • Venkata M Yenugonda
  • Stephen Devito
  • Sanchita Sarangi
  • Akriti Trehan
  • Pooja Khatkar
  • Tapas Saha
  • Ganga Narasimhan
  • Keisuke Izumi
  • Praveen V Manthena
  • Amrita Cheema

Detail Information

Publications18

  1. ncbi request reprint Dipole-dipole interaction stabilizes the transition state of apurinic/apyrimidinic endonuclease--abasic site interaction
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, 3800 Reservoir Road NW, Washington, D C 20057, USA
    J Biol Chem 283:1334-9. 2008
    ..Thus, low activation energy and the enthalpy of activation, which are perhaps a result of dipole-dipole interactions, play critical roles in AP site binding of APE...
  2. pmc Evidence of complete cellular repair of 1,N6-ethenoadenine, a mutagenic and potential damage for human cancer, revealed by a novel method
    Sujata Choudhury
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    Mol Cell Biochem 313:19-28. 2008
    ..Furthermore, the epsilonA repair in vivo and in vitro is predominant in the G0/G1 phase of the cell cycle...
  3. pmc Characterization of magnesium requirement of human 5'-tyrosyl DNA phosphodiesterase mediated reaction
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    BMC Res Notes 5:134. 2012
    ..Although hTDP2 possesses both 3'- and 5'- tyrosyl DNA phosphodiesterase activity, the role of Mg2+ in its activity was not studied in sufficient details...
  4. pmc A unified method for purification of basic proteins
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    Anal Biochem 400:203-6. 2010
    ..Approximately, two-fifths of human proteome, including many if not all nucleic acid-interacting proteins, have a pI of 7.94 or higher; virtually all these 12,000 proteins may be purified using this method in a single step...
  5. pmc Development of a novel assay for human tyrosyl DNA phosphodiesterase 2
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    Anal Biochem 416:112-6. 2011
    ..Our results suggest that this novel assay system with this new TDP2 substrate can be used for inhibitor screening in a high-throughput manner...
  6. pmc Excised damaged base determines the turnover of human N-methylpurine-DNA glycosylase
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, United States
    DNA Repair (Amst) 8:1201-6. 2009
    ..Thus, the results provide the first evidence that the excised base rather than AP-site could be rate-limiting for DNA-glycosylase reactions...
  7. ncbi request reprint N-terminal extension of N-methylpurine DNA glycosylase is required for turnover in hypoxanthine excision reaction
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, D C 20057, USA
    J Biol Chem 282:30078-84. 2007
    ..The results from this study also affirm the need for reinvestigation of full-length MPG for its enzymatic and structural properties, which are currently available mostly for the truncated protein...
  8. pmc Germ line variants of human N-methylpurine DNA glycosylase show impaired DNA repair activity and facilitate 1,N6-ethenoadenine-induced mutations
    Sanjay Adhikari
    From the Molecular Oncology Program, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, D C 20057, Cancer Research Program, Houston Methodist Hospital Research Institute, Houston, Texas 77030, and
    J Biol Chem 290:4966-80. 2015
    ..Together our results suggest that individuals carrying R120C and R141Q MPG variants may be at risk for genomic instability and associated diseases as a consequence. ..
  9. pmc Expression, purification and characterization of codon-optimized human N-methylpurine-DNA glycosylase from Escherichia coli
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, LL level, S 122, 3800 Reservoir Road, NW, Washington, DC 20057, USA
    Protein Expr Purif 58:257-62. 2008
    ..Thus, this study indicates that this improved expression and purification system will facilitate large scale production and purification of a stable human MPG protein for further biochemical, biophysical and structure-function analysis...
  10. pmc A comparative study of recombinant mouse and human apurinic/apyrimidinic endonuclease
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    Mol Cell Biochem 362:195-201. 2012
    ..This includes the development and validation of effective APE1 inhibitors as chemosensitizers in clinical studies...
  11. ncbi request reprint Targeting base excision repair for chemosensitization
    Sanjay Adhikari
    Lombardi Comprehensive Cancer Center, 3800 Reservoir Road NW, Georgetown University Medical Center, Washington, DC 20057, USA
    Anticancer Agents Med Chem 8:351-7. 2008
    ..Thus, MPG and other BER proteins could be potential targets for chemosensitization...
  12. pmc Naturally occurring polyphenol, morin hydrate, inhibits enzymatic activity of N-methylpurine DNA glycosylase, a DNA repair enzyme with various roles in human disease
    Monica Dixon
    Department of Oncology, Georgetown Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, United States
    Bioorg Med Chem 23:1102-11. 2015
    ....
  13. pmc Redox regulation of apurinic/apyrimidinic endonuclease 1 activity in Long-Evans Cinnamon rats during spontaneous hepatitis
    Soumendra Krishna Karmahapatra
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical School, Georgetown University Medical Center, LL level, S 122 3800 Reservoir Road, NW, Washington, DC, 20057, USA
    Mol Cell Biochem 388:185-93. 2014
    ....
  14. pmc Suppression of tumor suppressor Tsc2 and DNA repair glycosylase Nth1 during spontaneous liver tumorigenesis in Long-Evans Cinnamon rats
    Shyama Prasad Sajankila
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical School, Washington, DC 20057, USA
    Mol Cell Biochem 338:233-9. 2010
    ..Increase in protein oxidation (carbonyl content) during the same time period (16-24 weeks) may have an effect on the promoter binding of regulatory proteins and consequent decrease in Nth1 and Tsc2 gene expressions during tumorigenesis...
  15. ncbi request reprint Magnesium, essential for base excision repair enzymes, inhibits substrate binding of N-methylpurine-DNA glycosylase
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    J Biol Chem 281:29525-32. 2006
    ....
  16. pmc Discrimination of lesion removal of N-methylpurine-DNA glycosylase revealed by a potent neutralizing monoclonal antibody
    Sanjay Adhikari
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, United States
    DNA Repair (Amst) 7:31-9. 2008
    ..Since we found that this antibody has an epitope in the N-terminal tail, the latter appears to have an important role in substrate discrimination, however, with a differential effect on different substrates...
  17. doi request reprint Depletion of tyrosyl DNA phosphodiesterase 2 activity enhances etoposide-mediated double-strand break formation and cell killing
    Yasemin Saygideğer Kont
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, United States Department of Molecular Medicine, Institute of Health Sciences, Dokuz Eylul University, Inciralti, Izmir 35340, Turkey Electronic address
    DNA Repair (Amst) 43:38-47. 2016
    ..These findings underscore the feasibility of clinical use of suitable TDP2 inhibitors in adjuvant therapy with Top2-poisons for a sub-set of cancer patients with high TDP2 expression. ..
  18. pmc Slow repair of lipid peroxidation-induced DNA damage at p53 mutation hotspots in human cells caused by low turnover of a DNA glycosylase
    Jordan Woodrick
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical School, Washington, DC 20057, USA
    Nucleic Acids Res 42:9033-46. 2014
    ....