Abdu I Alayash

Summary

Affiliation: Food and Drug Administration
Country: USA

Publications

  1. doi Setbacks in blood substitutes research and development: a biochemical perspective
    Abdu I Alayash
    Division of Hematology, Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration PHS, 8800 Rockville Pike, Bethesda, MD 20892, USA
    Clin Lab Med 30:381-9. 2010
  2. ncbi First-generation blood substitutes: what have we learned? Biochemical and physiological perspectives
    Abdu I Alayash
    Center for Biologics Evaluation and Research, Food and Drug Administration, Laboratory of Biochemistry and Vascular Biology, Division of Hematology, National Institutes of Health Campus, Bethesda, MD 20892, USA
    Expert Opin Biol Ther 7:665-75. 2007
  3. doi Hemoglobin-Based Blood Substitutes and the Treatment of Sickle Cell Disease: More Harm than Help?
    Abdu I Alayash
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20993, USA
    Biomolecules 7:. 2017
  4. pmc Effects of carbon monoxide (CO) delivery by a CO donor or hemoglobin on vascular hypoxia inducible factor 1α and mitochondrial respiration
    Chad E N Reiter
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research CBER, U S Food and Drug Administration FDA, Bethesda, MD 20892, United States
    FEBS Open Bio 2:113-8. 2012
  5. pmc Blood substitutes: why haven't we been more successful?
    Abdu I Alayash
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Office of Blood Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA Electronic address
    Trends Biotechnol 32:177-85. 2014
  6. doi Haptoglobin: old protein with new functions
    Abdu I Alayash
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, MD 20892, USA
    Clin Chim Acta 412:493-8. 2011
  7. doi Haptoglobin: the hemoglobin detoxifier in plasma
    Abdu I Alayash
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA Electronic address
    Trends Biotechnol 31:2-3. 2013
  8. doi Haptoglobin preserves the CD163 hemoglobin scavenger pathway by shielding hemoglobin from peroxidative modification
    Paul W Buehler
    Center for Biologics Evaluation and Research CBER, US Food and Drug Administration FDA, Washington, DC, USA
    Blood 113:2578-86. 2009
  9. doi Induction of hypoxia inducible factor (HIF-1α) in rat kidneys by iron chelation with the hydroxypyridinone, CP94
    Jin Hyen Baek
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1809:262-8. 2011
  10. pmc Haptoglobin preferentially binds β but not α subunits cross-linked hemoglobin tetramers with minimal effects on ligand and redox reactions
    Yiping Jia
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, United States of America
    PLoS ONE 8:e59841. 2013

Collaborators

Detail Information

Publications56

  1. doi Setbacks in blood substitutes research and development: a biochemical perspective
    Abdu I Alayash
    Division of Hematology, Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration PHS, 8800 Rockville Pike, Bethesda, MD 20892, USA
    Clin Lab Med 30:381-9. 2010
    ..It is hoped that this will aid in the development of a safe and effective second generation of HBOCs...
  2. ncbi First-generation blood substitutes: what have we learned? Biochemical and physiological perspectives
    Abdu I Alayash
    Center for Biologics Evaluation and Research, Food and Drug Administration, Laboratory of Biochemistry and Vascular Biology, Division of Hematology, National Institutes of Health Campus, Bethesda, MD 20892, USA
    Expert Opin Biol Ther 7:665-75. 2007
    ..Here the authors provide an overview of their research experiences, novel insights into the molecular basis of toxicities of these products and some lessons learned...
  3. doi Hemoglobin-Based Blood Substitutes and the Treatment of Sickle Cell Disease: More Harm than Help?
    Abdu I Alayash
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20993, USA
    Biomolecules 7:. 2017
    ..This review discusses the advantages and disadvantages of using HBOCs in SCD...
  4. pmc Effects of carbon monoxide (CO) delivery by a CO donor or hemoglobin on vascular hypoxia inducible factor 1α and mitochondrial respiration
    Chad E N Reiter
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research CBER, U S Food and Drug Administration FDA, Bethesda, MD 20892, United States
    FEBS Open Bio 2:113-8. 2012
    ..Together, CO reduced ET-1, and, at low doses, had no effect on endothelial mitochondria oxygen consumption. CO ligation to Hb may be developed further as non-vasoactive oxygen therapeutic without compromising mitochondrial function...
  5. pmc Blood substitutes: why haven't we been more successful?
    Abdu I Alayash
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Office of Blood Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA Electronic address
    Trends Biotechnol 32:177-85. 2014
    ..Moreover, recent mechanistic and animal studies support a role for globin and heme scavengers in controlling oxidative toxicity associated with Hb infusion. ..
  6. doi Haptoglobin: old protein with new functions
    Abdu I Alayash
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, MD 20892, USA
    Clin Chim Acta 412:493-8. 2011
    ..It may prove necessary to explore these protective clearing mechanisms to counter the toxicity associated with free Hb when used as oxygen therapeutics in hemolytic anemias and in RBC storage lesions...
  7. doi Haptoglobin: the hemoglobin detoxifier in plasma
    Abdu I Alayash
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA Electronic address
    Trends Biotechnol 31:2-3. 2013
    ..Potential therapeutic benefits of using Hp for inactivation and clearance of free Hb under a number of clinical settings are considered...
  8. doi Haptoglobin preserves the CD163 hemoglobin scavenger pathway by shielding hemoglobin from peroxidative modification
    Paul W Buehler
    Center for Biologics Evaluation and Research CBER, US Food and Drug Administration FDA, Washington, DC, USA
    Blood 113:2578-86. 2009
    ..In addition, our data provide in vivo evidence that unbound Hb is oxidatively modified within extravascular compartments consistent with our in vitro findings...
  9. doi Induction of hypoxia inducible factor (HIF-1α) in rat kidneys by iron chelation with the hydroxypyridinone, CP94
    Jin Hyen Baek
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1809:262-8. 2011
    ..In conclusion, we have identified the inhibition of iron-binding pocket of PHD as an underlying mechanism of HIF induction in vivo and in vitro by a bidentate hydroxypyridinone...
  10. pmc Haptoglobin preferentially binds β but not α subunits cross-linked hemoglobin tetramers with minimal effects on ligand and redox reactions
    Yiping Jia
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, United States of America
    PLoS ONE 8:e59841. 2013
    ..This may ultimately provide a safe oxidative inactivation and clearance pathway for chemically modified Hbs in circulation...
  11. ncbi Effects of endogenous ascorbate on oxidation, oxygenation, and toxicokinetics of cell-free modified hemoglobin after exchange transfusion in rat and guinea pig
    Paul W Buehler
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    J Pharmacol Exp Ther 323:49-60. 2007
    ..The present findings suggest the importance of plasma AA in maintaining the stability of acellular Hb susceptible to oxidation, and they may be relevant to humans, which display a similar plasma/tissue antioxidant status to guinea pig...
  12. doi Effects of cross-linking and zero-link polymerization on oxygen transport and redox chemistry of bovine hemoglobin
    Yiping Jia
    Laboratory of Biochemistry and Vascular Biology LBVB, Division of Hematology, Center for Biologics Evaluation and Research CBER, Food and Drug Administration FDA, MD 20892, USA
    Biochim Biophys Acta 1794:1234-42. 2009
    ....
  13. doi Isolated Hb Providence β82Asn and β82Asp fractions are more stable than native HbA(0) under oxidative stress conditions
    Bindu Abraham
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, Maryland 20892, United States
    Biochemistry 50:9752-66. 2011
    ....
  14. ncbi Oxygen binding and oxidation reactions of human hemoglobin conjugated to carboxylate dextran
    Yiping Jia
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1672:164-73. 2004
    ..However, the reduction in the allosteric function of this protein and the lack of apparent quaternary T-->R transition may hinder its physiological role as an oxygen transporter...
  15. ncbi Structural basis of peroxide-mediated changes in human hemoglobin: a novel oxidative pathway
    Yiping Jia
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    J Biol Chem 282:4894-907. 2007
    ....
  16. ncbi Structural and functional characterization of glutaraldehyde-polymerized bovine hemoglobin and its isolated fractions
    Paul W Buehler
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research CBER, Food and Drug Administration FDA, Bethesda, Maryland 20892, USA
    Anal Chem 77:3466-78. 2005
    ..Structural modification imparted by glutaraldehyde resulted in nearly identical functional characteristics of PolyHbBv and its fractions with regard to oxygen equilibrium, ligand binding, and autoxidative kinetics...
  17. ncbi Oxygen sensing in the circulation: "cross talk" between red blood cells and the vasculature
    Paul W Buehler
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Bethesda, MD 20892, USA
    Antioxid Redox Signal 6:1000-10. 2004
    ..We believe that there are important and yet unexplored mechanisms by which RBCs can directly or indirectly communicate via redox intermediates with extravascular sites as part of the global O(2) sensing mechanism...
  18. pmc Redox properties of human hemoglobin in complex with fractionated dimeric and polymeric human haptoglobin
    Todd L Mollan
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20852, USA
    Free Radic Biol Med 69:265-77. 2014
    ....
  19. doi Inactivation of prolyl hydroxylase domain (PHD) protein by epigallocatechin (EGCG) stabilizes hypoxia-inducible factor (HIF-1α) and induces hepcidin (Hamp) in rat kidney
    Dominador J Manalo
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Biochem Biophys Res Commun 416:421-6. 2011
    ..These data demonstrate EGCG's therapeutic potential in modulating hepcidin expression in diseases associated with altered iron metabolism...
  20. ncbi O-raffinose crosslinked hemoglobin lacks site-specific chemistry in the central cavity: structural and functional consequences of beta93Cys modification
    Robert A Boykins
    Laboratory of Biophysics, Division of Bacterial, Parasitic and Allergenic Products, Bethesda, Maryland 20892, USA
    Proteins 59:840-55. 2005
    ..Structural data presented here when taken together with the oxidative instability of O-R-PolyHbA0 may provide some basis for the reported toxicity of this oxygen carrier...
  21. ncbi Effects of cell-free hemoglobin on hypoxia-inducible factor (HIF-1alpha) and heme oxygenase (HO-1) expressions in endothelial cells subjected to hypoxia
    Li Hong Yeh
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Antioxid Redox Signal 6:944-53. 2004
    ..DBBF-Hb modulates key cell-signaling pathways by competing with peroxides required for the deactivation of HIF-1alpha, which may modulate important physiological mediators...
  22. pmc Familial secondary erythrocytosis due to increased oxygen affinity is caused by destabilization of the T state of hemoglobin Brigham (α₂β₂(Pro100Leu))
    Todd L Mollan
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    Protein Sci 21:1444-55. 2012
    ..These kinetic data help explain the high O₂ affinity characteristics of Hb Brigham and provide further evidence for the importance of the contribution of Pro100 to intersubunit contacts and stabilization of the T quaternary structure...
  23. doi Heme binding to human alpha-1 proteinase inhibitor
    Elena Karnaukhova
    Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1820:2020-9. 2012
    ..While heme binding by hemopexin, albumin and α(1)-microglobulin has been extensively studied, the role of other plasma proteins remains largely unknown...
  24. pmc Cross-linking with O-raffinose lowers oxygen affinity and stabilizes haemoglobin in a non-cooperative T-state conformation
    Yiping Jia
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research CBER, Food and Drug Administration FDA, Bethesda, Maryland 20892, USA
    Biochem J 384:367-75. 2004
    ..Although the physiological ramifications of locking HbA(0) in the T conformation with the O-raffinose are still unknown, valuable insights into haemoglobin function are provided by these studies of O-R-polyHbA(0)...
  25. doi Acellular haemoglobin attenuates hypoxia-inducible factor-1alpha (HIF-1alpha) and its target genes in haemodiluted rats
    Dominador J Manalo
    LBVB Laboratory of Biochemistry and Vascular Biology, Division of Hematology, CBER Center for Biologics Evaluation and Research, FDA Food and Drug Administration, NIH National Institutes of Health, Bethesda, MD 20892, USA
    Biochem J 414:461-9. 2008
    ....
  26. doi Structural stabilization in tetrameric or polymeric hemoglobin determines its interaction with endogenous antioxidant scavenger pathways
    Paul W Buehler
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research CBER, U S Food and Drug Administration FDA, Rockville, Maryland, USA
    Antioxid Redox Signal 10:1449-62. 2008
    ..Based on these results, a rational and systematic approach to HBOC design may be used to optimize interaction with endogenous Hb clearance and detoxification pathways...
  27. pmc α-Hemoglobin stabilizing protein (AHSP) markedly decreases the redox potential and reactivity of α-subunits of human HbA with hydrogen peroxide
    Todd L Mollan
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20852, USA
    J Biol Chem 288:4288-98. 2013
    ..Hexacoordination in the AHSP·met-α complex markedly decreases the rate of the initial H(2)O(2) reaction with iron and thus provides α-subunits protection against damaging oxidative reactions...
  28. doi All hemoglobin-based oxygen carriers are not created equally
    Paul W Buehler
    Laboratory of Biochemistry and Vascular Biology LBVB, Division of Hematology, Center for Biologics Evaluation and Research CBER, Food and Drug Administration FDA, Maryland 20892, USA
    Biochim Biophys Acta 1784:1378-81. 2008
    ....
  29. doi Effects of (-)-epigallocatechin gallate on the redox reactions of human hemoglobin
    Yiping Jia
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, National Institutes of Health Campus, Bethesda, MD 20892, USA
    Free Radic Biol Med 45:659-66. 2008
    ..A balance between pro and antioxidant properties of EGCG should be taken into account if EGCG is used in combination therapy with redox active acellular Hbs...
  30. ncbi Oxygen therapeutics: can we tame haemoglobin?
    Abdu I Alayash
    Laboratory of Biochemistry, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, 8800 Rockville Pike, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Rev Drug Discov 3:152-9. 2004
    ..Current protective strategies designed to produce safe Hb-based products are focused on controlling or suppressing the 'radical' nature of Hb while retaining its oxygen-carrying function...
  31. ncbi A role for the myoglobin redox cycle in the induction of endothelial cell apoptosis
    FELICE D'AGNILLO
    Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Free Radic Biol Med 33:1153-64. 2002
    ..These results suggest a role for the Mb redox cycle in the induction of endothelial cell apoptosis, which may be relevant in the pathophysiology of diseases characterized by the release of Mb from damaged muscle...
  32. ncbi Toxicities of hemoglobin solutions: in search of in-vitro and in-vivo model systems
    Paul W Buehler
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Bethesda, Maryland, USA
    Transfusion 44:1516-30. 2004
    ....
  33. pmc Sickle Cell Hemoglobin in the Ferryl State Promotes βCys-93 Oxidation and Mitochondrial Dysfunction in Epithelial Lung Cells (E10)
    Tigist Kassa
    From the Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland 20993 and
    J Biol Chem 290:27939-58. 2015
    ..Thus, highly oxidizing ferryl Hb and heme, the product of oxidation, may be central to the evolution of vasculopathy in SCD and may suggest therapeutic modalities that interrupt heme-mediated inflammation. ..
  34. pmc Post-translational transformation of methionine to aspartate is catalyzed by heme iron and driven by peroxide: a novel subunit-specific mechanism in hemoglobin
    Michael Brad Strader
    From the Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892
    J Biol Chem 289:22342-57. 2014
    ....
  35. doi Blood aging, safety, and transfusion: capturing the "radical" menace
    Paul W Buehler
    Division of Hematology, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Bethesda, Maryland 20892, USA
    Antioxid Redox Signal 14:1713-28. 2011
    ..Furthermore, some of the same naturally occurring protective mechanisms can potentially be employed to oxidatively inactivate this redox active protein and control its damaging side reactions when released outside of the RBC...
  36. ncbi Chemical characterization of diaspirin cross-linked hemoglobin polymerized with poly(ethylene glycol)
    Paul W Buehler
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research CBER, Food and Drug Administration FDA, Bethesda, Maryland 20892, USA
    Anal Chem 78:4634-41. 2006
    ..e., 64 kDa) has a direct influence on HBOC-mediated vasoactivity and that other protective strategies should be considered to control blood pressure imbalances...
  37. ncbi Redox biology of blood revisited: the role of red blood cells in maintaining circulatory reductive capacity
    Paul W Buehler
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Antioxid Redox Signal 7:1755-60. 2005
    ..In the current short review, we have revisited the topic of redox biology of blood and focused on yet another emerging area of research, which deals with the reductive power of blood and the physiological Redox Signal...
  38. pmc Oxidative instability of hemoglobin E (β26 Glu→Lys) is increased in the presence of free α subunits and reversed by α-hemoglobin stabilizing protein (AHSP): Relevance to HbE/β-thalassemia
    Michael Brad Strader
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA
    Redox Biol 8:363-74. 2016
    ....
  39. pmc Evaluation of Stem Cell-Derived Red Blood Cells as a Transfusion Product Using a Novel Animal Model
    Sandeep N Shah
    Laboratory of Cellular Hematology, Division of Hematology Research and Review, Office of Blood Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, United States of America
    PLoS ONE 11:e0166657. 2016
    ..While certain key differences remain between donor-derived RBCs and stemRBCs, the ability of stemRBCs to deliver oxygen in a living organism provides support for further development as a transfusion product...
  40. pmc Oxidized Ferric and Ferryl Forms of Hemoglobin Trigger Mitochondrial Dysfunction and Injury in Alveolar Type I Cells
    Narendranath Reddy Chintagari
    Laboratory of Biochemistry and Vascular Biology, Division of Hematology Review and Research, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland
    Am J Respir Cell Mol Biol 55:288-98. 2016
    ..Collectively, these data establish, for the first time, a central role for cell-free Hb in lung epithelial injury, and that these effects are mediated through the redox transition of Hb to higher oxidation states. ..
  41. ncbi Exploring Oxidative Reactions in Hemoglobin Variants Using Mass Spectrometry: Lessons for Engineering Oxidatively Stable Oxygen Therapeutics
    Michael Brad Strader
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland
    Antioxid Redox Signal . 2016
    ....
  42. pmc Determination of extinction coefficients of human hemoglobin in various redox states
    Fantao Meng
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, United States
    Anal Biochem . 2017
    ....
  43. pmc Haptoglobin attenuates hemoglobin-induced heme oxygenase-1 in renal proximal tubule cells and kidneys of a mouse model of sickle cell disease
    Narendranath Reddy Chintagari
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA
    Blood Cells Mol Dis 54:302-6. 2015
    ..Our results suggest that Hb-mediated oxidative toxicity may contribute to renal damage in SCD and that Hp treatment reduces heme/iron toxicity in the kidneys following hemolysis...
  44. pmc Redox reactions of hemoglobin: mechanisms of toxicity and control
    Todd L Mollan
    Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland
    Antioxid Redox Signal 18:2251-3. 2013
    ..Our goal has been to update this critically important research area, because we believe that it will ultimately impact the practice of transfusion medicine in a number of important ways. Antioxid. Redox Signal. 18, 2251-2253...
  45. ncbi Stopped-flow fluorescence method for the detection of heme degradation products in solutions of chemically modified hemoglobins and peroxide
    Yiping Jia
    Center for Biologics Evaluation and Research CBER, Food and Drug Administration FDA, Bethesda, Maryland 20892, USA
    Anal Biochem 308:186-8. 2002
  46. ncbi The heme pocket geometry of Lucina pectinata hemoglobin II restricts nitric oxide and peroxide entry: model of ligand control for the design of a stable oxygen carrier
    Walleska De Jesus-Bonilla
    Department of Chemistry, University of Puerto Rico, Mayaguez Campus, P O Box 9019, Mayagüez 00681 9019, Puerto Rico
    Biochemistry 46:10451-60. 2007
    ..Engineering of second-generation Hb-based oxygen therapeutics that are resistant to NO/H2O2-driven oxidation may ultimately require further optimization of the heme pocket architecture to limit heme exposure to solvent...
  47. ncbi Allosteric effects on oxidative and nitrosative reactions of cell-free hemoglobins
    Celia Bonaventura
    Nicholas School of the Environment and Earth Sciences, Duke University Marine Laboratory, Beaufort, North Carolina 28516, USA
    IUBMB Life 59:498-505. 2007
    ..These redox reactions can drive formation of SNO-Hb and other reactive species and are of significance for the use of cell-free Hbs in vivo...
  48. pmc Ascorbate removes key precursors to oxidative damage by cell-free haemoglobin in vitro and in vivo
    Jacqueline Dunne
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK
    Biochem J 399:513-24. 2006
    ..g. haemolytic anaemias, subarachnoid haemorrhage, rhabdomyolysis...
  49. ncbi CD163 is the macrophage scavenger receptor for native and chemically modified hemoglobins in the absence of haptoglobin
    Dominik J Schaer
    Medical Clinic B Research Unit, University Hospital, CH 8091 Zurich, Switzerland
    Blood 107:373-80. 2006
    ..These results identify CD163 as a scavenger receptor for native Hb and small-molecular-weight Hb-based blood substitutes after Hp depletion...
  50. ncbi Differential effects of sodium selenite in reducing tissue damage caused by three hemoglobin-based oxygen carriers
    Ann L Baldwin
    Department of Physiology, College of Medicine, University of Arizona, Tucson, Arizona 85724 5051, USA
    J Appl Physiol (1985) 96:893-903. 2004
    ....
  51. ncbi Sodium selenite reduces hemoglobin-induced venular leakage in the rat mesentery
    Ann L Baldwin
    Department of Physiology, College of Medicine, University of Arizona, Tucson 85724 5051, USA
    Am J Physiol Heart Circ Physiol 284:H81-91. 2003
    ..In vitro, Na(2)SeO(3) reduced the oxidation rate of DBBF-Hb while in the presence of oxidants. These results suggest that Na(2)SeO(3) reduces DBBF-Hb-induced microvascular leakage partly by retarding the oxidation of its heme iron...
  52. ncbi Comparison of effects of two hemoglobin-based O(2) carriers on intestinal integrity and microvascular leakage
    Ann L Baldwin
    Department of Physiology, College of Medicine, University of Arizona, Tucson 85724 5051, USA
    Am J Physiol Heart Circ Physiol 283:H1292-301. 2002
    ..These results indicate that intravascular PolyHbBv produces significantly less disruption of the intestinal exchange barrier than does DBBF-Hb, probably because the heme is not so easily oxidized...
  53. ncbi Site-specific cross-linking of human and bovine hemoglobins differentially alters oxygen binding and redox side reactions producing rhombic heme and heme degradation
    Enika Nagababu
    Molecular Dynamics Section, National Institute on Aging NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224 6823, USA
    Biochemistry 41:7407-15. 2002
    ..These findings establish the importance of these secondary oxidative reactions over autoxidation in evaluating the toxicity of HBOCs...
  54. ncbi Gating the radical hemoglobin to macrophages: the anti-inflammatory role of CD163, a scavenger receptor
    Dominik J Schaer
    Medical Clinic B Research Unit, University of Zurich, Switzerland
    Antioxid Redox Signal 9:991-9. 2007
    ..Here, an overview and novel insights into the role of CD163 in Hb redox inactivation and clearance are provided...
  55. ncbi Redox biology of blood
    Abdu I Alayash
    Antioxid Redox Signal 6:941-3. 2004
  56. ncbi Hemodilution with stroma-free [correction of stoma-free] hemoglobin at physiologically maintained viscosity delays the onset of vasoconstriction
    Géraldine Rochon
    Department of Hematology and Physiology, School of Pharmacy, University Henri Poincaré Nancy, France
    Hypertension 43:1110-5. 2004
    ....