Genomes and Genes
Affiliation: Fred Hutchinson Cancer Research Center
- Optimizing viable leukocyte sampling from the female genital tract for clinical trials: an international multi-site studyLyle R McKinnon
Department of Medicine, University of Toronto, Toronto, Canada Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya
PLoS ONE 9:e85675. 2014..However, the best female genital tract sampling technique is unclear...
- HIV infection of the genital mucosa in womenFlorian Hladik
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, D3 100, Seattle, WA 98109 1024, USA
Curr HIV/AIDS Rep 6:20-8. 2009..Studies using human explant tissues and in vivo animal studies have improved our understanding of the cellular and molecular pathways of infection; this information could be harnessed to design effective HIV vaccines and microbicides...
- Preventing mucosal HIV transmission with topical microbicides: challenges and opportunitiesFlorian Hladik
Department of Obstetrics and Gynecology, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, USA
Antiviral Res 88:S3-9. 2010..This article forms part of a special supplement on presentations covering HIV transmission and microbicides, based on the symposium "Trends in Microbicide Formulations", held on 25 and 26 January 2010, Arlington, VA...
- Atopic dermatitis with increased severity along a line of BlaschkoFlorian Hladik
Division of Immunology, Department of Dermatology, Allergy and Infectious Diseases, University of Vienna Medical School, Vienna, Austria
J Am Acad Dermatol 53:S221-4. 2005..Individuals such as our patient offer an opportunity to identify intraindividual genetic variations marking loci involved in the pathogenesis of atopy and atopic eczema...
- Repeat-region polymorphisms in the gene for the dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin-related molecule: effects on HIV-1 susceptibilityHuanliang Liu
Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, Washington 98195 8070, USA
J Infect Dis 193:698-702. 2006..5% in high-risk HIV-1-seronegative individuals vs. 27.6% in HIV-1-seropositive individuals; P=.0291). These findings suggest that DC-SIGNR polymorphisms may influence susceptibility to HIV-1...
- Persistence of HIV-1 receptor-positive cells after HSV-2 reactivation is a potential mechanism for increased HIV-1 acquisitionJia Zhu
Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
Nat Med 15:886-92. 2009..The persistence and enrichment of HIV receptor-positive inflammatory cells in the genitalia help explain the inability of anti-HSV-2 therapy to reduce HIV acquisition...
- Ex vivo comparison of microbicide efficacies for preventing HIV-1 genomic integration in intraepithelial vaginal cellsM Juliana McElrath
Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Antimicrob Agents Chemother 54:763-72. 2010..8 microg/ml). These results highlight the merit of our model for screening the mucosal efficacies of novel microbicides and their formulations and potentially rank ordering candidates for clinical evaluation...
- Vaginal langerhans cells nonproductively transporting HIV-1 mediate infection of T cellsLamar Ballweber
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, D3 100, Seattle, WA 98109 1024, USA
J Virol 85:13443-7. 2011..Transport by migratory LCs to local lymphatics in a nonproductive but infectious form may aid HIV-1 in evasion of topical microbicides that target its intracellular productive life cycle...
- Combined effect of CCR5-Delta32 heterozygosity and the CCR5 promoter polymorphism -2459 A/G on CCR5 expression and resistance to human immunodeficiency virus type 1 transmissionFlorian Hladik
Fred Hutchinson Cancer Research Center, Clinical Research Division, Program in Infectious Diseases, 1100 Fairview Ave N, P O Box 19024, D3 100, Seattle, WA 98109 1024, USA
J Virol 79:11677-84. 2005..We conclude that the CCR5 ORF delta32/wt-CCR5 -2459 A/G genotype combination offers an advantage in resisting sexual HIV-1 transmission and that this effect is mediated by a relative paucity of CCR5 on potential target cells of HIV-1...
- A study of the osmotic characteristics, water permeability, and cryoprotectant permeability of human vaginal immune cellsZhiquan Shu
Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA School of Mechanical and Materials Engineering, Washington State University, Everett, WA 98201, USA
Cryobiology 72:93-9. 2016..Thus, DMSO, PG, and EG are better options than glycerol in avoiding severe cell volume excursion and osmotic injury during CPA addition and removal for cryopreservation of human vaginal immune cells. ..
- Initial events in establishing vaginal entry and infection by human immunodeficiency virus type-1Florian Hladik
Program in Infectious Diseases, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Immunity 26:257-70. 2007..Our findings shed light on the very earliest steps of mucosal HIV infection in vivo and may guide the design of effective strategies to block local transmission and prevent HIV-1 spread...
- Impaired viral entry cannot explain reduced CD4+ T cell susceptibility to HIV type 1 in certain highly exposed individualsEmily C Speelmon
Medical Scientist Training Program, University of Washington, Seattle, Washington 98105, USA
AIDS Res Hum Retroviruses 24:1415-27. 2008..Identifying the host-virus interactions responsible for diminished in vitro susceptibility may contribute to the development of novel therapeutic strategies...
- Virus-specific CD8+ T cells accumulate near sensory nerve endings in genital skin during subclinical HSV-2 reactivationJia Zhu
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
J Exp Med 204:595-603. 2007..These findings indicate that the frequency and clinical course of HSV-2 reactivation in humans is influenced by virus-specific CD8(+) T cells that persist in peripheral mucosa and genital skin after resolution of herpes lesions...
- Cryopreservation of Human Mucosal LeukocytesSean M Hughes
Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington, United States of America
PLoS ONE 11:e0156293. 2016..An effective cryopreservation protocol for these cells following their isolation will make studying them more feasible...
- Most DC-SIGNR transcripts at mucosal HIV transmission sites are alternatively spliced isoformsHuanliang Liu
Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA
Eur J Hum Genet 13:707-15. 2005..Soluble isoforms, in particular, may modulate the efficiency of viral transmission and dissemination...
- Comprehensive assessment of HIV target cells in the distal human gut suggests increasing HIV susceptibility toward the anusM J McElrath
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
J Acquir Immune Defic Syndr 63:263-71. 2013..Yet, little is known about the specific target-cell milieu in the human rectum other than inferences made from the colon...
- Vpu-deficient HIV strains stimulate innate immune signaling responses in target cellsBrian P Doehle
University of Washington School of Medicine, Department of Immunology, Seattle, Washington, USA
J Virol 86:8499-506. 2012..These observations show that HIV-1 can indeed be recognized as a pathogen in infected cells and provide a novel and effective platform for defining the native innate immune programs of target cells of HIV-1 infection...
- Human immunodeficiency virus type 1 mediates global disruption of innate antiviral signaling and immune defenses within infected cellsBrian P Doehle
University of Washington School of Medicine, Department of Immunology, Seattle, WA 98195, USA
J Virol 83:10395-405. 2009..IRF-3 may direct an innate antiviral response that regulates HIV-1 replication and viral set point while governing susceptibility to opportunistic virus coinfections...
- Determination of the Membrane Permeability to Water of Human Vaginal Mucosal Immune Cells at Subzero Temperatures Using Differential Scanning CalorimetryZhiquan Shu
1 Department of Mechanical Engineering, University of Washington, Seattle, Washington
Biopreserv Biobank 14:307-13. 2016..Theoretical simulations predicted that the optimal cooling rate for both T cells and macrophages was about -3°C/min, which was proven by preliminary immune cell cryopreservation experiments. ..
- Setting the stage: host invasion by HIVFlorian Hladik
Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Nat Rev Immunol 8:447-57. 2008..Furthermore, we discuss the gaps in our knowledge that are relevant to future studies, which will shape strategies for effective HIV prevention...
- Most highly exposed seronegative men lack HIV-1-specific, IFN-gamma-secreting T cellsFlorian Hladik
Program in Infectious Diseases, Clinical Research Division, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, D3 100, Seattle, WA 98109, USA
J Immunol 171:2671-83. 2003..HIV-1-specific, IFN-gamma-secreting T cells are thus unlikely to substantially contribute to resistance against infection in most exposed seronegative men having sex with men...
- Mucosal effects of tenofovir 1% gelFlorian Hladik
Department of Obstetrics and Gynecology, University of Washington, Seattle, United States
elife 4:. 2015..The breadth of mucosal changes induced by tenofovir indicates that its safety over longer-term topical use should be carefully monitored. ..
- Mechanisms of Sexual HIV-1 TransmissionFlorian Hladik; Fiscal Year: 2006..These studies will improve our knowledge of the human genital immune system and shed light on the mechanisms of HIV-l targeting and strain selection in the female genital tract. ..
- Mechanisms of HIV-1 Transmission in the Genital Mucosa of WomenFlorian Hladik; Fiscal Year: 2008..In this proposal, we will use a novel organ culture model of human vaginal tissue obtained from surgeries to characterize the entry pathways of HIV during the earliest stages of sexual transmission. [unreadable] [unreadable] [unreadable]..