Roberto De Pascalis

Summary

Affiliation: Food and Drug Administration
Country: USA

Publications

  1. pmc Activities of Murine Peripheral Blood Lymphocytes Provide Immune Correlates That Predict Francisella tularensis Vaccine Efficacy
    Roberto De Pascalis
    Laboratory of Mucosal Pathogens and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Silver Spring, Maryland, USA
    Infect Immun 84:1054-61. 2016
  2. pmc Diverse myeloid and lymphoid cell subpopulations produce gamma interferon during early innate immune responses to Francisella tularensis live vaccine strain
    Roberto De Pascalis
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Research and Evaluation, US FDA, 1401 Rockville Pike, HFM 431, Rockville, Maryland 20852, USA
    Infect Immun 76:4311-21. 2008
  3. pmc Development of functional and molecular correlates of vaccine-induced protection for a model intracellular pathogen, F. tularensis LVS
    Roberto De Pascalis
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Rockville, Maryland, United States of America
    PLoS Pathog 8:e1002494. 2012
  4. doi request reprint NK cells activated in vivo by bacterial DNA control the intracellular growth of Francisella tularensis LVS
    Karen L Elkins
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic, and Allergenic Products, CBER FDA, 1401 Rockville Pike, HFM 431, Rockville, MD 20852, USA
    Microbes Infect 11:49-56. 2009
  5. pmc T cells from lungs and livers of Francisella tularensis-immune mice control the growth of intracellular bacteria
    Carmen M Collazo
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Rockville, Maryland 20852, USA
    Infect Immun 77:2010-21. 2009
  6. pmc Francisella tularensis Vaccines Elicit Concurrent Protective T- and B-Cell Immune Responses in BALB/cByJ Mice
    Roberto De Pascalis
    Laboratory of Mucosal Pathogens and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Silver Spring, MD, United States of America
    PLoS ONE 10:e0126570. 2015
  7. pmc Epicutaneous model of community-acquired Staphylococcus aureus skin infections
    Ranjani Prabhakara
    Center for Biologics Evaluation and Research, FDA, Bethesda, Maryland, USA
    Infect Immun 81:1306-15. 2013
  8. doi request reprint Progress, challenges, and opportunities in Francisella vaccine development
    Karen L Elkins
    a Division of Bacterial, Parasitic, and Allergenic Products, CBER FDA, Silver Spring, MD, USA
    Expert Rev Vaccines 15:1183-96. 2016
  9. doi request reprint GM-CSF has disparate roles during intranasal and intradermal Francisella tularensis infection
    Sherry L Kurtz
    Laboratory of Mucosal Pathogens and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Silver Spring, MD 20903, USA
    Microbes Infect . 2016
  10. pmc Analysis of the Fc gamma receptor-dependent component of neutralization measured by anthrax toxin neutralization assays
    Anita Verma
    Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    Clin Vaccine Immunol 16:1405-12. 2009

Collaborators

Detail Information

Publications14

  1. pmc Activities of Murine Peripheral Blood Lymphocytes Provide Immune Correlates That Predict Francisella tularensis Vaccine Efficacy
    Roberto De Pascalis
    Laboratory of Mucosal Pathogens and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Silver Spring, Maryland, USA
    Infect Immun 84:1054-61. 2016
    ..tularensis. Our studies demonstrate that murine PBLs can be used productively to identify potential correlates of protection against F. tularensis and to expand and refine a comprehensive set of protective correlates...
  2. pmc Diverse myeloid and lymphoid cell subpopulations produce gamma interferon during early innate immune responses to Francisella tularensis live vaccine strain
    Roberto De Pascalis
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Research and Evaluation, US FDA, 1401 Rockville Pike, HFM 431, Rockville, Maryland 20852, USA
    Infect Immun 76:4311-21. 2008
    ....
  3. pmc Development of functional and molecular correlates of vaccine-induced protection for a model intracellular pathogen, F. tularensis LVS
    Roberto De Pascalis
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Rockville, Maryland, United States of America
    PLoS Pathog 8:e1002494. 2012
    ..The overall approach may be applicable to intracellular pathogens in general...
  4. doi request reprint NK cells activated in vivo by bacterial DNA control the intracellular growth of Francisella tularensis LVS
    Karen L Elkins
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic, and Allergenic Products, CBER FDA, 1401 Rockville Pike, HFM 431, Rockville, MD 20852, USA
    Microbes Infect 11:49-56. 2009
    ..Thus, NK cells contribute to DNA-mediated protection by production of cytokines including IFN-gamma and TNF-alpha, resulting in nitric oxide production and control of intracellular Francisella replication...
  5. pmc T cells from lungs and livers of Francisella tularensis-immune mice control the growth of intracellular bacteria
    Carmen M Collazo
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Rockville, Maryland 20852, USA
    Infect Immun 77:2010-21. 2009
    ..Thus, our results indicate functional similarities between immune T cells residing in spleens, livers, and lungs of LVS-immune mice...
  6. pmc Francisella tularensis Vaccines Elicit Concurrent Protective T- and B-Cell Immune Responses in BALB/cByJ Mice
    Roberto De Pascalis
    Laboratory of Mucosal Pathogens and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Silver Spring, MD, United States of America
    PLoS ONE 10:e0126570. 2015
    ..tularensis, and illustrate the complexity necessary to define a comprehensive set of correlates. ..
  7. pmc Epicutaneous model of community-acquired Staphylococcus aureus skin infections
    Ranjani Prabhakara
    Center for Biologics Evaluation and Research, FDA, Bethesda, Maryland, USA
    Infect Immun 81:1306-15. 2013
    ..In vivo neutrophil depletion demonstrated that neutrophils play a protective role in preventing bacterial dissemination and fatal invasive infection...
  8. doi request reprint Progress, challenges, and opportunities in Francisella vaccine development
    Karen L Elkins
    a Division of Bacterial, Parasitic, and Allergenic Products, CBER FDA, Silver Spring, MD, USA
    Expert Rev Vaccines 15:1183-96. 2016
    ..Because field trials of new vaccines will be difficult to design, using such measurements to derive potential correlates of protection may be important to bridge between animal efficacy studies and people. ..
  9. doi request reprint GM-CSF has disparate roles during intranasal and intradermal Francisella tularensis infection
    Sherry L Kurtz
    Laboratory of Mucosal Pathogens and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Silver Spring, MD 20903, USA
    Microbes Infect . 2016
    ..Taken together, our data demonstrates that the absence of GM-CSF improves resistance to pulmonary, but not intradermal, infection with Francisella...
  10. pmc Analysis of the Fc gamma receptor-dependent component of neutralization measured by anthrax toxin neutralization assays
    Anita Verma
    Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    Clin Vaccine Immunol 16:1405-12. 2009
    ..These findings should be considered when interpreting anthrax toxin neutralization assay output...
  11. ncbi request reprint Inhibition of CD4(+)25+ T regulatory cell function implicated in enhanced immune response by low-dose cyclophosphamide
    M E Christine Lutsiak
    Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bldg 10, Rm 8B09, Bethesda, MD 20892, USA
    Blood 105:2862-8. 2005
    ..The relevance of the loss of suppressor functionality and the changes in gene expression are further discussed...
  12. ncbi request reprint SDR grafting of a murine antibody using multiple human germline templates to minimize its immunogenicity
    Noreen R Gonzales
    Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, Room 8B09, 10 Center Drive, Building 10, Bethesda, MD 20892 1750, USA
    Mol Immunol 41:863-72. 2004
    ....
  13. ncbi request reprint Grafting of "abbreviated" complementarity-determining regions containing specificity-determining residues essential for ligand contact to engineer a less immunogenic humanized monoclonal antibody
    Roberto De Pascalis
    Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 169:3076-84. 2002
    ..The final variant of the HuCOL-1, which retains its Ag-binding reactivity and shows significantly lower serum reactivity than that of the parental Ab, can serve as a prototype for the development of a potentially useful clinical reagent...