Walter H Watson
Affiliation: Emory University
- Oxidation and nuclear localization of thioredoxin-1 in sparse cell culturesJeanine C Spielberger
Department of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
J Cell Biochem 104:1879-89. 2008....
- Oxidation of nuclear thioredoxin during oxidative stressWalter H Watson
Emory University School of Medicine, Department of Biochemistry, 1510 Clifton Road, Atlanta, GA 30322, USA
FEBS Lett 543:144-7. 2003..Thus, the increase in nuclear Trx1 levels did not correspond to an increase in the overall reducing capacity of Trx1 in the nucleus...
- Redox potential of human thioredoxin 1 and identification of a second dithiol/disulfide motifWalter H Watson
Department of Biochemistry, Emory University, Atlanta, Georgia 30322, USA
J Biol Chem 278:33408-15. 2003....
- Redox state of glutathione and thioredoxin in differentiation and apoptosisWalter H Watson
Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA
Biofactors 17:307-14. 2003
- Compartmentation of Nrf-2 redox control: regulation of cytoplasmic activation by glutathione and DNA binding by thioredoxin-1Jason M Hansen
Department of Medicine and Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
Toxicol Sci 82:308-17. 2004..In contrast, the ARE reporter was principally controlled by nuclear-targeted Trx-1 and not by GSH. The data show that the GSH and TRX systems have unique, compartmented functions in the control of transcriptional regulation by Nrf-2/ARE...
- Glutathione and thioredoxin redox during differentiation in human colon epithelial (Caco-2) cellsYvonne S Nkabyo
Department of Biochemistry, the Graduate Program in Molecular and Systems Pharmacology, Emory University, Atlanta, Georgia 30322, USA
Am J Physiol Gastrointest Liver Physiol 283:G1352-9. 2002..Therefore, the measured 40-mV oxidation of the cellular GSH/GSSG couple or the 28-mV oxidation of the extracellular cysteine/cystine couple should be sufficient to function in signaling or regulation of differentiation in Caco-2 cells...
- Compartmental oxidation of thiol-disulphide redox couples during epidermal growth factor signallingPatrick J Halvey
Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, School of Medicine, Room 225, Emory University, 615 Michael Street, Atlanta, GA 30322, USA
Biochem J 386:215-9. 2005..Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular oxidation...
- Oxidation of the glutathione/glutathione disulfide redox state is induced by cysteine deficiency in human colon carcinoma HT29 cellsLauren T Miller
Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
J Nutr 132:2303-6. 2002..If redox changes occur in vivo in association with variations in dietary Cys and its precursors, these changes could have important physiologic effects through altered redox signaling and control of cell proliferation and apoptosis...
- Thioredoxin and its role in toxicologyWalter H Watson
Division of Pharmacology and Toxicology, College of Pharmacy, The University of Texas at Austin, Austin, Texas 78712, USA
Toxicol Sci 78:3-14. 2004....
- The effects of glutaredoxin and copper activation pathways on the disulfide and stability of Cu,Zn superoxide dismutaseMark C Carroll
Department of Environmental Health Sciences, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
J Biol Chem 281:28648-56. 2006..Overall, these studies implicate a critical balance between cellular reductants such as glutaredoxin and copper activation pathways in controlling the disulfide and stability of SOD1 in vivo...
- Thioredoxin reductase-1 knock down does not result in thioredoxin-1 oxidationWalter H Watson
Johns Hopkins Bloomberg School of Public Health, Department of Environmental Health Sciences, Division of Toxicology, 615 N Wolfe Street, Room E7545, Baltimore, MD 21205, USA
Biochem Biophys Res Commun 368:832-6. 2008..Therefore, TrxR1 inhibition alone was not sufficient to oxidize Trx1, suggesting that Trx1-independent pathways should be considered when evaluating pharmacological and toxicological mechanisms involving TrxR1 inhibition...
- Redox-regulation of Erk1/2-directed phosphatase by reactive oxygen species: role in signaling TPA-induced growth arrest in ML-1 cellsKassim Traore
Department of Environmental Health Sciences, Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, USA
J Cell Physiol 216:276-85. 2008..Together, this study suggested that TPA stimulated ROS generation as a second messenger to activate Erk1/2 via a redox-mediated inhibition of Erk1/2-directed phosphatase in ML-1 cells...